全文获取类型
收费全文 | 362篇 |
免费 | 7篇 |
国内免费 | 1篇 |
专业分类
林业 | 48篇 |
农学 | 11篇 |
76篇 | |
综合类 | 17篇 |
农作物 | 14篇 |
水产渔业 | 63篇 |
畜牧兽医 | 115篇 |
园艺 | 7篇 |
植物保护 | 19篇 |
出版年
2022年 | 1篇 |
2021年 | 9篇 |
2020年 | 4篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 5篇 |
2016年 | 12篇 |
2015年 | 9篇 |
2014年 | 10篇 |
2013年 | 49篇 |
2012年 | 9篇 |
2011年 | 18篇 |
2010年 | 7篇 |
2009年 | 9篇 |
2008年 | 12篇 |
2007年 | 30篇 |
2006年 | 25篇 |
2005年 | 38篇 |
2004年 | 19篇 |
2003年 | 26篇 |
2002年 | 18篇 |
2001年 | 24篇 |
2000年 | 12篇 |
1999年 | 3篇 |
1998年 | 3篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1981年 | 2篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1970年 | 1篇 |
1967年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有370条查询结果,搜索用时 31 毫秒
361.
Two lines of hexaploid wheat were crossed and the basic generations of parent, F1, F2 and back-cross were sown in a controlled-environment
chamber. FreshF1 and back-cross grains were generated, so the material could be handled either as the standard set of basic
generations on a whole-plant basis, or as an extended set on an embryo or endosperm basis. The experiment was repeated. Mature
grains were harvested and the starch particle size distribution was analysed in 3284 grains from 111 plants. Means and variances
were partitioned into additive, dominance and interaction components. Grains from cross-pollinations had B-granule contents
between parental values, rather than of the maternal parent, indicating an involvement of the grain genotype. Quantitative
models based on endosperm genotype gave a better fit to the data than those based on embryo genotype. The difference in starch
B-granule content between the parents was largely due to additive genes. Dominant genes were also indicated, with the first
dose in the triploid endosperm having a large effect while the second dose had little or none. Non-allelic interactions were
significant in the second experiment where the use of more types of backcross made them more detectable. There were also small
and significant residual effects of the maternal plant in the first experiment, attributed to the vigour of the F1 mother
plant and to the cytoplasm of Sunco. Narrow-sense heritability was low, between 0.05and 0.18 depending on the generation.
Transgressive segregation was not found, suggesting that all alleles tending to increase the B-granule content were found
in the Sunco parent and none in ME71. There was also no detectable heterosis in this character. The results show that breeding
and selection for a low B-granule content should be possible but a further reduction will require new and complementary genes.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
362.
Eishiro Okamoto Hitoshi Kasahara Akira Chiba Masayuki Taniguchi Eiichi Saitoh 《Fisheries Science》2009,75(2):463-471
We have discovered multiple acidic cysteine protease inhibitors, in addition to the known Eel-CPI-1, in the skin mucus extract
of the Japanese eel Anguilla japonica by using the two-dimensional gel system of gelatin reverse zymography. Two of the acidic inhibitors, which we have named
Eel-CPI-2 and Eel-CPI-3, were purified to homogeneity by anion exchange chromatography on a column of DEAE-Sepharose CL-6B,
followed by fast protein liquid chromatography on Superdex 75 10/300 GL and HiTrap Q HP columns. The amino acid compositions
of Eel-CPI-2 and Eel-CPI-3 were found to be almost identical and closely similar to that of the eel galectin AJL1. The molecular
masses of Eel-CPI-2 and Eel-CPI-3 were elucidated to be 16,089.080 and 16,089.137 Da, respectively, by matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry. The apparent dissociation constant of Eel-CPI-2 and Eel-CPI-3 for
cysteine protease papain was determined to be 1.79 × 10−7 and 1.05 × 10−7 M, respectively, by a quartz crystal microbalance technique. 相似文献
363.
Miyuki Iizuka Yasuhiro Arima Tadashi Yokoyama Koichiro Watanabe 《Soil Science and Plant Nutrition》2013,59(2):219-225
Sufficient rhizobium population in the rhizosphere of legume seedlings is required for early and enough setting of root nodules. Potential of seed and seedling root exudates for proliferation of Bradyrhizobium japonicum PNT119 was evaluated in the soybean cvs. Enrei and Tachinagaha. In both cultivars, seed exudates showed a higher potential than root exudates. In the seed exudates, a low-molecular high-polarity fraction including sugars and amino acids showed a higher potential than both high-molecular fraction and low-molecular low-polarity fraction. The correlations between the sugar or amino acid contents of seed exudates and the number of root nodule primordia at 7 d after sowing were investigated among 12 soybean cultivars. When seeds were inoculated with a low rhizobium density, a high correlation coefficient was detected between them. However, there was no positive correlation in the plants inoculated with a high rhizobium density. These results suggest that the amount of low molecular substances in the seed exudates determines the number of root nodule primordia through rhizobium proliferation around seed and young roots when the rhizobium density is the limiting factor. The genetic trait relating to seed coat secretion should be considered as a possible key factor contributing to adequate root nodulation in soybean seedlings cultivated in fields with a low rhizobium density. 相似文献
364.
Minori Miyatake Takuji Ohyama Tadashi Yokoyama Soh Sugihara Takashi Motobayashi Takehiro Kamiya 《Soil Science and Plant Nutrition》2013,59(3):259-266
Sulfur (S) and Nitrogen (N) metabolisms in plants are interacted and it is known that S deficiency decrease N absorption and metabolism. In leguminous plants S deficiency also decreases N2 fixation by rhizobia in the nodules. Deep placement of a controlled-release N fertilizer is a good method to provide nitrogen to soybean without inhibiting N2 fixation; thus, it was hypothesized that this method is able to provide nitrogen effectively to sulfur-deficient soybean plants. In this study effects of deep placement of coated urea on S-N physicological interaction, growth and productivity in soybean plants were examined using pot experiments. Soybean plants were grown with sulfate concentrations of 30, 100, or 1000 μM, with or without deep placement of coated urea. Shoot weights at the developing stage were not affected by S deficiency. SPAD values of leaves during the flowering stage decreased with S deficiency and increased with the deep placement of coated urea. S deficiency decreased seed weight per plant at the harvesting stage, but this decrease was attenuated by the deep placement of coated urea. N and S content in shoots at the developing stage increased with the deep placement of coated urea, whereas in seeds, only the N content increased. N2 fixation activity based on the relative ureide-N content in xylem sap indicated that the deep placement of coated urea did not inhibit N2 fixation activity at the early flowering stage. Without deep placement of coated urea, the relative ureide-N content decreased under S deficiency at the seed filling stage. These results suggest that the deep placement of coated urea is an efficient method to supply N to support soybean yield under S deficiency.Abbreviations: Deep+: with deep placement of coated urea; Deep–: without deep placement of coated urea 相似文献
365.
Keiichi?UchidaEmail author Nobuaki?Arai Kazuyuki?Moriya Yoshinori?Miyamoto Toshiharu?Kakihara Tadashi?Tokai 《Fisheries Science》2005,71(5):992-1002
ABSTRACT: In this study, we developed a new automatic system, comprised of a radio frequency identification (RFID) system and global positioning system (GPS), for monitoring fishing effort, and effectiveness of effort, in fisheries using many pieces of fishing gear, such as hooks of longlines. The outline of this system is as follows. A single RFID tag with an identification (ID) number is attached to each piece of fishing gear. The RFID tag on the fishing gear passes the antenna of the RFID reader before being shot into the sea and after being hauled up from the sea. Data on the time and geographic location are measured by the GPS and recorded along with the ID number in a personal computer (PC). When a fish is caught, it is brought close to the fish-sorting table. Then, the second antenna of the RFID reader set at the side checks for a catch in the gear. The advantage of this system is that the fishing operation data can be collected without interfering with the fishermen's usual work. The prototype of the system was tested at a conger-eel tube fishery in Tokyo Bay and was able to record data on the time, the location and the catch of individual conger-eel tubes successfully. 相似文献
366.
Yan Tan Kiyoshi Osatomi Yukinori Nozaki Tadashi Ishihara Kenji Hara 《Fisheries Science》2006,72(1):185-194
ABSTRACT: We purified cathepsins B1 and B2 from the ordinary muscle of carp Cyprinus carpio . The N-terminal amino acid sequences (12 residues) of 29 kDa bands of cathepsins B1 and B2 are the same and showed high homology of 75% and 83%, respectively, with the heavy chain of rat and human cathepsins B. Based on conserved sequences of other cathepsins B and the N-terminal amino acid sequences of 29 kDa bands, we cloned carp cathepsin B cDNA. The nucleotide sequence of carp cathepsin B cDNA consists of 1470 bp including a 993 bp open reading frame, encoding a deduced protein of 330 amino acids. The deduced amino acid sequence of carp cathepsin B has similarity of 80% to rainbow trout cathepsin B and of 76–78% to other vertebrate cathepsins B. The sequence of its isoform was also determined during molecular cloning, which has 94.8% similarity with first cloned cathepsin B. They are completely same in N-terminal amino acid sequence of heavy chain, active site and potential N-glycosylation site. This indicates there are at least two kinds of cathepsin B functioning in vivo in carp. 相似文献
367.
Norishige Yotsukura Tadashi Kawai Taizo Motomura Terunobu Ichimura 《Fisheries Science》2001,67(5):857-862
ABSTRACT: Random amplified polymorphic DNA (RAPD) was studied to detect genetic markers for three economically important Japanese laminarian species, Laminaria japonica Areschoug, L. religiosa Miyabe and L. ochotensis Miyabe, which were sampled from their representative localities on the coasts from Tsugaru Straits to the Sea of Japan. DNA templates for RAPD were extracted from lamina using a DNA extraction kit and were purified with glassmilk. Reproducible RAPD markers for these three species were detected using three random primers from a total of 15 tested. In L. japonica and L. religiosa , these RAPD markers were confirmed to be useful for populations from other localities. The three species studied showed high intraspecific and interspecific band sharing index (BSI) values. Like annual typical L. religiosa from other localities, biennial individuals identified as putative L. religiosa from Atsuta could be discriminated from L. japonica but not from L. ochotensis by any of the RAPD genetic markers studied so far. 相似文献
368.
Michiru Shiga Daisuke Shiode Satoshi Hayashi Tadashi Tokai Fuxiang Hu 《Fisheries Science》2008,74(3):479-487
ABSTRACT: A midwater float is a small float attached to the mainline of pelagic longlines to standardize the hook depth. In this study theoretical equations are presented for estimating the buoyancy of the midwater float required to lift the joints of the midwater float line on the mainline to the target depth. Sea trials using full scale tuna longline gear with midwater floats were carried out in the Indian Ocean in December 2004 and 2005, in order to examine the validity of the theoretical equations. In the sea trials, two types of midwater float settings, single midwater float setting and double midwater float setting, were tested and compared with the conventional setting. As a result, the joints of the midwater float line on the mainline were successfully lifted to the target depth as expected, demonstrating the validity of the theoretical equation. The range of hook depths in the midwater float setting was less spread over depths than in the conventional setting, and therefore, use of long float lines (100 m) with the midwater float setting allows all hooks to avoid entering the sea turtle habitat of shallower than 100 m depth. Factors affecting shoaling of the longline with the midwater float are also discussed. 相似文献
369.
Zenta Nishio Hanaki Oikawa Takanobu Haneda Masako Seki Miwako Ito Tadashi Tabiki Hiroaki Yamauchi Hideho Miura 《Cereal Chemistry》2009,86(3):313-318
Reduced amylose wheat (Triticum æstivum L.) produces better quality noodles and bread less prone to going stale, while little is known about the relationships between amylose content and the quality of soft wheat baking products such as sugar snap cookies (SSC) and Japanese sponge cakes (JSC). Near‐isogenic lines developed from wheat cultivar Norin 61, differing in their level of granule‐bound starch synthase (Wx protein) activity, were used to produce wheat grains and ultimately flours of different amylose contents. These were tested with regard to their effect on soft wheat baking quality and solvent retention capacities (SRC). Amylose content was strongly correlated to cookie diameter (r = 0.969, P < 0.001) and cake volume (r = 0.976, P < 0.001), indicating that the soft wheat baking quality associated with SSC diameter and JSC volume were improved by an incremental increases in amylose content. Among the four kinds of SRC tests (water, sodium carbonate, sucrose and lactic acid), the water SRC test showed the highest correlation with amylose content, SSC diameter, and JSC volume. When the regression analysis was conducted between the nonwaxy and partial waxy isogenic lines that are available in commercial markets, only water SRC was significantly correlated to amylose content (r = –0.982, P < 0.001) among of four SRC tests. This suggests that, unlike udon noodle quality, high‐amylose content is indispensable in improving soft wheat baking quality, a process requiring less water retention capacity. 相似文献
370.
Zenta Nishio Yuri Miyazaki Masako Seki Miwako Ito Tadashi Tabiki Koichi Nagasawa Hiroaki Yamauchi Hideho Miura 《Cereal Chemistry》2011,88(2):189-194
The effect of growing environments of soft wheat on amylose content and its relationship with baking quality and solvent retention capacities (SRC) was investigated. Near‐isogenic soft wheat lines of Norin 61 differing in granule‐bound starch synthase (Wx protein) activity and grown in three different regions of Japan: Hokkaido (spring‐sown) for 2006 and 2007, Kanto (autumn‐sown), and Kyushu (autumn‐sown) for 2007 were evaluated. Spring‐sown samples produced grains of greater protein content (10.9–12.4%) than autumn‐sown samples (7.3–9.1%). In contrast, spring‐sown samples of 2007 with higher maturing temperature had lower amylose content (25.5% for Norin 61) compare to the autumn‐sown and spring‐sown samples of 2006 (27.6–28.4% for Norin 61). Amylose content was strongly correlated to sugar snap cookie (SSCD) diameter (r = 0.957–0.961; n = 10, all samples; P ≤ 0.001, r = 0.701–0.976; n = 7 partial waxy and nonwaxy samples; and Japanese sponge cake (JSCV) volume r = 0.971–0.993; n = 10; P≤ 0.001, r = 0.764–0.922; n = 7 partial waxy and nonwaxy samples), regardless of seeding season and growing conditions. The strength of the JSVC‐amylose relationship (slope) was similar among the three regions, whereas the strength of the SSCD‐amylose relationship was slightly weaker for spring‐sown samples and slightly stronger for partial waxy and nonwaxy autumn‐sown samples. Among of the four solvents (water, solutions of sodium carbonate, sucrose, or lactic acid), water‐SRC showed the greatest correlation to amylose content (r = –0.969 to –0.996; n = 10; P ≤ 0.001, r = –0.629 to –0.983; n = 7 partial waxy and nonwaxy samples), indicated that amylose content can be accurately estimated from the water‐SRC within the samples from the same grown environment. 相似文献