Effect of Alternate Wetting and Soil Drying Irrigation during Grain Filling on the Physiological Traits and Yield of Transgenic Rice with Different Protein Content in Grains     

LIU Li jun  WANG Kang jun  BIAN Jin long  XIONG Yi wei  WANG Zhi qin  YANG Jian chang 《中国水稻科学》2014,28(4):384

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Overexpression of Dehydroascorbate Reductase Confers Enhanced Tolerance to Salt Stress in Rice Plants (Oryza sativa L. japonica)     

Y. S. Kim  I. S. Kim  S. Y. Shin  T. H. Park  H. M. Park  Y. H. Kim  G. S. Lee  H. G. Kang  S. H. Lee  H. S. Yoon 《Journal of Agronomy and Crop Science》2014,200(6):444-456

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CAD／CAM集成数控自动编程系统的研制     

刘成良  陈琦 《山东农业大学学报(自然科学版)》1993,24(1):79-87

SAAPT-2(1/2)D NC自动编程系统能高效、准确地实现零件源程序到NC程序、NC穿空纸带的转换。该系统由几何处理模块、工艺处理模块、后置处理模块和子程序库组成。几何处理模块对零件源程序几何定义语句进行词法、语法检查和参数标准化计算;工艺处理模块根据标准化参数对运动语句及其它非几何定义语句进行处理,生成中间代码文件;后置处理模块将中间代码文件变成NC加工程序。  相似文献   

青枯菌无毒自发突变株接种花生引起的生化变化     

康耀卫  何礼远 《中国油料作物学报》1994,(1)

花生经青枯菌无毒自发突变株ＡＰ７接种后，体内过氧化物酶的活性会增高。经无毒自发突变菌株ＡＰ７接种后的花生叶片组织粗提液对青枯菌的致病菌株有强烈的体外抑菌作用。将这种粗提液与致病菌株同时注入花生叶片内，亦表现出抑制发病作用，而且这种抑病作用随着粗提液浓度下降而减弱．  相似文献   

维生素 C 保护稻叶细胞膜机理的研究   总被引：1，自引：0，他引：1  

冯有胜  王康 《西南大学学报(自然科学版)》1988,(1)

本文研究了维生素 C 清除超氧自由基(0),保护细胞膜的机理。结果表明;V—C 有清除 O的效应。在清除 O的过程中,有过氧化氢的生成,并且可以经过清除 O而对脂肪酸起到一定的保护作用。同时,V—C 能促进芬顿(Fenton)反应而加快脂肪酸的过氧化破坏作用。用9ppm 的 V—C 喷洒处于40℃和40000lx 条件下的稻叶,能显著降低膜脂过氧化作用和减轻细胞膜的损伤程度。但当喷洒浓度超过9ppm 后,其保护效果减弱。此外,当细胞内 V—C 浓度提高后,过氧化氢酶活性随之增强。  相似文献   

亚硒酸钠一次内服在犊牛体内药动学研究   总被引：2，自引：0，他引：2  

康世良  王振隆 《中国兽医学报》1995,15(2):121-124

给犊牛一次内服（０．６ｍｇ／ｋｇ体重）亚硒酸钠溶液后不同时时间采集血样，用荧光分光光度计测定血硒浓度，在ＩＢＭ微机上用ＭＣＰＫＰ－微机用药动学程序软件处理血硒浓度实测值，微机输出血硒浓度理论值及药动学参数。血硒浓度－时间曲线符合二室开放模型，最佳药时方程为：Ｃｉ＝０．３６０２ｅ＾－４．６７５３ｔ＋０．４６２２ｅ＾－０．０００７ｔ－（０．３６０２＋０．４６２２）ｅ＾－０．１１７２ｔ．主要动力学参数：  相似文献   

Rapid coagglutination test for detection of rotaviruses in turkeys     

S Y Kang  K V Nagaraja  J A Newman 《Avian diseases》1985,29(3):640-648

Avian rotaviruses present in fecal samples were readily detected using a staphylococcal protein-A coagglutination test on a white porcelain plate. Staphylococci, which produced large amounts of protein-A, were coated with rabbit anti-avian rotavirus serum. The antibody-coated staphylococci were agglutinated specifically by rotavirus present in the fecal sample. The macroscopic agglutination reaction occurred within a few minutes. A total of 40 fecal samples were tested by the coagglutination test. The sensitivity and specificity of the coagglutination test were compared with those of electron microscopy, enzyme-linked immunosorbent assay, and tissue-culture virus-isolation methods. Of the 31 fecal samples positive for rotavirus on electron microscopy, 27 (87%) were positive on coagglutination test. Of the nine electron-microscopy-negative samples, seven (78%) were also negative on coagglutination test. It was concluded that the staphylococcal protein-A coagglutination test can be used as a simple, rapid screening test for avian rotavirus.  相似文献   

低温与稻苗超氧物歧化酶活性变化及细胞膜损伤的关系     

谭德勇  王康 《西南大学学报(自然科学版)》1988,(1)

用不同的低温(2℃、6℃、10℃)处理水稻幼苗后,又于25℃,4000lx 光照下培养3天,分别研究低温期间的“冷效应”和低温后常温期间的“冷后效应”中,起氧物歧化酶(SOD)的活性变化及其与细胞膜损伤的关系,结果表明:低温期间 SOD 活性没有明显变化,脂质过氧化作用不是细胞膜损伤的主要原因。但在冷后效应中,膜脂质过氧化作用增强和 SOD 活性下降与细胞膜损伤有密切关系。同位素示踪表明,SOD 活性下降的主要原因是它的生物合成受阻。  相似文献   

Effects of PTEN on apoptosis,oxidative damage and immune inflammatory factors in myocardial H9c2 cells with anoxia/reoxygenation     

YAN Lin-zhen  YAN Qin-dong  ZHANG Shun-xiang  DING Xiao-qing  LI Kang 《园艺学报》2018,34(11):1940-1946

AIM: To investigate the effects of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) on the apoptosis, oxidative damage and immune inflammatory factors in myocardial H9c2 cells with anoxia/reoxygenation (A/R). METHODS: The H9c2 cells were used to establish a model of A/R. The H9c2 cells were transfected with PTEN small interfering RNA (siRNA) and negative control. After A/R, the expression of PTEN at mRNA and protein levels was determined by RT-PCR and Western blot, respectively. The cell viability was measured by MTT assay. The apoptosis was analyzed by flow cytometry. Xanthine oxidase method was used to determine superoxide dismutase (SOD) activity. The content of malondialdehyde (MDA) was detected by thiobarbituric acid method. The lactate dehydrogenase (LDH) activity in the supernatant was evaluated by 4-dinitrophenylhydrazine method. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 in culture supernatant were examined by ELISA. The protein levels of cleaved caspase-3, Bax and FasL in the cells were determined by Western blot. RESULTS: After A/R, the expression of PTEN at mRNA and protein levels was significantly increased in the H9c2 cells (P<0.05). The mRNA and protein levels of PTEN were decreased significantly after transfection with PTEN siRNA (P<0.05). The viability of H9c2 cells was decreased after A/R, while the apoptotic rate was increased. The protein levels of cleaved caspase-3, Bax and FasL were increased in the cells. The MDA level was elevated, the activity of SOD was decreased, and the levels of LDH, TNF-α, IL-1β and IL-6 in the culture supernatant were increased (P<0.05). Down-regulation of PTEN partly antagonized the effects of A/R on the viability, apoptotic rate, MDA content, SOD activity, and the levels of LDH, TNF-α, IL-1β and IL-6 in culture supernatant. CONCLUSION: Down-regulation of PTEN attenuates oxidative damage induced by A/R, reduces apoptosis and secretion levels of TNF-α, IL-1β and IL-6 in the H9c2 cells.  相似文献   

影响野生杏种子萌发的相关因素研究初报   总被引：2，自引：0，他引：2  

刁永强  廖康  许正  王庆  杨磊  耿文娟  陈云华 《北方园艺》2008,(4):27-30

试验以新疆野生杏的单株种子为材料,通过低温层积、机械去壳、去种皮及赤霉素处理等方法,研究不同处理对野生杏种子休眠及萌发的影响.结果表明:野生杏种子经过低温层积处理后,在一定时间内,随着层积时间的延长,发芽率也随之提高;低温层积处理40 d后野生杏种子开始萌发,100 d后发芽率可达90%;野生杏种子的种壳和种皮不同程度的抑制了种子萌发,去除种壳可使种子萌发提前,同时提高其发芽率;未层积的种子在去除种皮后用清水处理24 h,在25℃条件下,种子的萌发率达到80%以上,说明去除种皮能基本解除种子的休眠;一定浓度的GA3对野生杏种子的萌发具有促进作用.6号带壳种子与去壳种子的最适GA3浓度均为100mg/L,发芽率分别为86.7%、100%.而7号带壳种子的最适GA3浓度为300 mg/L,发芽率为70%.  相似文献