Cryopreservation of dead fish spermatozoa several hours after death of Indian major carp, Labeo rohita and its successful utilization in fish production     

P. Routray  A.K. Choudhary  D.K. Verma  P. Swain  S.D. Gupta 《Aquaculture (Amsterdam, Netherlands)》2006,261(4):1204-1211

Cryopreservation of semen collected from dead fishes showed that it is possible to use it for fish production. We humanely killed individuals of the Indian major carp rohu, Labeo rohita and stored at different temperature regimes of 31 °C, 0 °C, − 10 °C and − 30 °C till 8 h. At every one hour interval semen from these fishes were collected by Pasteur pipette and evaluated for sperm yield/kg body weight, motility, pH, spermatocrit (%) and sperm count. The semen having suitable characteristics such as; 70% spermatocrit or above and motility index of 4 or above was cryopreserved by following a specific protocol. The cryopreserved semen of the dead fishes was stored for 7 days and then thawed in a water bath at 37 °C for 50 s. It was found that up to 8 h, spermatozoa of rohu were viable when stored at 0 °C or − 10 °C. Sperm collected after 8 h of fish death and maintained at 0 °C was the best stored condition that showed 30% larval survival. The spermatozoa collected 8 h after fish death was mostly normal as observed under scanning electron microscope and the total length of rohu spermatozoa was 25-30 μm. The hatchlings produced with this cryopreserved semen grew normally and juvenile fishes of rohu could be produced. This study suggests that germ cells such as spermatozoa of dead fishes can be cryopreserved and utilized for restoration of a species. It has the potential use in cryo-conservation of endangered fishes, restoration of animals through fertilization and genetic manipulation studies.  相似文献   

Treatment of wheat straw using tannase and white-rot fungus to improve feed utilization by ruminants     

Shailendra Raghuwanshi  Swati Misra  Rajendra Kumar Saxena 《畜牧与生物技术杂志(英文版)》2014,5(1):13

Background

Current research to enrich cattle feed has primarily focused on treatment using white rot fungi, while there are scarce reports using the enzyme tannase, which is discussed only in reviews or in the form of a hypothesis. In this context, the aim of the present study was to evaluate the effect of tannase on wheat straw (WS) and also the effect of lyophilized tannase at concentrations of 0.1%, 0.2%, and 0.3% (w/w) on WS followed by fermentation with Ganoderma sp. for 10 d and compared in relation to biochemical parameters, crude protein (CP) content, and nutritional value by calculating the C/N ratio in order to improve the nutritional value of cattle feed.

Results

Penicillium charlesii, a tannase-producing microorganism, produced 61.4 IU/mL of tannase in 54 h when 2% (w/v) tannic acid (TA) was initially used as a substrate in medium containing (% w/v) sucrose (1.0), NaNO₃ (1.0), and MgSO₄ (0.08 pH, 5.0) in a 300-L fermentor (working volume 220 L), and concomitantly fed with 1.0% (w/v) TA after 24 h. The yield of partially purified and lyophilized tannase was 5.8 IU/mg. The tannin-free myco-straw at 0.1% (w/w) tannase showed 37.8% (w/w) lignin degradation with only a 20.4% (w/w) decrease in cellulose content and the in vitro feed digestibility was 32.2%. An increase in CP content (up to 1.28-fold) along with a lower C/N ratio of 25.0%, as compared to myco-straw, was obtained.

Conclusions

The use of tannin-free myco-straw has potential to improve the nutritional content of cattle feed. This biological treatment process was safe, eco-friendly, easy to perform, and was less expensive as compared to other treatment methods.  相似文献   

Effect of sub-lethal nitrite on selected haematological parameters in fingerling Catla catla (Hamilton)   总被引：1，自引：0，他引：1  

Pratap Chandra Das  Subanna Ayyappan  Joy Krushna Jena  & Basanta Kumar Das 《Aquaculture Research》2004,35(9):874-880

A sub‐lethal nitrite toxicity trial was conducted using static conditions for a period of 96 h with fingerlings of Catla catla (21.4±3.6 g). Fingerlings were exposed to five concentrations of nitrite, that is, 1, 2, 4, 8 and 10.4 mg L^?1 and a nitrite‐free control to study changes in haematological parameters. Nitrite caused an increase in immature erythrocyte population (7–24%) in lower concentrations (0–4 mg L^?1) at 6 h while they were absent in higher concentrations. The total erythrocyte count was reduced at 6 h followed by an increase at 12 h with further reduction up to 96 h in all concentrations of nitrite. The 96‐h exposure resulted in 21.2–31.8% reduction in erythrocyte population in 1–10.4 mg L^?1 nitrite. The haemoglobin content decreased progressively with increasing nitrite concentrations as well as exposure periods. Total leukocyte count decreased initially at 6 h in all treatments followed by an increase after 12 h, signifying development of a protective response of the body to nitrite stress. Blood glucose decreased initially up to 24 h followed by an increase through 96 h. Serum protein level decreased continuously with increasing exposure period. The study revealed that exposure to nitrite caused changes in almost all the haematological parameters in the fingerlings depending on the concentration as well as exposure period. Nitrite being one of the important inorganic nutrients often recorded at higher levels in intensively cultured ponds, the present study highlights its adverse impact on fish and stressed the need for the management of this nutrient in culture ponds.  相似文献   

Morpho-physio and anatomical characterization of the indigenous Himalayan crabapple: Malus baccata (L.) Borkh. (Rohru) and exotic Malus pumila Mill. for dwarfing traits     

Kumar  Chavlesh  Singh  Sanjay Kumar  Verma  Mahendra Kumar  Pramanick  Kallol Kumar  Srivastav  Manish  Kaur  Sukhdeep  Kumar  Rajeev Ranjan 《Genetic Resources and Crop Evolution》2022,69(2):645-659

Genetic Resources and Crop Evolution - The wild Malus germplasm is considered as a gene reservoir for various biotic and abiotic stresses tolerance/resistance genes, including important novel...  相似文献   

Understanding genetic diversity in blackgram [Vigna mungo (L.) Hepper] collections of Indian National Genebank     

Gayacharan  Tripathi  Kuldeep  Aski  Muraleedhar S.  Singh  Neeta  Kumar  Ashok  Lal  Hanuman 《Genetic Resources and Crop Evolution》2022,69(3):1229-1245

Genetic Resources and Crop Evolution - Blackgram [Vigna mungo (L.) Hepper] is a protein rich pulse crop of Indian origin. The crop is cultivated in diverse range of agro-ecological regions,...  相似文献   

Morphological and molecular characterization of Satputia (Luffa hermaphrodita Singh & Bhandari) revealed substantial diversity for use in breeding programme     

Bhutia  Nangsol D.  Sureja  Amish K.  Munshi  A. D.  Verma  Manjusha  Arya  Lalit  Krishnan  S. Gopala  Gurung  B. 《Genetic Resources and Crop Evolution》2022,69(7):2485-2500

Genetic Resources and Crop Evolution - Luffa hermaphrodita Singh & Bhandari, known as Satputia, a semi-wild taxon originating from L. graveolens is an underutilized vegetable. It has a...  相似文献   

Process Optimization for the Development of Nutritionally Enhanced Nuggets using Ficus geniculata: A Nutritional Approach     

Kumari  Madhu  Kumar  Vikas  Kaur  Ramandeep  Kumar  Satish  Sharma  Rakesh 《Plant foods for human nutrition (Dordrecht, Netherlands)》2022,77(2):241-249

Plant Foods for Human Nutrition - Ficus geniculata (FG) is one of the underutilized fig species in India and throughout the world. However, the different parts of the plant have numerous...  相似文献   

Variability in meteorological droughts as pivotal mechanism for rice production over the middle gangetic plains     

Kumar  Sunny  Sarthi  P. Parth  Barat  Archisman  Sinha  Ashutosh K. 《Paddy and Water Environment》2022,20(4):499-516

The meteorological drought dynamics and its impacts on rice productivity has been evaluated for the Indian Summer Monsoon Rainfall (ISMR) season using the standardized precipitation index (SPI) over the middle Gangetic plains (MGP) of Bihar. The meteorological drought over the ISMR period was found to be a recurring phenomenon coinciding with the rice growing season over Bihar. The rice crop has an intensive water requirement; therefore, it is significantly impacted by the meteorological droughts. In the present study, spatiotemporal characteristics viz. intensity, frequency, and probability of meteorological drought has been assessed along with an investigation for significant trends and detection of regime shift points to identify the impact of drought on rice production. For the purpose, SPI-4 derived from high resolution gridded daily rainfall data (0.25^°?×?0.25^°) from India Meteorological Department (IMD) has been considered to analyse the meteorological drought episodes over agro-climatic zones of Bihar from 1961 to 2019. The regime shifts were determined using the Rodionov test for the drought dynamics and production of rice in Bihar. A moderate to severe drought-prone zone was found over the zone BRZ3B; while zone BRZ2 and BRZ3A showed comparatively a greater number of mild drought events persisting with more than 70% probability of occurrence. An inkling of increasing dependency on groundwater is found, which is in turn governing the rice production regime. The present study shows there is a substantial need for climate resilience and food security policies incorporating the subtle linkage between SPI variability and crop production, especially over rice producing regions of the globe.

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Effect of thyroid hormone depletion on monoamines and expression patterns of catfish GnRH in the air-breathing catfish, Clarias gariepinus     

K. V. Sathya Sai Kumar  I. Swapna  Aparna Dutta Gupta  K. C. Majumdar  B. Senthilkumaran 《Fish physiology and biochemistry》2005,31(2-3):189-192

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Estimation of gut contents of freshwater mussel, Lamellidens marginalis L.     

Rathindra Nath Mandal  Kuldeep Kumar  Utkal Laxmi Mohanty  & Prem Kumar Meher 《Aquaculture Research》2007,38(13):1364-1369

This study examined the food organisms found in the gut of freshwater mussels, Lamellidens marginalis L. A total of 34 taxa of food organisms were recorded, out of which 30 taxa belonged to phytoplankton and four taxa to zooplankton. Both the groups comprised three families each: Cyanophyceae (blue‐green algae), Chlorophycea (green algae) and Bacillariophyceae (diatoms) constituted phytoplankton, whereas Euglenaceae, Chlamydodontidae and Brachionidae belonged to zooplankton. The total number of frequencies of phytoplankton (n=537) were almost nine times more than that of zooplankton (n=60). When blue‐green algae, green algae, diatoms and zooplankton (all the three families together) were tested for significant difference (P<0.05) following Duncan's multiple range test, the result showed only two groups. Blue‐green algae, green algae and zooplankton were not significantly different (P<0.05), forming group A, whereas diatoms were significantly different from others, forming group B. The present study showed that the maximum number of organisms that mussels feed upon belong to green algae, followed by diatoms, blue‐green algae and few taxa of zooplankton.  相似文献