排序方式： 共有216条查询结果，搜索用时 0 毫秒
211.
Sima Tavakolinejad Mohsen Khosravi Baratali Mashkani Alireza Ebrahimzadeh Bideskan Nasser Sanjar Mossavi Seyyed Mohammad Reza Parizadeh Daryoush Hamidi Alamdari 《Iranian Biomedical Journal》2014,18(3):151-157
Background: The cultured mesenchymal stem cells (MSC) have been used in many clinical trials; however, there are still some concerns about the cultural conditions. One concern is related to the use of FBS as a widely used xenogeneic supplement in the culture system. Human platelet-rich plasma (hPRP) is a candidate replacement for FBS. In this study, the effect of hPRP on MSC proliferation and osteogenic differentiation has been evaluated. Methods: Human adipose-derived stem cells (hADSC) were expanded. Cells from the third passage were characterized by flow cytometric analysis and used for in vitro experiments. Resazurin and alizarin red stains were used for cell proliferation and osteogenic differentiation assays, respectively. Results: Treatment with hPRP resulted in a statistically significant increase in cell proliferation compare to the negative control group (P<0.001). Cell proliferation in the 15% hPRP group was also significantly higher than that in the 10% hPRP group (P<0.05). Additionally, it caused less osteogenic differentiation of the hADSC compared to the FBS (P<0.001), but in comparison to negative control, it caused acceptable mineralization (P<0.001). Conclusion: These findings indicate that hPRP not only improves the proliferation but also it can be a suitable substitution in osteogenic differentiation for clinical purposes. However, the clinical application value of hPRP still needs more investigation. Key Words: Platelet-Rich Plasma, Adipose tissue, Stem Cells, Cell differentiation, Cell proliferation 相似文献
212.
213.
Najmeh Ahangari Amirhossein Sahebkar Mohsen Azimi-Nezhad Hamideh Ghazizadeh Mohsen Moohebati Mahmoud Ebrahimi Habibollah Esmaeili Gordon A. Ferns Alireza Pasdar Majid Ghayour Mobarhan 《Iranian Biomedical Journal》2021,25(5):374
Background:FH, a hereditary disorder, is caused by pathogenic variants in the LDLR, APOB, and PCSK9 genes. This study has assessed genetic variants in a family, clinically diagnosed with FH. Methods:A family was recruited from MASHAD study in Iran with possible FH based on the Simon Broom criteria. The DNA sample of an affected individual (proband) was analyzed using WES, followed by bioinformatics and segregation analyses. Results:A novel splice site variant (c.345-2A>G) was detected in the LDLRAP1 gene, which was segregated in all affected family members. Moreover, HMGCR rs3846662 g.23092A>G was found to be homozygous (G/G) in the proband, probably leading to reduced response to simvastatin and pravastatin. Conclusion:LDLRAP1 c.345-2A>G could alter the PTB, which acts as an important part of biological pathways related to lipid metabolism. Key Words: Genetic research, LDLRAP1, Hypercholesterolemia, Hydroxymethylglutaryl-CoA Reductase Inhibitors 相似文献
214.
Alireza Reyshari Hamid Mohammadiazarm Takavar Mohammadian Mansour Torfi Mozanzadeh 《Aquaculture Nutrition》2019,25(5):1135-1144
This study was conducted to evaluate the effects of dietary sodium diformate (NaDF) on growth performance, gut microflora, digestive enzyme activities and immune response parameters of Asian sea bass (Lates calcarifer) juveniles. Fish with initial weight of 12.5 ± 0.4 g were fed with five experimental diets contained 0.0 (control), 5, 10.0, 15.0 and 20 g NaDF kg?1 in triplicate for 6 weeks. Fish fed diet containing 5 g NaDF kg?1 had significantly the highest final body weight and feed intake among different treatments. The gut total viable bacterial counts gradually decreased with increasing dietary NaDF level. Specific activity of chymotrypsin improved in fish fed diets administered with NaDF compared to the control group. Fish fed 5 g NaDF kg?1 diet showed the highest serum lysozyme level among different treatments. The serum classical pathway activity of complement showed higher level in fish fed diets contained 5 and 10 g NaDF kg?1 than other groups. According to break‐point regression method analysis, the optimum inclusion of dietary NaDF in L. calcarifer juveniles was estimated between 4.6 and 5.1 g/kg, when specific growth rate and feed conversion ratio were plotted against dietary NaDF levels. 相似文献
215.
Seyed Hossein Hoseinifar Alireza Mirvaghefi Daniel L. Merrifield Bagher Mojazi Amiri Saeed Yelghi Kazem Darvish Bastami 《Fish physiology and biochemistry》2011,37(1):91-96
A study was conducted to investigate the effects of dietary oligofructose (1, 2 and 3%) on the blood profiles of beluga (Huso huso) juveniles (18.77 ± 0.76 g) compared to fish fed an un-supplemented diet. After 7 weeks of feeding on the experimental diets,
haematological parameters, metabolic products (cholesterol, glucose and total protein) and serum enzymes (lactate dehydrogenase,
alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were measured. Compared to the control group
(0% oligofructose), dietary oligofructose had no effect on red blood cell counts (RBC), mean corpuscular volume (MCV), mean
cellular haemoglobin (MCH) or mean cell haemoglobin concentration (MCHC) (P > 0.05). However, haemoglobin (Hb) concentration, leucocyte (WBC) levels and the proportion of lymphocytes were significantly
higher (P > 0.05) in the 2% oligofructose fed fish than in the 3% oligofructose fed fish. Additionally, haematocrit (Hct) values (P = 0.049) and the proportion of lymphocytes (P ≤ 0.01) were significantly higher in the 2% oligofructose group than in the control group. Although serum glucose and total
protein remained unaffected, serum cholesterol was significantly lower in the 2% oligofructose group than in the control and
3% oligofructose group (P < 0.05). The results of the present study showed that oligofructose had no significant effects on serum lactate dehydrogenase,
alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. These results indicate that fish blood profiles
could be affected by prebiotics, which should be taken into account in future studies. 相似文献
216.
Hadi Ghaffari Ahmad Tavakoli Mohammad Faranoush Ali Naderi Seyed Jalal Kiani Alireza Sadeghipour Davod Javanmard Mohammad Farahmand Saied Ghorbani Farnoush Sedaghati Seyed Hamidreza Monavari 《Iranian Biomedical Journal》2021,25(6):426
Background:Glioblastoma multiforme is the most invasive and lethal form of brain cancer with unclear etiology. Our study aimed to investigate the molecular prevalence of HCMV and EBV infections in patients with GBM. Methods:This case-control study was conducted on 42 FFPE brain tumor samples from GBM patients and 42 brain autopsies from subjects without neurological disorders. The presence of EBV and HCMV DNA was determined, using PCR and nested-PCR assays, respectively. Results:HCMV DNA was detected in 3 out of 42 (7.1%) of GBM samples and was absent from the control group (p = 0.07). Importantly, EBV DNA was detected in 9 out of 42 (21.4%) brain tissue specimens of GBM subjects, but again in none of the control group (p = 0.001). Conclusion:Our findings indicate that infection with EBV is associated with GBM. Key Words: Brain tumor, Epstein-Barr virus, Glioblastoma, Human cytomegalovirus 相似文献