Malignant astrocytoma with binucleated granular cells in a Sprague-Dawley rat     

Pruimboom-Brees IM  Brees DJ  Shen AC  Ibebunjo C 《Veterinary pathology》2004,41(3):287-290

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Technical note: a monoclonal antibody-based immunoassay for determination of ractopamine in swine feeds     

Wang JP  Zhang SX  Shen JZ 《Journal of animal science》2006,84(5):1248-1251

An ELISA was developed for routine screening of ractopamine in swine feeds. Swine feed samples were extracted and purified, and the aqueous portion of the extract was analyzed for ractopamine using ELISA and confirmed by HPLC. For swine complex feeds containing ractopamine at 2.5 to 40 mg/kg, the average recoveries ranged from 73.1 to 86.5% by ELISA and 81.9 to 98.2% by HPLC. For the swine supplement containing ractopamine at 50 to 400 mg/kg, the average recoveries were 105.5 to 111.4% by ELISA and 89.1 to 92.9% by HPLC. The limit of detection was 0.24 microg/g by ELISA and 0.48 microg/g by HPLC, respectively. Results from the swine complex feeds (P = 0.009) and the supplement (P = 0.005) using ELISA and HPLC were not highly correlated. The ELISA was more sensitive and rapid and less expensive than the HPLC method and could be used for ractopamine screening in swine feeds before confirmation and quantification by other methods, such as HPLC.  相似文献   

Application of quantum dot-antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue     

Ding S  Chen J  Jiang H  He J  Shi W  Zhao W  Shen J 《Journal of agricultural and food chemistry》2006,54(17):6139-6142

A new indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method for the detection of sulfamethazine (SM2) in chicken muscle tissue was demonstrated using quantum dots (QDs) as the fluorescence label coupled with secondary antibody. The sensitivity of the cFLISA was compared with that of the HPLC method. The 50% inhibition value (IC50) and the limit of detection (LOD) of the cFLISA were 7.7 and 1.0 ng/mL, respectively. When SM2 was spiked at levels of 50, 100, and 200 ng/g, recoveries ranged from 80.6% to 117.4%, with a coefficient of variation of 6.9-9.6%. In the incurred sample analysis, the amounts of SM2 quantified by cFLISA were similar to the results obtained by the HPLC method. This study shows that cFLISA could be used as a screening method in practice.  相似文献   

Ultrafast vibrational dynamics at water interfaces     

McGuire JA  Shen YR 《Science (New York, N.Y.)》2006,313(5795):1945-1948

Time-resolved sum-frequency vibrational spectroscopy permits the study of hitherto neglected ultrafast vibrational dynamics of neat water interfaces. Measurements on interfacial bonded OH stretch modes revealed relaxation behavior on sub-picosecond time scales in close resemblance to that of bulk water. Vibrational excitation is followed by spectral diffusion, vibrational relaxation, and thermalization in the hydrogen-bonding network. Dephasing of the excitation occurs in 相似文献   

Effects of increasing tryptophan intake on growth and physiological changes in nursery pigs     

Shen YB  Voilqué G  Kim JD  Odle J  Kim SW 《Journal of animal science》2012,90(7):2264-2275

Tryptophan (Trp) as a precursor of serotonin (5-hydroxytryptamine, 5-HT) has long been used to extenuate aggressive behavior and control stress of humans as well as several farm animals. This study was conducted to determine the effect of supplemental L-Tryptophan (L-Trp) on growth, cerebral 5-HT concentration, stress hormone concentration, oxidative stress status, and behavior response of pigs under social stress, and also to determine an optimal daily total Trp intake that would benefit nursery pigs under social stress. Seventy two individually housed barrows at 6 wk of age were randomly allotted to 6 treatments with supplementation of 0, 2, 4, 6, 8, or 10 g L-Trp/kg to corn and soybean meal-based feedstuffs. Pigs were fed assigned feedstuffs for 15 d. Body weight was measured on d 0, 5, 10, and 15. Saliva and blood were collected on d 5, 10, and 15. On d 5 and 10, all 12 pigs in each treatment were paired in 6 new pens to record behavior for a 2-d period and returned to original individual pens. On d 15, pigs were euthanized to obtain hypothalamus. During the first 5 d, ADG and G:F increased (linear, P < 0.01) with increasing supplemental L-Trp. During the entire 15 d, ADG and G:F increased (linear, P = 0.01 and P < 0.01, respectively) with increasing supplemental L-Trp. Estimates of the daily total Trp intake based on ADG on d 15 were 10.8 g/d (P < 0.01; R(2) = 0.16) using a 1-slope broken-line analysis. Hypothalamic 5-HT and 5-hydroxyindoleacetic acid increased (linear, P < 0.01 and P = 0.03, respectively) with increasing supplemental L-Trp. Malonedialdehyde in plasma and hypothalamus, as well as salivary cortisol, on d 15 decreased (linear, P = 0.01, P < 0.01, and P < 0.01, respectively) with increasing supplemental L-Trp. Plasma urea nitrogen decreased (linear, P < 0.01) with increasing supplemental L-Trp. Increasing supplemental L-Trp affected pig behaviors during the first 2-d observation period by decreasing (quadratic, P = 0.04) lying, decreasing (linear, P = 0.04) sitting, and increasing (linear, P = 0.02) drinking. Overall, supplementation of L-Trp improved growth performance of 6 wk-old nursery pigs under social stress in association with increasing hypothalamic 5-HT production, reducing stress hormone concentrations, decreasing lipid peroxidation, increasing drinking, and reducing sitting and lying. Increase in BW gain of nursery pigs under social stress was maximal when daily total Trp intake was 10.8 g.  相似文献   

Effect of the diclazuril on Hsp90 in the second-generation merozoites of Eimeria tenella     

Shen X  Wang C  Zhu Q  Li T  Yu L  Zheng W  Fei C  Qiu M  Xue F 《Veterinary parasitology》2012,185(2-4):290-295

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Simulation Analysis and Calculation of Energy Consumption in Green Building Design:A Case Study in Chongqing     

Shen Xiaodong  Hu Wangshe  Xiao Yiming  Li Meng  Xue Ming  Wang Jianqi 《保鲜与加工》2013,(Z1):177-180

It is well known that green energy-saving building should be popularized with great effort. But when it comes to practice, a key stage of building design is how to choose energy-saving technology, products and measures for raising energy-saving levels according to a building's own features. In order to solve the problem, we consider simulate energy-consumption analysis as a tool to synthesize the effect of indicators, such as wall insulation, sunshade, lighting, ventilation etc. Then we compare the result with the national standard to make sure that the energy-saving technology, products and measures really work. As a result, the energy conservation measure can be determined and prominent energy-saving effect can be achieved.  相似文献   

The Application of Controlling Excavation Immersed Tube Technology in Active Pipeline Moving Process     

Liu Yi  Wang Yaozhong  Shen Feijun  Wu Rui  Jin Xin  Li Deming  Cui Di 《保鲜与加工》2013,(Z2):124-128

Taking an example from Zhong-Wu Lang Ping Valley Gas Pipeline Project, we combined the engineering geological of the site area, and learn from experience in similar projects. Then, on this basis, we carried out the design of the immersed work, introduced the applications of the immersed controlled excavation work. The results show that the immersed controlled excavation work achieved the expected results. Practice shows that the controlled excavation construction not only prevent gravity retaining wall concrete construction disturbance and construction personnel devastation, but also improved the depth of pipeline settlement (original design maximum settlement increased to -1.5m from -1.0m.). The program for Zhong-Wu Lang Ping Valley Gas Pipeline engineering success, and can providing guidance for other similar pipeline project.  相似文献   

Starch physicochemical properties and their associations with microsatellite alleles of starch-synthesizing genes in a rice RIL population   总被引：2，自引：0，他引：2  

Bao J  Jin L  Xiao P  Shen S  Sun M  Corke H 《Journal of agricultural and food chemistry》2008,56(5):1589-1594

The physicochemical properties of starch, such as apparent amylose content, gelatinization temperature, and pasting viscosities, determine the eating, cooking, and processing qualities of various products of rice. A recombinant inbred line (RIL) population derived from the reciprocal cross of Lemont (a premium high-quality tropical japonica rice) and Jiayu 293 (a high-yield but low-quality indica rice) was used to test the association of microsatellite markers of starch-synthesizing genes with starch quality parameters. The results confirmed the association of Wx and starch synthase I (SSI) alleles with various starch properties measured in rice flour. However, the starch properties were not associated with the starch branching enzyme 1 (SBE 1) gene alleles.  相似文献   

Nondestructive prediction of total phenolics, flavonoid contents, and antioxidant capacity of rice grain using near-infrared spectroscopy     

Zhang C  Shen Y  Chen J  Xiao P  Bao J 《Journal of agricultural and food chemistry》2008,56(18):8268-8272

Phytochemicals such as phenolics and flavonoids, which are present in rice grains, are associated with reduced risk of developing chronic diseases such as cardiovascular disease, type 2 diabetes, and some cancers. The phenolic and flavonoid compounds in rice grain also contribute to the antioxidant activity. Biofortification of rice grain by conventional breeding is a way to improve nutritional quality so as to combat nutritional deficiency. Since wet chemistry measurement of phenolic and flavonoid contents and antioxidant activity are time-consuming and expensive, a rapid and nondestructive predictive method based on near-infrared spectroscopy (NIRS) would be valuable to measure these nutritional quality parameters. In the present study, calibration models for measurement of phenolic and flavonoid contents and antioxidant capacity were developed using principal component analysis (PCA), partial least-squares regression (PLS), and modified partial least-squares regression (mPLS) methods with the spectra of the dehulled grain (brown rice). The results showed that NIRS could effectively predict the total phenolic contents and antioxidant capacity by PLS and mPLS methods. The standard errors of prediction (SEP) were 47.1 and 45.9 mg gallic acid equivalent (GAE) for phenolic content, and the coefficients of determination ( r (2)) were 0.849 and 0.864 by PLS and mPLS methods, respectively. Both PLS and mPLS methods gave similarly accurate performance for prediction of antioxidant capacity with SEP of 0.28 mM Trolox equivalent antioxidant capacity (TEAC) and r (2) of 0.82. However, the NIRS models were not successful for flavonoid content with the three methods ( r (2) < 0.4). The models reported here are usable for routine screening of a large number of samples in early generation screening in breeding programs.  相似文献