Insights into an undescribed high-elevation lake (6,170 m a.s.l.) on Volcán Llullaillaco: A physical and microbiological view     

Pablo Aran  Pablo Aguilar  Thomas Bowen  Matthew Farson  Joseline Tapia  Lara Vimercati  John L. Darcy  Adam J. Solon  Dorota Porazinska  Steven K. Schmidt  Cristina Dorador 《水产资源保护:海洋与淡水生态系统》2021,31(8):2293-2299

1. High elevation lakes are extreme ecosystems and serve as sentinels of various global changes.
2. An expedition to Volcán Llullaillaco in 1996 discovered an unstudied high-elevation lake (6,170 m a.s.l.) that probably was formed as a result of the past eruptive events or climatic processes such as glacial retreat in the lake basin.
3. This article describes an initial physical characterization of the lake and its microbial communities derived from two sampling expeditions in 2013 and 2016.
4. The microbial community in the lake, with an area between 1.2 and 1.4 ha and a depth of 6.8 m, was dominated by Proteobacteria, Actinobacteria and Haloarchaea. In addition, 26 bacterial isolates were identified within the genera Subtercola, Xylophilus, Rhodanobacter, Mesorhizobium and Pseudomonas.
5. Lago Llullaillaco is one of the highest recorded lakes in the world, and this study highlights the unique microbial diversity of this aquatic ecosystem and the importance of its preservation to understand the complex biological processes under polyextreme conditions.

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Canine influenza virus: cross-species transmission from horses     

Newton R  Cooke A  Elton D  Bryant N  Rash A  Bowman S  Blunden T  Miller J  Hammond TA  Camm I  Day M 《The Veterinary record》2007,161(4):142-143

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Further manipulation by centric misdivision of the 1RS.1BL translocation in wheat   总被引：4，自引：0，他引：4  

Adam J. Lukaszewski 《Euphytica》1997,94(3):257-261

Complete chromosomes 1R and 1B were reconstructed in wheat from the centric wheat-rye translocation 1RS.1BL. Three substitutions: 1R(1A), 1R(1B), 1R(1D), and three new centric translocations: 1RS.1AL, 1RS.1BL, 1RS.1DL were produced from the reconstructed chromosome 1R. Each one of these has the same rye chromosome arm 1RS which was present in the original translocation 1RS.1BL of ‘Kavkaz’ wheat. Reconstructed chromosome 1B and a normal chromosome 1R were used to produce a new 1RS.1BL translocation. This translocation has the long arm from the original 1RS.1BL translocation of ‘Kavkaz’, but a different 1RS arm. The third generation centric translocations were mitotically stable and were normally transmitted to progeny. Misdivision frequency of the reconstructed chromosomes 1R did not change relative to normal 1R, whereas the misdivision frequency of the two reconstructed chromosomes 1B tested was significantly higher relative to normal 1B. These experiments demonstrate that repeated cycles of centric breakage and fusion do not impair the function of centromeres in wheat and rye but may change chromosome's susceptibility to misdivision. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Evaluation of isoflavone aglycon and glycoside distribution in soy plants and soybeans by fast column high-performance liquid chromatography coupled with a diode-array detector     

Klejdus B  Mikelová R  Petrlová J  Potesil D  Adam V  Stiborová M  Hodek P  Vacek J  Kizek R  Kubán V 《Journal of agricultural and food chemistry》2005,53(15):5848-5852

An ultrafast HPLC/UV-vis DAD method working at 254 nm was applied for the determination of isoflavone aglycons and glycosides (genistin, genistein, daidzein, daidzin, glycitin, glycitein, ononin, formononetin, sissotrin, and biochanin A) in roots, stems, leaves, and soy pods of soy plants and in soybeans of five varieties (Korada, Quito, Rita, OAC Erin, and OAC Vison). An Atlantis dC18 ultrafast RP chromatographic column (20 mm x 2.1 mm, 3 microm particle size) was applied for separation of the isoflavone aglycons and glycosides. A flow rate of the mobile phase (0.1% (v/v) acetic acid, pH 3.75-solvent A and methanol-solvent B) was 0.35 mL min(-1), and the column temperature was 36 degrees C. A linear gradient profile from 13 up to 22% B (v/v) from zero to 2.5 min, up to 30% B to 3.21 min, up to 35% B to 4 min, up to 40% B to 4.5 min, up to 50% B to 5.14 min, and followed by negative gradient up to 13% B to 7.71 min was used. The absolute limits of detection per sample injection (5 microL) were the highest for biochanin A (166.2 fmol) and the lowest for genistin (17.0 fmol), respectively. An accelerated solvent extraction (ASE) in combination with sonication was applied for isolation of biologically active compounds. A solid-phase extraction procedure was used to purify the extracts in the case of analysis of soy plants parts. The recoveries of 96-106% were obtained for the different concentrations of the isoflavone aglycons and glycosides and the different matrixes (overall RSDs 2-9%). The highest isoflavone concentrations were found in roots (12.5 microg g(-1) dry weight), while the amounts were about 3-1100 microg g(-1) fresh weight in different varieties of soybeans.  相似文献   

Porous, crystalline, covalent organic frameworks     

Côté AP  Benin AI  Ockwig NW  O'Keeffe M  Matzger AJ  Yaghi OM 《Science (New York, N.Y.)》2005,310(5751):1166-1170

Covalent organic frameworks (COFs) have been designed and successfully synthesized by condensation reactions of phenyl diboronic acid {C6H4[B(OH)2]2} and hexahydroxytriphenylene [C18H6(OH)6]. Powder x-ray diffraction studies of the highly crystalline products (C3H2BO)6.(C9H12)1 (COF-1) and C9H4BO2 (COF-5) revealed expanded porous graphitic layers that are either staggered (COF-1, P6(3)/mmc) or eclipsed (COF-5, P6/mmm). Their crystal structures are entirely held by strong bonds between B, C, and O atoms to form rigid porous architectures with pore sizes ranging from 7 to 27 angstroms. COF-1 and COF-5 exhibit high thermal stability (to temperatures up to 500 degrees to 600 degrees C), permanent porosity, and high surface areas (711 and 1590 square meters per gram, respectively).  相似文献   

Carbon nanotubes provide a charge     

Cohen AE 《Science (New York, N.Y.)》2003,300(5623):1235-6; author reply 1235-6

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Rapid changes in throughput from single motor cortex neurons to muscle activity     

Davidson AG  Chan V  O'Dell R  Schieber MH 《Science (New York, N.Y.)》2007,318(5858):1934-1937

Motor cortex output is capable of considerable reorganization, which involves modulation of excitability within the cortex. Does such reorganization also involve changes beyond the cortex, at the level of throughput from single motor cortex neurons to muscle activity? We examined such throughput during a paradigm that provided incentive for enhancing functional connectivity from motor cortex neurons to muscles. Short-latency throughput from a recorded neuron to muscle activity not present during some behavioral epochs often appeared during others. Such changes in throughput could not always be attributed to a higher neuron firing rate, to more ongoing muscle activity, or to neuronal synchronization, indicating that reorganization of motor cortex output may involve rapid changes in functional connectivity from single motor cortex neurons to alpha-motoneuron pools.  相似文献   

Muscular thin films for building actuators and powering devices     

Feinberg AW  Feigel A  Shevkoplyas SS  Sheehy S  Whitesides GM  Parker KK 《Science (New York, N.Y.)》2007,317(5843):1366-1370

We demonstrate the assembly of biohybrid materials from engineered tissues and synthetic polymer thin films. The constructs were built by culturing neonatal rat ventricular cardiomyocytes on polydimethylsiloxane thin films micropatterned with extracellular matrix proteins to promote spatially ordered, two-dimensional myogenesis. The constructs, termed muscular thin films, adopted functional, three-dimensional conformations when released from a thermally sensitive polymer substrate and were designed to perform biomimetic tasks by varying tissue architecture, thin-film shape, and electrical-pacing protocol. These centimeter-scale constructs perform functions as diverse as gripping, pumping, walking, and swimming with fine spatial and temporal control and generating specific forces as high as 4 millinewtons per square millimeter.  相似文献   

Living landscapes     

Adam DP 《Science (New York, N.Y.)》1994,263(5153):1636-1637

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Phonon dispersions of fcc delta-plutonium-gallium by inelastic x-ray scattering     

Wong J  Krisch M  Farber DL  Occelli F  Schwartz AJ  Chiang TC  Wall M  Boro C  Xu R 《Science (New York, N.Y.)》2003,301(5636):1078-1080

We report an experimental determination of the phonon dispersion curves in a face-centered cubic (fcc) delta-plutonium-0.6 weight % gallium alloy. Several unusual features, including a large elastic anisotropy, a small-shear elastic modulus C', a Kohn-like anomaly in the T1[011] branch, and a pronounced softening of the [111] transverse modes, are found. These features can be related to the phase transitions of plutonium and to strong coupling between the lattice structure and the 5f valence instabilities. Our results also provide a critical test for theoretical treatments of highly correlated 5f electron systems as exemplified by recent dynamical mean field theory calculations for delta-plutonium.  相似文献