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41.
Michishita M Akiyoshi R Suemizu H Nakagawa T Sasaki N Takemitsu H Arai T Takahashi K 《Veterinary journal (London, England : 1997)》2012,193(2):508-513
Increasing evidence suggests that diverse solid tumours arise from a small population of cells known as cancer stem cells or tumour-initiating cells. Cancer stem cells in several solid tumours are enriched for aldehyde dehydrogenase (ALDH) activity. High levels of ALDH activity (ALDH(high)) were detected in four cell lines derived from canine mammary carcinomas. ALDH(high) cells were enriched in a CD44(+)CD24(-) population having self-renewal capacity. Xenotransplantation into immunodeficient mice demonstrated that 1×10(4) ALDH(high) cells were sufficient for tumour formation in all injected mice, whereas 1×10(4) ALDH(low) cells failed to initiate any tumours. ALDH(high)-derived tumours contained both ALDH(+) and ALDH(-) cells, indicating that these cells had cancer stem cell-like properties. 相似文献
42.
Noda K Aoki M Akiyoshi H Asaki H Shimada T Ohashi F 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):727-729
The functions of polymorphonuclear cells (PMN) are the important non-specific defense mechanisms in the immune system. Especially marine mammals are protected by these mechanisms from the aquatic environment with a large variety of microorganisms. Therefore, we examined the PMN functions of bottlenose dolphins in order to obtain the normal ranges and to standardize the techniques. PMNs were isolated by using lymphocyte isolate solution whose density was 1.077; superoxide production was assessed by nitroblue tetrazolium reduction test (NBT) and phagocytosis was tested by using polystyrene latex beads. We showed that the optimal incubation time was 30 min in NBT assay and 12 hr in phagocytosis assay for dolphin PMNs. 相似文献
43.
Toshie ISERI Junichiro SHIMIZU Hideo AKIYOSHI Kayo KUSUDA Akiyoshi HAYASHI Keiichiro MIE Takeshi IZAWA Mitsuru KUWAMURA Jyoji YAMATE Yuka FUJIMOTO Fumihito OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):857-860
A Bengal tiger was presented for evaluation of weakness, ataxia and inappetance. Computed
tomography (CT) and magnetic resonance imaging (MRI) revealed a mass extending from the
T7-8 vertebral body to the left rib and compressing the spinal cord. On CT, the bone
destruction and sequestrum were shown. On MRI, the multilobulated mass appeared hypo- to
isointense in T1-weighted and hyperintense in T2-weighted images. The tiger died after
imaging, most likely from renal failure. Chordoma without metastasis was diagnosed on
necropsy. The imaging characteristics were similar to those found in chordoma in humans.
This report describes the use of CT and MRI in an exotic species. 相似文献
44.
Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development 
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下载免费PDF全文 Akiyoshi Egashira Nobuhiko Yamauchi Md. Rashedul Islam Kazuki Yamagami Asami Tanaka Hikaru Suyama El‐Sharawy Mohamed El‐Sayed Shoji Tabata Takashi Kuramoto 《Animal Science Journal》2016,87(8):1048-1054
This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1‐ and 2‐cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4‐cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2‐ to 4‐cell arrested embryos and reduce the developmental competence to blastocyst. 相似文献
45.
Tanno T Tanaka Y Sugiura T Akiyoshi H Takenaka S Kuwamura M Yamate J Ohashi F Kubo K Tsuyama S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1173-1177
Slit, a secreted protein, functions as a chemorepellent factor in axon guidance and neuronal migration and as an inhibitor in leukocyte chemotaxis. In humans, slit2 protein attracts endothelial cells and promotes tube formation in the tumor angiogenic mechanism. In this study, we cloned a part of the canine slit subfamily and examined the expression of slit subfamily mRNAs in 3 normal canine mammary glands and 11 mammary tumor samples by RT-PCR. The cloned part of the slit gene sequences showed high similarity to those of the human, mouse, and rat. The mRNAs were expressed at low levels in the normal mammary gland. The expression levels of slit1 mRNA were low in both the normal and tumor tissues. In contrast, the expression of slit2 mRNA increased in most of the malignant mammary tumors, and an increase in slit3 mRNA expression was observed in 2 of the malignant mixed tumors. These results suggest that the expression of slit2 plays an important role in tumor angiogenesis in canine mammary gland tumors and that slit2 can be a putative marker for malignancy diagnosis of these tumors. 相似文献
46.
Tanaka T Akiyoshi H Shimazaki H Sato A Inada M Kiyomiya K Shimizu J Cao Z Li Y Hayashi A Sone K Kitai T Ohashi F 《Journal of Feline Medicine and Surgery》2012,14(2):155-157
A 5-month-old male Abyssinian presented with right hemiplegia. Cervical radiographs, haematology and serum biochemistry were normal. A foreign body was identified on computed tomography (CT) and magnetic resonance imaging (MRI). Removal of the foreign body was carried out via a dorsal approach between the occipital bone and the C1 vertebral body using intra-operative CT. No complications were encountered. Thirty days after surgery, the cat was ambulatory. 相似文献
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48.
Akiyoshi EGASHIRA Nobuhiko YAMAUCHI Keiko TANAKA Chihiro MINE Hitomi OTSUBO Masao MURAKAMI Md. Rashedul ISLAM Misako OHTSUKA Naomi YOSHIOKA Takashi KURAMOTO 《The Journal of reproduction and development》2015,61(6):595-600
The presence of multinucleated blastomeres (MNBs) in embryos is associated with poor developmental
competence in assisted reproductive technologies. This phenomenon is observed not only in humans but also in
other animal species. The purpose of the present study was to investigate the characteristics of embryos with
MNBs (MNB embryos) that could be utilized in embryo transfer. The developmental rate of MNB embryos to the
blastocyst stage (50.8%) was significantly lower than that of normal embryos (73.3%) (P < 0.05). The
clinical pregnancy rates of fresh embryo transfer (ET) using day 2 or day 3 embryos were significantly lower
in MNB embryos (5.1%) compared with normal embryos (24.0%) (P < 0.05). In the case of frozen-thawed ET
using a single vitrified/warmed blastocyst, however, the clinical pregnancy rate of MNB embryos was close to
that of normal embryos (59.1% vs. 52.8%). Thus, the findings of the present study suggest
that the frozen-thawed ET of MNB embryos might improve the potential for implantation followed by successful
pregnancy. 相似文献
49.
Fukuhara A Matsuda M Nishizawa M Segawa K Tanaka M Kishimoto K Matsuki Y Murakami M Ichisaka T Murakami H Watanabe E Takagi T Akiyoshi M Ohtsubo T Kihara S Yamashita S Makishima M Funahashi T Yamanaka S Hiramatsu R Matsuzawa Y Shimomura I 《Science (New York, N.Y.)》2005,307(5708):426-430
Fat tissue produces a variety of secreted proteins (adipocytokines) with important roles in metabolism. We isolated a newly identified adipocytokine, visfatin, that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin corresponds to a protein identified previously as pre-B cell colony-enhancing factor (PBEF), a 52-kilodalton cytokine expressed in lymphocytes. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. Mice heterozygous for a targeted mutation in the visfatin gene had modestly higher levels of plasma glucose relative to wild-type littermates. Surprisingly, visfatin binds to and activates the insulin receptor. Further study of visfatin's physiological role may lead to new insights into glucose homeostasis and/or new therapies for metabolic disorders such as diabetes. 相似文献
50.
Fukuoka K Sawabe A Sugimoto T Koga M Okuda H Kitayama T Shirai M Komai K Komemushi S Matsuda K 《Journal of agricultural and food chemistry》2004,52(20):6326-6329
Atherosclerosis is a vascular disorder involving inflammation, a narrowed vascular lumen in the entire tunica intima, and reduced elasticity of the arterial wall. It has been found that Hsp60 from Chlamydia pneumoniae, an obligate bacterial pathogen associated with atheroma lesions, mimics human Hsp60, thereby causing attacks by immune cells on stressed endothelial cells expressing endogenous Hsp60 on their surface. Furthermore, Hsp60 from C. pneumoniae has been shown to promote the growth of vascular smooth muscle cells (VSMCs). To explore probes that can be used for studying signal transduction elicited by the chlamydial Hsp60, we have tested several natural products for their inhibitory actions on the Hsp60-induced proliferation of rat arterial smooth muscle cells. Sesamol, vanillyl alcohol, and trans-ferulic acid exhibited moderate inhibitory actions on the Hsp60-induced cell proliferation; zerumbone, humulene, and caryophylene effectively inhibited it at low concentrations with IC(50) values of 529, 122, and 110 nM, respectively. The results indicated that the 11-membered alicyclic ring is favorable for interactions with receptors involved in the Hsp60-induced VSMC proliferation. 相似文献