Determination and Comparison of the Lipid Profile and Sodium Content of Gluten-Free and Gluten-Containing Breads from the Spanish Market     

Tres  Alba  Tarnovska  Natalia  Varona  Elisa  Quintanilla-Casas  Beatriz  Vichi  Stefania  Gibert  Anna  Vilchez  Elisenda  Guardiola  Francesc 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(3):344-354

Plant Foods for Human Nutrition - The objective is to verify if gluten-free (GF) and gluten-containing (G) breads differ in their sodium content and lipid profile. Samples of GF...  相似文献   

High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MSn) Analysis and Bioactivity Useful for Prevention of “Diabesity” of Allium commutatum Guss     

Loizzo  Monica R.  Tundis  Rosa  Sut  Stefania  Dall’Acqua  Stefano  Ilardi  Vincenzo  Leporini  Mariarosaria  Falco  Tiziana  Sicari  Vincenzo  Bruno  Maurizio 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(1):124-130

Plant Foods for Human Nutrition - This study aimed at evaluating and comparing the chemical profile obtained by HPLC-ESI-MSn analysis, the inhibitory activity of enzymes linked to obesity...  相似文献   

Choice of selection strategy in breeding barley for stress environments   总被引：1，自引：0，他引：1  

Salvatore Ceccarelli  Stefania Grando  Alfredo Impiglia 《Euphytica》1998,103(3):307-318

To determine the optimum selection environment for barley (Hordeum vulgare L.) targeted at low-input, stress environment, barley lines were selected for high yield under stress (YS), high yield under non-stress (YNS), or average yield in stress and non-stress conditions (YA) during three breeding cycles (cohorts) of three years each. The lines were then tested in a total of 21 year-location combinations with average grain yields ranging from 0.35 to 4.86 t ha^-1. Yield under stress of the YS lines was between 27% and 54% higher than that of the YNS lines, with the top YS lines yielding under stress between 16% and 30% more than the top YNS lines. Realized heritability was between 0.35 and 0.67 when selection was conducted under stress and was significant in all three cohorts. By contrast, selection under non-stress gave a significant response in only one cohort, and its efficiency in improving yield under stress was significantly lower than selection under stress. The best YNS line ranked only 19th for yield under stress. The highest-yielding lines under stress were not only selected under stress, but were also landraces collected in very dry areas (< 250 mm total annual rainfall). This confirms earlier findings and supports the idea that the most effective way to improve productivity of crops grown in less-favored areas is to use locally adapted germplasm and select in the target environment(s). This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Suppressive activity of some glucosinolate enzyme degradation products on Pythium irregulare and Rhizoctonia solani in sterile soil     

Luisa M Manici  Luca Lazzeri  Gianluca Baruzzi  Onofrio Leoni  Stefania Galletti  Sandro Palmieri 《Pest management science》2000,56(10):921-926

Glucosinolate degradation products (GLDPs), mainly isothiocyanates, obtained by myrosinase‐catalysed hydrolysis of glucosinolates (GLs), are an important group of natural bioactive substances. The fungitoxic activity of four GLDPs, chosen on the basis of their side‐chain structures, was assayed against Pythium irregulare and Rhizoctonia solani. The effectiveness of the degradation products (DPs) of sinigrin (alkenyl GL), epi‐progoitrin (hydroxy‐alkenyl GL), glucoiberin and glucoerucin (thiofunctionalised GLs) in inhibiting P irregulare oospore germination and R solani soil colonisation were tested in a closed system, using an artificially infected soil. The fungitoxic activity of these GLDPs varied according to their side‐chain structure. As in previous in vitro studies, the thiofunctionalised GLDPs were found the most effective, producing complete inhibition of P irregulare oospore germination (0.01 µmole g⁻¹ soil) and R solani soil colonisation (0.5 µmole g⁻¹ soil), but even sinigrin DP showed a fungitoxic activity higher than that of epi‐progoitrin DP. © 2000 Society of Chemical Industry  相似文献   

Diagnosis of the Compromised Equine Pregnancy     

Stefania Bucca 《Veterinary Clinics of North America: Equine Practice》2006

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Induction of flavonoid production by UV-B radiation in Passiflora quadrangularis callus cultures   总被引：1，自引：0，他引：1  

Antognoni F  Zheng S  Pagnucco C  Baraldi R  Poli F  Biondi S 《Fitoterapia》2007,78(5):345-352

Callus cultures from several species of Passiflora were initiated in vitro, and their capacity to produce four glycosyl flavonoids (orientin, isoorientin, vitexin, isovitexin) was analysed. The aim of the present work was to examine the possible role of UV-B irradiation and elicitation with methyl jasmonate (MJ) on the production of these compounds in callus cultures. All the species tested (P. incarnata, P. quadrangularis, P. edulis) formed friable callus from leaf explants after 4 weeks on medium supplemented with kinetin and 2,4-dichlorophenoxyacetic acid. Among them, P. quadrangularis turned out to have a faster growth rate and a more friable texture, and was therefore chosen for experiments with elicitors. In callus cultures only small amounts of isoorientin were found, while the concentration of the other flavonoids was below the detection limit. UV-B irradiation of calluses was able to increase the production of all four glycosyl flavonoids. After a 7-day exposure of cultures to UV-B light, the production of isoorientin reached concentrations similar to those found in fresh leaves from glasshouse-grown plants. Elicitation with methyl jasmonate also enhanced orientin, vitexin and isovitexin concentrations, even though the stimulation was about 6-fold weaker for orientin and vitexin and about 40-fold for isovitexin, than that exerted by UV-B treatment. Callus cultures treated with the UV-B dose which most enhanced flavonoid production showed a higher antioxidant activity compared to untreated calluses, with an increase ranging from 28% to 76%. Results show that the secondary metabolite biosynthetic capacity of Passiflora tissue cultures can be enhanced by appropriate forms of elicitation.  相似文献   

Histological and micro-/macro-morphological evaluation of intestine in sharpsnout seabream (Diplodus puntazzo) fed soybean meal-based diets added with MOS and inulin as prebiotics     

Ermete Ferrara  Andrea Gustinelli  Maria Letizia Fioravanti  Brunella Restucci  Francesco Quaglio  Stefania Marono  Giovanni Piccolo 《Aquaculture International》2015,23(6):1525-1537

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Ultrastructure of immature and mature human oocytes after cryotop vitrification     

Maria Grazia PALMERINI  Monica ANTINORI  Marta MAIONE  Fabrizio CERUSICO  Caterina VERSACI  Stefania Annarita NOTTOLA  Guido MACCHIARELLI  Mohammad Ali KHALILI  Severino ANTINORI 《The Journal of reproduction and development》2014,60(6):411-420

In vitro maturation of vitrified immature germinal vesicle (GV) oocytes is a promising fertility preservation option. We analyzed the ultrastructure of human GV oocytes after Cryotop vitrification (GVv) and compared it with fresh GV (GVc), fresh mature metaphase II (MIIc) and Cryotop-vitrified mature (MIIv) oocytes. By phase contrast microscopy and light microscopy, the oolemmal and cytoplasmic organization of fresh and vitrified oocytes did not show significant changes. GVv oocytes showed significant ultrastructural alterations of the microvilli in 40% of the samples; small vacuoles and occasional large/isolated vacuoles were abnormally present in the ooplasm periphery of 50% of samples. The ultrastructure of nuclei and mitochondria-vesicle (MV) complexes, as well as the distribution and characteristics of cortical granules (CGs), were comparable with those of GVc oocytes. MIIv oocytes showed an abnormal ultrastructure of microvilli in 30% of the samples and isolated large vacuoles in 70% of the samples. MV complexes were normal, but mitochondria-smooth endoplasmic reticulum aggregates appeared to be of reduced size. CGs were normally located under the oolemma but presented abnormalities in distribution and matrix electron density. In conclusion, Cryotop vitrification preserved main oocyte characteristics in the GV and MII stages, even if peculiar ultrastructural alterations appeared in both stages. This study also showed that the GV stage appears more suitable for vitrification than the MII stage, as indicated by the good ultrastructural preservation of important structures that are present only in immature oocytes, like the nucleus and migrating CGs.  相似文献   

Evaluation of dietary addition of 2 essential oils from Achillea moschata,or their components (bornyl acetate,camphor, and eucalyptol) on in vitro ruminal fermentation and microbial community composition     

Stefania Colombini  Andrea Rota Graziosi  Pietro Parma  Marcello Iriti  Sara Vitalini  Chiara Sarnataro  Mauro Spanghero 《动物营养（英文）》2021,7(1):224

This study investigated the effects of 2 Achillea moschata essential oils extracted from plants collected in 2 different valleys of the Italian Alps and 3 pure compounds of oils — bornyl acetate (BOR), camphor (CAM), and eucalyptol (EUCA) — on in vitro ruminal fermentation and microbiota. An in vitro batch fermentation experiment (Exp. 1) tested the addition of all of the substances (2 essential oils and 3 compounds) in fermentation bottles (120 mL) at 48 h of incubation, whereas a subsequent in vitro continuous culture experiment (Exp. 2) evaluated the pure compounds added to the fermenters (2 L) for a longer incubation period (9 d). In both experiments, total mixed rations were incubated with the additives, and samples without additives were included as the control (CTR). Each treatment was tested in duplicate and was repeated in 3 and 2 fermentation runs in Exp. 1 and 2, respectively. Gas production (GP) in Exp. 1 was similar for all of the treatments, and short chain volatile fatty acid (SCFA) production was similar in both experiments except for a decrease of SCFA produced (P = 0.029) due to EUCA addition in Exp. 2. Compared to CTR, BOR and CAM reduced the valerate proportion (P = 0.04) in Exp. 1, and increased (P < 0.01) the acetate proportion in Exp. 2. All treatments increased (P < 0.01) total protozoa counts (+36.7% and +48.4% compared to CTR on average for Exp. 1 and 2, respectively). In Exp. 1, all of the treatments lowered the Bacteroidetes and Firmicutes and increased the Proteobacteria relative abundances (P < 0.05), whereas in Exp. 2, the EUCA addition increased (P = 0.012) the Ruminococcus. In Exp. 1, methane (CH₄) as a proportion of the GP was lowered (P = 0.004) by the addition of CAM and EUCA compared to CTR, whereas in Exp. 2, EUCA reduced the amount of stoichiometrically calculated CH₄ compared to CTR. Overall, essential oils extracted from A. moschata and the pure compounds did not depress in vitro rumen fermentation, except for EUCA in Exp. 2. In both experiments, an increase of the protozoal population occurred for all the additives.  相似文献   

Characterization of volatiles in different dry gins     

Vichi S  Riu-Aumatell M  Mora-Pons M  Buxaderas S  López-Tamames E 《Journal of agricultural and food chemistry》2005,53(26):10154-10160

A HS-SPME method coupled to GC-MS was developed and applied for the qualitative and semiquantitative characterization of distilled gin volatile fraction. Sampling, chromatographic conditions, and method performances were evaluated, and the developed method was applied as a comparative study of some of the most popular commercial London Dry Gins and other gins with geographic denominations. During this study, 70 components of the gins' volatile fraction were isolated, tentatively identified or identified by reference compounds. They were mainly represented by mono- and sesquiterpenic compounds, which were quantitatively determined. The comparative study of London Dry Gins and gins with geographical indication permitted clear differentiation between the gins with geographical indication and one of the London Dry Gins tested.  相似文献