Effects of phosphate supply and elevated CO2 on root acid phosphatase activity in Pinus densiflora seedlings     

Mariko Norisada  Tetsuya Motoshige  Katsumi Kojima  Takeshi Tange 《植物养料与土壤学杂志》2006,169(2):274-279

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Enterotoxigenicity of heat-resistant and heat-sensitive strains of Clostridium perfringens     

H Sunagawa  T Tsuzuki  K Takeshi  Y Ando  S Oka 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(5):853-858

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Liver lesions and serum antibodies in mice surviving Tyzzer's disease     

H Nakayama  S Inoue  A Nii  A Yasuda  N Okada  K Fujiwara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(1):189-192

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Pathologic observations of mouse liver infected with Tyzzer's organisms using immunoperoxidase method   总被引：2，自引：0，他引：2  

N Okada  W Toriumi  K Sakamoto  K Fujiwara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(1):89-98

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An equine case of granular cell tumor with chondroplasia     

S Inoue  N Okada  K Midoro  H Nakayama  R Takahashi  K Fujiwara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(3):581-583

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Flow cytometric assay in peripheral blood of dogs--reference values for leukocytes from Brazilian beagles     

Fujiwara RT  Bueno LL  Vale AM  Franca-Silva JC  da Costa RT  Quetz Jda S  Machado-Coelho GL  Reis AB  Martins Filho OA  Genaro O  Nascimento E  Mayrink W 《Polish journal of veterinary sciences》2005,8(1):17-22

Use of domestic reference values in the flow cytometry analysis is known to improve its accuracy by integrating local variations as gender, race and age. Up to date application of flow cytometry in veterinary medicine has been limited to describe the percentual values just for peripheral lymphocytes subsets of blood. We now report establishment of reference values for a wide range of proportional and absolute numbers of peripheral blood leukocytes, including T cells subsets, B cells, monocytes and eosinophils, applicable to the healthy population of Beagles in Brazil and other regions with similar demographic characteristics. Normal reference values were also established to estimate the gender-related differences. This information will provide clinical aid in the evaluation of immunologic status as well as standard values for experimental animals of dogs from Brazil and other similar regions.  相似文献   

Nucleocapsid-like structures of Ebola virus reconstructed using electron tomography     

Noda T  Aoyama K  Sagara H  Kida H  Kawaoka Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(3):325-328

Electron tomography (ET) is a new technique for high resolution, three-dimensional (3D) reconstruction of pleiomorphic macromolecular complexes, such as virus components. By employing this technique, we resolved the 3D structure of Ebola virus nucleocapsid-like (NC-like) structures in the cytoplasm of cells expressing NP, VP24, and VP35: the minimum components required to form these NC-like structures. Reconstruction of these tubular NC-like structures of Ebola virus showed them to be composed of left-handed helices spaced at short intervals, which is structurally consistent with other non-segmented negative-strand RNA viruses.  相似文献   

The effect of pretreatment with different doses of GnRH to synchronize follicular wave on superstimulation of follicular growth in dairy cattle   总被引：1，自引：0，他引：1  

Sato T  Nakada K  Uchiyama Y  Kimura Y  Fujiwara N  Sato Y  Umeda M  Furukawa T 《The Journal of reproduction and development》2005,51(5):573-578

The objective of this study was to evaluate the efficiency of gonadotropin releasing hormone (GnRH) and GnRH doses in synchronizing follicular wave emergence as a pretreatment for superovulation in cattle. Fourteen Holstein-Friesian cows 6 days from estrus were randomly assigned to receive 100 microg (n=4), 50 microg (n=5), or 25 microg (n=5) of GnRH. Superovulation was induced with injections of porcine FSH (pFSH) twice daily, decreasing the dose (total 42 AU) over 5 days beginning 2.5 days after receiving GnRH. On the 7th and 8th injections of pFSH, 750 microg of PGF(2alpha) was also given. With the exception of one cow that was given 50 microg of GnRH, ovulation was induced in all cows from the three groups and the new follicular wave emergence was observed. The total number of follicles for the 25 microg GnRH group was less than that observed for the 100 microg GnRH group (P<0.05), although there were no differences between the 100 microg, 50 microg and 25 microg GnRH groups with respect to the number of preovulatory follicles (>or=10 mm) and CL. The numbers of normal embryos were greater for the 25 microg GnRH group than the 100 or 50 microg GnRH groups (P<0.01); however, the numbers of ova/embryos did not differ significantly between the three groups. These results suggest that 25 microg of GnRH was sufficient to induce ovulation and follicular wave emergence. On day 6 of the estrous cycle, a reduction of the dose of GnRH to synchronize follicular wave emergence as a pretreatment for superstimulation promotes transferable embryos.  相似文献   

Participation of Rhipicephalus sanguineus (Acari: Ixodidae) in the epidemiology of canine visceral leishmaniasis     

Coutinho MT  Bueno LL  Sterzik A  Fujiwara RT  Botelho JR  De Maria M  Genaro O  Linardi PM 《Veterinary parasitology》2005,128(1-2):149-155

The vectorial competence of the tick Rhipicephalus sanguineus is discussed in relation to the epidemiology of canine visceral leishmaniasis, taking into account its strict association with dogs and the low indices of natural infection presented by its known vector, the phlebotomine sand fly Lutzomyia longipalpis. In order to evaluate natural infection by Leishmania chagasi and the infectivity of these parasites in the tick, 39 specimens (6 females, 11 males and 22 nymphs) of R. sanguineus were removed from 21 dogs showing diverse symptoms of zoonotic visceral leishmaniasis (ZVL). Six ticks (15.4%) gave positive results for the genus Leishmania using the PCR technique. To determine the infectivity of the parasites, 36 hamsters were inoculated orally and peritoneally with macerates of ticks removed from nine dogs symptomatic for visceral leishmaniasis. After 6 months the hamsters were sacrificed and necropsied. Serum was removed for IFAT, as well as spleen and liver fragments to make imprint smears and for PCR. Eight (88.9%) of these dogs presented ticks that were infective for 14 hamsters (41.2%), 12 (85.7%) of them infected peritoneally and two (14.3%) orally. PCR revealed 27 smears (40.9%) to be positive, 20 (62.5%) of them infected peritoneally and seven (20.6%) orally. IFAT showed 14 positive animals (41.2%). Based on these findings, we suggest that the vectorial capacity of R. sanguineus for L. chagasi should be evaluated further, opening new perspectives in the epidemiology of ZVL.  相似文献   

Canine interstitial nephritis with special reference to glomerular lesions and filariasis.     

K Shirota  R Takahashi  K Fujiwara  A Hasegawa 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1979,41(2):119-129

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