Epidemiological, bacteriological and molecular studies on caseous lymphadenitis in Sirohi goats of Rajasthan, India     

Kumar J  Singh F  Tripathi BN  Kumar R  Dixit SK  Sonawane GG 《Tropical animal health and production》2012,44(7):1319-1322

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CL), a chronic debilitating disease of goats. In the present study, a total of 575 goats of Sirohi breed on an organized farm situated in the semi-arid tropical region of Rajasthan, India were clinically examined. Pus samples from superficial lymph nodes of 27 (4.7%) adult goats presenting clinical lesions suggestive of CL were collected for bacteriological and molecular analyses. Of these goats, 51.9% yielded C. pseudotuberculosis on the basis of morphological, cultural and biochemical characteristics. A polymerase chain reaction (PCR) assay targeting proline iminopeptidase gene specific to C. pseudotuberculosis was developed that confirmed all 14 bacterial isolates. The specificity of the PCR product was confirmed by sequencing of the 551-bp amplicon in both senses, showing 98-100% homology with published sequences. Thus, overall prevalence rate based on clinical, bacterial culture and PCR assay were found to be 4.7%, 2.4% and 2.4%, respectively. The PCR assay developed in this study was found to be specific and rapid, and could be used for confirmation of CL in goats as an alternative method to generally cumbersome, time-consuming and less reliable conventional methods.  相似文献   

Estimating carbon sequestration potential of existing agroforestry systems in India     

Ajit  S. K. Dhyani  A. K. Handa  Ram Newaj  S. B. Chavan  Badre Alam  Rajendra Prasad  Asha Ram  R. H. Rizvi  Amit Kumar Jain  Uma  Dharmendra Tripathi  R. R. Shakhela  A. G. Patel  V. V. Dalvi  A. K. Saxena  A. K. S. Parihar  M. R. Backiyavathy  R. J. Sudhagar  C. Bandeswaran  S. Gunasekaran 《Agroforestry Systems》2017,91(6):1101-1118

India launched National Agroforestry Policy on 10th February, 2014 which has the potential to substantially reduce poverty in rural India and revive wood based industry, besides integrating food production with environmental services. The policy is not only crucial to India’s ambitious goal of achieving 33 per cent forest and tree cover but also to mitigate GHG emissions from agriculture sector. Dynamic CO2FIX-v3.1 model has been used to estimate the carbon sequestration potential (CSP) of existing agroforestry systems (AFS) for simulation period of 30 years in twenty six districts from ten selected states of India. The observed number of trees on farmers’ field in these districts varied from 1.81 to 204 per hectare with an average value of 19.44 trees per hectare. The biomass in the tree component varied from 0.58 to 48.50 Mg DM ha^?1, whereas, the total biomass (tree and crop) ranged from 4.96 to 58.96 Mg DM ha^?1. The soil organic carbon ranged from 4.28 to 24.13 Mg C ha^?1. The average estimated carbon sequestration potential of the AFS, representing varying edapho-climatic conditions, on farmers field at country level was 0.21 Mg C ha^?1yr^?1. At national level, existing AFS are estimated to mitigate 109.34 million tons CO₂ annually, which may offsets one-third (33 %) of the total GHG emissions from agriculture sector.  相似文献   

Nitrate leaching,nitrous oxide emission and N use efficiency of aerobic rice under different N application strategy     

Sangita Mohanty  C. K. Swain  Rahul Tripathi  S. K. Sethi  P. Bhattacharyya  Anjani Kumar 《Archives of Agronomy and Soil Science》2018,64(4):465-479

A field study was conducted in the sub-humid tropical region of India to examine the effect of different nitrogen (N) management strategies on nitrate leaching, nitrous oxide (N₂O) emission and N use efficiency in aerobic rice. Treatments were: control (no N), 120 kg N ha^?1 applied as prilled urea (PU) in conventional method, 120 kg N ha^?1 applied as neem coated urea (NCU) in conventional method, N applied as PU on the basis of leaf colour chart (LCC) reading, N applied as NCU on the basis of LCC reading, and 120 kg N ha^?1 applied as PU and farm yard manure (FYM) in 1:1 ratio. Results showed that 3.4–16.1 kg NO₃-N ha^?1 was leached below 45 cm depth and 0.61–1.12 kg N₂O-N ha^?1 was emitted from aerobic rice during the growing season. NCU when applied conventionally reduced nitrate-nitrogen (NO₃-N) leaching and N₂O emission by 18.6% and 21.4%, respectively However when applied on the basis of LCC reading NCU reduced NO₃-N leaching by 39.8% as compared to PU applied in conventional method. NCU when applied on the basis of LCC reading synchronized N supply with demand and reduced N loss, which resulted in higher yield and N use efficiency.  相似文献   

First natural co-occurrence of tomato leaf curl New Delhi virus DNA-A and chili leaf curl betasatellite on tomato plants (<Emphasis Type="Italic">Solanum lycopersicum</Emphasis> L.) in India     

Aniruddha Kumar Agnihotri  Sudhakar Prasad Mishra  Ramesh Chandra Tripathi  Mohammad Ansar  Ashish Srivastava  Indra Prasad Tripathi 《Journal of General Plant Pathology》2018,84(6):414-417

Tomato leaf curl disease (ToLCD) affected 25% of the tomato crop in Chitrakoot, India and symptomatic leaves were collected for molecular assay. The complete sequences of bipartite begomovirus DNA-A and a betasatellite DNA were amplified. In a sequence analysis, begomovirus DNA-A and betasatellite shared highest sequence identity (91–99%) with Tomato leaf curl New Delhi virus (ToLCNDV) DNA-A and chili leaf curl betasatellite (ChLCB), respectively. The virus was transmitted by whitefly to tomato plants and caused ToLCD symptoms with 70% transmission rate. To our knowledge, this is the first report of the natural occurrence of ToLCNDV and ChLCB in India.  相似文献   

Phytoremediation of Mercury and Arsenic from Tropical Opencast Coalmine Effluent Through Naturally Occurring Aquatic Macrophytes     

Virendra Kumar Mishra  Alka Rani Upadhyay  Vinita Pathak  B. D. Tripathi 《Water, air, and soil pollution》2008,192(1-4):303-314

Under the present investigation phytoremediation of mercury and arsenic from a tropical open cast coalmine effluent was performed. Three aquatic macrophytes Eichhornia crassipes, Lemna minor and Spirodela polyrrhiza removed appreciable amount of mercury and arsenic during 21 days experiment. Removal capacities of these macrophytes were found in the order of E. crassipes > L. minor > S. polyrrhiza. Translocation factor (shot to root ratio of heavy metals) revealed low transportation of metals from root to leaves leading higher accumulation of metals in root as compared to leaves of the plant. It was evident from plant tissue analysis that mercury and arsenic up take by macrophytes had deteriorated the N, P, K, chlorophyll and protein content in these macrophytes. Correlations between removal of arsenic and mercury from mining effluent and its increase in plant parts were highly significant. Results favoured selected species to use as promising accumulator of metals.  相似文献   

Sustainability of sloping land cultivation systems in the mid‐hills of Nepal     

G. P. Acharya  B. P. Tripathi  R. M. Gardner  K. J. Mawdesley  M. A. Mcdonald 《Land Degradation \u0026amp; Development》2008,19(5):530-541

Maintenance of soil resources is critical in the middle hills of Nepal where the majority of the population are engaged in small‐scale agriculture. However, the causes of declines in soil fertility are complex and related to social change, meaning that farmers are unlikely to adopt soil conservation measures if that is their sole function. In several districts of the middle hills, large, steeply sloping terraces are predominant, so constructed because terrace size is perceived as reflecting wealth and status. They contrast sharply with the flat, narrow terraces characteristic of most middle hill areas. Thus, soil and water management is particularly challenging. This research investigated the efficacy of a combination of legumes, mulch and strip crops in controlling nutrient losses in surface runoff and leachate. Losses of nutrients in leachate exceeded those in runoff by an order of 202 (N), 45 (P) and 27 (K) despite the steep slopes. There was evidence of nutrient scouring associated with soil movement downslope. Income was increased up to threefold as a result of incorporating strips of ginger (Zingiber officinale Rosc.) in the maize‐based systems. The results indicate that low‐input, strip crop technologies are effective in soil and water conservation through the sieve‐barrier effect, while increasing farmers' income and hence have the potential to maintain the overall sustainability of this land‐use system. Copyright © 2008 John Wiley & Sons, Ltd.  相似文献   

Effect of organophosphates in vivo upon acetylcholinesterase isozymes from housefly head and thorax     

R.K. Tripathi  R.D. O&#x;Brien 《Pesticide biochemistry and physiology》1973,2(4):418-424

Four soluble acetylcholinesterase isozymes of head and three from the thorax of housefly (Musca domestica L.) were separated by polyacrylamide gel electrophoresis. Their inhibition in vivo was studied after poisoning with an LD₅₀ of four organophosphates: malaoxon, paraoxon, diazinon and dichlorvos. The isozymes differed greatly in their degree of inhibition. Thoracic isozymes were found to be more sensitive to inhibition than head isozymes. In surviving flies, the recovery rates of head isozymes were much faster than thoracic isozymes. In vitro studies showed that thoracic isozymes differed 4.1- and 2.9-fold in their K_m and V_max.  相似文献   

A note on deoxyribonucleic acid content of bull semen.     

S S Tripathi  V B Saxena 《The Indian journal of animal sciences》1975,45(8):588-589

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Blood of two siluroid fishes during different respiratory conditions     

A K Tripathi  A K Chanchal  B N Pandey  J S Munshi 《Archiv fuer experimentelle veterinaermedizin》1979,33(5):699-702

In the present study an attempt has been made to investigate the changes in different blood parameters in two siluroid fishes, Heteropneustes (H.) fossilis. Bloch and Clarias (C.) batrachus. Linn, both air breathing fishes, during different respiratory conditions, Viz, 1. normal, 2. submerged water condition and 3. exclusive air breathing condition. An increase in different blood parameters [Viz, red blood corpuscles (RBC) counts, haemoglobin (Hb) content and packed cell volume (PCV) etc.] was observed under submerged water and exclusive air breathing conditions. Possible reasons and the mechanism of such changes in different blood parameters have been discussed.  相似文献   

Anti-inflammatory effect of (+)-pinitol   总被引：2，自引：0，他引：2  

Singh RK  Pandey BL  Tripathi M  Pandey VB 《Fitoterapia》2001,72(2):168-170

In the carrageenin-induced paw oedema in rats, (+)-pinitol (2.5-10 mg/kg, i.p.), isolated from Abies pindrow leaves, showed a significant anti-inflammatory effect, the highest dose being comparable to phenylbutazone (100 mg/kg, i.p.).  相似文献