Development and characterization of genomic microsatellite markers in safflower (Carthamus tinctorius L.)     

Y. A. S. Hamdan  M. J. García‐Moreno  J. Redondo‐Nevado  L. Velasco  B. Pérez‐Vich 《Plant Breeding》2011,130(2):237-241

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Tench, Tinca tinca (L.), fish farms as a tool for environmental education     

Raquel P. Fernández Lo Faso  Ramón Velasco Gemio  José C. Escudero García  Encarnación García Ceballos-Zúñiga  Carmen Bueno  José Martín Gallardo 《Aquaculture International》2006,14(1-2):209-218

Since 1968, year of the May Revolution in France, UNESCO realised that a rapid separation was developing between the human race and nature, especially in big cities. This resulted in the development of numerous Environmental Education Programmes for children. Tench fish-farms are a particularly good tool for educating children in the structure and function of freshwater ecosystems. The ‘Vegas del Guadiana’ fish farm (Badajoz, Spain) Environmental Education Programme, is included in the Transborder Environmental Education Programme and in INTERREG II, an European Community Initiative whose objective is to promote cross-border, international and interregional co-operation to encourage balanced development in Europe. Preliminary results have been evaluated and presented in the present work. The analysis of the efficiency of the programme was measured in children from ages 6 to 12. The results showed no significant differences among sex (t-Student=0.07, p=0.9470) nor social surroundings (t-Student=0.87, p=0.3910) but there were significant differences when comparing different ages (ANOVA, p=0.0437). In all cases, a significant increase in the knowledge and concern about the freshwater environment was found in children (Paired t-Student=−10.55, p=0.0001).  相似文献   

Assessing the role of the aquatic Natura 2000 network to protect both freshwater European species of community interest and threatened species in a Mediterranean region     

David Sánchez-Fernández  Rocío A. Baquero  Luis Velasco  Antonio Aranda  Graciela G. Nicola 《水产资源保护:海洋与淡水生态系统》2021,31(7):1901-1911

1. The European Natura 2000 (N2K) network of protected areas stands out as the main conservation strategy in the European Union to preserve biodiversity under the auspices of the Convention on Biological Diversity.
2. The management of N2K sites is mainly focused on protecting the biological elements as Special Areas of Conservation for habitats or species listed in the European Habitats Directive, or as Special Protection Areas for birds listed in the European Birds Directive for which the site was designated. It seems urgent, therefore, to discriminate which N2K sites are really providing effective protection for aquatic biodiversity.
3. Although the main objective of N2K is to protect species listed in the Birds and Habitats Directives, the conservation status of threatened species included in the European Red List of the IUCN must also be a concern for the European Union.
4. Focusing on Central Spain as a case study, the aim of this work was to evaluate how far aquatic N2K (i.e. N2K sites designated for the occurrence of freshwater elements) is effective in protecting both those aquatic species in the Directives [referred to here as Species of Community Importance (SCI) and threatened species (TS)].
5. Most of the N2K sites in the study area have been designated using aquatic elements, which means that N2K could theoretically provide appropriate mechanisms to preserve aquatic biodiversity. However, N2K provides a low percentage of overlap with some of the hotspots identified for both TS and SCI. Surprisingly, TS are in general similarly or even better represented than SCI by N2K.
6. N2K may provide good coverage for aquatic biodiversity, mainly by acting as an umbrella for other species, but it could be improved by addressing some spatial gaps, thereby making it more relevant to current challenges such as climate change and other human impacts.

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Candidatus Chloracidobacterium thermophilum: an aerobic phototrophic Acidobacterium     

Bryant DA  Costas AM  Maresca JA  Chew AG  Klatt CG  Bateson MM  Tallon LJ  Hostetler J  Nelson WC  Heidelberg JF  Ward DM 《Science (New York, N.Y.)》2007,317(5837):523-526

Only five bacterial phyla with members capable of chlorophyll (Chl)-based phototrophy are presently known. Metagenomic data from the phototrophic microbial mats of alkaline siliceous hot springs in Yellowstone National Park revealed the existence of a distinctive bacteriochlorophyll (BChl)-synthesizing, phototrophic bacterium. A highly enriched culture of this bacterium grew photoheterotrophically, synthesized BChls a and c under oxic conditions, and had chlorosomes and type 1 reaction centers. "Candidatus Chloracidobacterium thermophilum" is a BChl-producing member of the poorly characterized phylum Acidobacteria.  相似文献   

Detection and characterization of Streptomyces causing potato common scab in Western Europe     

R. Flores-González  I. Velasco  F. Montes 《Plant pathology》2008,57(1):162-169

A PCR-based diagnostic method was developed for direct detection from tuber lesions of pathogenic Streptomyces causing common scab of potato. Primers were designed to amplify a fragment of the txtAB ( txtA and txtB ) genes, which are pathogenicity determinants in the main pathogenic Streptomyces species. The method was evaluated on 84 naturally infected potato samples, comprising 19 potato cultivars, harvested in the years from 2000 to 2004 in the Netherlands, the UK, France, Germany and Spain. Pathogenic Streptomyces in tuber lesions were detected by PCR in 70 samples and were also successfully isolated from these 70 samples. All pathogenic isolates showed the basic general phenotypic traits of the S. scabiei phenetic cluster. RFLP analysis of amplified rRNA sequences, together with carbon source utilization and repetitive BOX profiles, allowed most isolates to be assigned to S. europaeiscabiei , which emerged as the main cause of potato common scab in Western Europe.  相似文献   

Pharmacokinetics of an injectable long-acting formulation of doxycycline hyclate in dogs     

Lilia Gutiérrez  Zazil-Ha Velasco  Carlos Vázquez  Dinorah Vargas  Héctor Sumano 《Acta veterinaria Scandinavica》2012,54(1):35

Based on its PK/PD ratios, doxycycline hyclate (DOX-h), a time-dependant antibacterial, is ideally expected to achieve both sustained plasma drug concentrations at or slightly above the MIC level for as long as possible between dosing intervals. Pursuing this end, a poloxamer-based matrix was used to produce a long-acting injectable preparation (DOX-h-LA) and its serum concentrations vs. time profile investigated after its SC injection to dogs (≤ 0.3 mL per injection site), and results compared with the oral (PO) and IV pharmacokinetics of DOX-h, prepared as tablet or as freshly made solution. A crossover (4 x 4 x 4) study design was employed with 12 Mongrel dogs, with washout periods of 21 days, and at dose of 10 mg/kg in all cases. DOX-h-LA showed the greatest values for bioavailability (199.48%); maximum serum concentration (Cmax) value was 2.8 ± 0.3 with a time to reach Cmax (Tmax) of 2.11 ± 0.12 h and an elimination half-life of 133.61 ± 6.32 h. Considering minimum effective serum concentration of 0.5 μg/mL, a dose-interval of at least 1 week h can be achieved for DOX-h-LA, and only 48 h and 24 h after the IV or PO administration of DOX-h as a solution or as tablets, respectively. A non-painful small bulge, apparently non-inflammatory could be distinguished at injection sites. These lumps dissipated completely in 30 days in all cases.  相似文献   

Biological characterization of Tomato leaf curl New Delhi virus from Spain          下载免费PDF全文

L. Velasco  D. Janssen 《Plant pathology》2017,66(3):376-382

Tomato leaf curl New Delhi virus (ToLCNDV; family Geminiviridae, genus Begomovirus) is an emerging virus in horticulture crops in Asia, and has recently been introduced in Spain, Tunisia and Italy. No betasatellite DNA was detected in infected tomato and zucchini squash samples from Spain, and agroinoculated viral DNA‐A and DNA‐B were sufficient to reproduce symptoms in plants of both crop species. Infected tomato and zucchini squash plants also served as inoculum sources for efficient transmission either mechanically or using Bemisia tabaci whiteflies. Cucumber, melon, watermelon, zucchini squash, tomato, eggplant and pepper, but not common bean, were readily infected using viruliferous whiteflies and expressed symptoms 8–15 days post‐inoculation. New full‐length sequences from zucchini squash and tomato indicated a high genetic homogeneity (>99% sequence identity) in the ToLCNDV populations in Spain, pointing to a single recent introduction event.  相似文献   

Fitness costs and stability of Cry1Fa resistance in Brazilian populations of Spodoptera frugiperda          下载免费PDF全文

Oscar F Santos‐Amaya  Clébson S Tavares  João Victor C Rodrigues  Silverio O Campos  Raul Narciso C Guedes  Analiza P Alves  Eliseu José G Pereira 《Pest management science》2017,73(1):35-43

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Rapid determination of crocetin esters and picrocrocin from saffron spice (Crocus sativus L.) using UV-visible spectrophotometry for quality control     

Sánchez AM  Carmona M  Zalacain A  Carot JM  Jabaloyes JM  Alonso GL 《Journal of agricultural and food chemistry》2008,56(9):3167-3175

The aim of this work was the development of multivariate models able to determine the content of the main crocetin esters and picrocrocin from spectrophotometric data that could be used for routine quality control of saffron. These compounds were determined with HPLC in Spanish saffron, and their absorbance spectra from 190 to 700 nm were simultaneously monitored. Partial least-squares regression (PLSR) models have been obtained and applied to the determination of individual crocetin esters, to the sum of crocetin esters, and to picrocrocin. A modification of the Kennard-Stone algorithm was used to divide the pool of samples into calibration and validation subsets. The best predictions were obtained with the sum of crocetin esters model, followed by the model for cis-crocetin (beta- D-glucosyl)-(beta- D-gentiobiosyl) ester, trans-crocetin di-(beta- D-gentiobiosyl) ester, and trans-crocetin (beta- D-glucosyl)-(beta- D-gentiobiosyl) ester, whereas the worst predictions were found with the picrocrocin and trans-crocetin (beta- D-gentiobiosyl) ester models. These models may enhance quality control in saffron enterprises.  相似文献   

Inheritance of high oleic acid content in safflower     

Yamen A. S. Hamdan  Begoña Pérez-Vich  Leonardo Velasco  José M. Fernández-Martínez 《Euphytica》2009,168(1):61-69

Safflower (Carthamus tinctorius L.) oil with high oleic acid content (>75%) has a great value for both food and non-food uses. The trait has been reported to be environmentally stable and controlled by recessive alleles at one single gene Ol, even though the influence of modifying genes has been suggested. Additionally, germplasm with higher oleic acid content (>85%) has been reported. The objective of the present research was to study the inheritance of high oleic acid content in genetic sources with both levels of high oleic acid content (>75 and >85%, respectively). A genetic study was conducted by crossing the nuclear male-sterile line CL1 (18% oleic acid) and the high oleic acid lines CR-6 (80%) and CR-9 (87%). The evaluation of the F₁ and F₂ seed generations of the crosses CL1 × CR-6 and CL1 × CR-9 indicated that in both cases the high oleic acid trait was controlled by partially recessive alleles at a single locus. The observation of F₂, F₃, and F₄ segregants with high oleic acid phenotype but lower oleic acid levels than the parents revealed the presence of modifying genes affecting the trait. Crosses between the two high oleic acid lines produced no transgressive segregation other than that caused by the mentioned modifying genes, suggesting that the high oleic acid lines CR-6 and CR-9 share the same alleles at the Ol locus. Differences for oleic acid content between both lines were hypothesized to be produced by the accumulation of genes with a minor effect on the trait.  相似文献