Effect of ovary lipid from Skipjack tuna (Katsuwonus pelamis) on brain monoamines in rats     

Ookawa K  Mochizuki K  Shida E  Suzuki T  Suzuki T  Ooba T  Matumoto T  Hokari Y  Yokogoshi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):593-598

In our previous experiments with rats, ovary lipid from Skipjack Tuna (Katsuwonus pelamis) (OLS) was shown to have a mitigating effect on anxiety and/or fear in elevated T-maze tests. This suggests that OLS has some effect on the central nervous system (CNS) of rats. Thus, we performed experiments to examine the status of CNS in rats given OLS. The effect of OLS on chronic stress was also examined at the same time. The feed for control rats used oil and fat that have the same energy percentages for n-6 and n-3 fatty acids and the same n-6/n-3 ratio as OLS. As a result, rats given OLS for 28 days had lower serotonin levels in various brain areas regardless of stress application, showing that OLS affected the serotonin nervous system. From this, it was inferred that the ability of OLS to mitigate anxiety and/or fear resulted from its action on CNS, especially the serotonin nervous system. Substances other than the essential fatty acids may have been responsible for the action of OLS on monoamines and the metabolites. The effect of OLS on CNS, especially the serotonin nervous system, suggests that OLS may suppress anxiety.  相似文献   

Oleosome-Associated Protein of the Oleaginous Diatom Fistulifera solaris Contains an Endoplasmic Reticulum-Targeting Signal Sequence     

Yoshiaki Maeda  Yoshihiko Sunaga  Tomoko Yoshino  Tsuyoshi Tanaka 《Marine drugs》2014,12(7):3892-3903

Microalgae tend to accumulate lipids as an energy storage material in the specific organelle, oleosomes. Current studies have demonstrated that lipids derived from microalgal oleosomes are a promising source of biofuels, while the oleosome formation mechanism has not been fully elucidated. Oleosome-associated proteins have been identified from several microalgae to elucidate the fundamental mechanisms of oleosome formation, although understanding their functions is still in infancy. Recently, we discovered a diatom-oleosome-associated-protein 1 (DOAP1) from the oleaginous diatom, Fistulifera solaris JPCC DA0580. The DOAP1 sequence implied that this protein might be transported into the endoplasmic reticulum (ER) due to the signal sequence. To ensure this, we fused the signal sequence to green fluorescence protein. The fusion protein distributed around the chloroplast as like a meshwork membrane structure, indicating the ER localization. This result suggests that DOAP1 could firstly localize at the ER, then move to the oleosomes. This study also demonstrated that the DOAP1 signal sequence allowed recombinant proteins to be specifically expressed in the ER of the oleaginous diatom. It would be a useful technique for engineering the lipid synthesis pathways existing in the ER, and finally controlling the biofuel quality.  相似文献   

Absorption of color additives and settling volume in water of blue-green alga,ishikurage (Nostoc commune)     

Kohji Hori  Tomoko Ueno-Mohri  Takuo Okita 《Plant foods for human nutrition (Dordrecht, Netherlands)》1992,42(1):31-36

The properties of ishikurage (Nostoc commune) and other algae were compared. Ishikurage and suizenji nori (Aphanothece sacrum) absorbed much more amaranth than others. They have a high settling volume (SV) in water. The high SV of ishikurage is attributable to its neutral detergent fiber (NDF). The acid detergent fiber (ADF) from the alga had a high amaranth absorption. However the absorption by the ADF did not explain the absorption by ishikurage.The results suggest that ishikurage may have a nutritional significance as a source of dietary fiber.  相似文献   

Abundance of sulphate-reducing bacteria in fish farm sediments along the coast of Japan and South Korea     

Ryuji Kondo  Kotaro Shigematsu  Naoki Kawahara  Takahiko Okamura  Yang Ho Yoon  Tomoko Sakami  Hisashi Yokoyama  Yoshitsugu Koizumi 《Fisheries Science》2012,78(1):123-131

The abundance of sulphate-reducing bacteria (SRB) was investigated in the coastal marine sediments along the coast of Japan and South Korea. Sediment samples were collected from fish and shellfish farms between 2006 and 2008. As non-fish farming reference sites, sediments were also collected from highly eutrophic bays, a highly sulphidogenic saline lake, and the deep sea. A quantitative real-time PCR analysis that targeted the gene coding for a portion of the α-subunit of dissimilatory sulphite reductase (dsrA) was performed to assess the abundance of the SRB in the sediments. Between 2.8 × 10⁷ and 2.5 × 10⁹ copies of the dsrA gene per gram dry sediment were detected. There was no relationship between dsrA gene copy number and total bacterial count in the sediments, whereas organic matter contents (particulate organic carbon and nitrogen, ignition loss and chemical oxygen demand) and acid-volatile sulphide contents were significantly correlated with the dsrA copy number. The data presented demonstrate that organic enrichment of sediment may influence the abundance of SRB communities in coastal marine sediments and that the cell density of SRB may be used as a biological indicator for assessing pollution levels in sediments of marine fish farms.  相似文献   

Promotion of root elongation and ion uptake in rice seedlings by 4,4,4-trifluoro-3-(indole-3-)butyric acid   总被引：2，自引：0，他引：2  

Xiuping LI  Tomoko SUZUKI  Hideo SASAKAWA 《Soil Science and Plant Nutrition》2009,55(3):385-393

  相似文献   

The detection of endogenous 2’-deoxymugineic acid in olives (Olea europaea L.) indicates the biosynthesis of mugineic acid family phytosiderophores in non-graminaceous plants     

Motofumi Suzuki  Tomoko Nozoye  Seiji Nagasaka  Hiromi Nakanishi  Naoko K. Nishizawa  Satoshi Mori 《Soil Science and Plant Nutrition》2016,62(5-6):481-488

  相似文献   

Construction of a linkage map and identification of DNA markers linked to Fom-1, a gene conferring resistance to Fusarium oxysporum f.sp. melonis race 2 in melon     

Takahiro Tezuka  Keisuke Waki  Kazutoshi Yashiro  Maki Kuzuya  Tomoko Ishikawa  Yasumasa Takatsu  Makoto Miyagi 《Euphytica》2009,168(2):177-188

Resistance to Fusarium oxysporum f.sp. melonis race 2 is conferred by a single dominant gene, Fom-1 in melon. Here, we identified DNA markers tightly linked to Fom-1 that could be used for marker assisted selection in breeding programs. First, we developed 125 F₂ plants derived from the cross between melon lines P11 (fom-1fom-1) and MR-1 (Fom-1Fom-1). Using the F₂ population, we constructed a linkage map including 14 SSR markers which had not been mapped previously. Fom-1 was confirmed to be allocated to linkage group 7. Then, we identified four AFLP markers using bulked segregant analysis. The AFLP marker TAG/GCC-470 was completely linked to Fom-1 and other three markers were mapped near Fom-1. TAG/GCC-470 and TCG/GGT-400 were respectively converted to STS and CAPS markers. Usefulness of DNA markers was confirmed in the analysis with several melon cultivars and lines.  相似文献   

Determination of the rate of 18F-labeled water movement to the leaf and its association with water relations in acid soil-tolerant rice varieties     

Dong-Jin Kang  Tomoko M. Nakanishi  Tamikazu Kume  Ryuichi Ishii 《Journal of Crop Science and Biotechnology》2009,12(4):261-265

The visual symptoms of damage by acid sulfate soil look quite similar to those due to soil moisture deficit. The present paper is to confirm if acid soil-tolerance is associated with drought tolerance in terms of leaf water potential, transpiration, and dry matter production. Seedlings of seven acid soil-tolerant (acid-tolerant), and three acid soil-intolerant varieties (acid-intolerant) were subjected to low pH conditions (pH 3.8) for 48 hours. The rate of water uptake was determined continuously by measuring radioactivity in the collar part (target area) of rice leaves exposed to ¹⁸F-labeled water (¹⁸F-water) using a Positron Emitting Tracer Imaging System (PETIS). The PETIS measurement showed that the radioactivity in the target position of leaves of acid-tolerant varieties increased faster than that of the intolerant varieties after the ¹⁸F-water was applied into the vial. In the plants subjected to low pH conditions, the transpiration rate (Tr) and leaf water potential (Ψ) decreased in both acid-tolerant and intolerant varieties. However, the extent of reduction was less in the acid-tolerant varieties than in the intolerant ones. Moreover, the dry matter production rate of the acid-tolerant varieties was significantly higher than that of the acid-intolerant ones in the low pH conditions. This study clearly shows that ¹⁸F-water uptake in a leaf was higher, and water relations were also better maintained in the acid-tolerant varieties compared with intolerant ones, resulting in higher growth rate in the acid-tolerant varieties, when plants were exposed to the acid solution conditions. We conclude that acid soil-tolerance is closely associated with leaf water relations in rice plants.  相似文献   

Antifungal activity of <Emphasis Type="Italic">Biscogniauxia</Emphasis> sp. culture filtrates against the rice pathogen <Emphasis Type="Italic">Magnaporthe oryzae</Emphasis>     

Quyet Thi Nguyen  Kana Ueda  Tomoko Tamura  Junichi Kihara  Makoto Ueno 《Journal of General Plant Pathology》2017,83(2):109-112

An ethyl acetate extract of a culture filtrate (ECF) from an unidentified fungal isolate O821 was evaluated for antifungal activity against the rice pathogen Magnaporthe oryzae. The O821-ECF significantly inhibited spore germination, appressorium formation, and mycelial growth of M. oryzae, and its antifungal activity was heat-stable. It also significantly suppressed the number and size of blast lesions. In an analysis of the ITS sequence of this isolate, it shared similarities with species of the fungus Biscogniauxia. These results suggest that isolate O821 of the genus Biscogniauxia produces a heat-stable antifungal compound(s) in its culture filtrate.  相似文献   

New localities and awn morphology variation in Miscanthus ×ogiformis          下载免费PDF全文

Hiroshi Hayakawa  Aya Nishiwaki  Tomoko Nishida  Akihiro Konuma 《Weed Biology and Management》2017,17(3):136-143

Miscanthus ×ogiformis is a hybrid between Miscanthus sacchariflorus and Miscanthus sinensis and its several synonyms indicate the insufficient understanding of the morphological variation of its awn on the spikelet, which is a key characteristic for identifying Miscanthus species. In the present study, all the Miscanthus specimens in 19 herbaria were examined in order to find new localities for Miscanthus ×ogiformis. Thirty‐nine Miscanthus ×ogiformis specimens appeared to be naturally occurring hybrids, as their callus hair length, awn length, and callus‐to‐spikelet ratio differed from those found in the parental species. Therefore, new Miscanthus ×ogiformis individuals with morphological variations are likely to be discovered and these strains might be useful as new genetic resources for this biomass crop.  相似文献