Follicular Thyroid Carcinoma Characterized by Abundant Stromal Components with Chondroid and Osseous Metaplasia in a Dog     

Ryosuke KOBAYASHI  Naoaki YAMADA  Takashi KITAMORI  Fumiyo KITAMORI  Kazunari SATO  Takuya DOI  Yumi WAKO  Junko SATO  Minoru TSUCHITANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1161-1164

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Localization of Eimeripain,an Eimeria tenella Cathepsin B-Like Cysteine Protease,during Asexual and Sexual Intracellular Development in Chicken Ceca     

Makoto MATSUBAYASHI  Takeshi HATTA  Takeharu MIYOSHI  ANISUZZAMAN  Kazumi SASAI  Kayoko YAMAJI  Kameo SHIMURA  Takashi ISOBE  Kiyoshi KITA  Naotoshi TSUJI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):531-537

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Ovarian follicular dynamics and hormones throughout the estrous cycle in Thai native (Bos indicus) heifers     

Jakkhaphan Chasombat  Takashi Nagai  Rangsun Parnpai  Thevin Vongpralub 《Animal Science Journal》2014,85(1):15-24

Relatively few studies have been reported regarding the reproductive physiology of female Thai native cattle. Therefore, the objective of the present study was to evaluate the follicular dynamics and concentrations of follicle stimulating hormone (FSH), estradiol (E2) and progesterone (P4) during the estrous cycle in Thai native heifers (TNH) and to compare obtained results with those of European and Indian cattle breeds previously reported. For the detection of estrus, ovaries of all 20 heifers were examined twice daily (12 h intervals) by ultrasonography for three consecutive estrous cycles. From data of 60 estrous cycles (n = 60 estrous cycles from 20 heifers), it was found that 14 (70%) and 6 heifers (30%) had two (42 estrous cycles collected from 14 heifers) and three follicular waves (18 estrous cycles collected from 6 heifers), respectively. The days when estrus was detected, interovulatory intervals, life‐spans of corpus lutea (CL), and days for growing and regression of CLs were shorter in the two follicular waves than those in the three follicular waves (P < 0.05). In both two and thre follicular waves, larger maximum diameters and higher growth rates of the dominant follicle (DF) in an ovulatory wave were observed than those of the preceding waves without ovulation (P < 0.05). There was a progressive increase in follicular size and FSH and E2 production during follicular growth in each follicular wave. In addition, the FSH and E2 peak concentrations during the ovulatory wave were higher than those of the anovulation waves (P < 0.05). Moreover, although the ovarian follicular dynamic patterns in Thai native heifers were similar to those previously reported for European and Indian cattle breeds, the diameter of the largest preovulatory follicle (OF), subordinate follicles (SF) and CLs were smaller than those in European and Indian cattle breeds. In conclusion, when compared with European and some breeds of Indian cattle, the length of interovulatory intervals was shorter, and the sizes of dominant SF and CLs were smaller in Thai native heifers.  相似文献   

Anti-inflammatory and Intestinal Barrier–protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds     

Soichi TANABE  Takuya SUZUKI  Yuichiro WASANO  Fumihiko NAKAJIMA  Hiroshi KAWASAKI  Tomonori TSUDA  Natsuko NAGAMINE  Takashi TSURUMACHI  Kiyoshi SUGAYA  Hiroaki AKITA  Misako TAKAGI  Kuniaki TAKAGI  Yoshinobu INOUE  Yo ASAI  Hidetoshi MORITA 《Journal of Equine Science》2014,25(2):37-43

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Isolation of chicken taste buds for real‐time Ca2+ imaging     

Ken‐ichi Kudo  Fuminori Kawabata  Toumi Nomura  Ayumi Aridome  Shotaro Nishimura  Shoji Tabata 《Animal Science Journal》2014,85(10):904-909

We isolated chicken taste buds and used a real‐time Ca²⁺ imaging technique to investigate the functions of the taste cells. With RT‐PCR, we found that isolated chicken taste bud‐like cell subsets express chicken gustducin messenger RNA. Immunocytochemical techniques revealed that the cell subsets were also immunopositive for chicken gustducin. These results provided strong evidence that the isolated cell subsets contain chicken taste buds. The isolated cell subsets were spindle‐shaped and approximately 61–75 μm wide and 88–98 μm long, and these characteristics are similar to those of sectional chicken taste buds. Using Ca²⁺ imaging, we observed the buds' response to 2 mmol/L quinine hydrochloride (a bitter substance) and their response to a mixture of 25 mmol/L L‐glutamic acid monopotassium salt monohydrate and 1 mmol/L inosine 5′‐monophosphate disodium salt, umami substances. The present study is the first morphological demonstration of isolated chicken taste buds, and our results indicate that the isolated taste buds were intact and functional approaches for examining the taste senses of the chicken using Ca²⁺ imaging can be informative.  相似文献   

Distribution and seasonal activity variation of proteases in digestive tract of sea cucumber Stichopus japonicus     

Xueyan Fu  Changhu Xue  Benchun Miao  Zhaojie Li  Xin Gao  Takashi Hirata 《Fisheries Science》2006,72(5):1130-1132

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Morphological characteristics of Nigorobuna <Emphasis Type="Italic">Carassius auratus grandoculis</Emphasis> called Io in Lake Nishino     

Takashi?SuzukiEmail author  Hajime?Nagano  Toru?Kobayashi  Koichi?Ueno 《Fisheries Science》2005,71(3):679-681

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Comparison of environmental conditions in two representative oyster farming areas: Hiroshima Bay,western Japan and Oginoham a Bay (a branch of Ishinomaki Bay), northern Japan   总被引：1，自引：0，他引：1  

Takashi?KamiyamaEmail author  Hiroyuki?Yamauchi  Takuro?Iwai  Shoichi?Hanawa  Yukihiko?Matsuyama  Satoshi?Arima  Yuichi?Kotani 《Fisheries Science》2005,71(6):1295-1303

ABSTRACT:   Sea water environmental conditions over annual cycles were investigated and compared between two oyster farming areas, western Hiroshima Bay and Oginohama Bay (a branch of Ishinomaki Bay) in Miyagi Prefecture, to appropriately manage oyster culture or more efficiently utilize farming areas. The environmental parameters of temperature, salinity, nutrient concentrations (NO₂–N, NO₃–N, NH₄–N, PO₄–P, and SiO₂–Si) and size-fractionated chlorophyll- a (<0.2, 2–20, >20µm), and abundances of microzooplankton were measured in each bay at the surface, and 2 and 5 m depth layers. Differences in the annual mean values and results with monthly paired Student's t -tests showed that salinity was lower, and temperature, nutrient (especially PO₄–P) and chlorophyll- a concentrations, and abundance of microzooplankton, were higher in Hiroshima Bay than in Oginohama Bay. Differences in environmental conditions between inshore and offshore areas of each bay suggest that inflows of river water in western Hiroshima Bay and sea water from offshore had the most significant effects on the environmental conditions. It is concluded that such oceanographic and biological differences strongly affect the oyster farming system, especially regarding the optimum usage of offshore areas in Summer under clean, cold and stable seawater conditions, rather than food quantity in Hiroshima Bay, and under more abundant food conditions in Oginohama Bay.  相似文献   

Refined calculation model for NaLA, a fishing net shape simulator, applicable to gill nets   总被引：1，自引：0，他引：1  

Takashi  SHIMIZU  Tsutomu  TAKAGI  Katsuya  SUZUKI  Tomonori  HIRAISHI  Katsutaro  YAMAMOTO 《Fisheries Science》2004,70(3):401-411

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Diving behavior of immature, feeding Pacific bluefin tuna (Thunnus thynnus orientalis) in relation to season and area: the East China Sea and the Kuroshio–Oyashio transition region     

Takashi Kitagawa  Shingo Kimura  Hideaki Nakata  Harumi Yamada 《Fisheries Oceanography》2004,13(3):161-180

Twenty‐four archival tags were recovered from Pacific bluefin tuna previously released off Tsushima Island in the East China Sea. By analysis of the time‐series data of the pressure and the ambient and internal temperature from the 24 tags, we examined the relationship between the tuna's pattern of diving and the thermocline depth. In the East China Sea, diving and feeding events occurred throughout almost the entire day in both winter and summer, suggesting that the purpose of diving is for feeding. In summer, the feeding frequency was greater than that in winter, which corresponds to the fact that growth is more rapid in summer than in winter. During summer in the Kuroshio–Oyashio transition region, on the other hand, feeding events were much more frequent than those in the East China Sea, in spite of a lower diving frequency. The mean horizontal distance traveled was also significantly higher and it seems that in this area they may move horizontally to feed on prey accumulated at the surface. We conclude that, in addition to the ambient temperature structure, the vertical and horizontal distribution of prey species plays an important role in the feeding behavior of Pacific bluefin tuna. One bluefin tuna migrated to the Oyashio frontal area, where both the horizontal and the vertical thermal gradients are much steeper. The fish spent most of the time on the warmer side of the front and often traveled horizontally to the colder side during the day, perhaps to feed. This implies that there is a thermal barrier effect, in this case from the Oyashio front, on their behavior. The frequency of feeding events was low, although all the monitored fish dived every dawn and dusk, irrespective of the seasons or location. It is possible that these twice‐daily diving patterns occurred in response to the change in ambient light at sunrise and sunset.  相似文献