Immunochromatographic assay for simple and rapid detection of <Emphasis Type="Italic">Satsuma dwarf virus</Emphasis> and related viruses using monoclonal antibodies     

Nario Kusano  Keita Hirashima  Minoru Kuwahara  Kenji Narahara  Tadashi Imamura  Tomohiro Mimori  Ken Nakahira  Kuniaki Torii 《Journal of General Plant Pathology》2007,73(1):66-71

A simple and rapid immunochromatographic assay (ICA) to detect Satsuma dwarf virus (SDV) was developed using colloidal gold conjugates of anti-SDV monoclonal antibodies. Of six homogenization buffers tested, 0.1 M citrate buffer (pH 7.0) gave the best results for the ICA. In the ICA, addition of 0.1% thioglycolic acid in the homogenization buffers that have been widely used in enzyme-linked immunosorbent assays (ELISA) was deleterious to the reaction because of undesirable coagulation of the colloidal gold. ICA using the anti-SDV monoclonal antibodies was 8 times and 16 times more sensitive than double antibody sandwich-ELISA and ICA using the anti-SDV polyclonal antibody, respectively. The analysis is complete in only 15 min. Furthermore, ICA using the anti-SDV monoclonal antibodies could also detect SDV-related viruses.  相似文献   

The TIR–NBS but not LRR domains of two novel N-like proteins are functionally competent to induce the elicitor p50-dependent hypersensitive response     

Jun-Shan Gao  Nobumitsu Sasaki  Hiromi Kanegae  Ken-ichi Konagaya  Kaori Takizawa  Naomi Hayashi  Yosuke Okano  Masahiro Kasahara  Yasuhiko Matsushita  Hiroshi Nyunoya   《Physiological and Molecular Plant Pathology》2007,71(1-3):78-87

The tobacco N protein recognizes the helicase domain (p50) of the Tobacco mosaic virus (TMV) replicase as an elicitor and mediates hypersensitive response (HR). We obtained two cDNA clones encoding novel N-like (NL) proteins NL-C26 and NL-B69 from Nicotiana tabacum cv. Samsun NN. NL-C26 and NL-B69 had a Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR–NBS–LRR) structure and showed 78% and 73% identities to N, respectively. The NL-C26 and NL-B69 genes were also expressed in N. tabacum cv. Samsun nn, which lacks the N gene. Unlike N, NL-C26 and NL-B69, when coexpressed with p50, failed to induce HR on the sites of agroinfiltration in Samsun nn leaves. However, the elicitor-dependent HR in Samsun nn was induced efficiently by chimeric N proteins with the continuous TIR–NBS domains of NL-C26 and NL-B69. On the other hand, the efficiency of HR induction varied significantly among chimeric N proteins with either of the TIR and NBS domains of the NL proteins. In contrast, chimeras carrying the LRR domains of the NL proteins did not induce HR. Thus, the TIR–NBS domains of NL-C26 and NL-B69 could functionally adapt to the LRR domain of N, which may determine the specificity for the elicitor. We speculate that the NL genes are potential HR-inducing resistance genes for undetermined pathogens other than TMV.  相似文献   

Development and characterization of monoclonal antibodies to chicken interleukin-15   总被引：1，自引：0，他引：1  

Min W  Lillehoj HS  Li G  Sohn EJ  Miyamoto T 《Veterinary immunology and immunopathology》2002,88(1-2):49-56

The chicken IL-15 gene was recently cloned and shown to encode a polypeptide with T cell growth factor activity similar to IL-2. To further characterize the chemical and biological properties of chicken IL-15, we generated a panel of monoclonal antibodies against bacterially expressed protein and characterized their binding specificities. All antibodies were reactive by ELISA with recombinant IL-15, but not IL-2, and identified a 15kDa recombinant chicken IL-15 by Western blot analysis. Two antibodies inhibited IL-15-induced proliferation of splenic lymphoblast cells. These monoclonal antibodies will be useful for further structural and immunological studies of chicken IL-15.  相似文献   

A new pyrethroid of high insecticidal activity     

Tadashi Ohsumi  Masachika Hirano  Nobushige Itaya  Yoshio Fujita 《Pest management science》1981,12(1):53-58

New pyrethroids containing heterocyclic rings in the alcohol moieties were synthesised and their insecticidal activities were studied. Of these new pyrethroids, 3-benzylpyrrol-1-ylmethyl (1RS-cis, trans-3(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate was found to be the most effective, having higher activity than permethrin against Musca domestica by a topical application method. In addition, para-substituted analogues of the 3-benzylpyrrol-1-ylmethyl ester were also synthesised to study the substituent effects on insecticidal activities; the residual effects of these compounds against Blattella germanica were also examined.  相似文献   

In vitro studies of controlling tomato puffiness enhanced by temperature,nitrogen source and sucrose concetration     

Takashi Hosoki  Tadashi Asahira 《Scientia Horticulturae》1978,9(4):295-302

Tomato puffiness, which occurs after auxin spray under field conditions, was investigated by using the in vitro fruit-culture technique. Tomato flowers were cultured in different media or at different temperatures for 3 days, to induce parthenocarpy, with 2-hydroxymethyl, 4-chlorophenoxyacetic acid (HCPA) and then parthenocarpic fruits were transferred to media free of HCPA.The higher the temperature during induction of parthenocarpy, the higher the degree of puffiness. Puffiness enhanced at 30°C was corrected by succinic acid-2, 2-dimethylhydrazide (SADH), which lowered the auxin level.High ratio of NO₃-N : NH₄-N in the medium increased the degree of puffiness. Puffiness was enhanced in the medium with NO₃-N alone as the nitrogen source was corrected by (SADH) and by [2-chloroethyl]trimethylammonium chloride (CCC). Different nitrogen levels with a constant ratio of NO₃-N to NH₄-N did not affect the degree of puffiness.High concentrations of sucrose (6 and 8%) in the medium considerably increased the degree of puffiness, but this could also be corrected by CCC and SADH.  相似文献   

Chemical characterization of milk oligosaccharides of the common wombat (Vombatus ursinus)          下载免费PDF全文

Kentaro Hirayama  Epi Taufik  Megumi Kikuchi  Tadashi Nakamura  Kenji Fukuda  Tadao Saito  Keith Newgrain  Brian Green  Michael Messer  Tadasu Urashima 《Animal Science Journal》2016,87(9):1167-1177

Previous structural characterizations of marsupial milk oligosaccharides have been performed in the tammar wallaby, red kangaroo, koala, common brushtail possum and the eastern quoll. To clarify the homology and heterogeneity of milk oligosaccharides among marsupial species, which could provide information on their evolution, the oligosaccharides of wombat milk carbohydrate were characterized in this study. Neutral and acidic oligosaccharides were isolated from the carbohydrate fractions of two samples of milk of the common wombat and characterized by ¹H‐nuclear magnetic resonance spectroscopy. The structures of six neutral saccharides were found to be Gal(β1‐4)Glc (lactose), Gal(β1‐3)Gal(β1‐4)Glc (3'‐galactosyllactose), Gal(β1‐3)Gal(β1‐3)Gal(β1‐4)Glc (3',3”‐digalactosyllactose), Gal(β1‐3)Gal(β1‐3)Gal(β1‐3)Gal(β1‐4)Glc, Gal(β1‐3)Gal(β1‐3)[Gal(β1‐4)GlcNAc(β1‐6)]Gal(β1‐4)Glc (galactosyl lacto‐N‐novopentaose I) and Gal(β1‐3)[Gal(β1‐4)GlcNAc(β1‐6)]Gal(β1‐3)[Gal(β1‐4)GlcNAc(β1‐6)]Gal(β1‐4)Glc (lacto‐N‐novooctaose), while those of six acidic saccharides were Neu5Ac(α2‐3)Gal(β1‐3)Gal(β1‐4)Glc. (sialyl 3'‐galactosyllactose), Neu5Ac(α2‐3)Gal(β1‐3)Gal(β1‐3)Gal(β1‐4)Glc (sialyl 3',3”‐digalactosyllactose), Neu5Ac(α2‐3)Gal(β1‐3)[Gal(β1‐4)GlcNAc(β1‐6)]Gal(β1‐4)Glc (sialyl lacto‐N‐novopentaose a), Gal(β1‐3)[Neu5Ac(α2‐3)Gal(β1‐4)GlcNAc(β1‐6)]Gal(β1‐4)Glc (sialyl lacto‐N‐novopentaose c), Neu5Ac(α2‐3)Gal(β1‐3)Gal(β1‐3)Gal(β1‐3)Gal(β1‐4)Glc,, Neu5Ac(α2‐3)Gal(β1‐3)Gal(β1‐3)[Gal(β1‐4)GlcNAc(β1‐6)]Gal(β1‐4)Glc and Gal(β1‐3)Gal(β1‐3)[Neu5Ac(α2‐3)Gal(β1‐4)GlcNAc(β1‐6)]Gal(β1‐4)Glc. In addition, small amounts of sulfated oligosaccharides but no oligosaccharides containing Neu5Gc or α(2–6) linked Neu5Ac were detected.  相似文献   

Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze‐dried sperm          下载免费PDF全文

Nguyen Thi Men  Kazuhiro Kikuchi  Tadashi Furusawa  Thanh Quang Dang‐Nguyen  Michiko Nakai  Atsunori Fukuda  Junko Noguchi  Hiroyuki Kaneko  Nguyen Viet Linh  Bui Xuan Nguyen  Atsushi Tajima 《Animal Science Journal》2016,87(11):1325-1333

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Acrosome reaction of mouse epididymal sperm on oocyte zona pellucida     

Yamashita M  Yamagata K  Tsumura K  Nakanishi T  Baba T 《The Journal of reproduction and development》2007,53(2):255-262

To improve assessment of the acrosome reaction of mouse epididymal sperm, we employed anti-Izumo1 antibody instead of antibodies against acrosomal proteins. The acrosomal states among acrosome-intact, spontaneously acrosome-reacted, truly acrosome-reacted, and probably dead and/or membrane-damaged sperm were clearly distinguished by combined application of anti-Izumo1 antibody, DNA dye Hoechst 33342, and monoclonal antibody MN7 to paraformaldehyde-fixed sperm. When the acrosome reaction of capacitated epididymal sperm on the oocyte zona pellucida was examined using anti-Izumo1 antibody, approximately 20% of sperm bound onto the zona pellucida were acrosome-reacted 30 min after insemination. We also observed the moment of the acrosome reaction of live sperm on the zona pellucida by time-lapse monitoring using fluorescein isothiocyanate-conjugated anti-Izumo1 antibody.  相似文献   

Effect of fat content on sensory characteristics of marbled beef from Japanese Black steers          下载免费PDF全文

Fumiko Iida  Kaoru Saitou  Tadashi Kawamura  Shizuko Yamaguchi  Toshihide Nishimura 《Animal Science Journal》2015,86(7):707-715

To analyze the sensory characteristics of meat samples with a crude fat content between 23.8% and 48.6% taken from 34 Japanese Black steers, we grilled the meat and subjected it to analytical sensory evaluation. We also measured the amounts of moisture, protein, nucleic acid and glutamic acid. An increase in crude fat content increased the tenderness, juiciness, and fattiness in the meat quality evaluation. An increase in crude fat content reduced the crude protein and moisture contents; it also slightly reduced the nucleic acid and glutamic acid contents, although when the reductions in these umami components were assessed relative to the moisture content they changed little. Increasing the fat content up to a certain point greatly enhanced the umami intensity and beef flavor intensity in the meat quality evaluation and raised the overall evaluation score; the peak of the appropriate crude fat content for these purposes was about 36%.  相似文献   

Development of an assay system using thrips-mediated inoculation to evaluate resistance of Capsicum spp. to Tomato spotted wilt virus     

Yoshikatsu Genda  Shinya Tsuda  Osamu Nunomura  Tadashi Ito 《Journal of General Plant Pathology》2008,74(2):171-175

Considerable losses in pepper production by Tomato spotted wilt virus (TSWV) have been reported worldwide. In breeding programs, an assay for resistance that accurately estimates field occurrence of TSWV during pepper production is critical because the virus is vector transmitted. Here, we establish an assay system of TSWV-resistant Capsicum spp. using insect-mediated inoculation within an acrylic chamber in which environmental conditions such as temperature, light intensity, and nutrient supply are controlled. This chamber enables transmission of TSWV from viruliferous plants to plants used in the resistance assay with Frankliniella occidentalis safely, quickly, and precisely.  相似文献