Cytoarchitectural differences of myoepithelial cells among goat major salivary glands     

Yaser Hosny Elewa  Mohammad Hafez Bareedy  Ahmed Awad Abu Al Atta  Osamu Ichii  Saori Otsuka  Tomonori Kanazawa  Shin-Hyo Lee  Yoshiharu Hashimoto  Yasuhiro Kon 《Veterinary research communications》2010,34(6):557-567

Previously, the distribution of myoepithelial cells (mecs) in the salivary glands was studied by both immunohistochemistry, and transmission electron microscopy; however, little was elucidated concerning their morphological features, especially in goats. This study was performed to investigate the correlation between the cytoarchitecture of the mecs in goat major salivary glands (parotid, mandibular, and sublingual glands) and the nature of the saliva secretion. The cytoarchitectural features of the mecs were examined by scanning and transmission electron microscopy as well as immunohistochemically. The secretory endpieces in the parotid gland are of the pure serous type, but in both the mandibular and sublingual glands they are of the mixed type. In all studied glands, the intercalated ducts were covered by mecs which, unlike the large stellate cells that surrounded the secretory endpieces, were spindle-shaped with few cytoplasmic processes. Interestingly, the mecs were found to bulge on the basal surfaces of the serous acini and intercalated ducts in all glands and to be in close contact to the seromucous tubules surface in the mandibular and sublingual glands forming a continuous network around it. In conclusion, the differences in the degree of development of the mecs as well as the number of their cytoplasmic processes may be correlated with the nature of the secretion and the number of the secretory granules. Thus these observations may have some relevance in the diagnosis of atrophy and pathogenic conditions of these glands.  相似文献   

奶牛饲喂不同精粗料比TMR对排泄粪尿和氮磷的影响     

孙德成  赵智力  魏曼琳  包丽华  冈本全弘 《家畜生态》2011,(4):85-88

将18头荷斯坦产奶牛随机分为试验6组,每组3头,试验Ⅰ～Ⅵ组奶牛依次饲喂不同精粗料比（精料：粗料）的TMR1（20∶80）、TMR2（30∶70）、TMR3（40∶60）、TMR4（50∶50）、TMR5（60∶40）、TMR6（70∶30）的日粮,探讨不同精粗料比TMR对奶牛粪尿、N、P的排泄量和CH4释放量的影响。结果,饲喂TMR4与饲喂TMR1和TMR6的奶牛相比,每头奶牛每年CH4释放量减少15.20Kg和89.84Kg;粪便排泄量减少1.37t和1.41t;N降低3.71kg和17.50kg;P减少2.59kg和4.07kg,比TMR1FCM提高10.54%（P〈0.05）;乳脂率增加9.67%（P〈0.05）;乳糖增加7.26%（P〈0.05）,能减少奶牛对土壤水源及空气的污染,提高奶牛的生态效益和经济效益。  相似文献   

Effects of whey protein hydrolysate on growth promotion and immunomodulation in mouse pups in artificial rearing system     

Shiro Takeda  Akiko Harauma  Mariko Okamoto  Hirofumi Enomoto  Toshiji Kudo  Takehito Suzuki  Wataru Mizunoya  Toru Moriguchi 《Animal Science Journal》2020,91(1)

This study aimed to investigate the effects of whey protein hydrolysate (WPH) on the growth and immunity of mouse pups in artificial rearing (AR) system. Mouse pups were reared in the AR system with artificial milk including 5% WPH (AR with WPH) or not (AR without WPH), and the remaining pups were reared by their mother (dam) for 14 days after birth. The body weight change and body weight gain rates in the AR with WPH group were significantly higher than those observed in the AR without WPH group and similar to those in the dam group. Moreover the feed and protein efficiencies in the AR with WPH group were significantly higher than those of the AR without WPH group. In addition, the supplement of WPH in the AR system was shown to significantly elevate the number of CD3⁺CD8⁺, B220⁺CD19⁺, IA/IE⁺CD11c⁺, and CD11b⁺ in the thymocyte and/or splenocyte, and the thymus weight. Furthermore, MALDI‐TOF/MS analysis identified the amino acid sequences corresponding to some peptides, and indicated that VRTPEVDDE had the highest relative intensity among the peptides from tested WPH. Therefore, WPH would be required to not only promote growth, but also exert immunomodulatory activities in mouse pups in AR system.  相似文献   

Cardiac lesions in Duchenne muscular dystrophy model rats with out-of-frame Dmd gene mutation mediated by CRISPR/Cas9 system     

Mao Miyamoto  Ryota Tochinai  Shin-ich Sekizawa  Takanori Shiga  Kazuyuki Uchida  Yoshiharu Tsuru  Masayoshi Kuwahara 《Journal of toxicologic pathology》2020,33(4):227

  相似文献   

Photodynamic detection of a canine glioblastoma using 5-aminolevulinic acid     

M. Yamashita  T. Osaki  Y. Sunden  K. Takahashi  M. Ishizuka  T. Tanaka  L. Li  Y. Okamoto 《The Journal of small animal practice》2020,61(8):516-519

Photodynamic detection using 5-aminolevulinic acid has been used to identify the surgical margins during resection of human primary brain tumours. Although there are some reports on its use in malignant tumours in veterinary medicine, it has never been used for primary brain tumours. Here we describe a canine glioblastoma that was detected at autopsy with protoporphyrin IX fluorescence induced by orally administered 5-aminolevulinic acid. The fluorescence was strongest towards the centre of the lesion and was absent in normal brain tissue. Moreover, the fluorescence findings were consistent with MRI and histopathological findings. Our findings suggest that photodynamic detection using 5-aminolevulinic acid might be useful for intraoperative fluorescence-guided resection of malignant gliomas in dogs.  相似文献   

Computed tomographic and radiographic morphometric study of cardiac and coelomic dimensions in captive blue-fronted Amazon parrots (Amazona aestiva,Linnaeus, 1758) with varying body condition scores     

Gisele J. dos Santos  Jeana P. da Silva  Alícia G. Hippólito  Bárbara S. Ferro  Elton Luís R. Oliveira  Priscylla Tatiana C. G. Okamoto  Maria Lucia G. Lourenço  Vania Maria de Vasconcelos Machado  Sheila C. Rahal  Carlos R. Teixeira  Alessandra Melchert 《Anatomia, histologia, embryologia》2020,49(2):299-306

This study aimed to assess radiographic and tomographic cardiac parameters, including width and length of the heart, and the ratio of heart width to coelom width of blue-fronted Amazon parrots (Amazona aestiva) with varying body condition scores. Thirty-five captive birds were included in the study and were allocated into one of three groups according to their respective body condition score: lean, ideal and obese. No differences were observed among the groups with regard to radiographic and tomographic measurements. Computed tomography enabled better identification of the structures of the cardiovascular system without interference from the overlying structures of the celomatic cavity observed in radiographic images. However, radiographic examinations should still be considered the standard screening method to identify cardiac alterations, such as increased or reduced organ dimensions. Standardizing the techniques and measurements performed in this study may serve as a basis for further research in the field.  相似文献   

Growth responses of cereal crops to organic nitrogen in the field     

Miwa Okamoto  Toshihiro Watanabe  Noriharu Ae 《Soil Science and Plant Nutrition》2013,59(3):445-452

Eleven rice varieties differing in grain size were grown under controlled environmental conditions during the grain-filling period. The grain weight of upper grains in a panicle was examined at successive stages of growth during the grain-filling period. The effect of temperature on the rate and the duration of the period of grain-filling was determined using Khao Lo, a large-grain variety, and Bom Dia, a small-grain variety. Both the grain-filling rate and duration of the period of grain-filling differed among rice varieties and were positively and significantly correlated with the grain size. The duration of the grain-filling period from flowering to the time when almost maximum grain weight was attained ranged from 12 days at 32/24°C in Bom Dia to 36 days at 20/12°C in Khao Lo. The grain-filling rate was low in small-grain varieties, and generally increased with increasing grain size.

By lowering the temperature, the grain-filling rate decreased, the duration of the grain-filling period increased but the grain weight was almost constant.

Weight per grain was closely correlated with hull size. No relationship was found between weight per grain and nitrogen percentage of brown rice.  相似文献   

Investigations on Cambodian soils     

Hideaki Saeki  Masao Okamoto  Junzo Azuma  Hirofumi Inoue  Masahiro Takiuchi  Hiroshi Tarumi 《Soil Science and Plant Nutrition》2013,59(1):16-22

Basic information on the nature of soils, their potentialities, and their limitations is necessary to evaluate Cambodian agriculture properly, and to develop a program for increasing food and other crops. Suitable information has, however, been limited, especially as there is not to be found in any report much data relating to soils of different origins over the whole country. There is, needless to say, some information on confined areas of Cambodia.  相似文献   

Effects of potassium nutrition on the glycolysis and the krebs cycle in taro plants     

Saburo Okamoto 《Soil Science and Plant Nutrition》2013,59(5):143-150

The previous works (1, 2) with the bleeding sap from taro plants showed that a potassium deficiency altered the amplitude and the period of the diurnal periodicity in exudation process with the roots, and suggested that potassium might be an essential element for an intensive exchange of amino acids between living xylem tissues and xylem sap. The other works (3,4) with excised roots from sweet potato plants grown in a seven days solution culture showed that the relatively K-deficient roots had higher respiratory activities than the normal, especially In the root tip, and accumulated concomitantly higher levels of reducing sugars and amino nitrogen. Recently, however, an increasing number of works (5, 6) has been devoted exclusively to elucidating the relationship between respiratory activity in plant tissues and organic acid metabolism under a relative K-deficiency. In this work the effects of potassium nutrition on the glycolysis and the Krebs cycle in the roots and the leaves of taro plants are investigated in an attempt to compare these effects in a longer term culture of taro plants with those in a seven days culture of sweet potato plants.  相似文献   

Development of a new high-throughput and small-size method for measuring sediment oxygen demand in lakes     

Shimotori  Koichi  Yamamoto  Haruki  Imai  Akio  Kohzu  Ayato  Okamoto  Takahiro 《Journal of Soils and Sediments》2021,21(2):1222-1231

Journal of Soils and Sediments - Sediment oxygen demand (SOD) measurement currently requires a long preparation time and bulky experimental equipment, which represent major obstacles to conducting...  相似文献