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351.
In vitro formation of holes on the inner perivitelline layer of quail ovum by chicken spermatozoa 总被引:2,自引:0,他引:2
Mar Mar WIN Yuki MIYAGI Koji ASHIZAWA Hideki TATEMOTO Tadashi NAKADA 《Animal Science Journal》2005,76(2):133-138
The aim of the present study was to examine whether chicken semen can be substituted for quail semen to conduct in vitro experiments on fertilization. Chicken spermatozoa was incubated with the inner perivitelline layer (IPL) isolated from the largest follicle in the quail ovary under in vitro conditions. The perforation of chicken and quail spermatozoa were assessed by counting the number of all visible holes in the pieces of IPL. No difference was found in the number of holes formed by the chicken sperm and quail IPL interaction compared with that between intraspecies. In addition, the number of holes in the IPL was significantly increased with the increase in sperm concentration from 1 × 106 to 8 × 106 sperm/mL (P < 0.05). Interestingly, after treatment of chicken spermatozoa with 2.5 mg/mL of the solubilized quail IPL followed by incubation with the intact chicken IPL, the number of holes in the intact chicken IPL was significantly decreased as compared with that of spermatozoa treated without the solubilized IPL (P < 0.05). This indicates that sperm receptors in the solubilized quail IPL and binding ligands on the chicken spermatozoa would find each other and bind, forming complexes and these complexes blocked the interaction between chicken spermatozoa and intact chicken IPL. These results show that: (i) chicken spermatozoa possess the penetrability into quail IPL; and (ii) a high degree of affinity via the receptor interactions exists between chicken spermatozoa and quail IPL. Therefore, it appears that the substitution of chicken semen for quail semen is possible to use as an in vitro technique to examine the sperm‐IPL interaction during fertilization in quail. 相似文献
352.
Bo-Song Ryang Tadashi Matsumoto Takashi Kobori Yoshitaka Kosaka Satoshi T. Ohki 《Journal of General Plant Pathology》2005,71(4):308-313
Cucumber cotyledons inoculated with Cucumber mosaic virus (CMV, Pepo strain) or Zucchini yellow mosaic virus (ZYMV, Z5-1 isolate) developed either mild chlorotic spots or no symptoms. Cotyledons treated with CMV plus ZYMV also developed mild chlorotic spots. However, plants ZYMV-inoculated cotyledons had veinal yellowing and gradual cell death by 20 days postinoculation (dpi) when co-inoculated with CMV on the other cotyledon. When analyzing this synergism, an enzyme-linked immunosorbent assay showed that CMV gradually increased in CMV-inoculated cotyledons of plants, with the other cotyledon mock- or ZYMV-inoculated. However, CMV significantly increased at 9 to 14 dpi in the ZYMV-inoculated cotyledons of plants co-infected with CMV. ZYMV similarly increased in cotyledon pairs of both co-infected and singly infected plants. Inoculation with PepoΔ2b, a modified Pepo-CMV that lacks translation of the 2b protein, revealed that PepoΔ2b without the 2b protein systemically infected cucumber but induced no symptoms on cotyledons or true leaves. Plants with a ZYMV-inoculated cotyledon and co-infected with PepoΔ2b did not undergo cell death; nevertheless, PepoΔ2b was at high levels comparable to levels of CMV in the ZYMV-inoculated cotyledon. The 2b protein thus seems essential for induction of the novel gradual cell death in ZYMV-inoculated cotyledons of cucumbers co-infected with CMV. 相似文献
353.
Keiko MINATO Yasuyuki KOUDA Masaaki YAMAKAWA Satoshi HARA Tadashi TAMURA Takashi OSADA 《Animal Science Journal》2013,84(2):165-177
We developed a system for measuring emissions from stored slurry by using a floating dynamic chamber. CH4, CO2, N2O and NH3 emitted from the storage tank of a dairy cattle farm in eastern Hokkaido were measured during summer 2008 (7/16–8/6), fall 2008 (10/2–10/26), spring 2009 (6/2–6/21) and winter 2009 (3/11). Average daily gas emission rates in summer, fall and spring were, respectively, 54.8, 54.2 and 34.3 g/m2 for CH4; 602, 274 and 254 g/m2 for CO2; 55.4, 68.2 and trace mg/m2 for N2O; and 0.55, 0.73 and 0.46 g/m2 for NH3. CH4, CO2 and NH3 emission rates during the brief measurement period in winter were reduced to 1/4, 1/23 and 1/2, respectively, of summer emission rate levels. All gas emissions showed diurnal fluctuation and were greatest during the daytime, when the ambient temperature rose. CH4, NH3 and CO2 emissions increased significantly during the daytime, and the daily emission (in grams) of each gas was positively correlated with maximum daily temperature. According to the combined spring, summer and fall measurements, the CH4, N2O and NH3 annual emission factors were 1.42% (g CH4/g volatile solids), 0.02% (g N2O‐N/g total N) and 0.43% (g NH3‐N/g total N), respectively. 相似文献
354.
355.
Tadashi Abe Yasunori Nonoue Nozomi Ono Motoyasu Omoteno Masato Kuramata Shuichi Fukuoka Toshio Yamamoto Masahiro Yano Satoru Ishikawa 《Breeding Science》2013,63(3):284-291
To advance the identification of quantitative trait loci (QTLs) to reduce Cd content in rice (Oryza sativa L.) grains and breed low-Cd cultivars, we developed a novel population consisting of 46 chromosome segment substitution lines (CSSLs) in which donor segments of LAC23, a cultivar reported to have a low grain Cd content, were substituted into the Koshihikari genetic background. The parental cultivars and 32 CSSLs (the minimum set required for whole-genome coverage) were grown in two fields with different natural levels of soil Cd. QTL mapping by single-marker analysis using ANOVA indicated that eight chromosomal regions were associated with grain Cd content and detected a major QTL (qlGCd3) with a high F-test value in both fields (F = 9.19 and 5.60) on the long arm of chromosome 3. The LAC23 allele at qlGCd3 was associated with reduced grain Cd levels and appeared to reduce Cd transport from the shoots to the grains. Fine substitution mapping delimited qlGCd3 to a 3.5-Mbp region. Our results suggest that the low-Cd trait of LAC23 is controlled by multiple QTLs, and qlGCd3 is a promising candidate QTL to reduce the Cd level of rice grain. 相似文献
356.
Kanako OYAMA Tadashi BABA Shin-ichi KASHIWABARA 《The Journal of reproduction and development》2021,67(1):35
Testis-brain RNA-binding protein (TB-RBP/Translin) is known to contribute to the translational repression of a subset of haploid cell-specific mRNAs, including protamine 2 (Prm2) mRNA. Mutant mice lacking TB-RBP display abnormal spermatogenesis, despite normal male fertility. In this study, we carried out functional analysis of TB-RBP in mammalian cultured cells to understand the mechanism of translational repression by this RNA-binding protein. Although the amino acid sequence contained a eukaryotic translation initiation factor 4E (EIF4E)-recognition motif, TB-RBP failed to interact with EIF4E. In cultured cells, TB-RBP was unable to reduce the activity of luciferase encoded by a reporter mRNA carrying the 3’-untranslated region of Prm2. However, λΝ-BoxB tethering assay revealed that the complex of TB-RBP with its binding partner, Translin-associated factor X (TRAX), exhibits the ability to reduce the luciferase reporter activity by degrading the mRNA. These results suggest that TB-RBP may play a regulatory role in determining the sequence specificity of TRAX-catalyzed mRNA degradation. 相似文献
357.
Chungyu PAI Shunya NAKAYAMA Yasuyo ITO-FUJISHIRO Kiichi KANAYAMA Yoshiko MUNESUE Tadashi SANKAI Yasuhiro YASUTOMI Hiroshi KOIE Naohide AGEYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):716
Nonhuman primates are commonly used as experimental animals due to their biological resemblance to humans. In patients with cardiac disease, the levels of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) tend to increase in response to cardiac damage, and they are thus used as indicators for the diagnosis of human heart failure. However, no reference values for ANP and BNP have been reported for heart disease in nonhuman primates. In this study, we recorded the age, sex, and body weight of 202 cynomolgus monkeys, and performed evaluations to assess the ANP and BNP levels, electrocardiography and echocardiography, and accordingly divided the monkeys into two groups: healthy monkeys and those with spontaneous cardiac disease. Statistical analysis was performed to determine the relationship of ANP and BNP with the factors of age, sex, and body weight. No significant relationship was found between the levels of ANP and BNP and the factors of age, sex, and body weight. However, both the ANP and BNP levels were significantly different between the healthy monkeys and monkeys with valvular disease. Similar to humans, the ANP and BNP levels tended to increase with the progression of cardiac disease in monkeys. Based on these results, we concluded that ANP and BNP are indicators of cardiac disease in nonhuman primates, and that this nonhuman primate cardiac disease model is applicable for cardiology research in humans. 相似文献
358.
359.
Effects of drag coefficient of netting for dynamic similarity on model testing of trawl nets 总被引:1,自引:0,他引:1
SUMMARY: For design or improvement of fishing nets, a model test based on Tauti's law or Froude's law is well executed. However, because these modeling rules are based on the proportion of the drag of the net to the square of water velocity, the converted drag of the net from the result of model testing is considerably large compared with the observed value. In this study, Tauti's law and Froude's law were corrected considering the drag coefficient of the net to the Reynolds number based on the twine diameter used experimentally. Three different scale models: 1/12, 1/20 and 1/50 of a midwater trawl net of 61 m in total length were made according to the modified Tauti's law and Froude's law, and the full-scale and model experiment were carried out. Consequently, the difference between the converted drags of full-scale by three scale models and measured values was within 10%. Each of these modified modeling rules is effective for the model test of trawl nets. 相似文献
360.