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Aspects on the relief of living surfaces using atomic force microscopy allow “art” to imitate nature
Rosa POLYMENI Emmanuel SPANAKIS Apostolos ARGIROPOULOS Sophia RHIZOPOULOU 《Integrative zoology》2010,5(3):218-225
The visualization of the surface of biological samples using an atomic force microscope reveals features of the external relief and can resolve very fine and detailed features of the surface. We examined specimens from the skin of the amphibians Salamandra salamandra Linnaeus, 1758, Lyciasalamandra luschani basoglui Baran & Atatür, 1980 and Mesotriton alpestris Laurenti, 1768, and from the surface of pollen grains of the plant species Cyclamen graecum Link, 1835 and Cistus salviifolius Linnaeus, 1753, which exhibit certain interesting features, imaged at the nanoscale level. It is likely that the relief influences the attributes of the interfaces between the tissues and the environment. We found that the microsculpture increases in size the surface of the examined tissues and this might be particularly important for their performance in the field. Microsculpturing of amphibians' skin may affect water regulation, dehydration and rehydration, and cutaneous gas exchange. Pollen grain relief might affect the firmness of the contact between pollen surface and water droplets. High resolution imaging of the external relief showed that roughening might induce wetting and influence the water status of the specimens. In addition, roughness affects the radius of water droplets retained in between the projections of the external relief. Roughness of the tissues was highly correlated with their vertical distance, whereas surface distances were highly correlated with horizontal distances. By enabling a more detailed characterization of the external sculptures, through sophisticated techniques, a more comprehensive examination of the samples indicates similarities among different living tissues, originated from different kingdoms, which can be attributed to environmental conditions and physiological circumstances. 相似文献
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Bruno B. Chomel Jennifer B. Henn Rickie W. Kasten Nathan C. Nieto Janet Foley Sophia Papageorgiou Claire Allen Jane E. Koehler 《Veterinary research》2009,40(4)
Bartonella rochalimae was first isolated from the blood of a human who traveled to Peru and was exposed to multiple insect bites. Foxes and dogs are likely natural reservoirs for this bacterium. We report the results of experimental inoculation of two dogs, five cats and six guinea pigs with the only human isolate of this new Bartonella species. Both dogs became bacteremic for 5–7 weeks, with a peak of 103–104 colony forming units (CFU)/mL blood. Three cats had low bacteremia levels (< 200 CFU/mL) of 6–8 weeks’ duration. One cat that remained seronegative had two bacterial colonies isolated at a single culture time point. A fifth cat never became bacteremic, but seroconverted. None of the guinea pigs became bacteremic, but five seroconverted. These results suggest that dogs could be a reservoir of this strain of B. rochalimae, in contrast to cats and guinea pigs. 相似文献
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In this study,we provide the first evidence of two pairs of petals of the rapidly expanded and short-lived nocturnal flowers of Capparis spinosa L.(caper)during the prolonged drought period in Eastern Mediterranean region.The corolla of the winter-deciduous,perennial C.spinosa consists of two pairs of petals:a pair of white distinct petals and a pair of connate variegated petals with green basal parts.The results indicated the presence of substantially different amounts of chlorophyll in the two pairs of petals,while their carbohydrates’content is comparable with that of the green sepals.High resolution imaging of petal surfaces of short-lived flowers of C.spinosa, obtained by using scanning electron microscopy,revealed stomata on the adaxial epidermis on both the white and the green parts of the variegated petals;while dense hairs were found on the surface of the abaxial green parts of the variegated petals.Adaxial,epidermal cells of the variegated petals,viewed using atomic force microscopy,possess a submicron,cuticular microfolding that differs between the white and the green parts of the petals.It appears that microridges on the adaxial,white parts of petals of C.spinosa compensate for an increase in cell surface area of the short lived petals,while the roughness of the green parts of petals was found to be higher than that of the white parts.Thus,the micromorphology of surfaces of epidermal cells is expected to affect optical properties and wettability of the floral tissues.These findings may be particularly important for understanding the performance of the short-lived petals of C.spinosa,which are exposed to dryland environments. 相似文献
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Volker Mohler Rudy Lukman Sophia Ortiz-Islas Manilal William Anthony John Worland Janny van Beem Gerhard Wenzel 《Euphytica》2004,138(1):33-40
Photoperiod response is a major determinant of duration of growing stages in wheat. Conscious selection for these photoperiod
response genes in plant breeding programs will yield genotypes with better adaptation to diverse environments. To provide
a starting point for the development of molecular markers useful for the selection process, genetic maps around the photoperiod
insensitive gene Ppd-B1 were built employing three segregating populations. Of 25 markers that were selected for the Ppd-B1 region, only two could be mapped across all three populations. In pairwise comparisons, the extent of transferable markers
ranged from three to eight. Recombination frequencies of markers distal to Ppd-B1 were more homogeneous than those of proximal markers. This finding suggested a closer proximity of Ppd-B1 to the markers that were mapped distal to breakpoint 0.83 in the physical map of chromosome 2BS.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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Ka Y. Yuen Justine S. Gibson Sophia Hinrichsen Carlos E. Medina-Torres Francois-Rene Bertin Allison J. Stewart 《Journal of veterinary diagnostic investigation》2021,33(1):149
Prudent use of antimicrobials is paramount to slow the development of resistance and for successful treatment. The use of cumulative antibiograms will allow evidence-based antimicrobial selection with consideration of local resistance patterns. We generated a “first-isolate-per-patient” cumulative antibiogram for a regional equine referral hospital. Bacterial organisms cultured from horses between 2011 and 2018, sample origin, antimicrobial susceptibilities, and multidrug-resistant (MDR) status were tabulated. Of 1,176 samples, 50% were culture positive. Overall, 93 of 374 (25%) were MDR. Of the MDR isolates, 11 (12%) were susceptible to high-importance antimicrobials only (as defined by the Australian Strategic and Technical Advisory Group on antimicrobial resistance). β-hemolytic streptococci were uniformly susceptible to penicillin (76 of 76); 17 of 20 (85%) non–β-hemolytic Streptococcus spp. were susceptible to penicillin. Despite veterinary-specific challenges in constructing an antibiogram, our study provides an exemplar of the clinical utility of regional-, farm-, or hospital-specific cumulative antibiograms for evidence-based empirical antimicrobial selection by veterinarians prior to susceptibility result availability. 相似文献
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Plants recognize conserved molecular structures from microorganisms, which triggers active immune responses. Successful pathogens have to overcome this level of immunity; however, plants in turn can adapt their immune system, thus plants and pathogens are in an evolutionary arms race. As being sessile organisms, plants need to integrate and adapt to changing environmental conditions such as light, temperature, drought, or microorganisms. Plants protect themselves against diseases through sensitive recognition of potential pathogens and effective defense systems. The first level of the plant immune system provides recognition of a broad spectrum of microorganisms leading to defense activation (Bittel and Robatzek 2007). The second level of the plant immunity allows certain plant cultivars to detect of specific pathogen strains??a phenomenon also referred to as ??gene-for-gene resistance?? (Jones and Dangl 2006). The first level of immunity occurs rapidly and triggers active defenses normally without harm to the plant cell. The second level of plant immunity develops over days and deploys a local cell death, which prevents pathogens from further spread into tissues. In addition to these cell-autonomous defense systems, plants have also evolved strategies of systemic immunity. 相似文献
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Pierre-Henri Clergeot Claudia Rivetti Mollah Md. Hamiduzzaman Sophia Ekengren 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(4):300-310
Abstract Corky root rot is a ubiquitous soil-borne disease of tomato caused by the pathogen Pyrenochaeta lycopersici. This filamentous fungus is found on the roots of many crops and can persist in the soil up to 15 years as microsclerotia. High prevalence of corky root rot can be partly explained by the endurance and the broad host range of P. lycopersici, but how this fungus can gain access to host roots is still poorly understood, as its competitive saprophytic ability is very low. We have combined microscopy and reporter gene techniques to investigate the tomato–P. lycopersici interaction in vitro, and discovered the pathogen secretes molecules that change the direction of root growth and induce cell necrosis specifically in the apical part of the root of tomato (apex, elongation zone and beginning of the root hair zone). Moreover, we found that the fungus preferentially infects immature root cells that are sensitive to these secreted fungal molecules, whereas infection is blocked in mature and insensitive parts of the root. Our study sheds light on novel and important features of the biology of this pathogen, which could contribute to its fitness in the rhizosphere. 相似文献
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