Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells     

Taiko Oda  Jong-Soo Lee  Yuta Sato  Yasuaki Kabe  Satoshi Sakamoto  Hiroshi Handa  Remy E. P. Mangindaan  Michio Namikoshi 《Marine drugs》2009,7(4):589-599

N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14⁺-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis.  相似文献   

Ultrastructural immunolocalization of the otolith water-soluble-matrix in the inner ear of rainbow trout just-hatched fry   总被引：1，自引：0，他引：1  

Yasuaki Takagi 《Fisheries Science》2000,66(1):71-77

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Phylogenetic analysis and PCR detection of Clostridium chauvoei, Clostridium haemolyticum, Clostridium novyi types A and B, and Clostridium septicum based on the flagellin gene     

Sasaki Y  Kojima A  Aoki H  Ogikubo Y  Takikawa N  Tamura Y 《Veterinary microbiology》2002,86(3):257-267

The flagellin genes (fliC) of Clostridium chauvoei, Clostridium haemolyticum, Clostridium novyi types A and B, and Clostridium septicum were analysed by PCR amplification and DNA sequencing. The five Clostridium species have at least two copies of the flagellin gene (fliC) arranged in tandem on the chromosome. The deduced N- and C-terminal aminoacid sequences of the flagellin proteins (FliCs) of these clostridia are well conserved but their central region aminoacid sequences are not. Phylogenic analysis based on the N-terminal aminoacid sequence of the FliC protein revealed that these clostridia, which belong to Clostridium 16S rDNA phylogenic cluster I (), are more closely related to Bacillus subtilis than to Clostridium difficile, which belongs to the cluster XI. Moreover, a multiplex polymerase reaction (PCR) system based on the fliC sequence was developed to rapidly identify C. chauvoei, C. haemolyticum, C. novyi types A and B, and C. septicum. PCR of each Clostridium amplified a species-specific band. The multiplex PCR system may be useful for rapid identification of pathogenic clostridia.  相似文献   

Induction of centrum defects in amberjack Seriola dumerili by exposure of embryos to hypoxia     

Yoshifumi Sawada  Manabu Hattori  Masahide Iteya  Yasuaki Takagi  Kazuhiro Ura  Manabu Seoka  Keitaro Kato  Michio Kurata  Hirofumi Mitatake  Shigekazu Katayama  Hidemi Kumai 《Fisheries Science》2006,72(2):364-372

ABSTRACT:   Artificially hatched Seriola species have the problem of malformation, mainly in their vertebrae, head, and mouth parts. To clarify the cause of vertebral malformation, the effects of hypoxia during embryogenesis on the induction of centrum defects was investigated in artificially hatched amberjack Seriola dumerili . Firstly, 7-somite stage embryos were exposed to waters of 0, 12.5, 25, 50, and 100% dissolved oxygen (DO) for 0.5, 1, 2, 3, 4, and 5 h to confirm the effective dose (DO concentration and duration of exposure) of hypoxia that induces somitic disturbances in newly hatched larvae. Exposure of embryos to 12.5% DO concentration for longer than 0.5 h induced somitic disturbances. Following this result, centrum defects in juveniles were investigated by an induction experiment with embryos exposed to 12.5% DO for 2 h at the gastrula, 1- or 2-somite, 10-somite, 15-somite, or heart beating stage. This experiment revealed that centrum defects were induced only during somitogenesis, and somitic disturbances were the premonitory symptom of centrum defects. These results indicate hypoxia during somitogenesis as a possible cause of centrum defects in amberjack.  相似文献   

Isolation and identification of indigestible pyroglutamyl peptides in an enzymatic hydrolysate of wheat gluten prepared on an industrial scale     

Higaki-Sato N  Sato K  Esumi Y  Okumura T  Yoshikawa H  Tanaka-Kuwajima C  Kurata A  Kotaru M  Kawabata M  Nakamura Y  Ohtsuki K 《Journal of agricultural and food chemistry》2003,51(1):8-13

An enzymatic hydrolysate of wheat gluten was further digested in vitro with porcine pepsin and pancreatin to obtain an indigestible peptide. Indigestible pyroglutamyl peptide was isolated from the digest by strong cation-exchange, size-exclusion, and reversed-phase chromatographies. The pyroglutamyl peptide was digested with pyroglutamate aminopeptidase, and the digest was reacted with phenyl isothiocyanate. The resultant phenylthiocarbamyl (PTC) peptides were purified by reversed-phase HPLC by using binary gradient elution with ammonium acetate buffer, pH 6.0, and acetonitrile. The PTC peptides were analyzed with an automatic peptide sequencer on the basis of the Edman degradation method with a modified program. Some pyroglutamyl peptides were also analyzed by fast-atom bombardment ionization mass spectrometry without the pyroglutamate amino peptidase digestion. Consequently, pyroGlu-Asn-Pro-Gln, pyroGlu-Gln-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gly-Gln-Gly-Gln, pyroGlu-Gln, pyroGlu-Gln-Pro, pyroGlu-Ile-Pro-Gln, pyroGlu-Ile-Pro, pyroGlu-Gln-Pro-Leu, pyroGlu-Gln-Phe-Pro-Gln, pyroGlu-Ser-Phe-Pro-Gln, pyroGlu-Phe-Pro-Gln, and pyroGlu-Gln-Pro-Pro-Phe-Ser were identified.  相似文献   

Scale regeneration and calcification in goldfish <Emphasis Type="Italic">Carassius auratus</Emphasis>: quantitative and morphological processes     

Yasuharu Ohira  Motohiro Shimizu  Kazuhiro Ura  Yasuaki Takagi 《Fisheries Science》2007,73(1):46-54

The time-course changes in calcium and phosphorus contents, dry weight, and area during scale regeneration in the goldfish, Carassius auratus L. were quantified. Histological observations were then conducted to understand the mutual relationship between the quantitative and morphological processes of scale regeneration. The quantitative study revealed that regenerating scales grow most rapidly in area during the first 5 days of regeneration. The gradual decrease in the area growth rate coupled with the continuous linear weight growth over the period of 5–28 days suggests a shift in growth priority from area growth to the apposition, of the basal plate. Calcium and phosphorus deposition proceeded almost linearly during scale regeneration. Calcification of the bony layer preceded that of the basal plate and, after 14 days of regeneration, calcification of the basal plate started and gradually progressed. On day 28, recovery of calcium and phosphorus contents in the regenerating scales were approximatley 72% of ontogenetic scales, which is lower than the rates of area and weight regeneration (104 and 85%, respectively). Late initiation and slow progress of calcification in the basal plate is suggested to be responsible for the slow regeneration in calcium and phosphorus contents.  相似文献   

Atmospheric Concentrations of Sulfur Dioxide and Nitrogen Dioxide in China and Korea Measured by Using the Improved Passive Sampling Method     

Munehiro Warashina  Masanobu Tanaka  Yoshio Tsujino  Tuguo Mizoguchi  Siro Hatakeyama  Yasuaki Maeda 《Water, air, and soil pollution》2001,130(1-4):1505-1510

The results of ambient sulfur dioxide (SO₂) and nitrogen dioxide (NO₂) concentrations measured in ten cities of China and Korea by the improved passive samplers are reported. The property of this sampler is the utilization for the long-term exposure to the high level of SO₂ and NO₂. In this method, the conversion coefficients from the analytical data to the ambient concentrations were obtained from the comparison with the direct concentrations through the automatic analyzers for SO₂ and for NO₂, respectively. The interesting monthly variations were observed in the ambient SO₂ and NO₂ concentrations measured by this passive sampler method, which seems to give important information to the formation of acid rain in these countries.  相似文献   

Isolation and characterization of an attenuated strain of an Orthotospovirus,melon yellow spot virus     

Shimomoto  Yoshifumi  Ishikawa  Koichi  Okada  Tomoyuki  Oki  Tomoka  Hayashi  Kazusa  Yano  Kazutaka  Morita  Yasuaki  Takeuchi  Shigeharu 《Journal of General Plant Pathology》2021,87(5):300-306

Journal of General Plant Pathology - An attenuated mutant strain of melon yellow spot virus (MYSV), an Orthotospovirus, designated as SA08-8, was obtained from a pathogenic isolate, C95S, via high...  相似文献   

Identification,cDNA cloning,and expression analysis of dermatopontin in the goldfish Carassius auratus     

Norihiko Komatsu  Nobuhiro Ogawa  Kurin Iimura  Kazuhiro Ura  Yasuaki Takagi 《Fisheries Science》2014,80(6):1249-1256

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Perchloric acid treatment and use of chromogenic substrate in the Limulus test: Application to veterinary diagnosis     

Yasuaki Shimada  Tsuneo Kume  Kiyoshi Tabuchi 《Veterinary microbiology》1984,10(1):33-42

The Limulus test was carried out for endotoxin determination using a synthetic chromogenic substrate after perchloric acid(PCA) treatment to remove inhibiting substances in the blood.PCA treatment completely removed non-specific amidolytic activity and other factors which interfered with the Limulus test. The recovery of added endotoxin was about 100% irrespective of animal species.Endotoxin levels in PCA-treated blood plasma of healthy domestic animals were invariably below 1 pg ml^?1.Many of the cows with gangrenous mastitis, from which Gram-negative rods were isolated, showed high endotoxin levels ranging from 22.8 to 138.0 pg ml^?1. Endotoxin was also detected in the blood of cows affected with salmonellosis, colibacillosis, Haemophilus somnus infection and urethritis associated with Proteus.  相似文献