Dietary calcium-phosphorus ratios for growing pigs in relation to serum levels and bone development     

N C Nielsen  S Andersen  A Madsen  H P Mortensen 《Acta veterinaria Scandinavica》1971,12(2):202-219

Two experiments comprising a total of 80 Danish Landrace pigs in the period 20–90 kg live-weight were carried out to study the effect of dietary levels and ratios of Ca and P on serum values of Ca, inorg. P, and alkaline phosphatase and on the development of degenerative arthritis and atrophic rhinitis.In both experiments each kg feed (Table 1) was supplemented with 600 i. u. vitamin D₃ and 80 p. p. m. Zn.In the first experiment the Ca:P ratio was 1.2 and the amount of Ca increased from 0.48 % to 1.20 % without influencing daily gain, feed conversion, carcass performance (Table 2) or frequency of atrophic rhinitis (Table 3). The Ca:P ratio in scapula was 2.15. Only when the diet was supplemented with 1.20 % Ga and 1.00 % P the bone quality was histomorphologically adequate (Figs. 1–4).In the second experiment various Ca:P ratios (ranging from 0.1 to 3.0) were compared with the recommended level and ratio of 0.72 % Ga and 0.60 % P. Performance of pigs on the normal level was better than for the other pigs (Table 4). Clinically, various degrees of leg-weakness and -deformation occurred, most pronounced in the high Ca — low P group. Very little lameness and no convulsions were observed. Marked hypocalcemia was not found. Unilateral high Ca intake increased serum Ca significantly, and serum alkaline phosphatase slightly, and decreased serum P (Figs. 5–7). Advanced pericytic osteolysis, poor mineralization and generalized osteodystrophia fibrosa were found in the low Ca groups (Figs. 8–13). The groups showed no differences in frequency of atrophic rhinitis (Table 5), and rickets was not observed.The incidence of degenerative joint lesions was not increased in pigs on the unfavourable Ca:P ratios, and depended apparently more on litters than on diets.  相似文献   

Beef Cattle: Seventh edition. A.L. Neumann. John Wiley and Sons,New York,N.Y., 1977, 883 pp., 125 figures, 263 tables + 5 tables in appendix, £ 12.70/US $ 21.80, ISBN 0-471-63236-8     

B. Bech Andersen 《Livestock Production Science》1979,6(1):107

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Risk factors associated with faecal shedding of verocytotoxin-producing Escherichia coli O157 in eight known-infected Danish dairy herds     

Rugbjerg H  Nielsen EM  Andersen JS 《Preventive veterinary medicine》2003,58(3-4):101-113

A risk-factor study was performed in eight dairy herds found to excrete verocytotoxin-producing Escherichia coli (VTEC) O157 in a former prevalence study. Associations between excretion of VTEC O157 and management factors such as housing and feeding were analysed in a generalised linear mixed model. The animals were stratified in three age groups and sampled four times during 1 year. The risk of excreting VTEC O157 was higher among weaned calves than non-weaned calves. Among the calves aged 1–4 months, the risk was reduced if the calf had suckled colostrum from the mother or if the calf had stayed >2 days with the mother after calving. Calves aged 5–24 months that had been moved within the last 2 weeks had a higher risk, but risk was reduced if fed barley silage. Cows fed grain or molasses had a higher risk of excreting VTEC O157.  相似文献   

The need for built-in validation of surveillance data so that changes in diagnostic performance of post-mortem meat inspection can be detected     

Enøe C  Christensen G  Andersen S  Willeberg P 《Preventive veterinary medicine》2003,57(3):117-125

Time trends in animal-disease surveillance often are evaluated on the basis of crude estimates of apparent prevalence. In addition to possible changes in the true prevalence of the condition, changes in apparent prevalence over time might reflect changes in sensitivity and/or specificity of the diagnostic classification used. To illustrate this, comparative post-mortem meat inspection data from four Danish slaughter plants sampled in 1993-1994 and 1997-1998 were used to obtain latent-class model estimates of the sensitivity and specificity of traditional and extended post-mortem meat inspection of visceral and parietal chronic pleuritis (CP), respectively.True prevalence of CP was estimated for each study period and slaughter plant by latent-class models. Estimated sensitivities of traditional post-mortem meat (TPM) inspection ranged from 28.8 to 61.4% (1993-1994) and 39.2 to 87.3% (1997-1998). An increase in sensitivity with time was seen for all slaughter plants. Estimated sensitivities of extended post-mortem meat (EPM) inspection ranged from 85.7 to 94.8% (1993-1994) and 73.8 to 93.0% (1997-1998). All estimated specificities were >93.3%.The possible association of the estimated true prevalence of CP with time (1993-1994 versus 1997-1998) was investigated with a logistic-regression model with random effects. A slight, but non-significant decrease in the odds of CP from 1994 to 1998 was found (odds ratio=0.9).In this and similar situations, one should consider conducting ongoing double-classification of samples of units followed by statistical estimation of true prevalences, sensitivities and specificities, so that decisions can be based on such estimates rather than on crude apparent prevalences.  相似文献   

Comparisons of sampling procedures and time of sampling for the detection of Salmonella in Danish infected chicken flocks raised in floor systems     

Gradel KO  Andersen J  Madsen M 《Acta veterinaria Scandinavica》2002,43(1):21-30

Bacteriological follow-up samples were taken from 41 chicken (Gallus gallus) flocks in floor systems, where Salmonella enterica (Salmonella) had been detected either directly in bacteriological samples or indirectly by serological samples. Three types of follow-up samples were compared to each other within each flock: 1) 5 pairs of socks, analysed as 5 samples, 2) 2 pairs of socks, analysed as one sample, and 3) 60 faecal samples, analysed as one pooled sample. Agreement between sampling methods was evaluated by the following statistical tests: 'Kappa', 'The adjusted rand', McNemar's test for marginal symmetry, Proportion of agreement P0, P+, P-, and Odds Ratio. The highest agreement was found between the 2 types of sock sampling, while the lowest agreement was found by comparing 60 faecal samples with 5 pairs of socks. Two pairs of socks analysed as one pool appeared to be just as effective in detecting S. enterica as the 60 faecal samples. In broiler flocks, 5 pairs of socks were used both in the routine samples taken at about 3 weeks of age for the establishment of infection of the flock, and as one of the follow-up samples taken shortly before slaughter age, which means that the only notable differences between the 2 sampling rounds were the age of the broilers and of their litter. S. enterica was detected more frequently in samples from broilers about 3 weeks old, than in similar samples taken from broilers a few days prior to slaughter at ca. 33-40 days of age.  相似文献   

Effect of different adjuvants on the immune responses of cattle vaccinated with Mycobacterium tuberculosis culture filtrate proteins   总被引：5，自引：0，他引：5  

Wedlock DN  Keen DL  McCarthy AR  Andersen P  Buddle BM 《Veterinary immunology and immunopathology》2002,86(1-2):79-88

The development of improved vaccines for bovine tuberculosis is urgently required as a cost effective solution for control and eventual eradication of tuberculosis in domestic animals. Studies in small animal models of tuberculosis have shown that vaccination with culture filtrate proteins (CFP), prepared from Mycobacterium tuberculosis or M. bovis, can induce cellular immune responses and confer a level of protection against aerogenic challenge with virulent mycobacteria. As a first step in the development of a mycobacterial CFP vaccine for protection of cattle against bovine tuberculosis, the immune responses of cattle vaccinated with short-term culture filtrate proteins (ST-CFP) from M. tuberculosis and formulated with different adjuvants were compared with those vaccinated with bacille Calmette-Guerin (BCG). The adjuvants included dimethyldioctyldecyl ammonium bromide (DDA), diethylaminoethyl (DEAE)-dextran, and ST-CFP adsorbed onto polystyrene beads. Vaccination with ST-CFP/DEAE-dextran induced high levels of interleukin-2 (IL-2) but low levels of interferon-gamma (IFN-gamma) from whole-blood cultures stimulated with M. tuberculosis ST-CFP in comparison with the strong IFN-gamma and IL-2 responses induced after vaccination with BCG. ST-CFP/DEAE-dextran also induced a strong antigen-specific immunoglobulin antibody response with both immunoglobulin G1 (IgG1) and IgG2 isotypes. Vaccination with ST-CFP/beads induced a weak IgG1-biased antibody response but no IFN-gamma or IL-2 response. DDA did not induce significant immune responses in animals vaccinated with ST-CFP. In comparison to the moderate delayed-type hypersensitivity (DTH) responses induced by vaccination with subcutaneous BCG, none of the ST-CFP vaccines induced a significant DTH response to either M. tuberculosis ST-CFP or bovine purified protein derivative (PPD). While the ST-CFP vaccines used in this study have not induced strong antigen-specific cellular immune responses in cattle comparable to those induced by BCG, they are immunogenic in cattle and it may be possible to overcome this problem by using adjuvants that more effectively promote IFN-gamma responses in this species.  相似文献   

Assessment of defined antigens for the diagnosis of bovine tuberculosis in skin test-reactor cattle   总被引：17，自引：0，他引：17  

Pollock JM  Girvin RM  Lightbody KA  Clements RA  Neill SD  Buddle BM  Andersen P 《The Veterinary record》2000,146(23):659-665

The continued use of purified protein derivative (PPD) tuberculin is considered to be the main factor which limits the specificity of diagnostic tests for bovine tuberculosis (TB). This study evaluated a whole blood interferon-gamma (IFN-gamma) assay and compared the diagnostic potential of PPD with two tuberculosis-specific antigens, ESAT-6 and MPB70. To provide estimates of sensitivity and specificity, responses were measured in 180 skin test-reacting cattle, of which 131 were confirmed as tuberculous, and in 128 cattle from TB-free herds. For the skin test reactors, there was a positive correlation between the IFN-gamma responses to PPD from Mycobacterium bovis (PPDB) and PPD from Mycobacterium avium (PPDA), indicating cross-reactivity between these complex antigens which are the basis of the skin test. In comparisons of the ESAT-6 IFN-gamma test with a PPD IFN-gamma test (using PPDB compared with PPDA), there was a decrease in sensitivity (76.3 per cent vs 89.3 per cent), but a clear increase in specificity (99.2 per cent vs 92.2 per cent). The provision of high specificity, even with lower sensitivity, offers major benefits for testing in areas with a low incidence of TB.  相似文献   

‘Candidatus Phytoplasma australiense’ is the phytoplasma associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases     

Lia W. Liefting  Anna C. Padovan  Karen S. Gibb  Ross E. Beever  Mark T. Andersen  Richard D. Newcomb  David L. Beck  Richard L.S. Forster 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):619-623

Sequence comparisons and phylogenetic analysis of the 16S rRNA genes and the 16S/23S spacer regions of the phytoplasmas associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases revealed minimal nucleotide differences between them resulting in the formation of a monophyletic group. Therefore, along with Australian grapevine yellows, the phytoplasmas associated with Phormium yellow leaf and papaya dieback should also be considered as Candidatus Phytoplasma australiense.  相似文献   

Die BlattrandkäferSitona griseus F. undSitona gressorius F. als Lupinenschädlinge     

K. Th. Andersen 《Journal of pest science》1937,13(7):81-84

Ohne Zusammenfassung Mit 5 Abbildungen.  相似文献   

The genetic system for reaction of field beans (Phaseolus vulgaris L.) to infection by three physiologic races of Colletotrichum lindemuthianum     

F. Cardenas  M. W. Adams  A. Andersen 《Euphytica》1964,13(2):178-186

Genetic resistance to race alpha of C. lindemuthianum in the nine varieties of beans studied can be accounted for by dominant alleles at either of two loci, which are unlinked and which behave as duplicate factor loci in the classical sense. A similar system of loci governs reaction to races beta and gamma, with evidence favoring multiple allelism and dominance of alleles conferring susceptibility in certain instances in the case of beta. In addition, for reaction to races beta and gamma, there are distinct systems of complementary factors, again with multiple allelism at the beta loci. Finally, there is genetic linkage between genes of the duplicate factor set governing reaction to gamma and genes of the duplicate factor set, and complementary factor set conditioning reaction to beta. The genes that are members of the set(s) pertaining to any one race alone are independent of each other in a linkage sense.Paper based on work conducted by the senior author in partial fulfilment of requirements for the Doctor of Philosophy degree at Michigan State University and supported by the Rockefeller Foundation.Formerly Rockefeller Foundation Student in Crop Science, now Director, Instituto Nacional de Investigaciones Agricolas, Veracruz, Mexico; Professor of Crop Science, Michigan State University; and Research Pathologist, Crops Research Division, Agricultural Research Service, USDA.  相似文献