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221.
222.
Malondialdehyde contents in infant milk formulas   总被引:1,自引:0,他引:1  
Malondialdehyde (MDA) levels in infant milk formulas have been monitored by using an aqueous acid extraction method combined with the thiobarbituric acid method (TBA-test). Vegetable oils, with a remarkable content of polyunsaturated fatty acids (PUFA) are used to enrich the infant milk formulas. As PUFA are more susceptible to autoxidation, it becomes of great interest to have information about the safety and preservation of these products. We monitored MDA content in twenty of the most popular infant milk formulas and in some commercial cow milk samples and compared the obtained data. Levels of MDA ranged between 200 and 1200 ppb: all values but one were higher, up to five times, than those found in cow milk samples. To evaluate the accuracy of the data obtained from the TBA-test, some samples were also analyzed with an HPLC derivative method: preliminary results show a good agreement between the two analytical techniques.  相似文献   
223.
SPME was employed to characterize the volatile profile of virgin olive oils produced in two geographical areas of northern Italy: the region of the Gulf of Trieste and the area near Lake Garda. There are as yet no data on the headspace composition of virgin olive oils from these regions, characterized by particular conditions of growth for Olea europaea. Using the SPME technique coupled to GC-MS and GC-FID, the volatile components of 42 industrially produced virgin olive oil samples were identified and the principal compounds quantitatively analyzed. Significant differences in the proportion of volatile constituents from oils of different varieties and geographical origins were detected. The results suggest that besides the genetic factor, environmental conditions influence the volatile formation.  相似文献   
224.
225.
Exploiting the diversity of barley landraces in Ethiopia   总被引:1,自引:0,他引:1  
The paper describes a methodology to incorporate the collection of barley landraces available in the Plant Genetic Resources Centre of Ethiopia (PGRC/E), as well as future collections, in the Ethiopian barley breeding program. Six hundred pure lines extracted from thirty Ethiopian barley landraces were evaluated and selected, along with the original populations, between 1989 and 1993. Three lines were identified which significantly outyielded the local landrace in some of the testing sites and had a higher average yield across sites and seasons. The yield of these lines was similar to the best improved variety also developed from a landrace population. Despite the relatively small number of landrace-lines (600) in the original population, the exploitation of the diversity existing within Ethiopian barley germplasm has produced in only five years promising lines for yield at low input conditions and for disease and insect resistance. Some lines had a yield potential comparable with the best breeding lines produced earlier, and one is under multiplication for release.  相似文献   
226.
The ability of high hydrostatic pressure processing to promote changes in both the structural properties of fiber and the interaction of fiber with water were addressed. Both coarse and fine bran from milling of common wheat were considered. Treatment‐induced morphological changes were most pronounced in fine bran, whereas treatment of coarse bran resulted in the largest change in water‐holding capacity. The significance of the process‐induced changes is discussed in terms of their practical relevance in the production of fiber‐enriched foods.  相似文献   
227.
Olive fruits contain high concentrations of phenols that include phenolic acids, phenolic alcohols, flavonoids, and secoiridoids. The final concentration of phenols is strongly affected by brine conditions. The factors involved in modification by brine are still partially unknown and can include hydrolysis of secoiridoid glucosides and the release of hydrolyzed products. In this study olives from various Italian cultivars were processed by natural fermentation (e.g., without a preliminary treatment of olives with NaOH) using a selected Lactobacillus strain. Processed olives are characterized by a low phenolic concentration of phenols, consisting mainly of phenyl alcohols, verbascoside, and the dialdehydic form of decarboxymethylelenolic acid linked to (3,4-dihydroxyphenyl)ethanol (3,4-DHPEA-EDA), whereas a high level of phenols occurs in olive brine from all the cultivars studied. Olives of the Coratina cultivar, control and with fermentation by Lactobacillus pentosus 1MO, were analyzed in a frozen hydrated state by cryo scanning electron microscopy and energy-dispersive X-ray microanalysis, on both surface and transversal freeze-fracture planes. Structural modifications, found in olives after fermentation, may explain the phenol release in brine.  相似文献   
228.
The time course of compositional changes in the polysaccharide and protein fractions, and of changes in protein structural properties, was investigated in grains of two Italian durum wheat cultivars, Ofanto and Duilio, and the pattern of changes was found to be specific to each cultivar. The fructo-oligosaccharide content in ripening kernels was different between cultivars, as was the pattern of starch accumulation. Evaluation of gluten proteins by Western-blotting using broad-specificity anti-gliadin antibodies showed the sudden appearance of immunoreactive proteins during ripening. Protein surface hydrophobicity and thiol content and accessibility were evaluated in conditions apt at assessing changes in protein interaction and/or in aggregative patterns. Proteins in the two cultivars showed differences in their surface hydrophobicity and in their content of accessible thiols. The simultaneous evaluation of changes in the individual components indicates that use of immature grains for the production of functional foods will require accurate monitoring of the rapidly evolving biopolymer patterns, and careful choice of the cultivar.  相似文献   
229.
The aim of this study was to characterise the performance of new molecular methods for the detection and identification of Pseudomonas syringae pv. actinidiae (Psa) and to provide validation data in comparison to the assays mentioned in official diagnostic protocols and being currently used. Eleven molecular tests for the Psa detection were compared in an inter-laboratory comparison where each laboratory had to analyse the same panel of samples consisting of thirteen Psa-spiked kiwifruit wood extracts. Laboratories had to perform also isolation from the wood extracts. Data from this interlaboratory test performance study (TPS) was statistically analysed to assess the performance of each method. In order to provide complete validation data, both for detection and identification, this TPS was supplemented by a further study of identification from pure culture of phylogenetically closely related Pseudomonas spp., Psa, and bacterial strains associated with kiwifruit. The results of both these studies showed that simplex-PCRs gave good results, whereas duplex-PCR and real-time PCR were the most reliable tools for detection and identification of Psa. Nested and multiplex-PCR gave false-positive results. The use of the most reliable detection test is suggested for routine analyses, but when Psa-free status needs to be accurately assessed, it is recommended that at least two detection tests are used. This work provides a wide comparison of the available diagnostic methods, giving new information for a possible revision of the official diagnostic protocols (e.g. European and Mediterranean Plant Protection Organization (EPPO) protocol PM7/120 for the detection of Psa).  相似文献   
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