Th17与Treg细胞免疫失衡在慢性乙型病毒性肝炎发病过程中的调节作用     

王静  高伟  安选  钟卿  胡怀东  胡鹏  任红 《西南大学学报(自然科学版)》2012,34(6):045-049

目的:研究Th17/Treg细胞免疫失衡在慢性乙型病毒性肝炎不同阶段的作用.方法:选取重庆医科大学附属第二医院感染科慢性乙肝患者65例,其中包括7例无症状携带者(AsC),38例慢性乙肝轻、中度患者(CHB-LM),20例慢性重型肝炎患者(CSHB).另有10例健康对照者(HC).用流式细胞术检测外周血中Th17与Treg细胞的频数,并用ELISA方法检测相关细胞因子IL-17,IL-1β,IL-6,IL-21和TGF-β的水平.结果:Th17细胞在CSHB组中的频数明显增加,Treg细胞在AsC组中的比例最高,Th17细胞与Treg细胞之间存在负相关,相关细胞因子(IL-1β,IL-6和IL-21)在CSHB组中的表达均增加,TGF-β因子在各组中无明显变化.结论:Th17与Treg细胞在慢性乙型病毒性肝炎不同疾病阶段存在免疫失衡.在免疫耐受阶段(AsC),Treg细胞发挥主要作用,而在严重炎症活动阶段,Th17细胞发挥主要作用.  相似文献   

多糖水解酶处理烟叶的香气成分鉴定   总被引：1，自引：0，他引：1  

胡璇  魏敏  罗诚浩  刘剑峰  李丹  陈守文 《湖北农业科学》2012,51(10):2098-2100,2103

以α-淀粉酶、纤维素酶和果胶酶组成的多糖水解酶处理烟叶,运用蒸馏萃取法提取烟叶中的香气成分,并结合气相色谱-质谱法(GC-MS)对香气成分进行了定性定量分析.结果表明,多糖水解酶处理的烟叶中水溶性糖含量提高4.29％;烟叶中的香气物质总量提高11.8％;整体口感得到改善,香气的质和量均得到提高,烟气细腻柔和,刺激性、杂气和余味残留有所降低.  相似文献   

An all-silicon passive optical diode     

Fan L  Wang J  Varghese LT  Shen H  Niu B  Xuan Y  Weiner AM  Qi M 《Science (New York, N.Y.)》2012,335(6067):447-450

A passive optical diode effect would be useful for on-chip optical information processing but has been difficult to achieve. Using a method based on optical nonlinearity, we demonstrate a forward-backward transmission ratio of up to 28 decibels within telecommunication wavelengths. Our device, which uses two silicon rings 5 micrometers in radius, is passive yet maintains optical nonreciprocity for a broad range of input power levels, and it performs equally well even if the backward input power is higher than the forward input. The silicon optical diode is ultracompact and is compatible with current complementary metal-oxide semiconductor processing.  相似文献   

筛胸梳爪叩甲的防治技术研究   总被引：4，自引：1，他引：3       下载免费PDF全文

舒金平  滕莹  陈文强  石坚  刘剑  徐天森  王浩杰 《林业科学研究》2012,25(5):620-625

筛胸梳爪叩甲是我国南方地区重要的笋期害虫.研究了林间挖笋除虫、黑光灯诱杀及药剂防治3种方法对筛胸梳爪叩甲的控制效果.结果表明:3种技术措施均能显著降低该虫的危害率和虫口密度,其中药剂防治效果最明显.3 a持续挖笋除虫的防治效果达(22.76±3.90)％;每年单盏黑光灯年平均诱捕量可达(1 143.8±318.7)头,连续6 a的灯光诱杀可将竹笋危害率由防治前的(56.05±2.83)％降至防治后的(39.21±2.83)％;连续施用5％辛硫磷+3％毒死蜱颗粒剂3 a年可将竹笋危害率由防治前的(69.62±5.36)％降至(21.17±7.65)％,防治效果达(70.11±10.99)％.  相似文献   

利用“模拟测算法”计算森林合理年伐量   总被引：2，自引：1，他引：1  

赵晨  储菊香  温雪香  滕轶  李海波 《林业资源管理》2012,(2):65-68

模拟测算法是一种计算森林合理年伐量的新方法,它克服了传统森林合理年伐量计算方法的"主观性",有较强的适应能力,广泛适用于各种森林结构。介绍了模拟测算法的基本原理和计算方法,以期为森林资源管理工作提供参考、借鉴。  相似文献   

Assessment of pregnancy‐associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera     

Van Hanh NGUYEN  Olimpia BARBATO  Xuan Nguyen BUI  Jean‐François BECKERS  Noelita Melo de SOUSA 《Animal Science Journal》2012,83(10):683-689

Pregnancy‐associated glycoproteins (PAG) constitute a large family of glycoproteins found in the outer placental epithelial cell layer of the placenta in Eutherian species. In ruminants, they are noted to be structurally closely related among the different species. This study was designed to determine PAG concentrations in maternal and fetal plasma, allantoic and amniotic fluids in buffalo species. Antisera (AS) generated in rabbits against distinct PAG molecules were used in three radioimmunoassay (RIA)‐PAG systems: RIA‐1 (antiserum raised against bovine PAG67kDa; AS#497), RIA‐2 (antiserum raised against caprine PAG55 + 62 kDa; AS#706) or RIA‐3 (antiserum raised against buffalo PAG; AS#859). Samples were collected at a slaughterhouse (n = 67). PAG concentrations determined by RIA‐2 gave significantly higher results in both allantoic and amniotic fluids (12.7 ± 2.1 ng/mL and 24.0 ± 7.3 ng/mL, respectively). Regarding maternal and fetal plasma, PAG concentrations obtained by RIA‐2 (21.8 ± 2.4 ng/mL and 20.2 ± 2.5 ng/mL, respectively) and RIA‐3 (25.0 ± 2.2 ng/mL and 21.9 ± 3.2 ng/mL, respectively) were higher than those obtained by RIA‐1 (15.5 ± 1.4 ng/mL and 16.1 ± 1.8 ng/mL, respectively). The correlation among the three systems was very high. The study clearly reveals the ability of different PAG‐RIA systems to measure PAG concentration in swamp buffalo samples.  相似文献   

硅和玻璃阳极键合     

刘子建  赵轩  王小安 《山东饲料》2012,(9):104+311

本文叙述了硅与玻璃阳极键合实验过程,对其结合面进行了扫描电镜(SEM)和能谱分析表明离子迁移和阳极氧化是实现键合的关键,其结合机理为电化学反应。  相似文献   

牛SCD基因研究进展   总被引：1，自引：0，他引：1  

陶璇  张健  魏学良 《中国草食动物》2009,29(4)

硬脂酰辅酶A去饱和酶(SCD)是催化主要包括棕榈酰CoA(C16:0)、硬脂酰CoA(C18:0)在内的饱和脂肪酸(SFA)产生单不饱和脂肪酸(MUFA)合成的关键酶,对牛奶、脂肪组织中脂肪酸的组成以及肌肉间脂肪沉积有着重要影响.文章概述了近年来牛SCD基因相关的研究进展.  相似文献   

减少猪场氮污染的方法     

纪志有  丁琳琳  李晓磊  权东升  滕小华 《家畜生态学报》2009,30(4):95-97

规模化猪场废水中含有高浓度的氮,会给环境造成很大的污染,为此国内外学者对去除猪场中的氮污染进行了大量的探索,归纳总结了国内外学者研究的减少猪场氮污染的方法与工艺,以亟为猪场减排污染提供参考.  相似文献   

低氮胁迫下拟南芥低氮耐性突变体lnt1的氮代谢特征     

TENG Yi-Bo  LI Ya-Juan  FANG Ping  LA Gui-Xiao 《土壤圈》2010,20(5):623-632

A growth experiment on agar medium and a hydroponics experiment were carried out to study the nitrogen (N) metabolism of a low-N tolerant mutant (lnt1) of Arabidopsis thaliana under different N levels as compared with the wildtype (WT) Arabidopsis. On the agar medium, no apparent growth differences were observed between the lnt1 and WT plants under a normal N level of 9 mmol L^-1 NO₃^-. However, under a low N level of 0. 18 mmol L^-1 NO₃^-, the growth of the WT plants was greatly retarded, while the lnt1 plants were not affected by low-N stress and showed similar growth with those grown under a normal N level. In the hydroponics experiment, the lnt1 mutant had higher activities of glutamine synthetase (GS) and NADH-dependent glutamate synthase (NADH-GOGAT) in both leaves and roots under N-deficient conditions. Moreover, they accumulated less ammonium (NH₄⁺) but more free amino acids in leaves compared with the WT plants. These observations suggest that better N assimilation might contribute to the low-N tolerant phenotype of the lnt1 mutant.  相似文献