Annual monitoring of growth of red sea bream by multi-stereo-image measurement     

Tanaka  Tatsuya  Ikeda  Ryunosuke  Yuta  Yuta  Tsurukawa  Kanji  Nakamura  Satoshi  Yamaguchi  Takeharu  Komeyama  Kazuyoshi 《Fisheries Science》2019,85(6):1037-1043

Fisheries Science - In aquaculture, periodic measurement of fish body size is required to suitably assess growth progress. The aim of this study is to monitor the growth of free-swimming red sea...  相似文献   

Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen     

Inwoo Bae  Kiyoshi Osatomi  Asami Yoshida  Atsuko Yamaguchi  Katsuyasu Tachibana  Tatsuya Oda  Kenji Hara 《Fisheries Science》2009,75(3):765-770

A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological ionic strength and pH in phosphate buffer and then incubating at 25–37°C. During fibril formation from red stingray collagen, the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated by higher pH or lower NaCl concentration. The T _m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution, 33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse stromal cells at 37°C.  相似文献   

Effect of carpropamid on secondary infection by rice blast fungus     

Yoshio Kurahashi  Shinji Sakawa  Haruhiko Sakuma  Keiko Tanaka  Gerd Haenssler  Isamu Yamaguchi 《Pest management science》1999,55(1):31-37

Carpropamid (WIN™, KTU 3616) provides good control of leaf and panicle blast by ‘one-shot’ nursery-box treatment. It inhibits melanin biosynthesis in appressorial cells of Pyricularia oryzae, making them hyaline. Penetration by infection hyphae from the hyaline appressoria into rice epidermal cells is substantially hindered. In addition, the spread of rice blast spores from primary lesions to the other parts of the plant leading to secondary infection is largely prevented when the plants are treated with carpropamid by spray or water surface application. Secondary infection was simulated in a glass chamber fitted with an ultrasonic humidifier. On treated plants, many blast spores formed in the lesions, but the number of air spora that were dispersed from the lesions decreased significantly. A similar suppression of the spore liberation was observed in vitro when lesions on rice leaf segments, or discs from Pyricularia cultures on oatmeal agar were treated with the chemical. Spores from treated lesions or from the cultures on oatmeal agar amended with the chemical germinated normally and produced well-melanized appressoria on cellophane membranes. In addition, the spores proved to be fully pathogenic towards rice seedlings, producing normal disease symptoms. These results strongly suggest that carpropamid reduces the secondary infection of rice by Pyricularia by specifically hindering spore liberation. © 1999 Society of Chemical Industry  相似文献   

Vitellogenesis and choriogenesis in fishes     

Akihiko Hara  Naoshi Hiramatsu  Toshiaki Fujita 《Fisheries Science》2016,82(2):187-202

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Genetic population structure of the mottled spinefoot <Emphasis Type="Italic">Siganus fuscescens</Emphasis> in Japan     

Naoki Yagishita  Atsuko Yamaguchi 《Fisheries Science》2016,82(2):289-301

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Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines     

Serageldeen Sultan  Nguyen Thi Lan  Toshiki Ueda  Ryoji Yamaguchi  Ken Maeda  Kazushige Kai 《Acta veterinaria Scandinavica》2009,51(1):38

Backgrounds

The aim of this study was to confirm the propagation of various canine distemper viruses (CDV) in hamster cell lines of HmLu and BHK, since only a little is known about the possibility of propagation of CDV in rodent cells irrespective of their epidemiological importance.

Methods

The growth of CDV in hamster cell lines was monitored by titration using Vero.dogSLAMtag (Vero-DST) cells that had been proven to be susceptible to almost all field isolates of CDV, with the preparations of cell-free and cell-associated virus from the cultures infected with recent Asian isolates of CDV (13 strains) and by observing the development of cytopathic effect (CPE) in infected cultures of hamster cell lines.

Results

Eleven of 13 strains grew in HmLu cells, and 12 of 13 strains grew in BHK cells with apparent CPE of cell fusion in the late stage of infection. Two strains and a strain of Asia 1 group could not grow in HmLu cells and BHK cells, respectively.

Conclusion

The present study demonstrates at the first time that hamster cell lines can propagate the majority of Asian field isolates of CDV. The usage of two hamster cell lines suggested to be useful to characterize the field isolates biologically.  相似文献   

Comparison in taste and extractive components of boiled dorsal muscle and broth from half-smooth golden puffer Lagocephalus spadiceus caught in Japan with those of the same fish imported     

Hiroko Yamaguchi  Misako Nakaya  Gen Kaneko  Chie Yoneda  Toshitaka Mochizuki  Katsuya Fukami  Hideki Ushio  Shugo Watabe 《Fisheries Science》2013,79(2):327-334

The taste and extractive components of boiled dorsal muscle and broth prepared from half-smooth golden puffer Lagocephalus spadiceus caught in Japan and from those imported from China were compared. In the sensory test, the first taste, elasticity, and saltiness of boiled muscle from Japanese (domestic) fish were higher than those of the imported fish, whereas the orthonasal fishy smell of the imported fish was higher than that of the domestic fish. The first taste, aftertaste, retronasal fishy smell, sweetness, saltiness, and umami of the broth prepared from dorsal muscle of the domestic fish were higher than those of the imported fish, whereas orthonasal fishy smell and bitterness of the imported fish were higher than those of the domestic fish. Most panelists preferred the overall taste of the domestic fish to that of the imported fish. The concentration of trimethylamine in the trichloroacetic acid extracts from boiled muscle and broth of the imported fish was higher than that of the domestic fish, suggesting that this substance contributes to the orthonasal fishy smell of the imported fish.  相似文献   

First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens     

Hideo Ishii  Junko Tanoue  Michiyo Oshima  Wen-Hsin Chung  Kumiko Nishimura  Junichiro Yamaguchi  Fumihiro Nemoto  Kazuhiro So  Toshitaka Iwama  Hideaki Yoshimatsu  Motoshige Shimizu  Toru Kozawa 《Journal of General Plant Pathology》2008,74(6):409-416

Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences.  相似文献   

Culture method and growth characteristics of marine benthic dinoflagellate Ostreopsis spp. isolated from Japanese coastal waters     

Haruo Yamaguchi  Yuko Tanimoto  Takamichi Yoshimatsu  Shinya Sato  Tomohiro Nishimura  Keita Uehara  Masao Adachi 《Fisheries Science》2012,78(5):993-1000

Blooms of toxic dinoflagellates of the genus Ostreopsis, which is known as a producer of palytoxin (PTX) analogs, may pose a threat to human health in tropical, subtropical, and temperate regions around the world. In the present study, we established a suitable culture method for Ostreopsis spp. isolated from Japanese coastal waters and characterized their growth potential using the method to discuss their bloom dynamics. Each clonal strain of Ostreopsis cf. ovata, Ostreopsis sp.?1, Ostreopsis sp.?5, and Ostreopsis sp.?6 was incubated in 25?×?150?mm test tubes with a flat bottom containing various kinds of medium. Since Ostreopsis spp. strains grew well in IMK and/or f/2 media, we selected these media for cultivation of all the Ostreopsis spp. isolates. Growth rates of O. cf. ovata (0.834?divisions/day), Ostreopsis sp.?1 (0.619?divisions/day), and Ostreopsis sp.?6 (1.04?divisions/day) that produce PTX analogs significantly differed (p?<?0.05) respectively and are clearly higher than those of other reported epiphytic dinoflagellate Gambierdiscus toxicus, Prorocentrum lima, and Coolia monotis cultures, which suggest that these species have ecological advantages to predominate through the algal succession in Japanese coastal waters, resulting in a potential risk to human health in this region.  相似文献   

Comparison of the amount of thiotrophic symbionts in the deep-sea mussel <Emphasis Type="Italic">Bathymodiolus septemdierum</Emphasis> under different sulfide levels using fluorescent in situ hybridization     

Masaru Fujinoki  Tomoko Koito  Suguru Nemoto  Mitsugu Kitada  Yoko Yamaguchi  Susumu Hyodo  Hideki Numanami  Nobuyuki Miyazaki  Koji Inoue 《Fisheries Science》2012,78(1):139-146

Various invertebrates inhabiting hydrothermal vents harbor thiotrophic endosymbionts that provide the host with nutrients and are possibly involved in the detoxification of harmful sulfides. In this study, we first determined the partial 16S rRNA gene sequence of the thiotrophic symbiont of the deep-sea mussel Bathymodiolus septemdierum, a dominant species at hydrothermal vents in the Izu–Ogasawara (Bonin) area. We then designed a new probe, Bsob692, for fluorescent in situ hybridization (FISH) using regions completely conserved among thiotrophic symbionts of all bathymodiolin mussels and established the protocol for FISH to compare the distribution and amount of the symbiont using an image analysis program that is commercially available. We compared fluorescent intensity in the gill of the mussels collected at different sites and found a higher intensity in specimens collected from a site with higher sulfide concentration. We also compared mussels reared in the presence and absence of sulfide and found that the former had a higher fluorescent intensity.  相似文献