Expression of nitric oxide synthase isoforms in the testes of pigs   总被引：1，自引：0，他引：1  

Kim HC  Byun JS  Lee TK  Jeong CW  Ahn M  Shin T 《Anatomia, histologia, embryologia》2007,36(2):135-138

This study examined the expression of three isoforms of nitric oxide synthase (NOS) in the testes of pigs. Immunohistochemical studies demonstrated the presence of nNOS, eNOS and iNOS in interstitial cells, primary spermatocytes and spermatids. Positive immunoreactions for eNOS and iNOS were detected in peritubular myoid cells. Some vascular endothelial cells were positive for nNOS and eNOS. The expression of nitrotyrosine was detected in interstitial cells. In addition, the histochemical study revealed that all the interstitial cells were stained positively for NADPH-diaphorase, although some spermatids and vascular endothelial cells displayed moderate enzymatic activity. These findings suggest that three isoforms of NOS are expressed in the testis of pig and that they play important roles in the biology of interstitial cells that produce testosterone, as well as in spermatogenesis in the seminiferous tubules.  相似文献   

Pharmacokinetics and mammary residual depletion of erythromycin in healthy lactating ewes     

Goudah A  Sher Shah S  Shin HC  Shim JH  Abd El-Aty AM 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2007,54(10):607-611

The aim of this investigation was to examine the pharmacokinetics and mammary excretion of erythromycin administered to lactating ewes (n = 6) by the intravenous (i.v.), intramuscular (i.m.) and subcutaneous (s.c.) routes at a dosage of 10 mg/kg. Blood and milk samples were collected at pre-determined times, and a microbiological assay method was used to measure erythromycin concentrations in serum and milk. The concentration-time data were analysed by compartmental and non-compartmental kinetic methods. The serum concentration-time data of erythromycin were fit to a two-compartment model after i.v. administration and a one-compartment model with first-order absorption after i.m. and s.c. administration. The elimination half-life (t(1/2beta)) was 4.502 +/- 1.487 h after i.v. administration, 4.874 +/- 0.296 h after i.m. administration and 6.536 +/- 0.151 h after s.c. administration. The clearance value (Cl tot) after i.v. dosing was 1.292 +/- 0.121 l/h/kg. After i.m. and s.c. administration, observed peak erthyromycin concentrations (Cmax) of 0.918 +/- 0.092 microg/ml and 0.787 +/- 0.010 microg/ml were achieved at 0.75 and 1.0 h (Tmax) respectively. The bioavailability obtained after i.m. and s.c. administration was 91.178 +/- 10.232% and 104.573 +/- 9.028% respectively. Erythromycin penetration from blood to milk was quick for all the routes of administration, and the high AUC milk/AUC serum (1.186, 1.057 and 1.108) and Cmax-milk/Cmax-serum ratios reached following i.v., i.m. and s.c. administration, respectively, indicated an extensive penetration of erythromycin into the milk.  相似文献   

海涂农田林网防护效益研究   总被引：2，自引：0，他引：2  

洪昌端  沈辛作  叶根法  张荣锋  施茂荣  朱亚宣 《浙江林业科技》1999,(6)

对上虞市海涂实验农场农田林网防护效益的测定,结果表明通风结构审林带防风效能达到３１．０％左右;在八九月份高温干旱季节,林网内增加相对湿度约１．８％。林带背风而树高２～３倍地区为林带增产作用的显著范围,棉花产量比对照区增产１３．０％～３８．１％,除林缘等胁地外,受林带有利影响测试区棉花产量平均增产８．４％。  相似文献   

Investigation of Kudoa yasunagai (Hsieh & Chen, 1984) infection kinetics in Seriola lalandi (Valenciennes, 1833) by quantitative PCR          下载免费PDF全文

S P Shin  S Shirakashi 《Journal of fish diseases》2017,40(2):287-291

  相似文献   

Induced ovulation of yellow catfish (Pelteobagrus fulvidraco) using a combination of a gonadotrop‐releasing hormone analogue and domperidone     

Youji Wang  Menghong Hu  Siu Gin Cheung  Paul K. S. Shin  Limin Song  Weimin Wang 《Aquaculture Research》2010,41(8):1243-1249

The effects of an intraperitoneal hormone injection of gonadotropin‐releasing hormone agonist (D‐Ala⁶, Pro⁹‐NEt GnRHa) alone or in combination with a dopamine antagonist, domperidone (DOM), on ovulation induction in yellow catfish Pelteobagrus fulvidraco were tested. The hormone treatments were as follows: 6 mg kg⁻¹ body weight (BW) of carp pituitary extract as a positive control, GnRHa 10, 20, 40 and 80 μg kg⁻¹ BW and a combination of GnRHa and DOM as follows: 10 μg+5 mg, 20 μg+10 mg, 40 μg+20 mg and 80 μg+40 mg kg⁻¹ BW. Physiological saline (0.7% NaCl) was used as a negative control. Significant differences in the ovulation ratio, latency period and ovulation index (OI) were observed among treatments (P<0.05). The combination of GnRHa and DOM at doses of 40 μg+20 mg kg⁻¹ BW had higher values of the ovulation ratio and OI, and a shorter latency period compared with other treatments. The highest OI in GnRHa treatments was only 56.67%, suggesting a dopaminergic tone on gonadotropin secretion in this fish at the pre‐ovulatory stage. Therefore, ovulation can be successfully induced in yellow catfish with 40 μg kg⁻¹ GnRHa+20 mg kg⁻¹ DOM without affecting the egg quality.  相似文献   

The importance of riparian subtropical lucidophyllous forest to odonate conservation     

Masaru Sakai  Shin‐ichi Suda  Taichi Okeda  Ryosuke Nomura  Izumi Washitani 《水产资源保护:海洋与淡水生态系统》2019,29(5):682-692

Encapsulation of Phytoncide in Nanofibers by Emulsion Electrospinning and their Antimicrobial Assessment     

Jiyoung Shin  Seungsin Lee 《Fibers and Polymers》2018,19(3):627-634

Phytoncides are volatile organic compounds released from trees and plants and are well known for their natural antibacterial activity. In this study, emulsion electrospinning was used to encapsulate phytoncide in the core of nanofibers, with the aim of developing environmentally friendly, functional nanofibers with a sustained release of the encapsulated component. Core/sheath structured phytoncide/poly(vinyl alcohol) nanofibers were successfully prepared by emulsion electrospinning using an ordinary single-nozzle electrospinning setup. An oil-in-water emulsion of an aqueous solution of poly(vinyl alcohol) (as the aqueous phase) and phytoncide (as the oil phase) was used to prepare the core/sheath structured nanofibers. Nanocomposite fibers were electrospun under various spinning conditions and emulsion formulations to find the suitable processing conditions for fabricating nanofibers with core/sheath structures. The resulting nanofibers exhibited a well-aligned core/sheath structure with fiber diameters of 250-350 nm. The release profile of phytoncide from the core of nanofibers over a 21 day period showed that phytoncide was released in a sustained manner over 14 days. The core/sheath structured phytoncide/poly(vinyl alcohol) nanofibers exhibited 99.9 % bacterial reduction against both Staphylococcus aureus and Escherichia coli, indicating that the encapsulated phytoncide in the fiber provided strong antimicrobial effects.  相似文献   

Effect of SiC and ZnO Nanoparticles on UV Absorbance and Heat Transfer of PET Composite Film     

Sung Won Park  Hye Mi Choi  Dong Soo Shin  Tae Hwan Oh 《Fibers and Polymers》2018,19(1):188-194

Zinc oxide (ZnO) and silicon carbide (SiC) nanoparticles were compounded with polyethylene terephthalate (PET) by using a twin screw extruder, and their effect on the UV absorption and heat transfer in PET/ZnO and PET/SiC composite films was investigated. The presence of ZnO and SiC in the PET matrix was verified by X-ray diffraction. The UV absorbance of both PET/ZnO and PET/SiC composite films increased with increasing particle content. The UV absorbance of PET/SiC was higher than that of PET/ZnO under the same particle content. A thermal imaging camera was used to analyze the heat transfer in PET/ZnO and PET/SiC composite films after heating them to 40 °C. As the content of ZnO and SiC particles increased, the temperature decreased more rapidly. PET/SiC showed faster cooling than PET/ZnO because of the higher thermal conductivity of SiC compared to that of ZnO. Response temperature surfaces for PET/ZnO and PET/SiC were obtained by using an exponential decay function and a second order equation, and the elapsed times to cool to room temperature were estimated.  相似文献   

Evaluation of future climate change impact on snow hydrology for a mountainous watershed of South Korea using SLURP model and NOAA AVHRR images     

Hyung Jin Shin  Minji Park  Seong Joon Kim 《Paddy and Water Environment》2016,14(1):145-158

This study is to evaluate the future potential climate impact on snow hydrology using SLURP model for a 6661.0 km² mountainous watershed of South Korea. For the model test, the NOAA AVHRR images were analyzed to prepare snow-related data of the model. Snow cover areas were extracted using channels 1, 3, and 4, and the snow depth was spatially interpolated using snowfall data of 11 ground meteorological stations. With the snowmelt parameters (snow cover area, snow water equivalent, and snow depth), the model was calibrated for 2 sets (2002–2003, 2004–2005), and verified for 2 sets (1997–1998 and 2001–2002) using the calibrated parameters. The average Nash–Sutcliffe efficiencies during the full year period (December to November) and snowmelt period (December to April) were 0.60 and 0.66, respectively. The future climate data of CCCma CGCM2 SRES A2 and B2 scenarios were adjusted and downscaled using change factor method. By the future impact of climate change, the annual dam inflows were projected to change maximum ?29.3 and ?30.4 % for 2090s A2 scenario and 2030s for B2 scenario, respectively. The future dam inflow increased in winter season (December to February) up to 222.0 %, while other periods decreased up to 54.8 %. The future snowmelt increased in December and January by the future temperature increase of 3.9 °C in minimum. The future snowmelt for the 2 months affected the dam inflows during the winter season.  相似文献   

Modification of SWAT auto-calibration for accurate flow estimation at all flow regimes     

Hyunwoo Kang  Jongpil Moon  Yongchul Shin  Jichul Ryu  Dong Hyuk Kum  Chunhwa Jang  Joongdae Choi  Dong Soo Kong  Kyoung Jae Lim 《Paddy and Water Environment》2016,14(4):499-508

To secure accuracy in the Soil and Water Assessment Tool (SWAT) simulation for various hydrology and water quality studies, calibration and validation should be performed. When calibrating and validating the SWAT model with measured data, the Nash–Sutcliffe efficiency (NSE) is widely used, and is also used as a goal function of auto-calibration in the current SWAT model (SWAT ver. 2009). However, the NSE value has been known to be influenced by high values within a given dataset, at the cost of the accuracy in estimated lower flow values. Furthermore, the NSE is unable to consider direct runoff and baseflow separately. In this study, the existing SWAT auto-calibration was modified with direct runoff separation and flow clustering calibration, and current and modified SWAT auto-calibration were applied to the Soyanggang-dam watershed in South Korea. As a result, the NSE values for total streamflow, high flow, and low flow groups in direct runoff, and baseflow estimated through modified SWAT auto-calibration were 0.84, 0.34, 0.09, and 0.90, respectively. The NSE values of current SWAT auto-calibration were 0.83, 0.47, ?0.14, and 0.90, respectively. As shown in this study, the modified SWAT auto-calibration shows better calibration results than current SWAT auto-calibration. With these capabilities, the SWAT-estimated flow matched the measured flow data well for the entire flow regime. The modified SWAT auto-calibration module developed in this study will provide a very efficient tool for the accurate simulation of hydrology, sediment transport, and water quality with no additional input datasets.  相似文献