Optimization of Agrobacterium-mediated transformation parameters for Melastomataceae spp. using green fluorescent protein (GFP) as a reporter     

Wilson Thau Lym Yong  Janna Ong Abdullah  Maziah Mahmood 《Scientia Horticulturae》2006

Agrobacterium-mediated transformation for both Melastoma malabathricum and Tibouchina semidecandra were optimized using green fluorescent protein (GFP) as a reporter. The binary vector pCAMBIA1304 harboring the modified green fluorescent protein (mgfp) gene driven by the CaMV 35S promoter was used. Parameters optimized were bacterial strain, bacterial concentration, pre-culture period, co-cultivation period, immersion time, acetosyringone concentration and wounding type. Results obtained were based on the percentage of GFP expression which was observed 3 days post-transformation. Agrobacterium tumefaciens strain LBA4404 and EHA105 at concentration 1 × 10⁷ cfu ml⁻¹ (OD_600nm 0.8) showed the highest virulence on M. malabathricum and T. semidecandra, respectively. Four days of pre-culture and 2 days of co-cultivation were optimum for M. malabathricum transformation, while 3 days of pre-culture and co-cultivation for T. semidecandra. Result also showed that 60 min of immersion and addition of 200 μM acetosyringone gave the highest percentage of positive transformants for both M. malabathricum and T. semidecandra. Mild wounding also significantly increased the efficiency of M. malabathricum transformation.  相似文献   

Detection of African horsesickness (AHS) in recently vaccinated horses with inactivated vaccine in Qatar     

M M Hassanain  A I al-Afaleq  I M Soliman  S K Abdullah 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1990,43(1):33-35

Two 7-year old Arabian racing horses were reported to show typical AHS symptoms in Qatar and died shortly after. The horses had been vaccinated with formol inactivated vaccine approximately 10 days before the onset of the disease. Blood samples from these horses were collected and AHS virus isolated from one sample after intracerebral (i.c.) inoculation into suckling mice. The virus identity was confirmed by complement fixation test (CFT) using the virus antigen and reference type 9 of AHS virus hyperimmune serum. The serotype of the isolated virus was identified by serum neutralization test (SNT) using reference types of AHS virus. Two possibilities of the original source of this infection were suggested. The infection might be due first to the natural endemic occurrence of the virus in the country and secondly, to the presence of residual infectious virus in the inactivated vaccine.  相似文献   

The effect of praziquantel on the hepatic activities of some drug-metabolizing enzymes in rabbits experimentally infected with Schistosomabovis     

H.A. Elsheikh  W.M. Kheir  & A.S. Abdullah 《Journal of veterinary pharmacology and therapeutics》1997,20(5):411-414

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Chemical composition,antioxidant and antibacterial activities of extracts from <Emphasis Type="Italic">Schinus molle</Emphasis> wood branch growing in Egypt     

Mohamed Z. M. Salem  Mohamed Z. Zayed  Hayssam M. Ali  Mamoun S. M. Abd El-Kareem 《Journal of Wood Science》2016,62(6):548-561

In the present work, for the first time, the chemical components of essential oils (EOs) and extracts from wood branch (WB) resulted from the tree pruning wastes of Schinus molle L. grown in Egypt were evaluated for their antioxidant and antibacterial activities. EOs, methanol (ME), dichloromethane (DCME) and water (WE) extracts as antioxidant and antibacterial activities were measured. Total phenolic and flavonoid contents as well as analysis of extracts by gas chromatography–mass spectrometry (GC–MS) were reported. The major components in EOs were α-elemol, β-pinene, and α-phellandrene, in ME were 6-(4-chlorophenyl)-3-cyano-4-(N-benzylpiperazino)-2H-pyran-2-one, and 2-naphthalene methanol, decahydro-α,α,4a-trimethyl-8-methylene, in DCME were 12-methyl-E,E-3,13-octadecadien-1-ol, and 1,2-benzenedicarboxylic acid, dioctyl ester, and in WE were β-eudesmol, and (Z,Z,Z)-9,12,15-octadecatrienoic acid, 2,3-dihydroxypropyl ester. The highest total antioxidant activity was found with EOs (90 ± 1.23 %) and WE (86.30 ± 1.40 %). The lowest IC₅₀ values of 13.11 ± 3.00, and 12.66 ± 2.15 μg/mL were found with WE and EOs, respectively. EOs and WE were observed to have good antibacterial activity against Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Sarcina lutea, Pseudomonas aeruginosa, and Micrococcus luteus. In conclusion, the Schinus molle L. WB EOs and extracts might, indeed, be used as a potential source for pharmaceutical or food industries.  相似文献   

Zur Wirkung von Temperatur und Photoperiode auf die Entwicklung,Ernährung und Eiablage der Raubmilbe Amblyseius swirskii Ath.-Henr. (Acari,Gamasida, Phytoseiidae)     

Dr. Abd el-tawab A. Yousef  A. H. El-keifl  A. M. Metwally 《Journal of pest science》1982,55(7):107-109

Amblyseius swirskii was reared on immature stages ofTetranychus urticae Koch, under 4 temperature and 3 photoperiod regimes. The raise of temperature produced more rapid development and reduced the adult longevity. The mite feeding capacity and fecundity were positively correlated with temperature. At a photoperiod of 16 h the predator developed quicker but it introduced a considerably lower prey consumption and fecundity.  相似文献   

Application of an airborne laser scanner to forest road design with accurate earthwork volumes   总被引：1，自引：0，他引：1  

Kazuhiro Aruga  John Sessions  Abdullah E. Akay 《Journal of Forest Research》2005,10(2):113-123

In this study we developed a forest road design program based on a high-resolution digital elevation model (DEM) from a light detection and ranging (LIDAR) system. After a designer has located the intersection points on a horizontal plane, the model first generates the horizontal alignment and the ground profile. The model precisely generates cross-sections and accurately calculates earthwork volumes using a high-resolution DEM. The model then optimizes the vertical alignment based on construction and maintenance costs using a heuristic technique known as tabu search. As the distance between cross-sections affects the accuracy of earthwork volume calculations, the results were examined by comparing them with the exact earthwork volume calculated by the probabilistic Monte Carlo simulation method. The earthwork volumes calculated by the Pappus-based method were similar to those calculated by the Monte Carlo simulation when the distance between cross-sections was within 10m. The model was applied to a high-resolution DEM from the LIDAR of Capitol Forest in Washington State, USA. The model generated a horizontal alignment, length 827m, composed of five horizontal curves. We examined the number of grade change points. The results indicated that tabu search found the best solution ($61.42/m) with five grade change points. This was composed of two vertical curves that almost followed the ground profile. As the accuracy of a high-resolution DEM from LIDAR increases, the model would become a useful tool for a forest road designer because it eliminates or at least reduces the time-consuming process of road surveys.  相似文献   

Analysis of genomic mutation and immunohistochemistry of platelet‐derived growth factor receptors in canine vascular tumours          下载免费PDF全文

S. Abou Asa  T. Mori  K. Maruo  A. Khater  A. El‐sawak  E. Abd el‐Aziz  T. Yanai  H. Sakai 《Veterinary and comparative oncology》2015,13(3):237-245

We examined whether mutation of the platelet‐derived growth factor receptor protein tyrosine kinase (PDGFR)‐α and PDGFR‐β genes contributes to their overexpression in canine vascular tumours. Genomic sequences of trans‐ or juxtamembrane regions of PDGFR‐α and PDGFR‐β were analysed with immunohistochemical staining and polymerase chain reaction‐direct sequencing using DNA from paraffin‐embedded neoplastic tissues of 27 hemangiosarcomas (HSAs) and 20 hemangiomas (HAs). Immunohistochemically, 75% of the HA cases were positive for PDGFR‐α and almost most of the HA cases were negative for PDGFR‐β. Of the HSA cases, 55.6% were negative for PDGFR‐α and 63% were strongly positive for PDGFR‐β. Among the HA cases, 1 missense mutation was detected in PDGFR‐α exon 18 and 1 in PDGFR‐β exon 17. Two HSA cases had missense mutations in exon 14 and 1 in exon 17 of PDGFR‐β. Thus, genomic mutation of trans‐ or juxtamembrane regions of PDGFRs was not the main mechanism driving the activation of receptors in HSA and HA.  相似文献   

Reducing radioactive cesium transfer from sawdust media to Pleurotus ostreatus fruiting bodies     

Masakazu Hiraide  Masahide Sunagawa  Hitoshi Neda  Nur Humaira’ Lau bt. Abdullah  Satoshi Yoshida 《Journal of Wood Science》2015,61(4):420-430

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Dietary curcumin nanoparticles promoted the performance,antioxidant activity,and humoral immunity,and modulated the hepatic and intestinal histology of Nile tilapia fingerlings     

Abdel-Tawwab  Mohsen  Eissa  El-Sayed Hemdan  Tawfik  Wesam A.  Abd Elnabi  Heba E.  Saadony  Saadea  Bazina  Walaa K.  Ahmed  Ragaa A. 《Fish physiology and biochemistry》2022,48(3):585-601

Fish Physiology and Biochemistry - The current study investigated the effects of dietary curcumin nanoparticles (C-NPs) on the performance, hemato-biochemical profile, digestive enzymes activities,...  相似文献   

Fungal inoculation induces agarwood in young Aquilaria malaccensis trees in the nursery   总被引：2，自引：0，他引：2  

Rozi Mohamed  Phai Lee Jong  Abd Kudus Kamziah 《林业研究》2014,25(1):201-204

Fungi are often used to induce agarwood inAquilariatrees. This study was conducted to evaluate the effect of several fungi on agarwood formation over time in youngAquilaria malaccensis （Lam.） trees. Typical changes in the length and light intensity of the resulting discoloration were observed after three and six month periods following inoculation. Wood samples were observed microscopically and classified into several light intensity groups. The discoloration length was meas-ured longitudinally. The duration after inoculation affected the mean of discoloration length： the 6-month old sample （1.70 cm） had a wider discoloration zone when compared to the 3-month old sample （1.17 cm）. When measuring the discoloration intensity, a positive relationship with time was perceived. Digital images, captured using a camera-equipped microscope, revealed that wood samples collected after six months appeared to be 1.8-times darker than after three months. We concluded that time, not the species of any of the tested fungi, had significant effect on discoloration length and intensity. Gas chromatography/mass spec-trometry （GCMS） analysis of the 6-month old sample yielded some important agarwood compounds such as benzylacetone, anisylacetone, guaiene and palustrol. This demonstrates that the tested fungi have the ability to induce agarwood formation in nurseryA. malaccensis trees.  相似文献