Effect of fusion/activation protocol on in vitro development of porcine nuclear transfer embryos constructed with foreign gene-transfected fetal fibroblasts     

Martinez Diaz MA  Mori T  Nagano M  Katagiri S  Takahashi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):989-994

The effect of fusion/activation protocol on in vitro development of porcine nuclear transfer (NT) embryos constructed with foreign gene-transfected somatic cells were investigated. NT embryos were produced by using enucleated M II oocytes and enhanced green fluorescence protein (EGFP) gene-transfected or non-transfected porcine fetal fibroblasts. One group of NT embryos received a single electrical pulse to induce fusion and activation simultaneously (FAS). The other group was fused 2 hr before activation (FBA) using two kinds of electrical pulses. Electrically activated NT embryos in both groups were treated with cycloheximide (CHX) before culture to assess the development to the blastocyst stage. After 6 days of culture, all morulae and blastocysts derived from EGFP-transfected fibroblasts emitted green fluorescence without mosaicism, and EGFP-gene product was also detected in all morulae and blastocysts examined. NT embryos undergoing FAS showed higher developmental capacity to blastocysts than those undergoing FBA, regardless of the EGFP transfection into the nuclear donor cells. The results also indicated that EGFP-gene transfection into nuclear donor cells has no obvious deleterious effect on the development of NT embryos to blastocysts.  相似文献   

Efficiency of fecal steroid hormone measurement for assessing reproductive function in the Hokkaido brown bear (Ursus arctos yesoensis)     

Ishikawa A  Kikuchi S  Katagiri S  Sakamoto H  Takahashi Y 《The Japanese journal of veterinary research》2002,50(1):17-27

The present study aimed to establish simple systems for measuring fecal steroid hormones in order to monitor the reproductive profiles of captive Hokkaido brown bears. The efficiency of fecal sample processing at the steps of dehydration and extraction and the correlation between steroid concentrations in matched fecal and blood samples were studied. Then, monthly changes in fecal estradiol-17 beta and progesterone in female bears, and testosterone in male bears were examined. The procedure was finalized as follows. Fecal samples were dried at 100 degrees C for 3 hr and extracted with diethyl ether. The diethyl ether in the extracts was evaporated and residues were reconstituted in ethanol for the assays. Hormone concentrations were quantified using enzyme immunoassays. Concentrations of progesterone and testosterone in fecal and plasma samples were correlated in the systems. The changes in fecal progesterone and testosterone concentrations were similar to those in serum concentrations of bears as reported previously. In contrast, fecal estradiol concentrations did not correlate with plasma levels probably because of the time lag in excretion. However, the changes in estradiol-17 beta concentrations in feces in the present study were similar to those reported in serum. In conclusion, fecal progesterone and testosterone assay systems appear practical for monitoring ovarian and testicular activities without immobilization, though methodological improvements and further validation may be required. For the fecal estradiol-17 beta assay, there is a need to solve the problem of excretion time lag before the system can be used in the study of reproductive physiology.  相似文献   

1Alpha-hydroxyvitamin D3 prevents the decrease of bone mineral density in lactating beagles     

Ichikawa F  Katagiri K  Higuchi Y  Takeda S  Saito K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(1):75-79

We assessed the change of bone mineral density (BMD) in lactating beagles with dual energy X-ray absorptiometry (DXA) and the preventive effect of 1alpha-hydroxyvitamin D3 (1alpha(OH)D3) on the BMD. Beagles, two to five years old, were used for detecting the time course change of BMD. Since the coefficient of variation (CV(%)) on detecting lumber vertebral (L2-L4) and tibial BMD by DXA was about 0.5%, DXA was useful to detect the change of BMD in beagles. There was a marked decrease in vertebral BMD during lactational period in the control group. The BMD levels after weaning were found to reverse to the initial level at mating. The same tendency was observed in tibial BMD as vertebral BMD, though the BMD changes were not marked. Beagles were administered at a dose of 0.1 microg/kg of 1alpha(OH)D3 three times in a week, and it was found to suppress the decrease in vertebral BMD during the breast feeding period. Also, the administration of 1alpha(OH)D3 promoted the prevention of decreased BMD during lactation both in vertebrae and tibiae. Significant effects of 1alpha(OH)D3 administration on tibial BMD were not observed. No adverse effects, such as hypercalcemia and hypercalciuria, were observed during the experimental period. Therefore, DXA was useful for detecting the changes of BMD in lactating beagles and the change of BMD was marked in lumber vertebrae, which are rich in trabecular bone. The preventive effect of 1alpha(OH)D3 on the decrease of BMD during the lactation period was observed in beagles.  相似文献   

Granulocyte colony-stimulating factor has no adverse effects on atherosclerotic lesions in high cholesterol-fed miniature Swine     

Takai H  Miyoshi A  Yamazaki M  Adachi K  Katagiri K  Arakawa H  Katsuyama K  Ito T  Fujii E  Hayashi S  Kato A  Suzuki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(9):943-950

Granulocyte colony-stimulating factor (G-CSF) is widely used to mobilize peripheral blood stem cells, and expected to restore cardiac function for patients with coronary artery diseases as a consequence of progression of atherosclerosis. Safety issues related to the administration of G-CSF to these patients, however, are still under study. The animal model for atherosclerosis was produced by feeding miniature swine a high-cholesterol diet for 3 months. G-CSF (5 or 10 microg/kg/day) was given to the animal model by daily subcutaneous injections for 10 days and 20 main arteries were evaluated pathologically. In addition, the general toxicological effects were studied on clinical signs, body weight, hematology, blood chemistry and pathology. In the G-CSF-treated groups, a variety of changes related to the major pharmacological activity of G-CSF including an increase in white blood cell (WBC) counts were observed. In many arteries, atherosclerotic lesions similar to Type I-V of the proposed classification by the American Heart Association were observed. No effects of the G-CSF treatment were seen on the histopathological findings, incidence, severity or distribution of atherosclerotic lesions. In addition, no infiltration of neutrophils to the lesions was observed. These findings suggest that the administration of G-CSF causes neither exacerbation or modification of atherosclerotic lesions nor adverse changes despite that a sufficient increase in WBC counts could be achieved in the peripheral blood.  相似文献   

A progestin-based treatment with a high dose of estradiol benzoate normalizes cyclic changes in endometrial EGF concentrations and restores fertility in repeat breeder cows     

Katagiri S  Takahashi Y 《The Journal of reproduction and development》2008,54(6):473-479

The objective of the present study was to examine the efficacy of a progestin-based treatment with a high dose of estradiol benzoate (EB) to normalize the epidermal growth factor (EGF) profile in the uterine endometrium and restore fertility in repeat breeder cows. Repeat breeder cows without peaks in their endometrial EGF concentrations on Days 3 and 14 of the estrous cycle were used throughout the study. The effect of 1 (standard dose), 2.5 and 5 mg of EB in a progestin-based treatment protocol (EB1, EB2.5 and EB5 treatments, respectively; n=5 for each group) on endometrial EGF concentrations was first examined. The EB1 and EB2.5 treatments in the repeat breeder cows produced a suppressed response in endometrial EGF compared with EB1 treatment in the fertile controls (n=5) and failed to restore the normal EGF profile during the next estrous cycle. However, EB5 treatment produced an increase in EGF concentrations similar to the fertile controls and normalized the endometrial EGF profile. The effects of the EB1 and EB5 treatments (n=30 for each group) on the endometrial EGF profile and fertility were then examined in the repeat breeder cows. The proportion of cows, with an EGF profile normalized by the treatments was higher in the EB5 group (66.7%) than in the EB1 (30.0%) and untreated control (13.3%; n=30) groups (P<0.01). The pregnancy rates of the cows having a normal EGF profile after treatment in the EB1 and EB5 groups were similar (88.9 and 85.0%, respectively) and higher than those of the cows having an abnormal profile within the same groups (19.0 and 30.0%, respectively, P<0.01). In summary, the endometrial response to EB in terms of the EGF concentration was suppressed in repeat breeder cows. A high dose (5 mg) of EB in a progestin-based treatment was found to be effective for restoration of a normal EGF profile and fertility in repeat breeder cows having lesser endometrial EGF concentrations on Days 3 and 14.  相似文献   

Detection of Anaplasma bovis and Anaplasma phagocytophilum from cattle on Yonaguni Island, Okinawa, Japan     

Ooshiro M  Zakimi S  Matsukawa Y  Katagiri Y  Inokuma H 《Veterinary parasitology》2008,154(3-4):360-364

DNA fragments of Anaplasma bovis and Anaplasma phagocytophilum were detected in cattle on Yonaguni Island, Okinawa, Japan. Although the pathogenesis and epidemiology of these pathogens have not yet been clarified, this is the first detection of their presence in cattle from Japan.  相似文献   

The relationship between oocyte morphology and ovarian status in cattle     

Nagano M  Hishinuma M  Katagiri S  Takahashi Y 《The Journal of reproduction and development》2007,53(4):953-958

We investigated the relationships between oocyte morphology, follicular size and follicular waves using bovine ovaries derived from local abattoirs. Ovaries at the recruitment and selection phases contained larger numbers of oocytes with good developmental ability, although ovaries at the recruitment phase contained the largest numbers of follicles compared with ovaries at the selection and dominant phases. Dominant phase ovaries contained a high percentage of oocytes with as good developmental ability as selection phase ovaries; however, they contained the lowest total number of oocytes with good developmental ability. Small follicles under 3.0 mm in diameter contained large numbers of small and degenerating oocytes. In contrast, follicles more than 3.0 mm in diameter contained a higher percentage of oocytes with good developmental ability.  相似文献   

荒漠化土地上粗砂和草根对风蚀的影响     

YuYi  OkumuraTakenobu 《中国水土保持》2002,(7):43-44

我们进行了一系列风洞实验来阐明有成行排列根的沙地表面砂化过程的特征及其对侵蚀率的影响。实验在一个横截面为 50ｃｍ× 4 0ｃｍ ,长 1 7ｍ的室内风洞 (顺翼展方向的 )进行。准备了 6种砂样品 ,并放置在 6 0ｃｍ× 30ｃｍ的砂盘上。每种样品由细砂 (ｄ <0 .84ｍｍ)和粗砂 (  相似文献   

Oxygenation: a specific soluble cytochrome p-450 coupled enzyme complex     

Gunsalus IC  Ganguli BN  Katagiri M  Tsibris JC  Debrunner P  Frauenfelder H 《Science (New York, N.Y.)》1968,160(3826):438-439

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Neuronal pathway from the liver modulates energy expenditure and systemic insulin sensitivity     

Uno K  Katagiri H  Yamada T  Ishigaki Y  Ogihara T  Imai J  Hasegawa Y  Gao J  Kaneko K  Iwasaki H  Ishihara H  Sasano H  Inukai K  Mizuguchi H  Asano T  Shiota M  Nakazato M  Oka Y 《Science (New York, N.Y.)》2006,312(5780):1656-1659

Coordinated control of energy metabolism and glucose homeostasis requires communication between organs and tissues. We identified a neuronal pathway that participates in the cross talk between the liver and adipose tissue. By studying a mouse model, we showed that adenovirus-mediated expression of peroxisome proliferator-activated receptor (PPAR)-g2 in the liver induces acute hepatic steatosis while markedly decreasing peripheral adiposity. These changes were accompanied by increased energy expenditure and improved systemic insulin sensitivity. Hepatic vagotomy and selective afferent blockage of the hepatic vagus revealed that the effects on peripheral tissues involve the afferent vagal nerve. Furthermore, an antidiabetic thiazolidinedione, a PPARg agonist, enhanced this pathway. This neuronal pathway from the liver may function to protect against metabolic perturbation induced by excessive energy storage.  相似文献