Cosmocephalus obvelatus (Creplin, 1825) (Nematoda: Acuariidae) collected from the esophagus of rockhopper penguin, Eudyptes crestatus     

H Azuma  M Okamoto  M Ohbayashi  Y Nishine  T Mukai 《The Japanese journal of veterinary research》1988,36(1):73-77

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Evaluation of genetic diversity in Japanese Brown cattle population by pedigree analysis     

T. Honda  T. Fujii  T. Nomura  & F. Mukai 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(3):172-179

The Japanese Brown is the second most common domestic beef breed in Japan. However, nowadays this breed is facing reduction in numbers because of pressure from a profitable domestic breed. This breed is uniformly characterized by its brown coat colour, but is comprised of two isolated sub‐breeds, Kumamoto and Kouchi, each possessing a different gene pool. Pedigree analyses were carried out for the two sub‐breeds using the pedigree records of animals born from 1970 to 2000. The effective population size has been found to be consistently reducing during the last three decades in both sub‐breeds. The current effective sizes were estimated to be 25.5 and 6.0 for the Kumamoto and Kouchi sub‐breeds, respectively. The estimate of the effective number of founders (N_ef) in the Kumamoto sub‐breed decreased from 152.1 to 74.4; that of non‐founders (N_enf), from 41.7 to 5.3; and that of founder genome equivalents (N_ge), from 32.7 to 4.9. The corresponding changes in the Kouchi sub‐breed were from 108.2 to 79.4, 16.2 to 4.1, and 14.1 to 3.9. Increasing differences between the two genetic diversity indices in the sub‐breeds indicate that the greater part of the reduction of genetic diversity can be attributed to genetic drift that accumulated in the non‐founder generations. A comparison with published estimates for several cattle breeds suggests the extremely limited genetic diversity of Japanese Brown. In addition to the avoidance of further reduction of genetic diversity, it will be important to counteract the process of breed decline by establishing a production system to efficiently utilize the unique characteristics of this breed and by developing links between the breed and products with market value.  相似文献   

The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with Mycobacterium avium subsp. paratuberculosis     

Yamato SAJIKI  Satoru KONNAI  Reiko NAGATA  Satoko KAWAJI  Hayato NAKAMURA  Sotaro FUJISAWA  Tomohiro OKAGAWA  Naoya MAEKAWA  Yukinari KATO  Yasuhiko SUZUKI  Shiro MURATA  Yasuyuki MORI  Kazuhiko OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):162

Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne’s disease.  相似文献   

Simultaneous measurement of swimming speed and tail beat activity of free-swimming rainbow trout Oncorhynchus mykiss using an acceleration data-logger   总被引：2，自引：0，他引：2  

Ryo  KAWABE  Takafumi  KAWANO  Norihiko  NAKANO  Nariharu  YAMASHITA  Tomonori  HIRAISHI  Yasuhiko  NAITO 《Fisheries Science》2003,69(5):959-965

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Epidermoid cyst of the ileum in a miniature dachshund dog     

Shunsuke Shimamura  Risa Kainuma  Ken Kimura  Yasuhiko Okamura  Saori Kobayashi  Masaaki Katayama  Reeko Sato  Jun Yasuda 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(4):366-368

A 13-year-old castrated male miniature dachshund dog was presented with chronic vomiting and diarrhea. Contrast radiography and surgical exploration revealed an ileal cyst, which was excised. A diagnosis of epidermoid cyst was made from a combination of the clinical findings and histological examination of the surgical specimen.  相似文献   

Osteoinductivity of gelatin/β-tricalcium phosphate sponges loaded with different concentrations of mesenchymal stem cells and bone morphogenetic protein-2 in an equine bone defect model     

Jong-pil Seo  Nao Tsuzuki  Shingo Haneda  Kazutaka Yamada  Hidefumi Furuoka  Yasuhiko Tabata  Naoki Sasaki 《Veterinary research communications》2014,38(1):73-80

Fracture is one of the most life-threatening injuries in horses. Fracture repair is often associated with unsatisfactory outcomes and is associated with a high incidence of complications. This study aimed to evaluate the osteogenic effects of gelatin/β-tricalcium phosphate (GT) sponges loaded with different concentrations/ratios of mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2) in an equine bone defect model. Seven thoroughbred horses were used in this study. Eight bone defects were created in the third metatarsal bones of each horse. Then, eight treatments, namely control, GT, GT/M-5, GT/M-6, GT/M-5/B-1, GT/M-5/B-3, GT/M-6/B-1, and GT/M-6/B-3 were applied to the eight different sites in a randomized manner (M-5: 2?×?10⁵ MSCs; M-6: 2?×?10⁶ MSCs; B-1: 1 μg of BMP-2; B-3: 3 μg of BMP-2). Repair of bone defects was assessed by radiography, quantitative computed tomography (QCT), and histopathological evaluation. Radiographic scores and CT values were significantly lower in the control group than in the other groups, while they were significantly higher in the GT/M-5/B-3 and GT/M-6/B-3 groups than in the other groups. The amount of mature compact bone filling the defects was greater in the GT/M-5/B-3 and GT/M-6/B-3 groups than in the other groups. The present study demonstrated that the GT sponge loaded with MSCs and BMP-2 promoted bone regeneration in an equine bone defect model. The GT/MSC/BMP-2 described here may be useful for treating horses with bone injuries.  相似文献   

Preliminary Study of Interaction of Clarithromycin with Tacrolimus in Cats     

Masaaki KATAYAMA  Taku USHIO  Shunsuke SHIMAMURA  Yasuhiko OKAMURA  Yuji UZUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1527-1529

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Effect of cross-sectional dimensions on bow and surface checking of sugi (Cryptomeria japonica) boxed-heart square timber dried by conventional kiln drying     

Kana Yamashita  Yasuhiko Hirakawa  Shuetsu Saito  Hiroshi Nakatani  Motoyoshi Ikeda  Masamitsu Ohta 《Journal of Wood Science》2014,60(1):1-11

The effect of cross-sectional dimensions on bow and surface checking were investigated, using the boxed-heart square timber of two sugi cultivars with dimensions 80, 120, and 140 mm and length 1.9 m taken at two different heights above the ground. The smaller cross-sectional timber tended to have larger bow, less surface checking, and larger dimensional shrinkage. However, the drying defects were different between the cultivars and sampling heights, depending on the shrinkage properties of the juvenile wood and the heartwood proportion in the core part of the stem. The bow was larger in the smaller cross-sectional timber in which the longitudinal shrinkage was large in the juvenile wood. Surface checking was more prominent in larger cross-sectional timber containing sapwood in its outer part, which suggested the surface checking was induced by drying stress, owing to large moisture gradients between the heartwood and sapwood. The cross-dimensional shrinkage of the timber was larger in timber with larger tangential shrinkage.  相似文献   

Detection of hybrids between masu salmon <Emphasis Type="Italic">Oncorhynchus masou masou</Emphasis> and amago salmon <Emphasis Type="Italic">O. m. ishikawae</Emphasis> occurred in the Jinzu River using a random amplified polymorphic DNA technique   总被引：1，自引：0，他引：1  

Yuji?YamazakiEmail author  Nariko?Shimada  Yasuhiko?Tago 《Fisheries Science》2005,71(2):320-326

Identification of masu salmon Oncorhynchus masou masou and amago salmon O. m. ishikawae was accomplished using a random amplified polymorphic DNA (RAPD) technique. Of 80 primers screened, three primers amplified subspecies-specific fragments (OPA11-1095 ^*, OPB5-1618^* and OPD5-2038 ^*). Based on fragment patterns, 33 hybrids were detected among 150 upstream-migrating individuals in the Jinzu River, central Japan, a long-time habitat of masu salmon, to which amago salmon were recently introduced. All of the individuals examined in the 2000–2002 cohorts were identified as F ₁ and F _x as well as pure masu and amago forms. These results indicated continuing hybridization, the genetic pollution of the native masu salmon population by amago salmon possibly being a serious problem in the Jinzu River. Both standard length and body weight in F ₁ hybrids tended to be less than in pure masu salmon. However, F _x hybrids showed similar body sizes to masu salmon.  相似文献