Transcranial Doppler Ultrasound Examination in Dogs with Suspected Intracranial Hypertension Caused by Neurologic Diseases          下载免费PDF全文

K. Sasaoka  K. Nakamura  T. Osuga  T. Morita  N. Yokoyama  K. Morishita  N. Sasaki  H. Ohta  M. Takiguchi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):314-323

Background

Transcranial Doppler ultrasound examination (TCD) is a rapid, noninvasive technique used to evaluate cerebral blood flow and is useful for the detection of intracranial hypertension in humans. However, the clinical usefulness of TCD in diagnosing intracranial hypertension has not been demonstrated for intracranial diseases in dogs.

Objectives

To determine the association between the TCD variables and intracranial hypertension in dogs with intracranial diseases.

Animals

Fifty client‐owned dogs with neurologic signs.

Methods

Cross‐sectional study. All dogs underwent TCD of the basilar artery under isoflurane anesthesia after magnetic resonance imaging (MRI). Dogs were classified into 3 groups based on MRI findings: no structural diseases (group I), structural disease without MRI evidence of intracranial hypertension (group II), and structural disease with MRI evidence of intracranial hypertension (group III). The TCD vascular resistance variables (resistive index [RI], pulsatility index [PI], and the ratio of systolic to diastolic mean velocity [Sm/Dm]) were measured.

Results

Fifteen, 22, and 13 dogs were classified into groups I, II, and III, respectively. Dogs in group III had significantly higher Sm/Dm (median, 1.78; range, 1.44–2.58) than those in group I (median, 1.63; range, 1.43–1.75) and group II (median, 1.62; range, 1.27–2.10). No significant differences in RI and PI were identified among groups.

Conclusions and Clinical Importance

Our findings suggest that increased Sm/Dm is associated with MRI findings of suspected intracranial hypertension in dogs with intracranial diseases and that TCD could be a useful tool to help to diagnose intracranial hypertension.  相似文献   

Contrast‐enhanced ultrasonography is a feasible technique for quantifying hepatic microvascular perfusion in dogs with extrahepatic congenital portosystemic shunts     

Masahiro Tamura  Hiroshi Ohta  Khoirun Nisa  Tatsuyuki Osuga  Noboru Sasaki  Keitaro Morishita  Mitsuyoshi Takiguchi 《Veterinary radiology & ultrasound》2019,60(2):192-200

Extrahepatic‐congenital portosystemic shunt is a vascular anomaly that connects the portal vein to the systemic circulation and leads to a change in hepatic microvascular perfusion. However, an assessment of hepatic microvascular perfusion is limited by conventional diagnostic modalities. The aim of this prospective, exploratory study was to assess hepatic microvascular perfusion in dogs with extrahepatic‐congenital portosystemic shunt using contrast‐enhanced ultrasonography (CEUS) using perfluorobutane (Sonazoid^®). A total of 17 dogs were included, eight healthy dogs and nine with extrahepatic‐congenital portosystemic shunt. The time‐to‐peak (TTP), rising time (RT), and rising rate (RR) in the hepatic artery, portal vein, and hepatic parenchyma, as well as the portal vein‐to‐hepatic parenchyma transit time (ΔHP‐PV) measured from time‐intensity curve on CEUS were compared between healthy and extrahepatic‐congenital portosystemic shunt dogs. The RT of the hepatic artery in extrahepatic‐congenital portosystemic shunt dogs was significantly earlier than in healthy dogs (P = 0.0153). The TTP and RT of the hepatic parenchyma were significantly earlier in extrahepatic‐congenital portosystemic shunt dogs than in healthy dogs (P = 0.0018 and P = 0.0024, respectively). ΔHP–PV was significantly shorter in extrahepatic‐congenital portosystemic shunt dogs than in healthy dogs (P = 0.0018). CEUS effectively revealed changes in hepatic microvascular perfusion including hepatic artery, portal vein, and hepatic parenchyma simultaneously in extrahepatic‐congenital portosystemic shunt dogs. Rapid hepatic artery and hepatic parenchyma enhancements may reflect a compensatory increase in hepatic artery blood flow (arterialization) caused by a decrease in portal vein blood flow and may be used as an additional diagnostic test to distinguish extrahepatic‐congenital portosystemic shunt dogs from healthy dogs.  相似文献   

Prognostic Value of Right Ventricular Tei Index in Dogs with Myxomatous Mitral Valvular Heart Disease          下载免费PDF全文

K. Nakamura  T. Morita  T. Osuga  K. Morishita  N. Sasaki  H. Ohta  M. Takiguchi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(1):69-75

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Spatial distribution analysis of the North Pacific spiny dogfish,Squalus suckleyi,in the North Pacific using generalized additive models          下载免费PDF全文

Toshikazu Yano  Seiji Ohshimo  Minoru Kanaiwa  Tsutomu Hattori  Masa‐aki Fukuwaka  Toru Nagasawa  Sho Tanaka 《Fisheries Oceanography》2017,26(6):668-679

The North Pacific spiny dogfish (SPD), Squalus suckleyi, is a commercially exploited shark species that plays an important role in the ecosystem. To elucidate the distribution of the SPD in the North Pacific and to evaluate the effects of sea surface temperature (SST) and prey availability on its distribution, we estimated the probability of SPD presence using a generalized additive model with a binomial error distribution from SPD presence/absence data on 14,824 operations in fishery‐independent gillnet surveys between 1972 and 2011. The habitat model was structured in the east and west to reflect differences in the North Pacific oceanic environments. In the east, a higher probability of SPD presence was identified along the coast from the eastern Gulf of Alaska to Queen Charlotte Sound. In the west, it was identified around northern Japan. The estimated distribution was continuous between the two areas, whereas the probability of SPD presence was relatively low. Although the probability of SPD presence was higher at SSTs between 6°C and 12°C, the SST at the peak probability of SPD presence differed between the west and east. The prey species, Japanese sardine, Sardinops melanostictus, and walleye pollock, Gadus chalcogrammus, in the west and boreal clubhook squid, Onychoteuthis borealijaponica, in the east significantly affected the probability of SPD presence, which was higher if the prey species co‐existed with SPD. Therefore, SPD might adapt their distribution to that of available prey species. SPD stock assessment and management in these two important areas are required for its sustainable utilization.  相似文献   

Food-entrainable circadian oscillator in goldfish: multiple daily feeding times and food-anticipatory activity     

Sunuma  Toshikazu  Amano  Masafumi  Iigo  Masayuki  Yamamori  Kunio 《Fisheries Science》2009,75(1):207-214

The existence of a food-entrainable circadian oscillator (FEO) was examined in goldfish Carassius auratus. Single goldfish were exposed to light–dark (LD) 12:12 (lights on 06:00–18:00 h) and fed at 12:00 h (phase I). The photoperiod regime was shifted to constant light (LL) (phase II). The fish were then fed at 06:00 or at 18:00 h (phase III), and all fish were fasted (phase IV). In both groups, three out of eight fish exhibited food-anticipatory activity (FAA) in phases II and III, the free-running period (τ) of locomotor activity was approximately 24.0 h, and circadian rhythms of locomotor activity started to free-run from the previous FAA in phase IV, indicating the existence of an FEO. Next, single goldfish were exposed to LD 12:12 and were fed twice daily at 12:00 and 00:00 h (phase I). The photoperiod regime was shifted to LL (phase II), and the fish were fasted (phase III). In phase II, FAA was observed in three out of six fish for both feeding times. In phase III, a circadian rhythm of locomotor activity was observed in five fish, and circadian rhythms started to free-run from the previous feeding time (either 12:00 or 00:00 h) in three fish. These results suggest that individual goldfish can remember two daily feeding times.  相似文献   

Isolation and sequence analysis of the promoter and an allelic sequence of the iron-sulfur protein subunit gene from the white-rot fungusPleurotus ostreatus     

Toshikazu Irie  Yoichi Honda  Takuro Matsuyama  Takashi Watanabe  Masaaki Kuwahara 《Journal of Wood Science》1998,44(6):491-494

We have isolated a structural gene ofsdil, which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase (EC 1.3.99.1), from a white-rot basidiomycete,Pleurotus ostreatus. Here we report isolation of the promoter region ofsdil and an allelic sequence encoding the second-type cDNA fragment isolated in the former experiments. The nucleotide sequence analysis of the promoter region revealed the existence of putative CAAT and TATA boxes, which permits us to develop an expression system in this species. The Southern blot analysis and the restriction fragment length polymorphism assay using monokaryotic strains demonstrated that no family genes tosdil exist in the haploid genome ofP. ostreatus. Moreover, a genetic analysis to detect a linkage between thesdil genotypes and flutolanil resistance in the mutantP. ostreatus strains was also developed.  相似文献   

Spatial and seasonal variations of radiocesium concentrations in an algae-grazing annual fish,ayu Plecoglossus altivelis collected from Fukushima Prefecture in 2014     

Morishita  Daigo  Wada  Toshihiro  Noda  Takuji  Tomiya  Atsushi  Enomoto  Masahiro  Sato  Toshiyuki  Suzuki  Shunji  Kawata  Gyo 《Fisheries Science》2019,85(3):561-569

Fisheries Science - To elucidate spatial and seasonal variations of radiocesium (134Cs and 137Cs) concentrations in ayu Plecoglossus altivelis, amphidromous fish samples were collected both from...  相似文献   

Correction to: Spatial and seasonal variations of radiocesium concentrations in an algae-grazing annual fish,ayu Plecoglossus altivelis collected from Fukushima Prefecture in 2014     

Morishita  Daigo  Wada  Toshihiro  Noda  Takuji  Tomiya  Atsushi  Enomoto  Masahiro  Sato  Toshiyuki  Suzuki  Shunji  Kawata  Gyo 《Fisheries Science》2019,85(3):571-571

Fisheries Science - In the original publication the caption of Fig. 6a and 6b is incorrectly published.  相似文献   

Action mechanism of an angiotensin I-converting enzyme inhibitory peptide derived from chicken breast muscle     

Saiga A  Okumura T  Makihara T  Katsuda S  Morimatsu F  Nishimura T 《Journal of agricultural and food chemistry》2006,54(3):942-945

In a previous study, we isolated the inhibitory peptide (P4 peptide, Gly-Phe-Hyp-Gly-Thr-Hyp-Gly-Leu-Hyp-Gly-Phe) for angiotensin I-converting enzyme (ACE) from chicken breast muscle extract possessing hypotensive activity for spontaneously hypertensive rats (SHRs). This study was performed to elucidate the peptide's action mechanisms of inhibiting ACE. Intravenous administration of synthetic P4 peptide resulted in significant drops in the blood pressures of SHRs. As Dixon plots indicate, the P4 peptide showed high affinity toward ACE (K(i) = 11.48 microM) and only 10% of the total amount of the P4 peptide was decomposed. The analyses of the relationship between the ACE inhibitory activity and structure of the P4 peptide clarified that Hyp-Gly-Leu-Hyp-Gly-Phe showed a stronger activity (IC50 = 10 microM) than the P4 peptide (IC50 = 46 microM). When Phe at the C-terminus of the P4 peptide was deleted, IC50 changed to 25000 microM, indicating that Phe at the C-terminus of the peptide is very important for ACE inhibitory activity.  相似文献   

CONTRAST‐ENHANCED ULTRASONOGRAPHIC FINDINGS IN THREE DOGS WITH PANCREATIC INSULINOMA          下载免费PDF全文

Kensuke Nakamura  Sue‐Yee Lim  Kenji Ochiai  Masahiro Yamasaki  Hiroshi Ohta  Keitaro Morishita  Satoshi Takagi  Mitsuyoshi Takiguchi 《Veterinary radiology & ultrasound》2015,56(1):55-62

Abdominal ultrasonography is one of the most common diagnostic imaging modalities used for dogs with suspected insulinoma; however, pancreatic masses are clearly identified in fewer than half of affected dogs and benign pancreatic nodules can be difficult to differentiate from malignant ones. The purpose of this prospective study was to describe contrast‐enhanced ultrasonography (CEUS) characteristics of confirmed pancreatic insulinoma in a group of dogs. Inclusion criteria were as follows: (1) repeated hypoglycemia (blood glucose levels <60 mg/dl, twice or more); (2) elevated blood insulin levels with hypoglycemia; (3) pancreatic nodules detected with conventional ultrasonography; and (4) histological confirmation of pancreatic islet cell carcinoma. Immediately following conventional ultrasonography of the entire abdomen, CEUS of the pancreatic nodule and adjacent parenchyma was performed using contrast‐specific technology pulse inversion imaging and perflubutane microbubble contrast agent. Three dogs met inclusion criteria. Pancreatic nodules in all the three dogs became more clearly demarcated after injection of the contrast agent. Each nodule showed different enhancement patterns: markedly hyperechoic for 5 s, slightly hyperechoic for 1 s, and clearly hypoechoic for over 30 s. These results were not in complete agreement with previously reported CEUS findings in human patients with insulinoma. All nodules were surgically resected and histopathologically confirmed as malignant insulinomas. Findings from the current study indicated that contrast‐enhanced ultrasound may help to increase conspicuity of pancreatic insulinomas in dogs and that enhancement characteristics may be more variable in dogs than in humans.  相似文献