全文获取类型
收费全文 | 847篇 |
免费 | 28篇 |
国内免费 | 1篇 |
专业分类
林业 | 58篇 |
农学 | 129篇 |
基础科学 | 17篇 |
213篇 | |
综合类 | 54篇 |
农作物 | 49篇 |
水产渔业 | 57篇 |
畜牧兽医 | 156篇 |
园艺 | 21篇 |
植物保护 | 122篇 |
出版年
2023年 | 10篇 |
2022年 | 8篇 |
2021年 | 13篇 |
2020年 | 19篇 |
2019年 | 19篇 |
2018年 | 19篇 |
2017年 | 25篇 |
2016年 | 29篇 |
2015年 | 22篇 |
2014年 | 16篇 |
2013年 | 63篇 |
2012年 | 37篇 |
2011年 | 55篇 |
2010年 | 31篇 |
2009年 | 35篇 |
2008年 | 40篇 |
2007年 | 47篇 |
2006年 | 29篇 |
2005年 | 30篇 |
2004年 | 9篇 |
2003年 | 26篇 |
2002年 | 23篇 |
2001年 | 21篇 |
2000年 | 24篇 |
1999年 | 14篇 |
1998年 | 7篇 |
1997年 | 10篇 |
1996年 | 13篇 |
1995年 | 10篇 |
1994年 | 9篇 |
1993年 | 11篇 |
1992年 | 8篇 |
1991年 | 10篇 |
1990年 | 8篇 |
1989年 | 15篇 |
1988年 | 8篇 |
1987年 | 5篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 10篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 4篇 |
1979年 | 11篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1971年 | 4篇 |
1969年 | 6篇 |
1967年 | 4篇 |
排序方式: 共有876条查询结果,搜索用时 453 毫秒
71.
Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient 总被引:27,自引:0,他引:27
E Santos D Martin-Zanca E P Reddy M A Pierotti G Della Porta M Barbacid 《Science (New York, N.Y.)》1984,223(4637):661-664
A single genetic alteration, a guanine-to-cytosine transversion, is responsible for the acquisition of malignant properties by K-ras genes of two human tumor cell lines established from carcinomas of the bladder (A1698) and lung (A2182). As a consequence, arginine instead of the normal glycine is incorporated into the K-ras-coded p21 proteins at amino acid position 12. This mutation creates a restriction enzyme polymorphism that can be used to screen human cells for transforming K-ras genes. This approach was used to identify the mutational event responsible for the malignant activation of a K-ras oncogene in a squamous cell lung carcinoma of a 66-year-old man; this point mutation was not present in either the normal bronchial or parenchymal tissue or in the blood lymphocytes. Hence, malignant activation of a ras oncogene appears to be specifically associated with the development of a human neoplasm. 相似文献
72.
73.
74.
Fluid flows that are smooth at low speeds become unstable and then turbulent at higher speeds. This phenomenon has traditionally been investigated by linearizing the equations of flow and testing for unstable eigenvalues of the linearized problem, but the results of such investigations agree poorly in many cases with experiments. Nevertheless, linear effects play a central role in hydrodynamic instability. A reconciliation of these findings with the traditional analysis is presented based on the "pseudospectra" of the linearized problem, which imply that small perturbations to the smooth flow may be amplified by factors on the order of 10(5) by a linear mechanism even though all the eigenmodes decay monotonically. The methods suggested here apply also to other problems in the mathematical sciences that involve nonorthogonal eigenfunctions. 相似文献
75.
Penultimate and last instar larvae of the common citrus swallowtail butterfly, Papilio demoleus L. (Papilionidae: Lepidoptera), were treated with different doses of a novel juvenoid, diofenolan. Several deformities were
observed as a result of topical administration of diofenolan which include delay in larval–larval and larval–pupal ecdysis,
ecdysial failure, mortality, severe reduction in pupation, deformed pupae and complete inhibition of adult emergence. The
juvenoid diofenolan severely hampers the normal growth, development and metamorphosis of P. demoleus and can be incorporated into integrated pest management programs for successful control of this important pest of citrus
all over the world. 相似文献
76.
A series of experiments were conducted to examine the effect of 3-octylthio-1,1,1-trifluoro-2-propanone (OTFP) on growth, development, and behavior of the cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae), and the corn stalk borer, Sesamia nonagrioides (Lepidoptera: Noctuidae). The chemical behaved as an oviposition deterrent and, when added to the diet of the second-instar larvae of both insects, reduced diet consumption and growth, pupation, and adult emergence. Treatment of 100-5000 ng of the compound on fourth-instar larvae for 3-24 h, however, did not produce significant differences in the amount of diet ingested. Our results suggest that the effect of OTFP is long-lasting and that the inhibitor is not fully detoxified by the detoxification enzymes of the digestive tract of the insects. In behavioral assays, adult males which had been treated with the chemical at the larval stage were less attracted to the pheromone source than regular untreated males. When Sp. littoralis untreated females were used as the attractant source, treated males also displayed significantly fewer contacts with the cage-containing females than untreated or solvent-treated males. In the presence of treated females, only 27% of treated males successfully completed the flight in comparison to animals responding to solvent-treated females (54.5%). By contrast, when Se. nonagrioides females, whether they had been subjected or not to the treatment, were used as the attractant source, males were similarly attracted to them regardless of whether they had been treated or not at the larval stage. Analyses of gland extracts of Sp. littoralis treated females showed no difference from control insects in the qualitative or quantitative composition of the pheromone blend. The results obtained, in combination with other results previously reported by us (Riba, M.; Sans, A.; Bau, P.; Grolleau, G.; Renou, M.; Guerrero, A. J. Chem. Ecol. 2001, 27, 1879-1897), provide new and relevant information about the possible utility of these chemicals in future studies directed to the development of new approaches for pest control. 相似文献
77.
Development of Core Subset of Finger Millet Germplasm Using Geographical Origin and Data on 14 Quantitative Traits 总被引:6,自引:0,他引:6
Hari D. Upadhyaya C. L. L. Gowda R. P. S. Pundir V. Gopal Reddy Sube Singh 《Genetic Resources and Crop Evolution》2006,53(4):679-685
Finger millet [Eleusine coracana (L.) Gaertn.] is an important cereal food crop in Africa and South Asia. It is a hardy crop that can be grown in very diverse
environments from almost at sea level to about 2400 m.a.s.l. Finger millet has an excellent food value as its seeds contain
protein ranging from 7 to 14% and are particularly rich in methionine amino acid, iron, and calcium. Despite all these merits,
this crop has been neglected from the main stream of crop improvement research. One of the means to boost its production and
productivity is to enhance utilization of finger millet germplasm to breed superior varieties. Keeping this objective in view,
a core subset of finger millet germplasm (622 accessions) based on origin and data on 14 quantitative traits was developed
from the entire global collection of 5940 accessions held in the genebank at ICRISAT, Patancheru, India. The comparison of
means, variances, frequency distribution, Shannon-Weaver diversity index (H′) and phenotypic correlations indicated that the
core subset represents the entire collection. These tests indicated that sampling was optimal and the diversity has been captured
very well in the core subset. The correlation analysis indicated that panicle exsertion and longest finger length could be
given lower priority in the future germplasm evaluation work of finger millet. 相似文献
78.
Availability, fixation, and transformation of added P were studied in a 16-week incubation experiment with a Vertisol amended with farmyard manure in pots with 500 g soil each. P availability, as measured by Olsen P, decreased for up to 8 weeks with various rates of added P, when no manure was applied. In the presence of farmyard manure, P availability decreased during the first 6 weeks and then showed a considerable increase from the 8th week onwards. P fixation increased for up to 8 weeks with the rates of P in the absence of manure. With manure application, P fixation increased only during the first 6 weeks and thereafter decreased continuously. Thus the presence of farmyard manure shortened the period of P fixation and promoted its availability. After 16 weeks of incubation, when manure and fertilizer P were applied together, P was transformed into labile organic (NaHCO3–P), moderately labile organic P (NaOH-P), and calcium-bound inorganic P (HCl-P). When manure was not applied. P accumulated predominantly as labile inorganic (NaHCO3–P), moderately labile inorganic (NaOH-P), and inorganic HCl-P. The application of farmyard manure enriched long-term P fertility through NaHCO3–P and NaOH–P and a shortterm P supply as HCl-P. All fractions except inorganic NaOH-P showed good relationships with Olsen P. 相似文献
79.
Reddy MK Alexander-Lindo RL Nair MG 《Journal of agricultural and food chemistry》2005,53(23):9268-9273
The most abundant water soluble natural food colors are betacyanins and anthocyanins. Similarly, lycopene, bixin, beta-carotene, and chlorophyll are water insoluble colors. Pure betanin, bixin, lycopene, chlorophyll, beta-carotene, and cyanidin-3-O-glucoside were isolated from Beta vulgaris, Bixa orellana,Lycopersicum esculentum, Spinacia oleracea, Daucus carrota, and Prunus cerasus, respectively. These natural pigments, alone and in combination, were evaluated for their relative potencies against cyclooxygenase enzymes and tumor cell growth inhibition by using MCF-7 (breast), HCT-116 (colon), AGS (stomach), CNS (central nervous system), and NCI-H460 (lung) tumor cell lines. Among the colors tested, betanin, cyanidin-3-O-glucoside, lycopene, and beta-carotene inhibited lipid peroxidation. However, all pigments tested gave COX-1 and COX-2 inhibition and showed a dose-dependent growth inhibition against breast, colon, stomach, central nervous system, and lung tumor cells, respectively. The mixtures of these pigments were also evaluated for their synergistic effects and chemical interactions at various concentrations. The mixture of anthocyanin and betanin negated their efficacy in the cell growth inhibitory assay and did not enhance the COX enzyme inhibitory activity. This is the first report of a comparative evaluation and the impact on biological activities of these pigments alone and in combination. 相似文献
80.