Effect of efficient pectinolytic bacterial isolates on retting and fibre quality of jute     

Biswapriya Das  Kalyan ChakrabartiSagarmoy Ghosh  Bijan MajumdarSudipta Tripathi  Ashis Chakraborty 《Industrial Crops and Products》2012,36(1):415-419

Four bacterial strains with high balanced polygalacturonase (PG) and pectin lyase (PNL), and xylanase with minimal cellulase activity were used in jute retting. The 16S rDNA analysis revealed that the organisms were: Bacillus sp. L6 (GQ891097), Bacillus pumilus strain EK-17 (GQ891098), B. pumilus strain Geo-03-422 (GQ891103) and B. pumilus strain IK-MB12-518F (GQ891105). The microorganisms in different combinations of consortia showed synergistic effect resulting in increased PG (35.52-46.61 IU/g cell wet weight), PNL (39.79-72.12 U/ml), xylanase (0.705-0.840 μmol/ml/min) and little cellulase (0-0.153 μmol/ml/min) activities. The organisms in different combinations of consortia reduced the retting period from 11 to 13 days as compared to 19 days in the control. Microbial inoculation produced remarkable improvement in jute fibre strength (26.62-28.91 g/tex) and fineness (2.76-2.92 tex) over control. The pH of the post-retting water samples became acidic, and the electrical conductivity (Ec), chemical oxygen demand (COD) and hardness increased. The organisms could be adopted in industrial application for extraction of jute fibre.  相似文献   

A rapid technique for screening banana cultivars for resistance to Xanthomonas wilt     

Leena Tripathi  John Odipio  Jaindra Nath Tripathi  Geoffrey Tusiime 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(1):9-19

The banana Xanthomonas wilt disease (BXW) has threatened the livelihood of millions of farmers in East Africa. Use of resistant varieties is the most cost-effective method of managing this bacterial disease. A reliable and rapid screening method is needed to select resistant banana varieties. An in vitro screening method was developed for early evaluation of Xanthomonas wilt resistance using small tissue culture-grown plantlets. Eight cultivars of banana were screened with sixteen isolates of Xanthomonas campestris pv. musacearum using this method. There were significant differences (P < 0.0001) in susceptibility among the various banana cultivars tested, whereas no significant difference (P = 0.92) in pathogenicity was observed between the pathogen isolates. The cv. Pisang Awak (Kayinja) was found to be highly susceptible and Musa balbisiana resistant. Nakitembe was found to be moderately resistant while cvs Mpologoma, Mbwazirume, Sukali Ndiizi, FHIA-17 and FHIA-25 were susceptible. The susceptibility of these cultivars was further tested in vivo by artificial inoculation of potted plants with similar results. This study shows that an in vitro screening test can serve as a convenient, cheap and rapid screening technique to discriminate BXW-resistant from BXW-susceptible banana cultivars.  相似文献   

Acclimation of brackish water pearl spot (Etroplus suratensis) to various salinities: relative changes in abundance of branchial Na+/K+-ATPase and Na+/K+/2Cl− co-transporter in relation to osmoregulatory parameters     

S. Chandrasekar  T. Nich  G. Tripathi  N. P. Sahu  A. K. Pal  S. Dasgupta 《Fish physiology and biochemistry》2014,40(3):983-996

The present study was conducted to elucidate the osmoregulatory ability of the fish pearl spot (Etroplus suratensis) to know the scope of this species for aquaculture under various salinities. Juvenile pearl spot were divided into three groups and acclimated to freshwater (FW), brackish water (BW) or seawater (SW) for 15 days. The fish exhibited effective salinity tolerance under osmotic challenges. Although the plasma osmolality and Na⁺, K⁺ and Cl^? levels increased with the increasing salinities, the parameters remained within the physiological range. The muscle water contents were constant among FW-, BW- and SW-acclimated fish. Two Na+/K+-ATPase α-isoforms (NKA α) were expressed in gills during acclimation in FW, BW and SW. Abundance of one isoform was up-regulated in response to seawater acclimation, suggesting its role in ion secretion similar to NKA α1b, while expression of another isoform was simultaneously up-regulated in response to both FW and SW acclimation, suggesting the presence of isoforms switching phenomenon during acclimation to different salinities. Nevertheless, NKA enzyme activities in the gills of the SW and FW individuals were higher (p < 0.05) than in BW counterparts. Immunohistochemistry revealed that Na⁺/K⁺-ATPase immunoreactive (NKA-IR) cells were mainly distributed in the interlamellar region of the gill filaments in FW groups and in the apical portion of the filaments in BW and SW groups. The number of NKA-IR cells in the gills of the FW-acclimated fish was almost similar to that of SW individuals, which exceeded that of the BW individuals. The NKA-IR cells of BW and SW were bigger in size than their FW counterparts. Besides, the relative abundance of branchial Na⁺/K⁺/2Cl^? co-transporter showed stronger evidence in favor of involvement of this protein in hypo-osmoregulation, requiring ion secretion by the chloride cells. To the best of our knowledge, this is the first study reporting the wide salinity tolerance of E. suratensis involving differential activation of ion transporters and thereby suggesting its potential as candidate for fish farming under different external salinities.  相似文献   

Response of transplanted rice to nitrogen fertilization under different weed management practices     

H. L. Sharma  C. M. Singh  B. Tripathi 《国际虫害防治杂志》2013,59(2):108-110

Abstract

Rice yield increased significantly and consistently up to 120 kg N/ha when weeds were not controlled. But when weeds were effectively controlled by two hand weedings (20 and 40 days after transplanting) or by use of thiobencarb at 1–0 kg a.i./ha rice yield increased significantly up to 80 kg N/ha. The optimum dose of N for transplanted rice under mid‐hill conditions of Himachal Pradesh (Palampur) was 82 kg/ha when two hand weedings were done and 86 kg/ha when weeds were managed using thiobencarb. The yield response per kg of N in the respective treatments was 20 and 24 kg of grain. The weed dry weight was significantly lower in thiobencarb and hand weeded plots compared with unweeded check though the difference in weed dry weight due to the former two treatments was not significant.  相似文献   

Insect pests of stored ergot sclerotia†     

D. Singh  A. K. Tripathi 《国际虫害防治杂志》2013,59(4)

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Allergenicity assessment of the papaya ringspot virus coat protein expressed in transgenic rainbow papaya     

Fermín G  Keith RC  Suzuki JY  Ferreira SA  Gaskill DA  Pitz KY  Manshardt RM  Gonsalves D  Tripathi S 《Journal of agricultural and food chemistry》2011,59(18):10006-10012

The virus-resistant, transgenic commercial papaya Rainbow and SunUp (Carica papaya L.) have been consumed locally in Hawaii and elsewhere in the mainland United States and Canada since their release to planters in Hawaii in 1998. These papaya are derived from transgenic papaya line 55-1 and carry the coat protein (CP) gene of papaya ringspot virus (PRSV). The PRSV CP was evaluated for potential allergenicity, an important component in assessing the safety of food derived from transgenic plants. The transgene PRSV CP sequence of Rainbow papaya did not exhibit greater than 35% amino acid sequence homology to known allergens, nor did it have a stretch of eight amino acids found in known allergens which are known common bioinformatic methods used for assessing similarity to allergen proteins. PRSV CP was also tested for stability in simulated gastric fluid and simulated intestinal fluid and under various heat treatments. The results showed that PRSV CP was degraded under conditions for which allergenic proteins relative to nonallergens are purported to be stable. The potential human intake of transgene-derived PRSV CP was assessed by measuring CP levels in Rainbow and SunUp along with estimating the fruit consumption rates and was compared to potential intake estimates of PRSV CP from naturally infected nontransgenic papaya. Following accepted allergenicity assessment criteria, our results show that the transgene-derived PRSV CP does not pose a risk of food allergy.  相似文献   

Agro-morphological variation in “Jhinuwa” rice landraces (Oryza sativa L.) of Nepal     

Mahendra P. Tripathi  Bhuwon R. Sthapit  Lakshmi P. Subedi  Srawan K. Sah  Sanjaya Gyawali 《Genetic Resources and Crop Evolution》2013,60(8):2261-2271

Jhinuwa is an aromatic rice (Oryza sativa L.) landrace from the Pokhara Valley of Nepal. A total of 210 accessions comprising seven types of Jhinuwa rice landraces were randomly collected from the rice fields to evaluate inter- and intra-population variability based on agro-morphological traits. The experiment was conducted in factorial randomized complete block design with three replicates in 2005. The first six principal components (PCs) accounted for 76.6 % variation for agro-morphological traits. Major traits that accounted for the variation by six PCs includes days to heading, days to maturity, total grain panicle^?1, fertile grain panicle^?1, culm length, panicle length, milling recovery, head rice recovery, aroma, 1,000 grain weight, sterile grain panicle^?1, grain sterility %, and leaf characteristics. Both principal coordinate analysis and cluster analyses revealed four phenotypic groups, two of which represent Bayarni, Jhinuwa, and Biramphul while the other two account for Tunde and Pakho Tunde. Tunde, Pakho Tunde, Kalo Bayarni, and Seto Bayarni showed higher intra- as well as inter-population variation compared to other populations. The phenotypic and genotypic coefficients of variation, broad sense heritability (h ²B) and genetic advance (GA) as a percent of the mean assessed for 210 accessions revealed high h ²B and GA estimates for leaf width, leaf length breadth ratio, ligule length, sterile grain panicle^?1, grain sterility % and 1,000 grain weight. The current study demonstrates that improvement in Jhinuwa rice landrace is possible by selecting superior accessions from existing natural populations while selection should be focussed to market traits with higher h ²B and GA estimates.  相似文献   

一些竹种在不同季节不定根诱导的形式     

Singh S. Nain N.P.S. Meena S.L. Tripathi S. E 《世界竹藤通讯》2008,(3):48-48

考察了季节和植物激素、非植物激素生长调节剂对不定根形成的影响,以进行黄竹、孝顺竹、马甲竹和龙竹无性繁殖。在冬天,夏天和雨季的3个生长季节,将成熟秆截取成单节扦插方式,并用IAA,IBA、NAA、硼酸和水进行24h处理。之后,将扦插秆水平放置,盖上沙子,在喷雾箱中放2个月。  相似文献   

Nutrient losses from rain‐fed bench terraced cultivation systems in high rainfall areas of the mid‐hills of Nepal     

G. P. Acharya  M. A. McDonald  B. P. Tripathi  R. M. Gardner  K. J. Mawdesley 《Land Degradation \u0026amp; Development》2007,18(5):486-499

Between the elevations of 1000 and 2000 m in the mid‐hills of Nepal, over 12 million people subsist on land‐holdings of less than 0·5 ha. These farmers have limited access to commercial inputs such as fertilisers and are reliant on organic manures for soil fertility maintenance. Participatory research was conducted with farmers on bari land (upper slope rain‐fed crop terraces) in the hill community of Landruk (bench terraces 0–5° slope, 3000–3500 mm annual rainfall, which aimed to develop soil and water management interventions that controlled erosion without resulting in high leaching, and so were effective in minimising total nutrient losses. Interventions tested were the control of water movement through diversion of run‐on and planting fodder grasses on terrace risers on bench terraces. The interventions were effective in reducing soil loss from the bari land in comparison with existing farmer practices, but no effect was observed on nutrient losses in solution form through runoff and leaching. Losses of NO₃‐N in leachate ranged from 17·3 to 99·7 kg ha⁻¹ yr⁻¹, but only 0·7 to 5·6 kg ha⁻¹ yr⁻¹ in runoff. The overall nutrient balance suggests that the system is not sustainable. Fertility is heavily dependent on livestock inputs and if the current trends of declining livestock numbers due to labour constraints continue, further losses in productivity can be expected. However, farmers are interested in interventions that tie ecosystem services with productivity enhancement and farmers' priorities should be used as entry points for promoting interventions that are system compatible and harness niche opportunities. Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

Clinicopathological findings of an episode of mycotoxicosis in horses     

R. K. Dedar  N. Virmani  P. A. Bala  J. Singh  R. K. Vaid  R. A. Legha  B. N. Tripathi 《Equine Veterinary Education》2019,31(5):236-241

This report of mycotoxicosis in horses describes the clinical signs, post-mortem findings, histopathological findings and prognosis following the accidental feeding of mycotoxin contaminated fodder to the horses at a farm over a 3-year period. Variable clinical signs viz. weight loss, height retardation in foals, episodes of sudden weakness and staggering gait, ulceration in the oral cavity and at mucocutaneous junctions, yawning, decreased appetite to complete anorexia, rectal prolapse, and failure of blood clotting were observed in many horses at an organised equine farm over a period of 2–3 years. Post-mortem examination of these cases revealed liver cirrhosis and pathological changes in other organs too. On examination of the fodder, it was observed that pearl millet fodder fed to the animals had ergot infestation. On laboratory investigation of the fodder, aflatoxins levels ranged from 24 ppb to 70 ppb in pearl millet fodder. The analysis eventually led to discontinuation of the pearl millet fodder. On follow-up for 2 years, no new cases of hepatic cirrhosis were seen. Most of the horses at the farm including those that had shown high levels of serum gamma glutamyl transferase (GGT) and weight loss, recovered completely. The present episode demonstrates that there is a need for regular monitoring of cereal fodders of horses in subtropical and tropical areas to avoid mycotoxicosis. In the present episode, monitoring serum/plasma GGT levels was found to be a sensitive biochemical indicator to identify liver damage caused by mycotoxins.  相似文献