罗马尼亚沙棘资源建设和利用状况     

郭红艳  胡建忠  Proorocu Angel 《国际沙棘研究与开发》2007,5(3):34-39

沙棘,亚欧种,1753年植物学家Karl von Linné发现了沙棘并分类为"植物志"的第1023种.沙棘(Hippophae rhamnoides)被认为是希腊语"闪闪发亮的马"的语源.罗马尼亚的专家论证了沙棘对动物健康的影响,特别是皮肤方面,沙棘还有着消除肠内病菌的作用.  相似文献   

Highly sensitive enzyme-linked immunosorbent assay for chlorpyrifos. Application to olive oil analysis     

Brun EM  Garcés-García M  Puchades R  Maquieira A 《Journal of agricultural and food chemistry》2005,53(24):9352-9360

Highly sensitive enzyme-linked immunoassays for chlorpyrifos, one of the most applied insecticides, are presented. Several haptens were synthesized for immunoreagent production and ELISA development. The best immunoassays obtained are based on an indirect coated-plate immunoassay format. Two assays were optimized; one shows a limit of detection of 0.3 ng L(-1), an I50 of 271 ng L(-1), and a dynamic range between 4 and 16 474 ng L(-1). The other one has a limit of detection of 0.07 ng L(-1), an I50 of 7 ng L(-1), and a dynamic range between 0.4 and 302 ng L(-1). The assays were used to quantify chlorpyrifos in olive oil using a simple and rapid sample extraction procedure. The good recoveries achieved in both assays (109% mean value) and the agreement with values given by the GC reference method (110% mean value) indicate their potential for either screening or laboratory quantification.  相似文献   

Effect of the rootstock and interstock grafted in lemon tree (Citrus limon (L.) Burm.) on the flavonoid content of lemon juice     

Gil-Izquierdo A  Riquelme MT  Porras I  Ferreres F 《Journal of agricultural and food chemistry》2004,52(2):324-331

The grafting of the rootstock with the lemon tree is an agronomical technique used to improve production and/or quality of the fruit. The interstock has been used with different fruit trees to modulate the tree size, fruit production and quality, and the aging of the tree. The lemon trees grafted with interstocks increase their longevity, lemon production and quality; interstocks are also used to decrease the thickness of the trunk at the grafting point. This enlarging of the trunk provokes a decrease of the sap flow. In our study, "Verna" lemon trees were grafted with interstock between the rootstock and the lemon tree to follow the flavonoid content of the lemon juice. The lemon juice was obtained from the lemons collected of the grafted lemon trees. Two types of rootstocks were used: Citrus aurantium L. and Citrus macrophylla L. Seven interstocks from five cultivars of orange tree, one cultivar of lime tree, and one cultivar of tangerine tree were used. "Verna" lemon trees were also grafted directly to the rootstock. The rootstock was more important agronomic factor than the interstock on the total flavonoid content of lemon juice. The interstock grafting had only a small influence on the flavonoid content of the lemon juice, and it modulated the individual flavonoid content. Citrus aurantium L. rootstock and "Berna" and "Washington Navel" interstocks were the most appropriate to graft in the lemon tree. This interstock grafting technique does not increase the flavonoid content of the lemon juice. Regarding the individual flavonoids, the 6,8-di-C-glucosyl diosmetin was the most affected flavonoid by the type of rootstock used. The interstock used is able to alter the individual quantitative flavonoid order of eriocitrin, diosmin, and hesperidin. In addition, the HPLC-ESI/MS(n) analyses provided the identification of two new flavonoids in the lemon juice: Quercetin 3-O-rutinoside-7-O-glucoside and chrysoeriol 6,8-di-C-glucoside (stellarin-2). The occurrence of apigenin 6,8-di-C-glucoside (vicenin-2), eriodictyol 7-O-rutinoside, 6,8-di-C-glucosyl diosmetin, hesperetin 7-O-rutinoside, homoeriodictyol 7-O-rutinoside and diosmetin 7-O-rutinoside was also confirmed in lemon juice by this technique.  相似文献   

Use of a byproduct of magnesium oxide production to precipitate phosphorus and nitrogen as struvite from wastewater treatment liquors   总被引：3，自引：0，他引：3  

Quintana M  Colmenarejo MF  Barrera J  García G  García E  Bustos A 《Journal of agricultural and food chemistry》2004,52(2):294-299

This paper describes a series of experiments designed to recover phosphorus and nitrogen from sewage in the form of struvite (MgNH(4)PO(4).6H(2)O), a potential fertilizer. Nitrogen and phosphate were recovered from a filtrate of digested sludge dewatered at the Arroyo del Soto Waste Water Treatment Plant (WWTP) (Madrid, Spain). A byproduct of the Spanish magnesite mining and MgO production industry was used as the magnesium source. The precipitating performance of this byproduct was compared to that of conventional chemical reagents such as pure MgO. The precipitates obtained were subjected to chemical, light microscopy, and X-ray diffraction analysis. The findings indicate the precipitate recovered using this byproduct contains several minerals with a predominance of struvite. Optimal purity ( approximately 80% struvite) was achieved using the sieved <0.04 mm grain size fraction of the byproduct at doses corresponding to a molar Mg:P ratio of 1.6.  相似文献   

In vitro gastrointestinal digestion study of pomegranate juice phenolic compounds, anthocyanins, and vitamin C   总被引：5，自引：0，他引：5  

Pérez-Vicente A  Gil-Izquierdo A  García-Viguera C 《Journal of agricultural and food chemistry》2002,50(8):2308-2312

Pomegranate is an important source of bioactive compounds, such as anthocyanins, other phenolic compounds, and ascorbic acid. In the present work an in vitro availability method has been used to assay the influence of the physiological conditions in the stomach and small intestine. This method enables the study of the release of anthocyanins, vitamin C, and total phenols from the pomegranate juice and their transformations during gastrointestinal digestion. Results have shown that pomegranate phenolic compounds are available during the digestion in a quite high amount (29%). Nevertheless, due to pH, anthocyanins are largely transformed into non-red forms and/or degraded (97%), and similar results are obtained for vitamin C (>95% degradation).  相似文献   

Novel inhibitors of the mitochondrial respiratory chain: oximes and pyrrolines isolated from Penicillium brevicompactum and synthetic analogues     

Cantín A  López-Gresa MP  González MC  Moya P  Miranda MA  Primo J  Romero V  Peris E  Estornell E 《Journal of agricultural and food chemistry》2005,53(21):8296-8301

The capacity of inhibition of the mammalian mitochondrial respiratory chain of brevioxime 5a, a natural insecticide compound isolated from Penicillium brevicompactum culture broth, and another 15 analogue compounds, other oximes 5b and 5c; two diastereomeric pyrrolidines 1c' and 1c' '; five pyrrolines 3c', 3c' ' (diastereomers between them), 3a, 3b, and 6; two oxazines 4c' and 4c' ' (also diastereomers between them); and four pyrrol derivatives 7-10, are analyzed in this paper. Compounds 3b, 3c', 3c' ', 4c', 4c' ', 5b, 5c, 6, and 10 were found to be inhibitors of the integrated electron transfer chain (NADH oxidase activity) in beef heart submitochondrial particles (SMP), establishing that all of them except compound 3b and 6 only affected to complex I of the mitochondrial respiratory chain. The most potent product was 5b, with an IC50 of 0.27 microM, similar to the IC50 values of other known complex I inhibitors. The diastereomeric pairs 1c'/1c' ', 3c'/3c' ', 4c'/4c' ', and 5c have not been previously described. Chemical characterization, on the basis of spectral data, is also shown.  相似文献   

Population genetic structure of <Emphasis Type="Italic">Juniperus phoenicea</Emphasis> (Cupressaceae) in the western Mediterranean Basin: gradient of diversity on a broad geographical scale     

Artur Dzialuk  Małgorzata Mazur  Krystyna Boratyńska  Jose M. Montserrat  Angel Romo  Adam Boratyński 《Annals of Forest Science》2011,68(8):1341-1350

• Introduction, Material and Methods  

The genetic structure and diversity of ten natural populations of Juniperus phoenicea L. from the western part of the species range have been studied using random amplified polymorphic DNA (RAPD) markers.  相似文献   

The use of hand-raised psittacines for reintroduction: a case study of scarlet macaws (Ara macao) in Peru and Costa Rica     

Donald Brightsmith  Jenifer Hilburn  Alvaro del Campo  Janice Boyd  Richard Frisius  Federico Guillen 《Biological conservation》2005,121(3):465-472

This study reports on three scarlet macaw (Ara macao) reintroduction projects using hand-raised birds in Peru and Costa Rica. The habitats at the release sites ranged from pristine tropical forest to forest fragments in an agricultural matrix. The combined first-year survival was 74% and the annual post first-year survival was 96%. Survival rates were very high despite a wide range in predator communities. Number of birds released explained 70% of the variation in survival with birds from larger releases having higher survival rates. Behavioral evidence suggests that birds established at the site facilitated survival of later releases. Breeding attempts were recorded at all three sites and hand-raised birds with wild mates successfully fledged young in Peru. Supplemental feeding post-release played an important role in keeping the birds near the release site and facilitating social interactions. This work shows that properly socialized hand-raised macaws can survive and breed in the wild but that ex-pets are not good release candidates.  相似文献   

Resveratrol treatment controls microbial flora, prolongs shelf life, and preserves nutritional quality of fruit     

Jiménez JB  Orea JM  Montero C  Ureña AG  Navas E  Slowing K  Gómez-Serranillos MP  Carretero E  De Martinis D 《Journal of agricultural and food chemistry》2005,53(5):1526-1530

Resveratrol is known as a grapevine secondary metabolite with fungicide activity. Its exogenous application on harvested grapes resulted in the reduction of microbial flora growth, and consequently, prolonged shelf life, without affecting the nutritional quality of the fruit. Resveratrol treatment also resulted in being effective on fruit that normally does not accumulate such metabolites as, for example, tomatoes, apples, avocado pears, and peppers. As a result, all treated fruits maintained their post-harvest quality and health longer than the untreated ones. This study demonstrates the potential use of resveratrol as a natural pesticide to reduce post-harvest fungi development on a broad spectrum of fruit types.  相似文献   

Development of a novel and automated fluorescent immunoassay for the analysis of beta-lactam antibiotics   总被引：4，自引：0，他引：4  

Benito-Peña E  Moreno-Bondi MC  Orellana G  Maquieira A  van Amerongen A 《Journal of agricultural and food chemistry》2005,53(17):6635-6642

An automated immunosensor for the rapid and sensitive analysis of penicillin type beta-lactam antibiotics has been developed and optimized. An immunogen was prepared by coupling the common structure of the penicillanic beta-lactam antibiotics, i.e., 6-aminopenicillanic acid to keyhole limpet hemocyanin. Polyclonal antibodies raised in rabbits after immunization with this conjugate have been applied for the development of a competitive fluoroimmunoassay (FIA), using a novel fluorescent penicillin {[2S,5R,6R]-3,3-dimethyl-7-oxo-6-[(pyren-1ylacetyl)amino]-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxilic acid, PAAP} as the tracer and penicillin G as the reference antibiotic. Protein A/G covalently bound to an azlactone-activated polymeric support was used for the orientated capture of the antibody-antigen immunocomplexes. Upon desorption from the immunosupport, the emission signal generated by the PAAP-Ab complexes is related to the antibiotic concentration in the sample. The 50% binding inhibition concentration of penicillin G standard curves was at 30 ng mL(-)(1) with a detection limit (10% binding inhibition) of 2.4 ng mL(-)(1) and a dynamic range from 6.0 to 191 ng mL(-)(1) (20-80% binding inhibition) penicillin G. The generic nature of the antiserum was shown by good relative cross-reactivities with penicillin type beta-lactam antibiotics such as amoxicillin (50%), ampicillin (47%), and penicillin V (145%) and a lower response to the isoxazolyl penicillins such as oxacillin, cloxacillin, and dicloxacillin. No cross-reactivity was obtained for cephalosporin type beta-lactam antibiotics (cephapirin), cloramphenicol, or fluoroquinolones (enrofloxacin and ciprofloxacin). The total analysis time was 23 min per determination, and the immunoreactor could be reused for more than 200 cycles without significant loss of activity. The immunosensor has been successfully applied to the direct analysis of penicillin G and amoxicillin in spiked influent and effluent sewage treatment plant water samples with excellent recoveries (mean values for penicillin G and amoxicillin, 99 and 105%, respectively). Results displayed by comparative analysis of the immunosensor with a chromatographic procedure for penicillins showed excellent agreement between both methods.  相似文献