A note on the effect of temperature on true and apparent metabolisable energy values of a layer diet     

M. Yamazaki  Zhang Zi‐Yi 《British poultry science》1982,23(5):447-450

The effect of ambient temperature on the true (TME) and apparent metabolisable energy (AME) values of a layer diet was examined using White Leghorn cockerels.

The TME value found at the cool temperature (5 to 15 °C) was significantly higher than those found at hot (25 to 35 °G) and control (22 °C) temperatures. AME values were not significantly affected.  相似文献   

Canine CD20 gene     

Kano R  Inoiue C  Okano H  Yamazaki J  Takahashi T  Watari T  Tokuriki M  Hasegawa A 《Veterinary immunology and immunopathology》2005,108(3-4):265-268

The human CD20 antigen, a 35kDa cell surface nonglycosylated hydrophobic phoshpoprotein is expressed consistently on almost all human B-cells, and its monoclonal antibody is used for the therapy on human B-cell lymphoma. In the present study, canine CD20 gene was cloned and sequenced, and the expression of CD20 mRNA was investigated in canine peripheral blood mononuclear cells (PBMCs), and lymph nodes from healthy dogs, and canine lymphoma cells. Using canine cDNA as a template, full-length of canine CD20 gene was sequenced by 5'-RACE and 3'-RACE methods. The full-length of the cDNA sequence of canine CD20 was 1239bp encoding 297 amino acids. The amino acid sequences of canine CD20 showed 73 and 68% sequence similarities with those of human and mouse, respectively. Canine CD20 was predicted to contain domains of amino acid sequences consisting of two extracellular domains (EM), four transmembrane domains (TM), and three intracellular domains (IC) as in human CD20. Canine CD20 mRNA was detected in PBMCs and lymph node from healthy dogs, and B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells by RT-PCR analysis. From these results, canine CD20 might be targeted for monoclonal antibody therapy against B-cell lymphoma of dogs.  相似文献   

Expression of apoptosis-related gene mRNAs in feline T-cells infected with feline immunodeficiency virus (FIV)     

Yamazaki J  Hasebe N  Nagafuchi S  Baba K  Tsujimoto H  Kano R  Hasegawa A 《Veterinary microbiology》2004,101(1):1-8

In the present study, full length of feline bax, bcl-2, bcl-xL and caspase 3 genes were sequenced and the expression of these mRNAs were also investigated in FIV-infected lymphocytes. The full length cDNA sequence of bax (646 bp), bcl-2 (1423 bp), bcl-xL (1163 bp) and caspase 3 genes (1208 bp) contained a single open reading frame of 579 bp coding 193 amino acids, 708 bp coding 236 amino acids, 702 bp coding 234 amino acids and 834 bp coding 278 amino acids, respectively. Number of apoptotic Kumi-1 cells gradually increased after FIV infection and approximately 70% were apoptotic and 30% were viable in the cells infected with FIV after 8-day incubation, though approximately 80% were non-apoptotic and 20% were dead in non-infected cells. The expression of bcl-2 mRNA in lymphocytes of established cell line was increased by FIV. The amounts of mRNAs of bax, caspase 3 and bcl-xL in FIV-infected cells were not different from those in uninfected control cells.  相似文献   

Ultrastructure and C-type particles in myeloid leukemia of a pig     

K Kadota  M Yamazaki  S Ishino  T Nei 《Veterinary pathology》1984,21(2):263-265

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Formation of detached tail and coiled tail of sperm in a beagle dog     

Kawakami E  Ozawa T  Hirano T  Hori T  Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(1):83-85

The right testis and epididymis were excised from a Beagle dog that ejaculated high percentages of sperm with detached tails and with coiled tails. Cross sections of the organs were stamped on glass slides and histological examination of the organs was performed to find the portion where sperm with the abnormal tails appear. Many sperm with tails whose axoneme was exposed near the neck region were observed in the testis and they decreased in order from the caput, to the corpus, and the cauda epididymis. Sperm with detached tails and sperm with coiled tails gradually increased in the epididymis. These findings indicate that the tails of sperm with an exposed axoneme detached in the epididymis.  相似文献   

Carcinogenicity of biphenyl in mice by two years feeding     

Umeda Y  Aiso S  Yamazaki K  Ohnishi M  Arito H  Nagano K  Yamamoto S  Matsushima T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(4):417-424

Carcinogenicity and chronic toxicity of biphenyl was examined in the male and female BDF1 mice fed a diet containing biphenyl at 667, 2,000 or 6,000 ppm for 2 years. There was no difference in survival rate between any biphenyl-containing diet-fed group of either sex and the respective control. Body weights of the males and females fed 6,000 ppm diet were significantly lower than the respective control. Incidences of hepatocellular carcinomas and hepatocellular adenomas in the females fed diets containing biphenyl were significantly increased in a dose-related manner, and exceeded a range of the historical control data in the Japan Bioassay Research Center. Incidences of basophilic cell foci in the liver were increased in the females fed 2,000 and 6,000 ppm diets. There was no increase in tumor or tumor-related lesion in the males fed diets containing biphenyl. Chronic toxicity of biphenyl was characterized by increased incidences of urothelial desquamation in the renal pelvis in males and females and mineralization in the inner stripe of renal outer medulla in females, as well as changes in serum levels of BUN, ALP and some electrolytes in males and females. In conclusion, the 2-year oral administration of biphenyl-containing diets induced pre-neoplastic and neoplastic lesions in the liver of females and non-neoplastic lesions in the kidney of males and females. Causative factors for the biphenyl-induced hepatocarcinogenicity were discussed in light of our published finding of peroxisome proliferation.  相似文献   

Effect of antineoplastic drugs on the expression of Bcl-2 and Bcl-xL genes in the feline T-cell leukemia cell line     

Sano J  Nagafuchi S  Yamazaki J  Oguma K  Kano R  Hasegawa A 《Research in veterinary science》2005,79(3):197-201

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Comparison of fattening performance in Brahman grade cattle (Bos indicus) and crossbred water buffalo (Bubalus bubalis) fed on high roughage diet     

Rosalina M. LAPITAN  Arnel N. DEL BARRIO  Osamu KATSUBE  Tomomi BAN-TOKUDA  Edgar A. ORDEN  Alberto Y. ROBLES  Libertado C. CRUZ  Yukio KANAI  Tsutomu FUJIHARA 《Animal Science Journal》2008,79(1):76-82

The objective of this study was to compare the fattening performance in Brahman grade cattle (crossbred cattle) and crossbred water buffalo at the same young age and fed with high roughage based fattening rations in the Philippines. Ten crossbred cattle and 10 crossbred water buffalo, aged between 18 and 24 months old were used in this experiment. The animals were fed diets consisting of 85% Napier or Para grass and 15% concentrate mixture (CM) on a dry matter (DM) basis. The grass, total DM intake and bodyweight gain were significantly (P < 0.01) higher for the crossbred water buffalo than for the cattle. There was no species significant difference in the digestion coefficient and feed conversion rate between the crossbred cattle and water buffalo. The return over feed cost for fattening was significantly (P < 0.05) higher in the crossbred water buffalo than in the cattle. These results clearly indicate that under high roughage‐based fattening rations, young crossbred water buffalo are better able to utilize the roughage and they perform better in terms of feed intake and live weight gains than the crossbred cattle in the Philippines.  相似文献   

Antibiotic furazolidone induces CYP1A but not CYP2E1 subfamily in rat liver     

Sasaki N  Sakai N  Ikenaka Y  Kamiya T  Heewon M  Sakamoto KQ  Ishizuka M  Fujita S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(3):223-226

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Plasma steroid hormone concentrations and their relationships in Suffolk ewes during gestation and parturition     

Hiroshi Miura  Tatsuya Yamazaki  Motohiro Kikuchi  Minoru Sakaguchi 《Animal Science Journal》2019,90(11):1426-1431

In this study, we measured plasma concentrations of progesterone, pregnenolone, estradiol, estrone, estrone sulfate, and cortisol and analyzed the correlations between these hormones during gestation in 13 Suffolk ewes, the main breed in Japan. Progesterone increased during gestation and decreased a few days before parturition; however, this pattern was different in samples with high progesterone concentrations (P4 spike samples). This P4 spike was associated with a high pregnenolone concentration. Apart from the P4 spike, the progesterone change was similar to that in other sheep breeds. Pregnenolone increased during gestation and decreased after parturition. A significant correlation between progesterone and pregnenolone was observed a few days before parturition. Estrone sulfate and estradiol concentrations increased during gestation, but estrone did not. They increased shortly before parturition, and then decreased immediately after parturition. At parturition, the correlation between estrone and estrone sulfate was significantly stronger. Moreover, a strong correlation between estrone sulfate and estradiol was observed after parturition. Cortisol did not change during gestation and increased shortly before parturition. The results showed steroid hormone dynamics in normal pregnant Suffolk ewes, which were mostly in line with those of other sheep breeds. It should be noted that high progesterone concentrations altered the typical patterns.  相似文献