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Mst. Nahid Akter Roshada Hashim Amalia Sutriana Mohd Nor Siti Azizah Md. Asaduzzaman 《Aquaculture Research》2019,50(3):786-797
The influence of dietary supplementation of Lactobacillus acidophilus, on growth performance, digestive enzyme activities, gut histomorphology and gut microflora were evaluated in juveniles striped catfish (Pangasianodon hypophthalmus). Five experimental diets were formulated by supplementing 0, 103, 105, 107 and 109 CFU/g L. acidophilus in fishmeal and casein‐based semi‐purified diet. Triplicate groups of striped catfish (21.69 ± 0.18 g) were stocked in 15 fiberglass tanks with stocking density of 25 individuals per tank and fed twice daily at 2.5% of the fish body weight for 12 weeks. Weight gain, specific growth rate, feed conversion ratio and the protein efficiency ratio were significantly higher in fish fed with 105 and 107CFU/g L. acidophilussupplemented diets compared with the other treatment groups. Compared with the control and fish fed low (103 CFU/g) L. acidophilus supplementation, those fed with 105 and 107 CFU/g had significantly higher (p < 0.05) apparent protein digestibility. Inclusion of L. acidophilus at 107 CFU/g diet significantly increased amylase, protease and lipase activities. Microscopic analysis showed that the villi length in both the anterior and posterior gut and microvilli length in the posterior gut increased significantly in fish fed L. acidophilus supplementation at 105 and 107 CFU/g of diet. The fish fed L. acidophilus supplemented diets significantly increased the total lactic acid bacteria counts in the gut of striped catfish compared with the control‐fed group. Based on gut histomorphology and growth performance, inclusion of L. acidophilus at 105 CFU/g appears to have the most positive effect on striped catfish farming. 相似文献
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Amalia Barone Domenico Carputo Giuliana La Rotonda Luigi Frusciante 《American Journal of Potato Research》1997,74(6):399-403
The cytological basis of 2n pollen production was studied in a diploidSolanum progeny coming from a cross between aS. tuberosum S. chacoense hybrid which does not produce2n pollen (T710) and a S.phurejar S. tuberosum hybrid (W5295.7) which does. Both parental clones showed anomalous orientation of spindles at Metaphase II of microsporogenesis, but only clone W5295.7 produced dyads. All plants of the progeny T710 × W5295.7 showed anomalous spindles at Metaphase II, but only 60% of them produced 2n pollen, thus evidencing a discrepancy between spindle anomalies and dyad/2n pollen production. This may suggest that both parallel and fused spindles are a necessary but insufficient condition for 2n pollen production, and that some other mechanisms expected to be more highly correlated with them may be involved in 2n pollen production in this population. 相似文献
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ABSTRACTThe aim of this work was to determine the changes in quality of cooked southern king crab (Lithodes santolla) meat during storage at 0°C for 5 and 10 days and ?20°C for 10, 30, 60, and 90 days. Chemical indices varied from the initial to the final storage time in refrigerated and frozen samples as follows: TVB-N, 15.8–29.2 and 15.8–23.2 mg/100 g; TMA, 0.0–8.3 and 0.0–1.2 mg/100 g; formaldehyde, 0.5–8.6 and 0.5–3.7 mg/kg; indole, 3.3–20.7 and 3.3–6.6 µg/100 g; lactic acid, 16.4–39.1 and 16.4–16.6 mg/100 g; and P ratio, 0–28.4 and 0–5.2%, respectively. The total nucleotide content rendered 6.77 µmol/g, with adenosine monophosphate (AMP) the main component. The nucleotide indices varied in refrigerated and frozen samples as follows: K, 7.37–27.5 and 7.37–10.3%; Ki, 58.3–86.5 and 58.3–82.7%; H, 30.2–54.9 and 30.2–38.0%; G, 19.4–57.8 and 19.4–15.8%; P, 17.2–42.3 and 17.2–14.7%; and Fr, 41.6–13.4 and 41.6–17.3%, respectively. Biogenic amines found at both storage temperatures were spermine, spermidine, tyramine, and agmatine. Spermine may be considered as a potential freshness index for refrigerated samples and spermidine for frozen storage. The tyramine level should be monitored. During the storage period tested, none of the chemical indices studied exceeded the legal limits. 相似文献
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Inês Guerreiro Helena Peres Carolina Castro Amalia Pérez‐Jiménez Manuela Castro‐Cunha Aires Oliva‐Teles 《Aquaculture Research》2014,45(2):289-298
The effect of dietary protein reduction balanced by an increase in carbohydrate (starch) level on growth performance, feed utilization and intermediary metabolism of Senegalese sole juveniles was evaluated at two temperatures, 12 and 18°C. For that purpose two isolipidic (16% lipids) diets were formulated to contain 550gkg?1 protein and 90gkg?1 starch (diet HP:LC), and 450gkg?1 protein and 200gkg?1 starch (diet LP:HC). Each experimental diet was fed to triplicate groups of 20 fish (initial body weight: 15.9g) within each temperature. Diets had no effect on growth and feed utilization. Temperature affected growth but not feed efficiency, with fish growing more at 18°C. Fatty acid synthetase and glutamate dehydrogenase activities were higher at 12°C than at 18°C while activity of the other measured enzymes was not affected by water temperature. Glucokinase and malic enzyme activities were lower while fructose 1,6‐bisphosphatase activity was higher in fish fed the HP:LC diet. Our data suggest that protein can be reduced from 550 gkg?1 to 450 gkg?1 by increasing starch level in high lipid diets for Senegalese sole juveniles without affecting overall performance. A reduction in protein content through an increase in dietary starch decreases hepatic gluconeogenesis. Increasing temperature from 12°C to 18°C improves fish growth but does not affect feed efficiency. 相似文献
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Carolina?Castro Ana?CoutoEmail author Amalia?Pérez-Jiménez Cláudia?R.?Serra Patricia?Díaz-Rosales Rui?Fernandes Geneviève?Corraze Stéphane?Panserat Aires?Oliva-Teles 《Fish physiology and biochemistry》2016,42(1):203-217
The impact of replacing circa 70 % fish oil (FO) by a vegetable oil (VO) blend (rapeseed, linseed, palm oils; 20:50:30) in diets for European sea bass juveniles (IBW 96 ± 0.8 g) was evaluated in terms of activities of digestive enzymes (amylase, lipase, alkaline phosphatase, trypsin and total alkaline proteases) in the anterior (AI) and posterior (PI) intestine and tissue morphology (pyloric caeca—PC, AI, PI, distal intestine—DI and liver). For that purpose, fish were fed the experimental diets for 36 days and then liver and intestine were sampled at 2, 6 and 24 h after the last meal. Alkaline protease characterization was also done in AI and PI at 6 h post-feeding. Dietary VO promoted higher alkaline phosphatase activity at 2 h post-feeding in the AI and at all sampling points in the PI. Total alkaline protease activity was higher at 6 h post-feeding in the PI of fish fed the FO diet. Identical number of bands was observed in zymograms of alkaline proteases of fish fed both diets. No alterations in the histomorphology of PC, AI, PI or DI were noticed in fish fed the VO diets, while in the liver a tendency towards increased hepatocyte vacuolization due to lipid accumulation was observed. Overall, and with the exception of a higher intestine alkaline phosphatase activity, 70 % FO replacement by a VO blend in diets for European sea bass resulted in no distinctive alterations on the postprandial pattern of digestive enzyme activities and intestine histomorphology. 相似文献
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