Washout Ratio in the Hepatic Vein Measured by Contrast‐Enhanced Ultrasonography to Distinguish Between Inflammatory and Noninflammatory Hepatic Disorders in Dogs          下载免费PDF全文

K. Morishita  A. Hiramoto  A. Michishita  S. Takagi  T. Osuga  S.Y. Lim  K. Nakamura  N. Sasaki  H. Ohta  M. Takiguchi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2017,31(3):770-777

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Controlling factors for the distribution of sapric peat soils extracted by GIS analysis     

Mizuki Morishita 《Soil Science and Plant Nutrition》2019,65(1):65-72

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Serum resistance as an indicator of virulence of Pasteurella multocida for turkeys     

T Y Morishita  K P Snipes  T E Carpenter 《Avian diseases》1990,34(4):888-892

Wildlife isolates of Pasteurella multocida, whose virulence for turkeys had previously been determined by intravenous inoculation, were characterized regarding their ability to survive incubation in fresh non-immune turkey serum. The relative virulence of the isolates was significantly associated with their ability to resist the bactericidal power of the serum as determined by standard plate counts following incubation. Organisms with a high survival value were more virulent; those with a low survival value were less virulent. A statistical model was specified and was successfully used to predict relative virulence of the P. multocida isolates. This method of assaying serum resistance was rapid, repeatable, and practical and could be performed with minimal laboratory equipment. Also studied was the serum resistance of seven serotype 3, 4 isolates obtained from the lungs of M9-vaccinated turkeys from seven flocks experiencing increased mortality due to fowl cholera. These isolates were shown to be identical to the M9 vaccine by restriction endonuclease analysis of chromosomal DNA. Six of the seven isolates had higher serum survival values than the original M9 vaccine.  相似文献   

Expression of bovine cytokines in Escherichia coli   总被引：7，自引：0，他引：7  

Kashima T  Morishita A  Iwata H  Maeda K  Inoue T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(2):171-173

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Four-legged broiler chicken with two cloacae and three ceca     

Ebako GM  Morishita TY  Mattoon JS 《Avian diseases》2002,46(1):234-238

This case report examines an Arbor Acres female x Peterson male crossbred 6-wk-old female broiler chicken with four legs, two cloacae, and three ceca. The intestines occupied the caudodorsal portion of the abdominal cavity with three ceca attached to the terminal end of the ileum. The left lateral cecum was larger and had a divided distal end that terminated into 2-cm-long blind sacs. The rectum was dilated and divided into two cloacae that each opened on the dorsolateral aspect of both the right and left cloacal elevator muscles. The two extra legs were attached to the pygostyle by cartilage and skin tissue. Both femoral heads of the extraneous legs were fused together. The extra legs were smaller in size (approximately 17 cm in length) compared with the normal legs (approximately 22 cm). The femoral heads of the two normal legs were disarticulated from the acetabulum.  相似文献   

Evaluation of Pasteurella multocida isolated from rabbits by capsular typing, somatic serotyping, and restriction endonuclease analysis.     

Amna B El Tayeb  Teresa Y Morishita  Elisabeth J Angrick 《Journal of veterinary diagnostic investigation》2004,16(2):121-125

The goal of this study was to characterize Pasteurella multocida isolated from rabbits. Five hundred and fifty-three apparently healthy rabbits were sampled for this study. Nasal swabs were collected from each rabbit for P. multocida isolation and identification. Isolates were further characterized by capsular and somatic antigens and genomic DNA fingerprinting. Thirty-nine P. multocida isolates were recovered from 553 rabbits (7%). Capsular typing was done by depolymerization of P. multocida capsule by Staphylococcus aureus hyaluronidase and by disc diffusion with mucopolysaccharidase enzymes (heparinase III, chondroitinase AC, and hyaluronidase). Thirty-one (79%) of the isolates were capsular type A, and 8 isolates (21%) had untypable (UT) capsules. The gel-diffusion precipitin test was used to determine the somatic type of P. multocida isolates. Nineteen isolates were somatic serotype 3 (49%), 12 were serotype 1 (31%), 1 was serotype 2, 2 were serotype 5, 2 were serotype 12 with a weak reaction to antiserum raised against serotype 7 (5%), and 1 was serotype 4. Two of the isolates (5%) were UT. Restriction endonuclease analysis of the DNA of the isolates revealed 7 distinct profiles by digestion with HindIII, and 12 profiles were obtained with HpaII, whereas digestion with EcoRI did not differentiate between any of the P. multocida DNA isolates studied. The DNA restriction endonuclease enzyme HpaII was found more useful for differentiating between DNA fingerprints of P. multocida rabbit isolates. However, no correlation between capsular type, somatic serotypes, and DNA fingerprints was seen in this study.  相似文献   

Somaclonal variation in Tricyrtis hirta plants regenerated from 1-year-old embryogenic callus cultures     

Masaru Nakano  Toshikazu Nomizu  Keiko Mizunashi  Mami Suzuki  Shiro Mori  Sachiko Kuwayama  Megumi Hayashi  Hiroto Umehara  Eriko Oka  Hiroyuki Kobayashi  Megumi Asano  Shintaro Sugawara  Hiroki Takagi  Hiroyuki Saito  Masashi Nakata  Toshinari Godo  Yoshihiro Hara  Junji Amano 《Scientia Horticulturae》2006,110(4):366-371

The Liliaceous perennial Tricyrtis hirta, sometimes called ‘Japanese toad lily’, has recently become popular as an ornamental for pot and garden uses. Highly embryogenic callus cultures of this plant predominately consisted of diploid cells but also contained tetraploid cells after 1 year of establishment. In the present study, plans regenerated from the 1-year-old embryogenic callus cultures were subjected to ploidy level analysis and morphological characterization following 3 years of cultivation. Among 37 plants examined, 28 kept the diploid level (2n = 2x = 26) but nine were tetraploid (2n = 4x = 52) as indicated by FCM analysis and chromosome observation. Although no morphological alterations were detected in 26 out of 28 diploid regenerants, the remaining two showed noticeable variations: both were severely dwarf and had crimped leaves and many malformed flowers. The tetraploid regenerants had several horticulturally attractive characteristics compared with the diploid controls, such as longer shoots, thicker stems, and larger flowers. Thus regeneration of tetraploid plants from 1-year-old embryogenic callus cultures offers a possibility to improve the horticultural value of T. hirta, although regeneration of trueness-to-type plants is essential for utilizing the cultures for micropropagation and genetic transformation.  相似文献   

Genetic diversity ofKandelia candel andBruguiera gymnorrhiza in the Southwest Islands, Japan     

Toshikazu Takeuchi  Takashi Sugaya  Ayako Kanazashi  Hiroshi Yoshimaru  Masaki Katsuta 《Journal of Forest Research》2001,6(3):157-162

The genetic diversity of the mangrove tree speciesKandelia candel andBruguiera gymnorrhiza in the Southwest Islands of Japan was investigated. These islands are located at the periphery of the distribution area of the two species, and their populations are relatively small. Allozyme analyses of 17 loci inK. candel and 13 loci inB. gynmorrhiza revealed very little genetic variation in both species. At the species level, the proportion of polymorphic loci was 4.2%, the average effective number of alleles per locus was 1.02, and the average expected heterozygosity was 0.012 inK. candel. The corresponding figures forB. gymnorrhiza were 9.0%, 1.06 and 0.035, respectively. However, the coefficients of genetic differentiation among the populations were high (G _ST=0.165 forK. candel and 0.253 forB. gymnorrhiza). The genetic variation and habitat area ofK. candel on Amami Island is greater than on the islands Okinawa and Iriomote. The genetic variation and habitat area ofB. gymnorrhiza is greater on Iriomote Island than on the islands Okinawa and Amami. The level of genetic variation in both of the species might be related to their population sizes.  相似文献   

Sensitivity of FCV to recombinant feline interferon (rFeIFN)   总被引：1，自引：0，他引：1  

Ohe K  Takahashi T  Hara D  Hara M 《Veterinary research communications》2008,32(2):167-174

Feline calicivirus cause feline respiratory diseases, and inactivated and attenuated vaccines are available for its prevention. Moreover, the presence of vaccine breakdown strains (VBS) is problematic. In Japan, feline recombinant interferon (rFeIFN) has been used for its treatment. However the method of compare with each strains has not established. To examine the relationship between the breakdown vaccine strain and rFeIFN sensitivity, the sensitivity of 47 field isolates to rFeIFN was determined. The Log PDD₅₀ values were normally distributed within the range 1.1–3.7, with a mean value of 2.3 ± 0.64. Since 68.3% of the PDD values fell in the range of the mean ± standard deviation, the values in the range 1.7–2.9, the lower values, and the higher values were defined as representing moderate, low, and high sensitivity, respectively. Among the 15 vaccine breakdown strains, strain Fukuoka9 showed a low sensitivity, but strains ML89, T58, and N74 were highly sensitive, showing no association with vaccine breakdown. The amino acid sequence changes specific to the low rFeIFN-sensitive Fukuoka-9 strain were found, suggesting that these sites are involved in rFeIFN sensitivity.  相似文献   

Usefulness of noninvasive shear wave elastography for the assessment of hepatic fibrosis in dogs with hepatic disease     

Masahiro Tamura  Hiroshi Ohta  Genya Shimbo  Tatsuyuki Osuga  Noboru Sasaki  Keitaro Morishita  Yumiko Kagawa  Mitsuyoshi Takiguchi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(5):2067-2074

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