Total chemically available (free and intrachain) lysine and furosine in pea, bean, and lentil sprouts     

Rodríguez C  Frías J  Vidal-Valverde C  Hernández A 《Journal of agricultural and food chemistry》2007,55(25):10275-10280

The effect of the germination of peas, beans, and lentils under differing conditions of illumination for different times on parameters linked to the Maillard reaction (chemically available free and intrachain lysine, lysine availability, and furosine) was evaluated. The chemically available free lysine content in the raw seeds of the three legumes was quite small compared to the chemically available intrachain lysine content, and furosine was detectable only in the beans and the lentils. The effect of germination was to increase lysine availability compared with levels in the raw seeds in all of the germinated samples, the smallest increase taking place in the lentils. In addition, furosine became detectable in all of the germinated samples. Quantities varied depending on the germination conditions but in all cases were higher than the quantities observed in the raw seeds. Linear correlations were observed to exist between some of the parameters considered in the three legumes tested.  相似文献   

Effects of four egg desticking procedures on hatching rate and further survival and growth of larvae in the tench (Tinca tinca L.)     

Jose M Carral  Jesús D Celada  Maria Sáez-Royuela  Ruth Rodríguez  Amelia Aguilera  & Pedro Melendre 《Aquaculture Research》2006,37(6):632-636

Four desticking procedures for tench eggs (A: tannic acid solution (1 g L⁻¹) for 15 s; B: alcalase enzyme solution (8 mL L⁻¹) for 60 s; C: alcalase enzyme solution (15 mL L⁻¹) for 120 s; D: Woynarovich and Woynarovich (1980) solution for 58 min followed by tannic acid solution (1 g L⁻¹) for 15 s) were tested to obtain data about influence on embryo survival to hatching stage and further survival and growth of the larvae. In the tannic acid and Woynarovich and Woynarovich (1980) treatment (A and D) few eggs stuck together and some were adhered to the incubator walls, whereas in the alcalase treatments (B and C) eggs neither stuck together nor adhered to the incubator walls. Percentages of hatched larvae did not show significant differences (mean values ranged between 47.4% in treatment A to 37.0% in treatment C). Larvae deformities observed were <0.5% in all cases. There were no significant differences among survival and growth rates of the larvae from different egg desticking origin, reaching, after 30 days, mean survival values around 90% and total length and weight of 12.5 mm and 19 mg respectively.  相似文献   

Myocardial injury in dogs with snake envenomation and its relation to systemic inflammation     

Rebecca Langhorn  Frida Persson  Bjrn blad  Amelia Goddard  Johan P. Schoeman  Jakob L. Willesen  Inge Tarnow  Mads Kjelgaard‐Hansen 《Journal of Veterinary Emergency and Critical Care》2014,24(2):174-181

  相似文献   

Digestive enzyme activity during early larval development of the Cuban gar Atractosteus tristoechus   总被引：1，自引：0，他引：1  

Y. Comabella  R. Mendoza  C. Aguilera  O. Carrillo  A. Hurtado  T. García-Galano 《Fish physiology and biochemistry》2006,32(2):147-157

The ontogenesis of digestive enzymes (proteases, amylases, lipases, and phosphatases) in Cuban gar Atractosteus tristoechus was determined in larvae between 5 and 18 days after hatching (DAH). Variations in specific activities of most enzymes were related to the transition from endogenous to exogenous feeding and to the transition from the larval to the juvenile stage. Alkaline protease activity was not detected until 8 DAH in contrast to acid protease activity, which was quantifiable at 5 DAH. Acid protease activity was consistently higher than alkaline protease activity, indicating the presence of a functional stomach in the early stages of larval development. The acid protease activities of larvae and adults were compared by means of zymogram analysis. Four acid protease bands were found in adults (two more than in larvae). This result is the first time that more than one band of acid proteolytic activity has been found in Lepisosteidae. High lipase activity indicated the importance of lipid utilization, particularly during yolk-sac absorption. In contrast to the other enzymes studied, amylase activity was consistently low, probably due to the strictly carnivorous diet of gar larvae and their low capacity for carbohydrate digestion. High activities of aminopeptidase and acid and alkaline phosphatases suggest intestinal absorption. This result, together with the existence of a short gut and a lower proteolytic activity in the intestine than in the stomach, suggest that most of the proteolytic activity takes place in the stomach, while the primary function of the intestine is nutrient uptake.  相似文献   

The anatomy of the world's largest extinct rodent     

Sánchez-Villagra MR  Aguilera O  Horovitz I 《Science (New York, N.Y.)》2003,301(5640):1708-1710

Phoberomys is reported to be the largest rodent that ever existed, although it has been known only from isolated teeth and fragmentary postcranial bones. An exceptionally complete skeleton of Phoberomys pattersoni was discovered in a rich locality of fossil vertebrates in the Upper Miocene of Venezuela. Reliable body mass estimates yield approximately 700 kilograms, more than 10 times the mass of the largest living rodent, the capybara. With Phoberomys, Rodentia becomes one of the mammalian orders with the largest size range, second only to diprotodontian marsupials. Several postcranial features support an evolutionary relationship of Phoberomys with pakaranas from the South American rodent radiation. The associated fossil fauna is diverse and suggests that Phoberomys lived in marginal lagoons and wetlands.  相似文献   

Fluorescence detection of aluminum in arbuscular mycorrhizal fungal structures and glomalin using confocal laser scanning microscopy     

Paula Aguilera  Fernando Borie  Alex Seguel  Pablo Cornejo 《Soil biology & biochemistry》2011,43(12):2427-2431

Arbuscular mycorrhizal spores and glomalin-related soil protein (GRSP) isolated from acid soils were analyzed using confocal laser scanning microscopy (CLSM) for Al detection. Mycorrhizal structures of Glomus intraradices produced under in vitro conditions as well as spores and GRSP from neutral and Cu-polluted soils were used as contrasting criteria. Spores and GRSP from soils with 7 and 70% Al saturation showed autofluorescence which increased especially at the highest soil Al level and when Al³⁺ solution was added. G.intraradices spores showed fluorescence only when exogenous Al³⁺ was added. On the contrary, spores and GRSP from neutral and Cu-polluted soils showed little or no significant fluorescence. This fluorescence shown by fungal structures and GRSP when subjected to high Al (of endogenous or exogenous origin) suggest a high capacity for Al immobilization, which could be an effective way to reduce Al activity and phytotoxicity in acid soils.  相似文献   

Contradictions in host plant resistance to pests: spider mite (Tetranychus urticae Koch) behaviour undermines the potential resistance of smooth-leaved cotton (Gossypium hirsutum L.)     

Reddall AA  Sadras VO  Wilson LJ  Gregg PC 《Pest management science》2011,67(3):360-369

BACKGROUND: Two‐spotted spider mites (Tetranychus urticae Koch) oviposit near leaf veins or in leaf folds on the undersides of cotton (Gossypium hirsutum L.) leaves where the humid boundary layer offers protection from desiccation. The authors predicted that the boundary layer of glabrous cotton leaves should be shallower than that of hairy leaves, providing some resistance to mites. The dynamics of mite populations, leaf damage, leaf gas exchange and crop yield on two leaf hair isolines (smooth versus hairy) in two genetic backgrounds was assessed. RESULTS: Mite colonies developed faster on the hairy leaf isolines, but leaf damage per mite was higher in smooth leaf isolines, indicating more intense damage. A 50% reduction in photosynthesis on the hairy isolines required 1.8 times more mites than smooth leaves. The yield of cotton was reduced in + mite treatments, but the magnitude of reduction was similar for hairy and smooth isolines. CONCLUSION: Paradoxically, the relative inhospitality of glabrous leaves may have induced mites to concentrate in protected leaf sections, causing more localised and more severe damage, negating the yield benefits from fewer mites. These results highlight interactions between leaf microenvironment, pest behaviour and plant productivity that may have implications for other instances of plant resistance. Copyright © 2010 Society of Chemical Industry  相似文献   

Supercritical fluid extraction of pesticides from a table-ready food composite of plant origin (gazpacho)   总被引：1，自引：0，他引：1  

Aguilera A  Brotons M  Rodríguez M  Valverde A 《Journal of agricultural and food chemistry》2003,51(19):5616-5621

Supercritical fluid carbon dioxide extraction (SFE) has been evaluated for the extraction of 17 organohalogen and organophosphate pesticides in gazpacho (a table-ready food composite containing crude vegetables, white bread, vegetable oil, water, and other minor components) using anhydrous magnesium sulfate as drying agent. The effects of different parameters, such as fat content in gazpacho composites, magnesium sulfate/gazpacho ratio, supercritical fluid volume, pressure, temperature, and static modifier additions, on SFE recoveries from spiked gazpacho samples have been studied. Analyses were performed by gas chromatography (GC) with flame photometric (FPD), electron capture (ECD), and mass spectrometry (MSD) detectors. In most experiments, recoveries obtained for the nonpolar organohalogen pesticides were lower than those obtained for the most polar organophosphate pesticides, but overall pesticide recoveries determined by using the optimal SFE conditions indicate that SFE could be used to determine pesticide residue levels in gazpacho.  相似文献   

Clinical and Clinicopathologic Characteristics of Ovine GM-1 Gangliosidosis     

Robert D. Murnane DVM  PhD    David J. Prieur  Amelia J. Ahern-Rindell  Larry D. Holler  Steven M. Parish 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(3):221-223

Ovine GM-1 gangliosidosis is an inherited lysosomal storage disease. Nine lambs affected with the disease were studied to characterize clinical signs and to determine if there were any pathognomonic clinicopathologic abnormalities. Evaluation included physical, ophthalmic, and neurologic examinations, complete blood counts, serum enzyme and electrolyte analyses, urinalyses, cerebrospinal fluid analyses, blood gas analyses, roentgenograms, electromyograms, and electrocardiograms. Two affected lambs had clinicopathologic tests performed before and after the onset of clinical signs. The only consistent abnormalities recognized were nonspecific signs referable to the central nervous system; predominantly ataxia, conscious proprioceptive deficit most severe in the hind limbs, blindness, and recumbency. Lambs continued to eat and drink, though at diminished levels and with loss of body condition. It was concluded that there are no pathognomonic clinicopathologic abnormalities associated with ovine GM-1 gangliosidosis, and antemortem diagnosis requires enzyme assay of leukocytes or cultured fibroblasts, or lectin histochemistry of tissues obtained by biopsy. Lysosomal storage diseases should be considered among the differential diagnoses in young animals presenting with early neonatal death or with nonspecific neurological signs, in concert with an absence of diagnostic clinicopathologic findings.  相似文献   

Fractures of the humerus   总被引：2，自引：0，他引：2  

Simpson AM 《Clinical Techniques in Small Animal Practice》2004,19(3):120-127

Humeral fractures are the least common long bone fracture in small animals. The low incidence of these fractures combined with the unique anatomy of the humerus makes repair of humeral fractures challenging. This chapter provides a review of humeral fracture management.  相似文献