ARTIFICIAL DREAMS     

Stanley HM 《Science (New York, N.Y.)》1899,9(216):263-264

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Hemoglobin kinetics and the effect of organic phosphates     

Bansil R  Herzfeld J  Stanley HE 《Science (New York, N.Y.)》1974,186(4167):929-932

A kinetic model based on allosteric mechanisms of cooperativity fits the experimentally observed phosphate dependence of hemoglobin reactions. Subunit inequivalence is found to be important in analyzing hemoglobin kinetics. The observed increase in the rate of deoxygenation in the presence of organic phosphates is primarily related to the increased rate of dissociation of the second oxygen molecule.  相似文献   

Medicine. The intangible value of vaccination     

Rappuoli R  Miller HI  Falkow S 《Science (New York, N.Y.)》2002,297(5583):937-939

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Dealing with data: governments records     

Kum HC  Ahalt S  Carsey TM 《Science (New York, N.Y.)》2011,332(6035):1263

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Increased alkalinity in the Mississippi     

Jones JB  Stanley EH  Mulholland PJ 《Science (New York, N.Y.)》2003,302(5647):985-7; author reply 985-7

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AN INTERVAL COMPUTER     

Stanley J 《Science (New York, N.Y.)》1937,86(2241):547-548

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Cell biology. Whither model organism research?     

Fields S  Johnston M 《Science (New York, N.Y.)》2005,307(5717):1885-1886

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Activation of apoptosis in vivo by a hydrocarbon-stapled BH3 helix     

Walensky LD  Kung AL  Escher I  Malia TJ  Barbuto S  Wright RD  Wagner G  Verdine GL  Korsmeyer SJ 《Science (New York, N.Y.)》2004,305(5689):1466-1470

BCL-2 family proteins constitute a critical control point for the regulation of apoptosis. Protein interaction between BCL-2 members is a prominent mechanism of control and is mediated through the amphipathic alpha-helical BH3 segment, an essential death domain. We used a chemical strategy, termed hydrocarbon stapling, to generate BH3 peptides with improved pharmacologic properties. The stapled peptides, called "stabilized alpha-helix of BCL-2 domains" (SAHBs), proved to be helical, protease-resistant, and cell-permeable molecules that bound with increased affinity to multidomain BCL-2 member pockets. A SAHB of the BH3 domain from the BID protein specifically activated the apoptotic pathway to kill leukemia cells. In addition, SAHB effectively inhibited the growth of human leukemia xenografts in vivo. Hydrocarbon stapling of native peptides may provide a useful strategy for experimental and therapeutic modulation of protein-protein interactions in many signaling pathways.  相似文献   

Tritrichomonas foetus infection in purebred cats in Germany: prevalence of clinical signs and the role of co-infection with other enteroparasites     

Kuehner KA  Marks SL  Kass PH  Sauter-Louis C  Grahn RA  Barutzki D  Hartmann K 《Journal of Feline Medicine and Surgery》2011,13(4):251-258

The aim of this study was to determine the prevalence of Tritrichomonas foetus infection and associated clinical signs in purebred cats in Germany, to investigate the role of co-infection, and identify determinants of infection. Faecal specimens accompanied by epidemiological questionnaires were scored and collected from 230 purebred cats. Faeces were examined for trichomonads and other enteroparasites. The prevalence of T foetus was 15.7% among cats and 18.5% among catteries. An abnormal faecal score and history of diarrhoea were observed in 64% and 61% of T foetus-positive cats, respectively, and correlated significantly with infection. Co-infection, observed in 36% of T foetus-infected cats, was not associated with diarrhoea. Norwegian Forest cats were infected significantly more often than other breeds. No association was found with any environmental factors. This study demonstrated a high prevalence of symptomatic T foetus infections in purebred cats in Germany. Co-infection with other enteroparasites did not worsen clinical signs of trichomonosis.  相似文献   

Schirmer tear tests and intraocular pressures in conscious and anesthetized koalas (Phascolarctus cinereus)     

Grundon RA  Anderson GA  Lynch M  Hardman C  O'Reilly A  Stanley RG 《Veterinary ophthalmology》2011,14(5):292-295

Objective To estimate mean Schirmer tear test (STT) and intraocular pressure (IOP) values in healthy koalas both conscious and anesthetized. Methods Data were gathered from koalas in Victoria, Australia. Conscious examinations were performed on captive koalas. Free‐ranging (wild) koalas were examined under anesthesia. Anesthesia was induced using alfaxalone, and animals were maintained on oxygen and isoflurane if required. All animals were healthy and had no surface ocular pathology detectable during slit lamp biomicroscopy. STT I tests were performed using commercial STT test strips placed in the lower fornix for 1 min. IOP was measured using an applanation tonometer after topical anesthesia. The higher value of the two eyes for both STT and IOP was analyzed. STT was measured in 53 koalas (34 conscious, 19 anesthetized) and IOP was measured in 43 koalas (30 conscious, 13 anesthetized). A two‐sample t‐test was used to compare means. A P‐value <0.05 was regarded as significant. Mean ± SD is presented. Results The mean higher STT in conscious koalas was 10.3 ± 3.6 mm wetting/min and in anesthetized koalas it decreased to 3.8 ± 4.0 mm wetting/min (P < 0.0001). The mean higher IOP in conscious koalas was 15.3 ± 5.1 mmHg, and in anesthetized koalas it was 13.8 ± 3.4 mmHg (P = 0.32). There was no effect of sex on either STT or IOP. Conclusions The mean and SD of STT and IOP values for koalas both conscious and anesthetized were reported. The mean STT was significantly reduced by alfaxalone anesthesia.  相似文献