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41.
ABSTRACT Previous work suggested that the efe gene encoding the ethylene-forming enzyme was present in the plasmids of three pathovars of Pseudomonas syringae including glycinea, phaseolicola (kudzu strains), and cannabina. However, no direct evidence to support this assumption had been presented. In the current study, we isolated the conjugative plasmid harboring the efe gene (ethylene plasmid) designated pETH2 from P. syringae pv. glycinea MAFF301683. pETH2 was detected by Southern blot hybridization using the efe probe, marked with the transposon mini-Tn5-Km1, and transferred into P. syringae Ni27(n), which does not produce ethylene. The transconjugant Ni27(n) (pETH2) produced ethylene at a level similar to pv. glycinea MAFF301683. In addition, the plasmid designated pCOR2, which encodes coronatine biosynthesis genes, was detected in the same strain. Although the molecular size of the plasmid pCOR2 was not easily distinguishable from pETH2, pCOR2 transferred independently into Ni27(n) and the transconjugants produced coronatine. These findings suggested that the efe gene has been horizontally dispersed among pathovars of P. syringae by plasmid-mediated conjugation in nature. 相似文献
42.
M. Nagai M. Sato H. Nagano H. Pang X. Kong T. Murakami T. Ozawa H. Akashi 《Veterinary microbiology》1998,60(2-4):271-276
Cytopathogenic and non-cytopathogenic bovine viral diarrhea viruses (BVDVs) were isolated from cattle with mucosal disease or persistent infection in Japan. These isolates were compared for antigenic properties by cross-neutralization tests with Japanese reference strains of BVDV belonging to classical type 1. Significantly low cross-reactivity to reference strains was noted, indicating the viruses to possibly represent a new serotype in Japan. Thus, to determine the genotype of the isolates, nucleotide sequences of the 5′ untranslated region were determined and compared with those of previously reported BVDV 1 and 2. The isolates were clearly shown to belong to BVDV 2, not to BVDV 1. 相似文献
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Jie Liu Rumi Fujita Michiko Sato Kuniyoshi Shimizu Fumiko Konishi Kiyoshi Noda Shoichiro Kumamoto Chie Ueda Hisatoshi Tajiri Shuhei Kaneko Yoshitaro Suimi Ryuichiro Kondo 《Journal of Wood Science》2005,51(2):189-192
The inhibitory effects of 102 methanol extracts of 40 mycelia, 9 culture fluids, and 53 fruiting bodies of 40 strains of Ganoderma lucidum on 5-reductase were investigated. The methanol extract of the fruiting body of each strain was found to show the strongest 5-reductase inhibitory activity among the extracts tested. 相似文献
48.
Self-bonding is the main factor of the performance expression of binderless boards, and therefore its clarification is considered
to be an important issue. For this purpose, a series of chemical analyses were conducted on kenaf core binderless boards and
their chemical changes during the hot-pressing process are discussed in this article. First of all, binderless boards were
prepared from kenaf core powder at different pressing temperatures (without steam-explosion process) and were used for chemical
analyses after they were reduced into powders and extracted with methanol. To investigate their chemical changes, lignin,
holocellulose, and neutral sugar contents were determined, Fourier transform infrared (FTIR) spectra were recorded, and the
nitrobenzene oxidation procedure was applied. As a result, it was found that parts of lignin and hemicelullose were decomposed
during the hot-pressing process; however, the contribution of the resulting fractions to selfbonding was not observed. In
addition, progress of condensation reactions in lignin and the formation of chemical bonds by low molecular weight conjugated
carbonyl compounds in methanol extractives were observed. Thermal softening of lignin is also suggested to play an important
role in the expression of board performance. 相似文献
49.
Binderless boards were prepared from finely ground powders of kenaf (Hibiscus cannabinus L.) core under varying manufacturing conditions. This research was designed to investigate their mechanical properties and evaluate the various manufacturing conditions: pressing temperature and time, pressing pressure, board density, board thickness, grain size of raw materials, and addition of furfural. The mechanical properties (i.e., modulus of rupture and elasticity, internal bonding strength) of boards increased with increasing board density and met the requirement for 15 type medium-density fiberboard (MDF) by JIS A 5905-1994. Thickness swelling and water absorption of boards exceeded the maximum permitted levels for 15 type MDF and S20 grade hardboard by JIS A 5905-1994, which indicates the low water-resistant property of binderless boards. In contrast to that in usual wood-based materials, internal bonding strength showed significant correlations with other board properties: modulus of rupture and elasticity, thickness swelling, and water absorption. We confirmed experimentally that the best manufacturing conditions proved to be as follows: pressing temperature 180°C, time 10min; pressing pressure 5.3MPa; board thickness 5mm; board density 1.0g/cm3; average grain size 53µm; and powder with no furfural content.Part of this paper was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April 2002 相似文献
50.
A split-root apparatus for examining the effects of hydraulic lift by trees on the water status of neighbouring crops 总被引:1,自引:0,他引:1
We describe a split-root system for examining the effects of hydraulic lift by trees on crop growth. In this system, upper
lateral tree roots were grown in a container set on the ground through which the taproot of the tree could penetrate into
the moist soil below. The container, with a radius of 0.5 m and a height of 0.20 m, consisted of two compartments divided
by a waterproof barrier. A markhamia tree (Markhamia lutea (Benth.) Schumann) and upland rice (Oryza sativa (L.)) plants were planted in one compartment, with only rice plants planted in the other compartment. Irrigation of the container
was ceased at the start of the experiment. The stomatal conductance of the rice plants in the associated side, in which both
trees and rice plants were grown, declined more rapidly during the first drying period than in the rice-only compartment,
suggesting that there was competition for water between the tree and the crop plants. However, during the later drying period,
the rice plants in the associated side were green and viable, while those in the rice-only side became desiccated. Rice roots
were seen intermingling with tree roots, and the soil water content in the associated site tended to be higher than in the
rice-only side. It is likely that hydraulic lift occurred in the associated side and that water that had been transferred
to the surface roots was released into the soil, enabling the rice plants in this compartment to remain viable. This novel
system is useful for examination of the effects of hydraulic lift by trees on the growth of neighbouring crops.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献