Effect of dexamethasone on the expression of aquaporin-1 in cultured rat pleural mesothelial cells     

ZHANG Wei  XIE Can-mao  HE Qiao  DENG Xiao-hua  KE Cai-xia 《园艺学报》2007,23(1):22-26

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GM-CSF induces human vascular endothelial cells to form vessel-like structure and the role of VEGF     

ZHAO Wei  YANG Xiang-hong  WANG Yan-lin  WANG Yue-zhong  ZHANG Ya-jia 《园艺学报》2007,23(4):682-685

AIM: To investigate the influence of GM-CSF on human vascular endothelial cells induced to form new blood vessels and the role of VEGF. METHODS: HUVECs were cultured by Matrigel to set up a stable angiogenesis system with the stimulating factors. The rhGM-CSF concentration-dependent and time-dependent effects and the role of VEGF165 were detected. CD34 was measured by immunochemical staining and numbers of vessel formation was calculated under microscopic observation. RESULTS: After treatment with rhGM-CSF at various concentrations and at different time points, the numbers of vessel formation increased in a dose-dependent and time-dependent manner. In the presence of VEGF165, the numbers of vessel formation increased evidently. CONCLUSION: HUVECs were induced to develop tubular structure in vitro cultured with Matrigel. GM-CSF promotes human vascular endothelial cells to form vessel-like structure in vitro in a dose-dependent and time-dependent manner. VEGF also in vitro promotes human vascular endothelial cells to form new vessel-like structure.  相似文献   

Effect of Ginkgo biloba extract on the expression of connective tissue growth factor in the initiating stage of fibrosis in lungs of rats     

HUANG Yan-min  CHEN Xiao-ling  XU Ning  LIU Wei  QI Jie  LIU Jian-hui  AI Jie 《园艺学报》2007,23(6):1130-1132

AIM: To study the effect of Ginkgo biloba extract (GbE) on the expression of connective tissue growth factor (CTGF) in the initiating stage of pulmonary fibrosis of rats after administration of bleomycin (BLM).METHODS: The expression of CTGF in lungs was detected by Western blotting. The content of hydroxyproline was assayed by the method of chloramines T. The content of malondialdehyde (MDA) in plasma was investigated by colorimetry.RESULTS: On day 14 after administration of BLM, the contents of CTGF in lungs and MDA in plasma in BLM+NS group were higher than those in NS group, respectively (P<0.05; P<0.01). On day 30 after BLM, the contents of hydroxyproline in lungs and MDA in plasma in BLM+NS group were higher than those in NS group, respectively (both P<0.01). Treatment with GbE ameliorated the above changes induced by BLM. CONCLUSION: GbE ameliorates the up-regulation of CTGF in the initial stage of fibrosis in lungs of rats after administration of BLM. GbE prevents the hyperoxidative injury in lungs of rats after BLM, which might be one of mechanisms underling the effect of GbE on CTGF.  相似文献   

Effects of FKBP12.6 overexpression on the performance of sarcoplasmic reticulum in ventricular myocytes of rats with heart failure     

LI De  WU Wei  LUO Ning  ZHOU Shu-xian  FANG Chang 《园艺学报》2007,23(7):1263-1266

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Potassium channel gene expression in myocardial sleeves of pulmonary vein and effects of amiodarone on rabbits with rapid atrial pacing     

WANG Jiang-rong  HOU Ying-long  ZHOU Liao-sheng  GAO Mei  SHI Chang-wen  WANG Qing  SUN Ying-ying  LIU Wei 《园艺学报》2007,23(8):1476-1479

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Characteristics of ventricular electrophysiology in a right ventricular rapid pacing-induced canine heart failure model     

ZHOU Shu-xian  WU Wei  ZHANG Yu-ling  LEI Juan  ZHANG Xu-ming 《园艺学报》2007,23(11):2116-2121

AIM: To research the characteristics of ventricular electrophysiology in right ventricular rapid pacing-induced congestive heart failure (CHF) dogs.METHODS: Dogs (n=16) were randomly divided into 2 groups: the control (n=7) and the CHF group (n=9) induced by rapid right ventricular pacing at 240 pulse·min-1 for 4 to 5 weeks.The electrophysiologic parameters were evaluated by the technique of standard electric stimulation and monophasic action potential (MAP) recording.RESULTS: (1) Ventricular effective refractory period (VERP),ventricular MAP duration (MAPD90),ventricular late repolarization duration (VLRD) and intra-ventricular conduction time (IVCT) were prolonged by 26% (P<0.01),43% (P<0.01),318% (P<0.05),and 19% (P<0.01),respectively in CHF group.(2)The ratio of VERP to MAPD₉₀ (VERP/MAPD₉₀) was decreased by 13% (P<0.05) in CHF group.(3) The dispersion of ventricular recovery time (VRT-D) was increased by 185% (P<0.01) in CHF group.(4) The ventricular fibrillation threshold (VFT) was decreased by 48% (P<0.01) in CHF group.CONCLUSION: The abnormal electrophysiological changes in the CHF condition may be contributing factors of lethal ventricular arrhythmias and sudden cardiac deaths in CHF.  相似文献   

Screening differentially expressed genes involved in apoptosis of primary cultured rat cerebellar granule neurons by mRNA differential display RT-PCR     

YIN Wei  HUANG Yi-jun  PI Rong-biao  SU Xing-wen  ZHAO Ling-zhi  QIU Peng-xin  YAN Guang-mei 《园艺学报》2007,23(11):2141-2146

AIM: To Screen and identify differentially expressed genes that involved in apoptosis model in rat cerebellar granule neurons (CGNs).METHODS: The rat cerebellar granule neurons were isolated and primarily cultured.Fluorescent differential display RT-PCR (FDD RT-PCR) was performed to screen differentially expressed ESTs in the apoptosis model of primarily cultured rat CGNs.ESTs were subcloned into pGEM-T EasyTM vector and then sequenced.Alignment assay in non-redunant database was applied for encoding information.Reverse Northern blotting was used to appraise the results from DDRT-PCR.RESULTS: 164 pieces of differentially expressed ESTs were obtained by FDDRT-PCR.17 of them were subcloned and sequenced.5 ESTs of 17 were confirmed to be positive results by reverse Northern blotting.CONCLUSION: DD-PCR is a rapid,simple-operation and sensitive method for screening differentially expressed genes,which would contribute to the molecular mechanisms of apoptosis/survive of CGNs.  相似文献   

Effect of preoperative transarterial chemoembolization on nephroblastoma     

LI Jia-ping  ZHOU Qi  LI Zhi  CHEN Wei  YANG Jian-yong 《园艺学报》2007,23(11):2195-2199

AIM: To explore the effect and mechanism of preoperative transarterial chemoembolization on nephroblastoma.METHODS: Comparative analysis of clinical and pathological features in 39 children with Wilms’ tumor was conducted.TUNEL assay was used to detect the apoptosis of tumor in two groups with or without preoperative interventional treatment.The expressions of P53,Bcl-2 and Bax proteins were detected by immunochemical methods.The patients were followed-up for more than 2 years.RESULTS: The extent of neoplastic cell necrosis and degeneration,interstital fiber tissue hyperplasia of tumor and the number of infiltrating lymphocytes were observed,which were higher in interventional group than those in simple excision group (P<0.01).The median of mitotic index in tumor cells was 0.2 in interventional group,and 1.4 in simple excision group.The median of apoptotic index (AI) of the tumor cells was 25.9 in study group and 12.8 in control group (Uc=54.50,P<0.01),(Uc=117.00,P<0.05).The comparison between the means obtained in two groups revealed statistical difference (P<0.05).The expressions of P53 and Bcl-2 proteins were not correlated with apoptotic index,but the rate of expression of Bax protein (80.0%) in interventional group was higher than that (40.0%) in simple excision group.The two year event-free rate was 73.3％ (11/15) in study group and 42.9％ (6/14) in control group.CONCLUSION: Transarterial chemoembolization on nephroblastoma is effectively used to kill and inhibit tumor cell growth,as well as induce tumor cell apoptosis by Bax protein.  相似文献   

低温胁迫对东方山羊豆幼苗生理特性的影响     

沙伟  师帅  罗新义  荣姝 《北方园艺》2007,(11):59-60

以东方山羊豆及敖汉苜蓿为材料,研究低温胁迫对东方山羊豆和敖汉苜蓿可溶性蛋白含量、保护酶活性、渗透调节物质的影响.结果表明:在低温胁迫下,东方山羊豆及敖汉苜蓿脯氨酸含量、SOD、POD活性、可溶性蛋白含量先上升后下降.经对几项生理指标的综合分析,认为东方山羊豆比敖汉苜蓿具较强的抗低温能力.  相似文献   

阿魏侧耳多糖研究进展   总被引：2，自引：1，他引：2  

闫训友  王玮  赵英 《北方园艺》2007,(12):243-245

阿魏侧耳是一种具有药用价值的名贵珍稀食用菌,具有"天然、营养、保健"于一体功能.现对阿魏侧耳的研究历史及阿魏侧耳多糖的药用价值进行综述.  相似文献