Assessment of “discreet” vertebral abnormalities, bone mineralization and bone compactness in farmed rainbow trout   总被引：1，自引：1，他引：0  

M.-H. Deschamps  A. Kacem  R. Ventura  G. Courty  P. Haffray  F.J. Meunier  J.-Y. Sire   《Aquaculture (Amsterdam, Netherlands)》2008,279(1-4):11-17

The present study aimed to evaluate the importance of “discreet” vertebral abnormalities in normally-shaped rainbow trout in relation to vertebral bone condition in French fish farms. A total of 373 trout (262 ± 2 mm in total length) from 20 fish farms sampled were studied. The fish were radiographed and the axial skeleton examined for vertebral abnormalities. Vertebrae from the middle axial region (V32-38) were selected to evaluate vertebral bone condition. Bone mineralization (BM, %) was estimated by the ratio of ash and dry weight. Bone compactness (BC,%) was measured using Bone Profiler 3.23 images software on digitized radiographs of transverse sections (125 ± 10 μm). Statistical analyses were performed to test the relationships between the occurrence of vertebral abnormalities, and BM and BC. The occurrence of affected trout ranged from 0 to 55% depending on the farm. Trout displayed vertebrae with low BM (< 54.6%) and low BC (< 28.1%) in 40% and 55% of the farm, respectively. No relationships were observed between bone condition parameters (BM, BC) and the occurrence of vertebral abnormalities. These results could be explained by a wide and variable plastic response of bone characters (i.e., vertebral abnormalities, BM and BC) to the various rearing conditions in the fish farms sampled.  相似文献   

The Os Coxae of a Digging Form of the Northern Water Vole, Arvicola terrestris (Rodentia, Arvicolidae)     

J. Ventura    J. Gosálbez  V. J. Götzens 《Anatomia, histologia, embryologia》1991,20(3):225-236

Data on the morphology, relative growth and sexual dimorphism of the os coxae of a digging form of the northern water vole Arvicola terrestris (A.t. monticola) are given. Two hundred ninety-nine specimens (146 males and 153 females) caught in the Aran Valley (Spanish Pyrenees) were analyzed. The specimens were distributed into six classes of relative age, according to the skull morphology, kind of coat, moulting state, weight and sexual state. The morphological features of the os coxae in these specimens are similar to those described in other species of Arvicolidae. The hormonal effect upon the pelvis in adult females during pregnancy and parturition leads to the appearance of marked intersexual differences in the obturator foramen length, the pubis length, the pelvis length and, specially, in the pelvis height and the pubis width. By means of Becker's and Dunmire's procedures both sexes can be correctly discriminated in a 78.87 and a 76.4% respectively. Conjugating the variation patterns obtained with Becker's index and the pelvis height, we have obtained a more effective method of sexual differentiation (an 85.51% of correct discrimination).  相似文献   

Indirect ELISA for the diagnosis of brucellosis in water buffaloes (Bubalus bubalis) in Italy     

Guarino A  Fusco G  Di Matteo A  Urbani G  Condoleo R  Serpe L  Tittarelli M  Di Ventura M  Gallo P 《The Veterinary record》2001,149(3):88-90

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Selenium Levels in Mainland Portugal     

Márcia G. Ventura  Maria Do Carmo Freitas  Adriano M. G. Pacheco 《Water, air, and soil pollution》2005,166(1-4):167-179

Selenium contents in the Portuguese (mainland) territory, resulting from a few environmental studies held since 1990 using lichens, vascular plants, soils and air particulate matter (APM), are presented and discussed herein. Values for Se in soils, epiphytic lichens and higher plants are within the typical ranges found in the literature. Compared to vascular plants, lichens display higher contents at the same locations. In what concerns Se in airborne particulates, all surveyed sites show values that are typical of urban areas. In two of such sites – Monchique and Carregado – Se concentrations in transplanted lichen thalli of the Parmelia genus (mostly, Parmelia sulcata Taylor) were compared to the results obtained from airborne matter, and a good correlation was found for the corresponding data-sets at either location. Other than the degree of association, it is especially noteworthy that the lichen transplants appear to accumulate much more Se at Monchique (a remote, rather clean site) than at Carregado (in the immediate vicinity of an oil-fired, power plant), even if its amount in the atmosphere is lower at the former location.  相似文献   

Analysis of ochratoxin a in coffee by solid-phase cleanup and narrow-bore liquid chromatography-fluorescence detector-mass spectrometry     

Ventura M  Vallejos C  Anaya IA  Broto-Puig F  Agut M  Comellas L 《Journal of agricultural and food chemistry》2003,51(26):7564-7567

A method for the analysis of ochratoxin A (OTA) in green and roasted coffee has been developed. OTA was extracted from coffee with 1% NaHCO(3), and the extract was filtered and purified by solid-phase cleanup using a polymeric column that exhibits reversed-phase and anion-exchange functionalities. OTA was analyzed on a narrow-bore reversed-phase C(18) HPLC column with acetonitrile/water (0.1% formic acid) (40:60) as mobile phase and quantified with a fluorescence detector. The presence of OTA in coffee was confirmed by single-quadruple mass spectrometry using an electrospray ionization source. The method has been validated, obtaining a recovery of 82.5% and a detection limit of 0.1 ng/g. It has been applied to 20 coffee samples from various countries and different manufacturers with no detection of OTA.  相似文献   

Apa antigen of Mycobacterium avium subsp. paratuberculosis as a target for species-specific immunodetection of the bacteria in infected tissues of cattle with paratuberculosis     

Souza GS  Rodrigues AB  Gioffré A  Romano MI  Carvalho EC  Ventura TL  Lasunskaia EB 《Veterinary immunology and immunopathology》2011,143(1-2):75-82

Comparative genomics of Mycobacterium spp. have revealed conservative genes and respective proteins differently expressed in mycobacteria that could be used as targets for the species-specific immunodiagnostics. The alanine and proline-rich antigen Apa is a mycobacterial protein that present significant variability in primary sequence length and composition between members of M. avium and M. tuberculosis complexes. In this study, the recombinant Apa protein encoded by the MAP1569/ModD gene of M. avium subsp. paratuberculosis (Map) was used to generate a panel of monoclonal antibodies which were shown to recognize the most important veterinary pathogens of the M. avium complex, specifically Map and M. avium subsp. hominissuis, and which did not cross-react with M. bovis or M. tuberculosis. The produced antibodies were demonstrated to be a useful tool for the species-specific immunofluorescence or immunohistochemical detection of Map in experimentally infected cell cultures or intestinal tissues from cattle with bovine paratuberculosis and, additionally, they may be employed for the discrimination of pathogenic M. avium subspecies via Western blotting.  相似文献   

A genomewide association mapping study using ultrasound‐scanned information identifies potential genomic regions and candidate genes affecting carcass traits in Nellore cattle          下载免费PDF全文

M.H.A. Santana  R.V. Ventura  Y.T. Utsunomiya  H.H.R. Neves  P.A. Alexandre  G.A. Oliveira Junior  R.C. Gomes  M.N. Bonin  L.L. Coutinho  J.F. Garcia  S.L. Silva  H. Fukumasu  P.R. Leme  J.B.S. Ferraz 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2015,132(6):420-427

The aim of this study was to identify candidate genes and genomic regions associated with ultrasound‐derived measurements of the rib‐eye area (REA), backfat thickness (BFT) and rumpfat thickness (RFT) in Nellore cattle. Data from 640 Nellore steers and young bulls with genotypes for 290 863 single nucleotide polymorphisms (SNPs) were used for genomewide association mapping. Significant SNP associations were explored to find possible candidate genes related to physiological processes. Several of the significant markers detected were mapped onto functional candidate genes including ARFGAP3, CLSTN2 and DPYD for REA; OSBPL3 and SUDS3 for BFT; and RARRES1 and VEPH1 for RFT. The physiological pathway related to lipid metabolism (CLSTN2, OSBPL3, RARRES1 and VEPH1) was identified. The significant markers within previously reported QTLs reinforce the importance of the genomic regions, and the other loci offer candidate genes that have not been related to carcass traits in previous investigations.  相似文献   

Effect of phosphorus concentration in irrigation water on the development of jojoba cuttings     

Aliza Benzioni  Moshe Ventura 《Journal of plant nutrition》2013,36(12):2697-2706

The effect of different phosphorus (P) levels in irrigation water on root and shoot development and elemental composition was studied in plantlets of two clones of jojoba, 64 and 879–154. High P in the irrigation water inhibited root development in both clones but to a greater degree for clone 879–154. Low P resulted in a decrease in magnesium (Mg) and calcium (Ca) content in the leaves, but had no effect on shoot growth or chlorophyll concentration.  相似文献   

The invasive mealybug Maconellicoccus hirsutus: lessons for its current range expansion in South America and invasive pest management in general     

Mark P. Culik  Maurício José Fornazier  David dos Santos Martins  José Salazar Zanuncio Jr.  José Aires Ventura  Ana Lúcia B. G. Peronti  José Cola Zanuncio 《Journal of pest science》2013,86(3):387-398

The invasive mealybug Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) is a plant feeding insect believed to be native to Southern Asia or Australia. This mealybug has become established in many regions throughout the world (including the Caribbean and North America) in the past 100 years and is currently expanding its range in South America. Because this insect is of concern as a potential pest of many plant species, this review is provided to summarize knowledge of M. hirsutus based on past research that may be most useful for addressing the current invasion of South America by the species, and to identify gaps in information that may need to be addressed to inhibit the spread of the insect and improve management methods for this and similar organisms. In most areas into which M. hirsutus has expanded its range it is commonly suppressed by native or introduced natural enemies. Therefore, besides preventing introduction, establishment and spread of this potential pest, efforts should be made to determine if natural enemies of M. hirsutus are present in areas of concern (i.e., where the mealybug may be introduced and become established). Such information will enable determination of the need for introduction or augmentation of biological control agents in response to possible entry and establishment of the mealybug in new areas. Methods developed in response to the recent invasion of the Caribbean and North America by M. hirsutus may serve as models for addressing the threat of this and similar invasive pests in South America and elsewhere.  相似文献   

Long-term effect of irrigation with saline water on the development and productivity of jojoba clones     

Aliza Benzioni  M. Ventura  Y. De Malach 《The Journal of Horticultural Science and Biotechnology》2013,88(6):835-846

Summary

Three commercial clones of jojoba (Simmondsia chinensis) (64, Q-106 and 879–154) were planted in June 1991 at the Ramat Negev Experimental Station (Israel) and fertigated with water at three salinity levels, 1.2, 3.4 or 6.2 dS m^?1. The effects of salinity over three and a half years on key metabolic activities, on plant growth and development, on flowering characteristics, and on yield were studied. Salinity barely inhibited the rate of net photosynthesis, although it did reduce stomatal conductivity by about 50%. Salinity also affected the water status of the plants by reducing water potential. Growth of the plants was not greatly affected by salinity. It was found that jojoba plants accumulate sodium and chloride ions in their leaves and use a strategy typical of many halophytes to cope with the salinity. The effect of salinity on flower development was also studied, since the vitality of flowers is a key factor in obtaining seeds. Salinity did not affect the timing of the breaking of flower bud dormancy in the 1992-93 season, although it did delay flower bud growth in clones Q-106 and 64. Salinity brought forward the flowering of clone 879–154 by about a fortnight in the 1993-94 season, but did not affect the flowering pattern of the other clones. Fruit set was not inhibited by salinity in the 1994 or 1995 season, and no significant effects were found on yield and wax content in 1994 season, but yield was reduced by salinity in the 1995 season.  相似文献