Feline dysautonomia - the Key-Gaskell syndrome     

Jones BR  Lee EA 《New Zealand veterinary journal》1985,33(4):58

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Infectious bronchitis virus interference with growth of Newcastle disease virus. I. Study of interference in chicken embryos     

L G Raggi  G G Lee 《Avian diseases》1963,7(1):106-122

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The laying performance of two strains of hens offered diets containing dried poultry manure during the laying stage     

D. J. W. Lee  W. Bolton 《British poultry science》1977,18(1):1-7

1. Light‐weight (Babcock B300) and medium‐weight (Warren‐SSL) hens were offered diets, containing 0, 100 or 200 g dried poultry manure (DPM) and 11.49, 11.12 or 10.83 MJ metabolisable energy/kg respectively, between 18 and 70 weeks of age. The true protein, metabolisable energy, calcium and phosphorus contents of the DPM, from caged layer hens, were used in the diet formulation.

2. The hen‐housed laying performance of hens offered the DPM‐containing diets was significantly better for number of eggs laid and total egg mass produced but the performance of the control groups was lower than would normally be expected of those stocks. The reason for the lower rate of lay and higher mortality of hens fed on the control diet was not identified. The amount of food consumed increased as the energy content of the diet decreased and the efficiency of food conversion (EFC) of the lightweight strain was reduced; there was no significant effect on the EFC of the medium‐weight hens.

3. The inclusion of DPM in the diets had no effect on albumen quality or on the incidence of hair‐cracked, cracked or broken eggs. However, with both strains of hens, shell weight and shell thickness were poorer when 100 or 200 g DPM/kg diets were fed.  相似文献   

小麦对条锈病的水平抗病性研究初报   总被引：4，自引：0，他引：4  

曾士迈  王沛有  武修英  张万义  王吉庆  宋位中  王生元 《植物保护学报》1979,6(1):1-10

将小麦品种采用随机区组的田间设计种于小种病圃中,以鉴定和测定其水平抗性。根据各个“品种——小种”组合的相对病情指数的方差分析的结果,有些品种,如农大311、西农6028和丰产3号,其品种——小种互作高度显著,被鉴定为具有垂直抗性,另外一些品种,对试验所用的小种并无专化性,并且在大田生产中已显出20多年的持久的中度抗性,初步推测为属水平抗性。在后一类中,有些品种,如平原50,表现为中等反应型,其余则属于呈典型感病的迟锈慢锈类型。在迟锈慢锈品种的病指的对数矫正值(logits)和病圃的锈病强度(以感病对照品种的病指表之)的对数矫正值之间发现了直线回归。  相似文献   

Feline leukemia virus testing     

Jones BR  Lee EA 《New Zealand veterinary journal》1982,30(10):161

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‘Plantibodies’: a flexible approach to design resistance against pathogens     

A. Schots  J. De Boer  A. Schouten  J. Roosien  J. F. Zil Verentant  H. Pomp  L. Bouwman-Smits  H. Overmars  F. J. Gommers  B. Visser  W. J. Stiekema  J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   

Coccidiosis in chickens recorded in alberta between 1973 and 1977          下载免费PDF全文

Lee EH  Onderka DK  Carlson HC 《The Canadian veterinary journal. La revue veterinaire canadienne》1980,21(8):239-240

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Drug resistance in avian coccidia          下载免费PDF全文

Lee EH 《The Canadian veterinary journal. La revue veterinaire canadienne》1978,19(6):174

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Studies on the chiral isomers of fonofos and fonofos oxon: II. In vitro metabolism     

Philip W. Lee  Reza Allahyari  T.Roy Fukuto 《Pesticide biochemistry and physiology》1978,8(2):158-169

The in vitro metabolism of the chiral isomers of fonofos and fonofos oxon in the presence of mouse liver mixed-function oxidase and serum esterase was investigated. The metabolism of ³⁵S-labeled phenyl-(S)_P-fonofos mediated by mixed-function oxidase took place stereoselectively, resulting predominantly in (R)_P-fonofos oxon. Similarly, (R)_P-fonofos was converted to (S)_P-oxon. In each case, however, a significant amount of racemization occurred. Other products were diphenyl disulfide and diphenyl disulfide oxide. In addition to stereospecificity, the oxidative metabolism of (R)_P-fonofos proceeded at a rate faster than that of (S)_P-fonofos. Stereoselective rate differences also were observed in mouse or rat serum-catalzyed degradation of the fonofos oxon enantiomers, the (S)_P isomer being degraded about twofold faster than its enantiomer. The differences in toxicities of the isomers of fonofos and fonofos oxon were consistent with the in vitro metabolism data.  相似文献   

Statistical study on the predisposition to summer sterility in Holstein bulls born in Hokkaido]     

T Ishikawa  W K Sung 《The Japanese journal of veterinary research》1975,23(2):45-59

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