Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia     

Nor Zulkiply Amalina  Zulperi Dzarifah  Mohammad Noor Azmai Amal  Mohd Termizi Yusof  Mohd Zamri‐Saad  Nurhidayu Al‐saari  Mami Tanaka  Sayaka Mino  Tomoo Sawabe  Md Yasin Ina‐Salwany 《Aquaculture Research》2019,50(11):3202-3210

Vibrio infections are common among marine fish and lead to serious problems in the aquaculture sector. This study reports a recent occurrence of Vibrio species (spp.) isolated from cultured groupers in Peninsular Malaysia using the gyrB and pyrH genes. A total of 147 Vibrio strains were successfully isolated from 77 (64%) groupers using culture method and subjected to gyrB and pyrH sequencing for species identification and confirmation. Results showed that 89% of Vibrio strains were identified and clustered to six groups of Vibrio spp., while 11% were not clustered to any Vibrio spp. using the gyrB sequences. Meanwhile, by analysis of the pyrH sequences all the 147 Vibrio strains (100%) were successfully identified and clustered into 11 groups of Vibrio spp., including the gyrB non‐identified strains. The pyrH gene provides a better resolution for identification of Vibrio spp. compared with the gyrB gene. Thus, the pyrH gene was more suitable for a rapid determination of Vibrio spp. distribution in Peninsular Malaysia. Using the pyrH gene, our study found higher prevalence of Vibrio vulnificus (33%), V. alginolyticus (24%) and V. parahaemolyticus (22%), followed by V. rotiferianus (5%), V. harveyi (3%), V. tubiashii (2%), V. campbellii (2%), V. ponticus (1%), V. diabolicus (1%), V. owensii (1%) and others Vibrio sp. (7%). Thus, the results of this study revealed that the occurrence of pathogenic vibrios among grouper fish is still high in Malaysian aquaculture. In addition, the pyrH gene was proved as a suitable marker for rapid identification of Vibrio species compared with the gyrB gene.  相似文献   

Phosphorus dynamics and solubilizing microorganisms in acid soils under different land uses of Lesser Himalayas of India   总被引：1，自引：0，他引：1  

Ranjan Paul  Raj Deo Singh  A. K. Patra  D. R. Biswas  Ranjan Bhattacharyya  K. Arunkumar 《Agroforestry Systems》2018,92(2):449-461

Although chemical and some soil physical properties have been studied under different land uses of the Lesser Himalayas of India, very limited information is available on soil biochemical properties. Hence we investigated phosphorus (P) fractions [total P (TP), inorganic P (P_i), organic P (P_o), available P, microbial biomass P (MBP)], enzyme activities [dehydrogenase, phosphatases, phytase], phosphate solubilizing bacteria (PSB) and fungi (PSF), and their correlations of acid soils (0–15 and 15–30 cm depths) under different land uses (viz, organic farming, maize–wheat, apple orchard, undisturbed oak forest and uncultivated land of the Indian Himalayas). All land use systems differed significantly for the P fractions, except TP. The highest values for TP, P_i, available P and MBP were found in soils under oak forest and lowest in uncultivated land. However, P_o content was highest in apple orchard. The organic farming (organic manures field under garden pea-french bean cropping system for > 10 years) maintained highest activities of dehydrogenase, acid phosphatase and alkaline phosphatase. The highest phytase activity and highest numbers of PSB (99 × 10³ g^?1 soil) and PSF (30 × 10³ g^?1 soil) were observed in the rhizosphere soils of oak forest. Significant relationships between soil P fractions and enzyme activities, except alkaline phosphatase, were recorded in surface soil layer. PSB and PSF population were also correlated significantly with P fractions and enzyme activities. This would lead us to understand the level of degradation of P pools due to cultivation over forest system and the suitable management practices needed for soil quality restoration.  相似文献   

Embryonic and larval developments of brackish water catfish,Mystus gulio (Hamilton and Buchanan, 1822) induced with human chorionic gonadotropin and consequent larval rearing          下载免费PDF全文

Prem Kumar  Gouranga Biswas  Tapas Kumar Ghoshal  Muniyandi Kailasam  Koyadan Kizhakedath Vijayan 《Aquaculture Research》2018,49(7):2466-2476

Mystus gulio, the long whiskers catfish, is a popular food fish and potential candidate species for aquaculture in Sundarban area of India and Bangladesh. Recently, catch of this species has declined due to overfishing and various ecological changes. In the present study, mature fish was induced to spawn in captivity through intramuscular injection of human chorionic gonadotropin at the doses of 10 IU/g to female and 5 IU/g to male. Photomicrographs of all developmental stages of live embryo and larvae were documented with the aid of a light microscope. Results demonstrated that morula, blastula, gastrula and neurula and organogenesis ended at 1:30‐, 3:00‐, 5:30‐, 7:30‐ and 17:15‐hour post‐spawning (hps) respectively. Heartbeat and muscular contraction of embryo commenced at 8:30 and 11.15 hps respectively. Hatching of embryo started after an incubation period of 17:30 hour at an ambient temperature of 29 ± 1°C. The newly hatched larvae measured 2.17 ± 0.29 mm in total length with a yolk volume of 0.165 ± 0.03 mm³ started feeding 36 hr after hatching. The present study, on induced breeding and chronological development of M. gulio embryo, will have significant implications on conservation and seed production for aquaculture.  相似文献   

Influence of different microalgal diets on gonadal development of the carpet shell clam <Emphasis Type="Italic">Ruditapes decussatus</Emphasis> broodstock     

Ahmed S. A. Abbas  Eman El-Wazzan  Amal R. Khafage  Abdel-Fattah M. El-Sayed  Fatma A. Abdel Razek 《Aquaculture International》2018,26(5):1297-1309

Studies considering broodstock conditioning of Ruditapes decussatus mostly used flagellates and diatoms for feeding, mainly Isochrysis galbana and Chaetoceros calcitrans, respectively. The present study evaluated the effect of different microalgal diets on gonadic maturation of R. decussatus including three microalgae species (C. calcitrans, Tetraselmis suecica, and Nannochloropsis oculata) which were used to prepare six diets: three monospecific and three bispecific mixed diets at a proportion of 1:1. Clams were daily fed at a ratio of 1% dry weight of algae/live weight of clam and unfed treatment was used as control. Results showed that clams fed C. calcitrans alone or mixed with T. suecica attained earlier full maturity with the highest response to spawning induction (after 34 conditioning days) and largest proportion in the partial spawning phase for both, followed by T. suecica alone after 48 days. In contrast, N. oculata alone or mixed diets demonstrated poor performance for gonadal maturation. Unfed treatment did not show any sign of maturation. The results indicated that T. suecica has high conditioning potential like C. calcitrans and their mixture gave the highest benefit. Therefore, T. suecica can be recommended for R. decussatus broodstock conditioning.  相似文献   

Main effect QTLs associated with arsenic phyto-toxicity tolerance at seedling stage in rice (Oryza sativa L.)     

Md. Abu Syed  K. M. Iftekharuddaula  M. A. Khaleque Mian  Md. Golam Rasul  G. K. M. Mustafizur Rahmam  Golam M. Panaullah  Julie G. Lauren  John M. Duxbury  Partha S. Biswas 《Euphytica》2016,209(3):805-814

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Evaluation of Differential Pore-Volume Distribution (DPVD) Patterns of Porous Clinoptilolite Fractions     

K. Ramesh  K. Sammi Reddy  I. Rashmi  A.K. Biswas 《Communications in Soil Science and Plant Analysis》2016,47(3):369-374

Analysis of differential pore-volume distribution (DPVD) patterns of a commercial Clinoptilolite has been conducted experimentally using an analyzer by measuring the N₂ adsorption isotherm. The commercial material was fractionated by sieving through sequential sieves: <125 µ (Z8; fine), 125–250 µ (Z9; medium), and >250 µ (Z10; coarse). The DPVD of the fractions were calculated from the hysteresis loop according to the adsorption and desorption curves of Dollimore-Heal (DH) model. The adsorption cycle had produced dissimilar differential pore volume distribution patterns. For the 10-nm pore width, fine and medium fractions had a maximum pore volume up to 0.0016 cm³/g nm, while the coarse fraction had a little greater value up to 0.002 cm³/g nm. Moreover, the medium fraction had too many hills and valleys in the DPVD. However, the desorption cycle–based DH analysis did not show any marked variation in the DPVD pattern.  相似文献   

Alleviation of Salt Stress Adverse Effect and Enhancing Phenolic Anti-oxidant Content of Eggplant by Seaweed Extract     

Amira M. Hegazi  Amal M. El-Shraiy  A. A. Ghoname 《Gesunde Pflanzen》2015,67(1):21-31

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Streptococcus agalactiae isolates from cultured fishes in Malaysia manifesting low resistance pattern towards selected antibiotics          下载免费PDF全文

M A S Aisyhah  M N A Amal  M Zamri‐Saad  A Siti‐Zahrah  N N Shaqinah 《Journal of fish diseases》2015,38(12):1093-1098

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Serosurvey of five viruses in chickens on smallholdings in Bangladesh   总被引：1，自引：0，他引：1  

Biswas PK  Barua H  Uddin GM  Biswas D  Ahad A  Debnath NC 《Preventive veterinary medicine》2009,88(1):67-71

A serologic survey was undertaken in chickens in smallholdings in Bangladesh for avian influenza A virus (AIV), egg drop syndrome '76 virus (EDS'76V), infectious bronchitis virus (IBV), Newcastle disease virus (NDV) and reovirus (RV) in three phases: January 2002-May 2003, September 2003-August 2004, and August 2005-March 2006. Four hundred thirty-six sera collected in the 2nd phase, 295 in the first phase, 755 in the 1st plus 2nd phases and 295 in the 1st phase were investigated for AIV, EDS'76V, IBV and RV, respectively, using enzyme linked immunosorbent assays. All 854 sera collected in the three phases were screened for NDV using hemagglutination inhibition test. In chickens 20% were seropositive to AIV, 3% to EDS'76V, 74% to IBV, 88% to NDV, and 47% to RV. The seroprevalence in flocks was 23% to AIV, 6% to EDS'76V, 79% to IBV, 89% to NDV and 56% to RV. Twenty-five percent chickens had > or = 10log(2)HI titers to NDV.  相似文献   

Causes of loss of Sonali chickens on smallholder households in Bangladesh     

Biswas PK  Uddin GM  Barua H  Roy K  Biswas D  Ahad A  Debnath NC 《Preventive veterinary medicine》2006,76(3-4):185-195

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