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Pain A Renauld H Berriman M Murphy L Yeats CA Weir W Kerhornou A Aslett M Bishop R Bouchier C Cochet M Coulson RM Cronin A de Villiers EP Fraser A Fosker N Gardner M Goble A Griffiths-Jones S Harris DE Katzer F Larke N Lord A Maser P McKellar S Mooney P Morton F Nene V O'Neil S Price C Quail MA Rabbinowitsch E Rawlings ND Rutter S Saunders D Seeger K Shah T Squares R Squares S Tivey A Walker AR Woodward J Dobbelaere DA Langsley G Rajandream MA McKeever D Shiels B Tait A Barrell B Hall N 《Science (New York, N.Y.)》2005,309(5731):131-133
Theileria annulata and T. parva are closely related protozoan parasites that cause lymphoproliferative diseases of cattle. We sequenced the genome of T. annulata and compared it with that of T. parva to understand the mechanisms underlying transformation and tropism. Despite high conservation of gene sequences and synteny, the analysis reveals unequally expanded gene families and species-specific genes. We also identify divergent families of putative secreted polypeptides that may reduce immune recognition, candidate regulators of host-cell transformation, and a Theileria-specific protein domain [frequently associated in Theileria (FAINT)] present in a large number of secreted proteins. 相似文献
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Meister S Plouffe DM Kuhen KL Bonamy GM Wu T Barnes SW Bopp SE Borboa R Bright AT Che J Cohen S Dharia NV Gagaring K Gettayacamin M Gordon P Groessl T Kato N Lee MC McNamara CW Fidock DA Nagle A Nam TG Richmond W Roland J Rottmann M Zhou B Froissard P Glynne RJ Mazier D Sattabongkot J Schultz PG Tuntland T Walker JR Zhou Y Chatterjee A Diagana TT Winzeler EA 《Science (New York, N.Y.)》2011,334(6061):1372-1377
Most malaria drug development focuses on parasite stages detected in red blood cells, even though, to achieve eradication, next-generation drugs active against both erythrocytic and exo-erythrocytic forms would be preferable. We applied a multifactorial approach to a set of >4000 commercially available compounds with previously demonstrated blood-stage activity (median inhibitory concentration < 1 micromolar) and identified chemical scaffolds with potent activity against both forms. From this screen, we identified an imidazolopiperazine scaffold series that was highly enriched among compounds active against Plasmodium liver stages. The orally bioavailable lead imidazolopiperazine confers complete causal prophylactic protection (15 milligrams/kilogram) in rodent models of malaria and shows potent in vivo blood-stage therapeutic activity. The open-source chemical tools resulting from our effort provide starting points for future drug discovery programs, as well as opportunities for researchers to investigate the biology of exo-erythrocytic forms. 相似文献
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Lukumoni Buragohain Trilok Nanda Arnab Ghosh Mayukh Ghosh Rajesh Kumar Sunil Kumar Sambhu Sharan Gupta Arpita Bharali Ashok K. Mohanty Inderjeet Singh Ashok Kumar Balhara 《Animal Science Journal》2017,88(8):1189-1197
Improper or delayed pregnancy diagnosis has significant impact over animal production, particularly in buffaloes which inherently suffer from several reproductive inefficiencies. Thus the present study has undertaken to identify serum protein markers pertaining to early pregnancy diagnosis in buffaloes. Serum samples were collected from 10 pregnant Murrah Buffalo heifers at weekly intervals from days 0‐35 post‐artificial insemination and from 12 inseminated non‐pregnant cyclic buffalo heifers on days 0, 7, 14 and 21. Two‐dimensional gel electrophoresis and densitometric analysis revealed the presence of five protein spots showing average density fold change of ≥4 during early pregnancy. Mass spectrometry analysis identified these up‐regulated proteins as anti‐testosterone antibody light chain, apolipoprotein A‐II precursor, serum amyloid A, cytokeratin type II, component IV isoform 1, which are have established roles in embryogenesis, but over‐expression of the fifth identified protein immunoglobulin lambda light chain in pregnancy has been elucidated as a novel finding in the current study. Further, with bioinformatics analysis, potential antigenic B‐cell epitopes were predicted for all these five proteins. An antibody cocktail‐based approach involving antibodies against all these five up‐regulated entire proteins or their epitopes could be developed for early detection of pregnancy in buffaloes. © 2016 Japanese Society of Animal Science 相似文献
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Dudakov JA Hanash AM Jenq RR Young LF Ghosh A Singer NV West ML Smith OM Holland AM Tsai JJ Boyd RL van den Brink MR 《Science (New York, N.Y.)》2012,336(6077):91-95
Endogenous thymic regeneration is a crucial function that allows for renewal of immune competence after stress, infection, or immunodepletion. However, the mechanisms governing this regeneration remain poorly understood. We detail such a mechanism, centered on interleukin-22 (IL-22) and triggered by the depletion of CD4(+)CD8(+) double-positive thymocytes. Intrathymic levels of IL-22 were increased after thymic insult, and thymic recovery was impaired in IL-22-deficient mice. IL-22, which signaled through thymic epithelial cells and promoted their proliferation and survival, was up-regulated by radio-resistant RORγ(t)(+)CCR6(+)NKp46(-) lymphoid tissue inducer cells after thymic injury in an IL-23-dependent manner. Administration of IL-22 enhanced thymic recovery after total body irradiation. These studies reveal mechanisms of endogenous thymic repair and offer innovative regenerative strategies for improving immune competence. 相似文献
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Ashis Maity Jyotsana Sharma Ananta Sarkar Amarja K. More Ram K. Pal Vinayak P. Nagane Arnab Maity 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(4):923-937
Pomegranate bacterial blight disease caused by Xanthomonas axonopodis pv. punicae is one of the most serious threat to the pomegranate industry in India. So far, the chemicals used to control this pathogen alone are less effective. In this article, an attempt was made to supplement chemical control through improving the systemic acquired resistance of plant using nutrients such as nitrogen (N) and salicylic acid (SA) as elicitor. The study revealed that foliar application of SA at the rate of 300 ppm increased above-ground dry mass of plant by 64.97%, improved chlorophyll content of leaves and resulted higher concentration of macro-elements and micro-elements, particularly Mn and Zn, in leaves. It also significantly increased the activities of nitrate reductase (NR), superoxide dismutase (SOD), catalase and peroxidase. However, the maximum response of SA was recorded when it was sprayed after soil application of N at 100% of the recommended dose. As a consequence, this combination recorded the least bacterial blight infection with average severity of 11.58% followed by without-N application under challenge inoculation of the pathogen. However, sustainable production cannot be realized without N application. The integration of this strategy for activation of defence mechanism of plant with the bactericide streptocycline resulted in further reduction of bacterial blight disease incidence and severity under field condition to very low levels (3.84% and 18.57% respectively). 相似文献