Characterization of the bovine immunoglobulin lambda light chain constant IGLC genes     

Chen L  Li M  Li Q  Yang X  An X  Chen Y 《Veterinary immunology and immunopathology》2008,124(3-4):284-294

To characterize the bovine immunoglobulin lambda light chain constant region (IGLC) genes, we have isolated a bacterial artificial chromosome (BAC) clone by a PCR based approach from a bovine genomic DNA library, constructed using a genital ridge cell line derived from a male Holstein fetus. The positive BAC clone, containing the bovine IGLC genes, was fully sequenced and had a 138 kb insert. Sequence analysis revealed that the bovine immunoglobulin lambda light chain locus consisted of four joining-constant gene recombination units spanning approximately 20 kb DNA in length. A detailed examination of the recombination signal sequences, RNA splicing sites and coding sequences of the four joining-constant gene recombination units suggested that only two IGLC genes (IGLC2 and IGLC3) were functional while the IGLC1 and IGLC4 appeared to be pseudogenes. This conclusion was further confirmed by a series of RT-PCR amplifications, which also showed that among these four genes the IGLC3 was preferentially expressed in cattle. Phylogenetic analysis indicated that the bovine IGLC genes were more closely related to their equivalents in sheep and goats than that to other mammals.  相似文献   

Multi-trait and random regression approaches for addressing the wide range of weaning ages in Asturiana de los Valles beef cattle for genetic parameter estimation     

Menéndez-Buxadera A  Carleos C  Baro JA  Villa A  Cañón J 《Journal of animal science》2008,86(2):278-286

Weaning weight (WW) records of 24,066 Asturiana de los Valles beef cattle, including the progeny of 557 sires and 10,653 dams, were analyzed using a multitrait animal model (MAM) and a random regression model (RRM) in order to estimate the variance components and the breeding value of the animals. Three definitions of WW were used: early weaning (EW) for animals weaned before the age of 180 d; standard weaning (SW) for animals weaned between 180 and 240 d old; and late weaning (LW) for animals weaned between the ages of 240 and 365 d. The heritabilities (h(2)) were high (from 0.49 to 0.63), which fully agrees with previous estimates for this breed. The genetic correlations between EW and SW were 0.86, and lower between EW and LW at 0.543 to 0.622, using MAM and RRM models, respectively. Ranking of sires displayed changes depending on age at weaning of their offspring, which could explain the modest genetic progress reached using MAM evaluation. The first 2 eigenvalues of the random regression coefficient matrix explained 66 and 30% of the genetic variance, which implies important genetic variation underlying the form of the growth curve of the animals during the weaning period. The evaluation of sires according to the official method, as currently carried out by the breeders' association (WW adjusted previously to the age of 180 d), does not exploit the genetic differences in response to their production system where the calf is weaned at variable ages.  相似文献   

Pharmacokinetics of altrenogest in gilts     

Hongzhi Xiao  Pan Sun  Feifei Sun  Jicheng Qiu  Jie Wang  Jianzhong Wang  Yalong Lin  Xiaohui Gong  Lu Zhang  Suxia Zhang  Xingyuan Cao 《Journal of veterinary pharmacology and therapeutics》2019,42(6):660-664

Altrenogest, a synthetic progestogen, is characterized by its estrus synchronization in mares, ewes, sows, and gilts. To investigate the pharmacokinetic profile and evaluate its accumulation in gilts, 18 oral doses of 20 mg altrenogest/gilt/day were given to eight healthy gilts at an interval of 24 hr. Plasma samples were collected, and altrenogest was determined by ultra‐high‐performance liquid chromatography with mass spectrometry. WinNonlin 6.4 software was used to calculate the pharmacokinetic parameters through noncompartmental model analysis. After the first administration (D 1), the pharmacokinetic parameters, including T_max, C_max, and the elimination half‐life (T_1/2λz), were similar to those observed after the final administration (D 18). However, the mean residence time at D 1 was significantly lower than D 18. As a whole, the mean steady‐state plasma concentration (C_ss), degree fluctuation (DF), accumulation factor (R_ac), and area under the plasma concentration–time curve in steady state (AUC_ss) were 22.69 ± 6.15 ng/ml, 270.64 ± 42.51%, 1.53 ± 0.23, and 544.63 ± 147.49 ng hr/ml, respectively. These results showed that after 18 consecutive days of oral administration of altrenogest, plasma concentrations of altrenogest had a certain degree of fluctuation, without significant accumulations.  相似文献   

猪流行性腹泻IgA、IgG抗体与PEDV抗原相关性研究     

朱海侠  王玉玲  潘海城  曾亮明  傅星源  潘小慧  李枭辰  俞建萍  范凯  黄泽敏  翁燕梅  饶云萍 《福建畜牧兽医》2019,(4):25-29

为了研究仔猪所吮乳汁中IgA抗体水平与乳汁中PEDV感染情况,探讨母猪免疫猪流行性腹泻疫苗后乳汁与血清中IgA、IgG抗体水平的相关性,临床采集多家规模化猪场母猪群乳汁、血清样品以及发病仔猪所吮母猪乳汁样品。采用实验室已经建立的IgA、IgG抗体ELISA方法,检测临床上猪流行性腹泻疫苗免疫后IgA抗体与IgG抗体的相关性,同时采用RT-PCR方法检测发病仔猪所吮乳汁中PEDV感染情况。结果表明,免疫猪流行性腹泻活疫苗后母猪乳汁中IgA抗体水平与血清中IgA、IgG抗体水平呈现很好的正相关性;当发病仔猪所吮母猪乳汁IgA抗体检测结果为阴性时,其PEDV抗原检测结果为阳性;乳汁IgA抗体检测结果趋于阳性样品临界值时,其PEDV抗原检测结果亦为阳性。结论:乳汁中低水平的IgA抗体很难有效地保护仔猪抵御PEDV感染;乳汁中IgA抗体与血清IgA、IgG抗体呈现很好的相关性,且乳汁中IgA抗体水平远远高于血清;通过IgG抗体水平可以间接反映乳汁中的IgA抗体水平,在初乳样品采集难度较大的情况下,可用于间接评估猪流行性腹泻免疫保护水平。  相似文献   

花龄对‘灿烂’兔眼蓝莓花粉原位萌发和花粉管生长的影响     

杨芩  陈磊  张婷渟  李性苑  彭舒  李东平 《北方园艺》2018,(9):57-61

以‘灿烂'兔眼蓝莓为试材,分别进行异花授粉和自花授粉后,于去雄后第0、1、2、3、4、5、6天对去雄的花进行授粉,于授粉后2、6、12、24、48、72、96 h进行取样观察花粉原位萌发和花粉管生长情况,以期阐明花龄对蓝莓花粉萌发和花粉管生长的影响,为杂交育种和花期人工辅助授粉提供科学依据。结果表明:‘灿烂'兔眼蓝莓开花第1~4天,异花授粉后花粉管生长至花柱基部的花柱比率显著高于其它时期,花龄对其异花和自花授粉后花粉原位萌发和花粉管生长有着显著的影响,且‘灿烂'兔眼蓝莓存在自交不亲和的现象,花柱基部为其自交不亲和的关键位置,大蕾期和延迟授粉可以一定程度地克服自交不亲和现象,人工辅助授粉和杂交育种的最佳授粉时间为花后第1~4天。  相似文献   

Sucrose assists selection of high‐quality oocytes in pigs          下载免费PDF全文

Thanh Quang Dang‐Nguyen  Hiep Thi Nguyen  Tamas Somfai  David Wells  Nguyen Thi Men  Nguyen Viet‐Linh  Junko Noguchi  Hiroyuki Kaneko  Kazuhiro Kikuchi  Takashi Nagai 《Animal Science Journal》2018,89(6):880-887

We investigated whether high‐quality in vitro matured (IVM) oocytes can be distinguished from poor ones based on the morphological changes after treatment with hyperosmotic medium containing 0.2 mol/L sucrose in pigs. We hypothesize that IVM oocytes maintaining round shape have higher quality than mis‐shapened oocytes following dehydration. Oocyte quality was verified by determining embryonic developmental competence using in vitro fertilization, nuclear transfer and parthenogenetic activation. In all cases, the round oocytes had greater (p < .05) developmental competence than that of mis‐shapened oocytes in terms of blastocyst rate and total cell number in blastocysts obtained after 6 days of in vitro culture. We also confirm that round aged oocytes are higher in quality than mis‐shapened aged oocytes. In an attempt to find out why high‐quality oocytes maintain a round shape whereas poorer oocytes become mis‐shapened following sucrose treatment, we examined the arrangement of actin microfilaments and microtubules. Abnormal organization of these cytoskeletal components was higher (p < .05) in mis‐shapened oocytes compared to round oocytes after 52 hr of IVM. In conclusion, sucrose treatment helps selection of high‐quality oocytes, including aged oocytes, in pigs. Abnormal cytoskeleton arrangements partly explain for low developmental competence of mis‐shapened oocytes.  相似文献   

原木极边部锯材的研究     

门泽民 《西北林学院学报》1990,5(4):43-47

对原木极边部锯材的研究结果表明:极边部锯材宽度和厚度的精确值为0.247524d×0.031618d,近似值为0.248d×0.03d;出材率3.28％。前者,可作为精确合理设计锯剖方案的依据;后者,可作为编制企业技术经济指标和进行企业经济技术评价的基本参考数据。  相似文献   

多元线性回归中控制问题求解方法的探讨     

孟德顺  李秋元 《西北林学院学报》1995,10(3):76-81

从标准线性回归方程出发,首先确定标准化因变量回归值的控制区间,然后找出一个ｍ元一次不完全方程的解集合,根据回归方程的特定意义,在该解集合中求得满足标准回归方程控制问题的子集合,最后应用标准回归方程与一般回归方程的关系,求出一般线性回归控制问题解的子集合及计算公式,经实例验证符合要求。  相似文献   

大窖调制微储饲料的实用技术     

王承军  张扬 《四川草原》1999,(1):54-57

新疆草地载畜能力下降,饲草不足,而农区有大量各类农作物没有很好的利用。微储饲料技术的应用,在很大程度上缓解了草料不足的问题。在压制微储饲料的生产过程中,我们对原菌液池加以了改进,对葵花头、秆可做微储原料予以肯定。  相似文献   

关于培育渔业中介组织的思考   总被引：1，自引：0，他引：1  

孟全 《现代渔业信息》2009,24(1):18-18

渔业中介组织是应政府转变职能,促进产业优化升级,提高入世之后行业竞争力的需求下发展起来的,其制度变迁路径应走以渔业经济主体自觉自愿为基础与政府推动引导相结合的道路,在培育渔业中介组织的过程中注意政府扶持的方式方法,保持渔业中介组织的独立性,加强法治规范,提高中介组织的整体素质。  相似文献