A Novel C1q Domain-Containing Protein Isolated from the Mollusk Modiolus kurilensis Recognizing Glycans Enriched with Acidic Galactans and Mannans     

Andrei V. Grinchenko  Alex von Kriegsheim  Nikita A. Shved  Anna E. Egorova  Diana V. Ilyaskina  Tatiana D. Karp  Nikolay V. Goncharov  Irina Y. Petrova  Vadim V. Kumeiko 《Marine drugs》2021,19(12)

C1q domain-containing (C1qDC) proteins are a group of biopolymers involved in immune response as pattern recognition receptors (PRRs) in a lectin-like manner. A new protein MkC1qDC from the hemolymph plasma of Modiolus kurilensis bivalve mollusk widespread in the Northwest Pacific was purified. The isolation procedure included ammonium sulfate precipitation followed by affinity chromatography on pectin-Sepharose. The full-length MkC1qDC sequence was assembled using de novo mass-spectrometry peptide sequencing complemented with N-terminal Edman’s degradation, and included 176 amino acid residues with molecular mass of 19 kDa displaying high homology to bivalve C1qDC proteins. MkC1qDC demonstrated antibacterial properties against Gram-negative and Gram-positive strains. MkC1qDC binds to a number of saccharides in Ca²⁺-dependent manner which characterized by structural meta-similarity in acidic group enrichment of galactose and mannose derivatives incorporated in diversified molecular species of glycans. Alginate, κ-carrageenan, fucoidan, and pectin were found to be highly effective inhibitors of MkC1qDC activity. Yeast mannan, lipopolysaccharide (LPS), peptidoglycan (PGN) and mucin showed an inhibitory effect at concentrations three orders of magnitude greater than for the most effective saccharides. MkC1qDC localized to the mussel hemal system and interstitial compartment. Intriguingly, MkC1qDC was found to suppress proliferation of human adenocarcinoma HeLa cells in a dose-dependent manner, indicating to the biomedical potential of MkC1qDC protein.  相似文献   

Combining ability analysis of earliness and yield of potato (<Emphasis Type="Italic">Solanum tuberosum</Emphasis> L.) genotypes in Uganda     

Prossy Namugga  Julia Sibiya  Rob Melis  Alex Barekye 《Euphytica》2018,214(7):116

Potato (Solanum tuberosum L.) is a major food and cash crop mainly grown by small-scale farmers in the highland regions of Uganda. Changing global weather patterns require varieties that are able to grow within the short rainfall cycles and yield optimally under the prevailing conditions. The objectives of this study were to estimate the combining ability effects for early maturity, yield and yield related traits in potato. Eighteen F¹ families generated from two sets of 12 parents using a North Carolina Design II were evaluated for days to 50% flowering, leaf senescence, yield and yield related traits in two different locations. Both additive and non-additive genetic effects influenced the expression of traits. However, additive genetic effects were predominant over the non-additive for most of the traits. The GCA/SCA ratios were 0.68 and 0.78 for days to 50% flowering and average tuber weight. Broad sense heritability estimates were 0.70 for total tuber weight and 0.78 for days to 50% flowering. The predominance of additive genetic effects imply that, genetic gains can be achieved through different selection methods and traits transferred to the respective progenies. Parents Rwangume, 396,038.107, 395,011.2, NKRK19.17, 393,077.54, Kimuri, and 392,657.8 had desirable GCA effects for the number of days to flowering and yield related traits. Families of Rwangume?×?NKRK19.17, 393,077.54?×?395,011.2, 396,038.107?×?Rwangume and 396,038.107?×?395,011.2 had desirable SCA effects for yield and number of days to 50% flowering. The selected parents and families will be subjected to further clonal evaluation and selection.  相似文献   

Environmental influences on Atlantic bluefin tuna (Thunnus thynnus) catch per unit effort in the southern Gulf of St. Lawrence     

Angelia S. M. Vanderlaan  Alex R. Hanke  Joël Chassé  John D. Neilson 《Fisheries Oceanography》2014,23(1):83-100

The Atlantic bluefin tuna (Thunnus thynnus) population in the western Atlantic supports substantial commercial and recreational fisheries. Despite quota establishment and management under the auspices of the International Commission for the Conservation of Atlantic Tunas, only small increases in population growth have been estimated. In contrast to other western bluefin tuna fisheries indices, contemporary estimates of catch per unit effort (CPUE) in the southern Gulf of St. Lawrence have increased rapidly and are at record highs. This area is characterized by the Cold Intermediate Layer (CIL) that is defined by waters <3°C and located at depths of 30–40 m in September. We investigated the influence of several in situ environmental variables on the bluefin tuna fishery CPUE using delta‐lognormal modelling and relatively extensive and consistent oceanographic survey data, as well as dockside monitoring and mandatory logbook data associated with the fishery. Although there is considerable spatial and temporal variation of water mass characteristics, the amount of available habitat in the southern Gulf of St. Lawrence (assuming a > 3°C thermal ambit) for bluefin tuna has been increasing. The percentage of the water column occupied by the CIL was a significant environmental variable in the standardization of CPUE estimates. There was also a negative relationship between the spatial extents of the CIL and the fishery. Properties of the CIL account for variation in the bluefin tuna CPUE and may be a factor in determining the amount of available feeding habitat for bluefin tuna in the southern Gulf of St. Lawrence.  相似文献   

State-of-the-art review of goat TSE in the European Union, with special emphasis on PRNP genetics and epidemiology     

Gabriele Vaccari  Cynthia H. Panagiotidis  Cristina Acin  Simone Peletto  Francis Barillet  Pierluigi Acutis  Alex Bossers  Jan Langeveld  Lucien van Keulen  Theodoros Sklaviadis  Juan J. Badiola  Olivier Andr��oletti  Martin H. Groschup  Umberto Agrimi  James Foster    Wilfred Goldmann 《Veterinary research》2009,40(5)

Scrapie is a fatal, neurodegenerative disease of sheep and goats. It is also the earliest known member in the family of diseases classified as transmissible spongiform encephalopathies (TSE) or prion diseases, which includes Creutzfeldt-Jakob disease in humans, bovine spongiform encephalopathy (BSE), and chronic wasting disease in cervids. The recent revelation of naturally occurring BSE in a goat has brought the issue of TSE in goats to the attention of the public. In contrast to scrapie, BSE presents a proven risk to humans. The risk of goat BSE, however, is difficult to evaluate, as our knowledge of TSE in goats is limited. Natural caprine scrapie has been discovered throughout Europe, with reported cases generally being greatest in countries with the highest goat populations. As with sheep scrapie, susceptibility and incubation period duration of goat scrapie are most likely controlled by the prion protein (PrP) gene (PRNP). Like the PRNP of sheep, the caprine PRNP shows significantly greater variability than that of cattle and humans. Although PRNP variability in goats differs from that observed in sheep, the two species share several identical alleles. Moreover, while the ARR allele associated with enhancing resistance in sheep is not present in the goat PRNP, there is evidence for the existence of other PrP variants related to resistance. This review presents the current knowledge of the epidemiology of caprine scrapie within the major European goat populations, and compiles the current data on genetic variability of PRNP.  相似文献   

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Alex Dugdale  Nicki Grint  & John Burford 《The Journal of small animal practice》2009,50(7):378-379

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Statistical analysis of the effect of multi-stage hot drawing on the thermal shrinkage and crystallographic order of biodegradable aliphatic-aromatic co-polyester fibres     

Basel Younes  Alex Fotheringham  Robert Mather 《Fibers and Polymers》2011,12(6):778-788

Using statistical experimental design, the effects of multi-stage hot drawing process on thermal shrinkage and crystallographic order of linear aliphatic-aromatic co-polyester fibres were statistically analyzed. Thermal shrinkage and fullwidth half-maximum (FWHM) of an X-ray scattering profile of the fibres have been quantitatively assessed, and the direction of increasing or decreasing the significant process parameters has been characterized. It has been found that the number of drawing stages, drawing temperature and relaxing stage ratio affect the shrinkage and crystallographic order together. Plate temperature and the interaction between drawing stages and drawing temperature or drawing ratio have an effect on thermal shrinkage; Spin finish application and relaxing temperature have an effect on crystallographic order. The data analysis has been characterized with regard to the relationship between the studied factors statistically analyzed and the scientific reasons suggested. Factorial statistical analysis simulates the combination of drawing process parameters and the selected as-spun environmentally friendly biodegradable fibre.  相似文献   

Impact of three silvicultural treatments on growth,light-energy processing,and related needle-level adaptive traits of Pinus strobus from two regions     

John E. Major  Alex MosselerDebby C. Barsi  Britt Corriveau-DupuisMoira Campbell 《Forest Ecology and Management》2009

Our goal was to quantify and compare the impact of three silvicultural treatments (STs) on growth, light-energy processing, and needle-level morphological adaptive traits for eastern white pine (Pinus strobus L.) from large, central Ontario (ON) and small, isolated Newfoundland (NL) populations. The interest in STs is to reduce weevil (Pissodes strobi) incidence; however, there are potential adaptive changes and productivity trade-offs. The light levels for the STs were, on average, 100%, 42.0%, and 20.4% transmittance for the full-sun, and intermediate- and high-shade STs, respectively. After 8 years, overall height growth was 4.10, 3.25, and 1.70 m for full-sun, and intermediate- and high-shade STs, respectively (P < 0.001). Across all STs, ON populations had greater total height (14%), basal diameter (12%), current leader length (25%), and tree volume (49%) than NL populations (all P < 0.001). At low light levels (10 and 25 μmol m⁻² s⁻¹), high-shade ST trees had higher photochemical quenching (qP) and lower chlorophyll fluorescence (F_pc) compared with intermediate-shade and full-sun STs. At 100 μmol m⁻² s⁻¹ and beyond, full-sun ST trees had higher qP and lower F_pc than intermediate- and high-shade STs. Average total chlorophyll concentration (CHL) and content (CHLC), and carotenoid concentration (CAR), increased in response to the intermediate-shade ST but did not respond further, or decreased in the high-shade ST. Region was significant for CHL, CAR, chlorophyll a:b and CHL:CAR ratios and CHLC, with ON greater than NL, but was reversed for CHL:CAR ratio. Tree height and volume showed a curvilinear and linear relationship to light level, respectively. Tree height showed a positive linear relationship to qP, apparent photosynthesis, chlorophyll a:b ratio, and needle N (all P < 0.001). Tree height showed a negative linear relationship to F_pc, CHL:CAR ratio, specific needle area, C:N ratio, and needle area N⁻¹ (all P < 0.001). There were modest trade-offs between weevil protection and productivity in the intermediate ST due to the compensatory physiological and morphological adaptations to the limiting light, however, the trade-off with growth at the high-shade level was severe. For NL, consideration should now be given to the introduction and mixing of seed from local seed sources with more southern mainland seed sources, which would decrease the inbreeding effect and provide wider variation for natural selection for a more fit future population.  相似文献   

Liming as an ameliorator of copper toxicity in black oat (Avena strigosa Schreb.)     

Vítor Gabriel Ambrosini  Daniel José Rosa  Alex Basso  Marcelo Borghezan  Rosete Pescador  Alcione Miotto 《Journal of plant nutrition》2017,40(3):404-416

This study aimed to assess the effect of liming as an ameliorator of copper (Cu) toxicity in black oats (Avena strigosa Schreb.) grown in soil with a sandy texture. The treatments were soil supplementation with increasing doses of lime (0.0, 0.5 and 1.0 g kg^?1) and two of Cu (0 and 50 mg kg^?1). Pre-germinated black oat seeds were sown for 30 days in the soil, subsequently, the plant height, root and shoot dry mass and concentration of total chlorophyll, chlorophylls a and b, carotenoids and total soluble carbohydrates and starch were analyzed. The dose of 50 mg kg^?1 soil Cu caused a toxic effect on black oat plant growth and development, affected the internal distribution of nutrients, decreased the leaf chlorophyll concentration and increased the leaf concentration of total soluble carbohydrates and starch. Increasing lime doses ameliorated the toxic effects of Cu.  相似文献   

Investigating the impact of innate dexterity skills and visuospatial aptitude on the performance of baseline laparoscopic skills in veterinary students          下载免费PDF全文

Mathew R. A. MacCormick  Jessica J. Kilkenny MSc  Meagan Walker BSc  Alex zur Linden DVM  DACVR  Ameet Singh DVM  DVSc  DACVS 《Veterinary surgery : VS》2017,46(8):1175-1186

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Evidence for a collective intelligence factor in the performance of human groups   总被引：2，自引：0，他引：2  

Woolley AW  Chabris CF  Pentland A  Hashmi N  Malone TW 《Science (New York, N.Y.)》2010,330(6004):686-688

Psychologists have repeatedly shown that a single statistical factor--often called "general intelligence"--emerges from the correlations among people's performance on a wide variety of cognitive tasks. But no one has systematically examined whether a similar kind of "collective intelligence" exists for groups of people. In two studies with 699 people, working in groups of two to five, we find converging evidence of a general collective intelligence factor that explains a group's performance on a wide variety of tasks. This "c factor" is not strongly correlated with the average or maximum individual intelligence of group members but is correlated with the average social sensitivity of group members, the equality in distribution of conversational turn-taking, and the proportion of females in the group.  相似文献