农田耕作与土壤盐分的变化     

雷玉平  田魁祥  孙景玉  山崎素直  但野利秋 《中国生态农业学报》1995,3(3):71-74

采用原状土隔离池进行了盐分运移的定位试验。结果表明：含盐量0.11-0.28%的轻盐渍化或脱盐潮土处在不断脱盐过程中，平均年脱盐速率为0.0125%；小麦种植期，土壤处于返盐过程，盐分在上层积累；夏玉米种植期，土壤处于脱盐过程；盐分运动是一个盐离子的层次间传递过程，不同盐离子传递的强度不同，以氯根和纳离子变动最大，而硫酸根和钙离子变幅较小，碳酸根较稳定。  相似文献   

Glycoprotein derived from the hot water extract of mint plant, Perilla frutescens britton   总被引：1，自引：0，他引：1  

Asada M  Fukumori Y  Inoue M  Nakagomi K  Sugie M  Fujita Y  Tomizuka N  Yamazaki Y  Oka S 《Journal of agricultural and food chemistry》1999,47(2):468-472

Glycoprotein showing inhibitory activity against mast cell degranulation and hyaluronidase activity was purified from the hot water extract of mint plant (Perilla frutescens Britton). The purified inhibitor gave a single band detected with Coomassie brilliant blue staining and periodic acid-Schiff staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The molecular mass was estimated to be 6.0 kDa on SDS-PAGE. The inhibitor did not become inactivated when boiled for 30 min or digested with trypsin, V8 protease, or proteinase K but was inactivated by NaIO(4) oxidation. The inhibitor prevented mast cell degranulation and hyaluronidase activity (IC(50) = 0.42 mg/mL) in a dose-dependent manner. The inhibitor also inhibited the protein kinase C activity. It is possible to purify and characterize a glycoprotein with putative pharmacological properties from mint plants.  相似文献   

Structural identification of new curcumin dimers and their contribution to the antioxidant mechanism of curcumin     

Masuda T  Toi Y  Bando H  Maekawa T  Takeda Y  Yamaguchi H 《Journal of agricultural and food chemistry》2002,50(9):2524-2530

As a part of the research project on the elucidation of the chain-breaking antioxidant mechanism of natural phenolics against the oxidation of food components, curcumin, a main turmeric pigment, was investigated. A relatively high concentration of curcumin gave three dimers as radical termination products in addition to the coupling products with curcumin and the lipid hydroperoxide. The structural analysis of these dimers and quantitative analysis of their production rates revealed that radical-radical termination mainly occurred at the 2-position of curcumin. The contribution of the pathway for production of these dimers to the antioxidant mechanism of curcumin was estimated from the concentration-dependent data of the antioxidant activity and formation rates of these termination products. The A-A termination (dimer formation) was estimated to contribute at least about 40% of the entire antioxidant process against ethyl linoleate oxidation.  相似文献   

Assessment of biomarkers influencing treatment success on small intestinal lymphoma in dogs     

Hiroki Yamazaki  Hiroshi Sasai  Miyuu Tanaka  Toshiyuki Tanaka  Hidetaka Nishida  Shunsuke Noguchi  Mitsuru Kuwamura  Hideo Akiyoshi 《Veterinary and comparative oncology》2021,19(1):123-131

This study aimed to determine a reliable therapeutic biomarker for localized small intestinal lymphoma (SIL) in dogs based on clinical and histopathological features. We retrospectively investigated 84 dogs with localized SIL, including 36 dogs receiving surgery and 48 dogs receiving chemotherapy. The dogs receiving surgery were divided into two subgroups: 18 dogs (group 1) with overall survival (OS) <120 days (median OS) and 18 dogs (group 2) with OS ≥120 days. Correspondingly, the dogs receiving chemotherapy were divided into 24 dogs (group 3) with OS <98 days (median OS) and 24 dogs (group 4) with OS ≥98 days. Clinical, haematological, histopathological and immunohistochemical analyses were comparatively evaluated among the four subgroups. There was no significant difference in OS between the surgery and chemotherapy groups. In dogs receiving surgery, the rate of Ki67‐positive cells was significantly increased in group 1 compared to group 2 and showed no significant difference between groups 3 and 4. In dogs receiving chemotherapy, the rate of O6‐methylguanine‐DNA methyltransferase (MGMT) was significantly higher in group 3 than in group 4 and showed no significant difference between groups 1 and 2. Additionally, our data showed that OS in dogs with higher Ki67 expression might be significantly increased by chemotherapy than by surgery, that of those with higher MGMT expression might be significantly increased by surgery than by chemotherapy, and Ki67 and MGMT were independent of each other. Indices of Ki67 and MGMT are suggested therapeutic biomarkers to determine the optimal first‐line treatment for localized SIL in dogs.  相似文献   

Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection     

Tomomi TAKANO  Haruna WATANABE  Tomoyoshi DOKI  Hajime KUSUHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):805

Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs.  相似文献   

Overexpression of xyloglucanase (AaXEG2) accelerates heteroblastic development in mangium leaves     

Sri Hartati  Enny Sudarmonowati  Tomomi Kaku  Hisato Ikegaya  Rumi Kaida  Kei’ichi Baba  Takahisa Hayashi 《Journal of Wood Science》2011,57(6):463-469

Transgenic mangium trees (Acacia mangium) overexpressing xyloglucanase (AaXEG2) were generated by spraying flower buds with Agrobacterium solution and allowing seeds to develop. The overexpression of xyloglucanase decreased xyloglucan content in the cell walls and increased stem length and diameter. The leaves of the transgenic seedlings exhibited accelerated heteroblastic development, proceeding from the stage of three bipinnate leaves to that of enlarging petiole 2 weeks earlier than wild type seedlings did.  相似文献   

Recognition of H-2 types in relation to the blocking of pregnancy in mice   总被引：12，自引：0，他引：12  

K Yamazaki  G K Beauchamp  C J Wysocki  J Bard  L Thomas  E A Boyse 《Science (New York, N.Y.)》1983,221(4606):186-188

Inbred BALB/c females were mated and subsequently exposed in a divided cage to "stimulus" males or females whose H-2 type was similar or dissimilar to the stud male's. The incidence of pregnancy blocking was considerably higher when stud and stimulus males differed in H-2 type than when they did not. Similar results were obtained with urine samples of H-2 identical and nonidentical males. Females exposed after mating to other females whose H-2 type differed from the stud male, under the same experimental conditions, also showed an appreciable incidence of pregnancy block. It is therefore concluded that chemosensory recognition of H-2 types affects the reproductive hormonal status of the pregnant female.  相似文献   

Differential antigen processing by dendritic cell subsets in vivo   总被引：2，自引：0，他引：2  

Dudziak D  Kamphorst AO  Heidkamp GF  Buchholz VR  Trumpfheller C  Yamazaki S  Cheong C  Liu K  Lee HW  Park CG  Steinman RM  Nussenzweig MC 《Science (New York, N.Y.)》2007,315(5808):107-111

Dendritic cells (DCs) process and present self and foreign antigens to induce tolerance or immunity. In vitro models suggest that induction of immunity is controlled by regulating the presentation of antigen, but little is known about how DCs control antigen presentation in vivo. To examine antigen processing and presentation in vivo, we specifically targeted antigens to two major subsets of DCs by using chimeric monoclonal antibodies. Unlike CD8+ DCs that express the cell surface protein CD205, CD8- DCs, which are positive for the 33D1 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II. This difference in antigen processing is intrinsic to the DC subsets and is associated with increased expression of proteins involved in MHC processing.  相似文献   

Familial imprinting determines H-2 selective mating preferences   总被引：15，自引：0，他引：15  

K Yamazaki  G K Beauchamp  D Kupniewski  J Bard  L Thomas  E A Boyse 《Science (New York, N.Y.)》1988,240(4857):1331-1332

Inbred male mice typically prefer to mate with females of a different, non-self H-2 haplotype. To determine whether this natural preference is irrevocable or results from familial imprinting, a test system was used which relied on previous observations that B6 males (H-2b) mate preferentially with congenic B6-H-2k rather than B6 females, and B6-H-2k males with B6 females. This preference was reversed in B6 males fostered by B6-H-2k parents and in B6-H-2k males fostered by B6 parents, preference in these cases favoring the same H-2 type. Thus, H-2 selective mating preference is acquired by imprinting on familial H-2 types.  相似文献   

The effect of magmatic activity on hydrothermal venting along the superfast-spreading East pacific rise     

Urabe T  Baker ET  Ishibashi J  Feely RA  Marumo K  Massoth GJ  Maruyama A  Shitashima K  Okamura K  Lupton JE  Sonoda A  Yamazaki T  Aoki M  Gendron J  Greene R  Kaiho Y  Kisimoto K  Lebon G  Matsumoto T  Nakamura K  Nishizawa A  Okano O  Paradis G  Roe K  Shibata T  Tennant D  Vance T  Walker SL  Yabuki T  Ytow N 《Science (New York, N.Y.)》1995,269(5227):1092-1095

A survey of hydrothermal activity along the superfast-spreading (approximately 150 millimeters per year) East Pacific Rise shows that hydrothermal plumes overlay approximately 60 percent of the ridge crest between 13 degrees 50' and 18 degrees 40'S, a plume abundance nearly twice that known from any other rige portion of comparable length. Plumes were most abundant where the axial cross section is inflated and an axial magma chamber is present. Plumes with high ratios of volatile ((3)He, CH(4), and H(2)S) to nonvolatile (Mn and Fe) species marked where hydrothermal circulation has been perturbed by recent magmatic activity. The high proportion of volatile-rich plumes observed implies that such episodes are more frequent here than on slower spreading ridges.  相似文献