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21.
Phytophthora niederhauserii, P. pisi, P. sojae and P. vignae are closely related species that are pathogenic to various legume plants. While P. sojae and P. vignae are reported to specifically infect soybean and cowpea, respectively, P. pisi is reported to attack pea and faba bean. Phytophthora niederhauserii is considered to have a broad host range. Zoospores of some Phytophthora species are chemotactically attracted to the isoflavones that are secreted by their host plants. The focus of the current study was to determine the chemotaxic behaviour of zoospores from closely related legume‐root infecting Phytophthora species and to investigate the correlation, if any, to host preference as determined by greenhouse pathogenicity tests. The results showed that P. sojae and P. vignae were attracted to the non‐soybean isoflavone prunetin as well as to the soybean isoflavones genistein and daidzein, which is in contrast with their host specificity on soybean and cowpea, respectively. On the other hand, P. pisi and P. niederhauserii were only attracted to prunetin, previously reported to be produced by pea, but not to the isoflavones associated with the non‐host soybean. The lack of responsiveness to genistein and daidzein in P. pisi may represent a recent adaptation to the host specialization towards pea. However, the affinity of P. niederhauserii to prunetin shows that this trait can also be present in taxa not specifically associated with legume hosts.  相似文献   
22.
Research has shown that fresh fish is an extremely perishable food as compared to other food commodities. In this study, quality deterioration during storage of golden grey mullet (GGM) iced for 0, 6, and 12 h after landing was monitored. Microbiological indices (total viable count, TVC; psychrophilic counts, PTC; lactic acid bacteria, LAB; and Enterobacteriaceae counts, EBC), chemical parameters (pH; total volatile basic nitrogen, TVB-N; peroxide value, PV; thiobarbituric acid, TBA; and free fatty acids, FFA) values increased over the preicing duration. Delayed icing did not lead to significant increases (p > 0.05) in TVC, PTC, LAB, and EBC throughout the period of storage. All chemical indices increased during storage (p < 0.05). This study showed that sensorial analysis of GGM correlated well with microbiological analysis. Results of this study based on the microbiological and raw fish sensorial data indicated that the shelf life of GGM stored in ice immediately after catch was approximately 10–11 days, while the 6- and 12-h delays in icing shortened the shelf life to approximately 6–7 and 2–3 days, respectively.  相似文献   
23.
Effect of dietary tryptophan (TRP) on copper toxicity was investigated in common carp, Cyprinus carpio. Fish were randomly distributed into two triplicate treatments (control and TRP) and fed control (TRP?=?3.1?g?kg(-1)) or TRP (TRP?=?8.1?g?kg(-1)) diets over a 2-week period. Then, both treatments were subjected to 10?mg?l(-1) copper sulfate over a 7-day period. Mortality and serum cortisol, glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), sodium, potassium, and chloride levels were recorded at 0, 24, 72, 120, and 168?h post-copper exposure. There was no mortality in the TRP group, whereas the control group mortality was started at 120?h post-copper exposure (21.7?±?7.6?%) and reached 61.7?±?10.4?% at the end of the experiment. Cortisol, glucose, ALT, and AST levels significantly (P?相似文献   
24.
Oocyte maturation in fish is a hormonally regulated process. In the light of long-term oocyte maturation in beluga, the aim of this research was to study the estrogenic effects of different concentrations of soy dietary genistein (GE) and equol (EQ) on the growth performance and ovary development in farmed female Huso huso. Fish were fed with concentrations 0 (control), 0.2, 0.4, 0.8 and 1.6 g of EQ and GE per kg of isoproteic (CP 45 %) and isoenergetic (19.5 MJ/kg) diets during a year. Blood samples and ovary biopsies were collected from each fish seasonally. The main results of the present experimentation are that growth performance was not affected significantly both in GE and EQ (P > 0.05). EQ at concentration 0.4 g/kg had more estrogenic effects than other concentrations of EQ and GE in beluga so that 64 % of fish were matured sexually. Some reproductive indices such as oocyte diameter, testosterone (T) and 17β-estradiol (E2) increased significantly at EQ 0.4 g/kg at the end of experiment (P < 0.05), while 17α-hydroxy progesterone level (17α-OHP) showed no significant changes at all concentrations. Biochemical indices such as calcium, phosphorous and cholesterol increased at GE concentrations, but decreased at EQ concentrations similarly at the end of experiment. There was a negative relationship between plasma phosphorous and alkaline phosphatase enzyme levels. Based on results, EQ at concentration 0.4 g/kg improved oocyte development more than the other concentrations of GE and EQ, and therefore, it can be used as an additive to diets for inducing ovary development in this species.  相似文献   
25.

Purpose

The main objective of this study was to examine the potential of using hyperspectral image analysis for prediction of total carbon (TC), total nitrogen (TN) and their isotope composition (δ13C and δ15N) in forest leaf litterfall samples.

Materials and methods

Hyperspectral images were captured from ground litterfall samples of a natural forest in the spectral range of 400–1700 nm. A partial least-square regression model (PLSR) was used to correlate the relative reflectance spectra with TC, TN, δ13C and δ15N in the litterfall samples. The most important wavelengths were selected using β coefficient, and the final models were developed using the most important wavelengths. The models were, then, tested using an external validation set.

Results and discussion

The results showed that the data of TC and δ13C could not be fitted to the PLSR model, possibly due to small variations observed in the TC and δ13C data. The model, however, was fitted well to TN and δ15N. The cross-validation R2 cv of the models for TN and δ15N were 0.74 and 0.67 with the RMSEcv of 0.53% and 1.07‰, respectively. The external validation R2 ex of the prediction was 0.64 and 0.67, and the RMSEex was 0.53% and 1.19 ‰, for TN and δ15N, respectively. The ratio of performance to deviation (RPD) of the predictions was 1.48 and 1.53, respectively, for TN and δ15N, showing that the models were reliable for the prediction of TN and δ15N in new forest leaf litterfall samples.

Conclusions

The PLSR model was not successful in predicting TC and δ13C in forest leaf litterfall samples using hyperspectral data. The predictions of TN and δ15N values in the external litterfall samples were reliable, and PLSR can be used for future prediction.
  相似文献   
26.

Purpose

This study aimed to assess the effects of biochar on improving nitrogen (N) pools in mine spoil and examine the effects of elevated CO2 on soil carbon (C) storage.

Materials and methods

The experiment consisted of three plant species (Austrostipa ramossissima, Dichelachne micrantha, and Lomandra longifolia) planted in the N-poor mine spoil with application of biochar produced at three temperatures (650, 750, and 850 °C) under both ambient (400 μL L?1) and elevated (700 μL L?1) CO2. We assessed mine spoil total C and N concentrations and stable C and N isotope compositions (δ13C and δ15N), as well as hot water extractable organic C (HWEOC) and total N (HWETN) concentrations.

Results and discussion

Soil total N significantly increased following biochar application across all species. Elevated CO2 induced soil C loss for A. ramossissima and D. micrantha without biochar application and D. micrantha with the application of biochar produced at 750 °C. In contrast, elevated CO2 exhibited no significant effect on soil total C for A. littoralis, D. micrantha, or L. longifolia under any other biochar treatments.

Conclusions

Biochar application is a promising means to improve N retention and thus, reduce environmentally harmful N fluxes in mine spoil. However, elevated CO2 exhibited no significant effects on increasing soil total C, which indicated that mine spoil has limited potential to store rising atmospheric CO2.
  相似文献   
27.
The present study describes the preparation and characterization of montmorillonite-urea nanocomposites (Mt-Ur) using aqueous suspension technique at various stirring times and different ratio of montmorillonite to urea (Mt/Ur) via an impure and domestic montmorillonite (Mt), without the application of any chemical reagents and high-energy-demand process (environmentally friendly). The intercalation of urea into Mt interlayer was clearly demonstrated by a significant expansion of d001 spacing (interlayer space of Mt) from 1.23 to 1.71 nm which has not yet been reported by aqueous suspension technique. Analyses performed by Fourier transform-infrared spectroscopy (FT-IR), Thermogravimetric analysis (TGA) and X-ray diffraction (XRD) also confirmed the effectiveness of this simple process to intercalate the urea into clay lamella. The release pattern demonstrated that the nanocomposite had a slow-release behaviour for urea dissolution. The results also suggested that the Mt type applied in the current study, in a 1:20 Mt/Ur ratio and stirred for 1 h, possessed desirable ecological and economic efficiency in the production of slow-release urea fertilizer due to the application of the impure and domestic clay which is of very low-cost and eco-friendly. Nevertheless, urea was fully intercalated into the interlayer of clay by a simple technique and with a good slow-release behaviour.  相似文献   
28.
Four-day-old seedlings of spring wheat (Triticum aestivum L. cv. Marvdasht) can tolerate severe dehydration and recover after re-watering. In order to clarify tolerance mechanism, in a completely randomized design, the seedlings were exposed to drought by complete water cessation for 10 and 20 days, followed by re-watering for 3 and 7 days. Upon dehydration, the activity of α-amylase increased whereas growth parameters; photosynthetic pigments; and the activities of lipoxygenase, indole acetic acid (IAA) oxidase, and ascorbate peroxidase (APX) decreased. The activity of APX and the expression of its gene, however, increased after re-watering. Prolonged dehydration was also accompanied by increase in sucrose, soluble proteins, fructans, and certain amino acids, e.g. proline, glutamine, and valine. The results indicated that during dehydration, 4-day-old wheat seedlings shifted their energy expenditure from basal metabolism to withstand water deficiency via lowering the activity of membrane-damaging enzymes and enhancing the production of osmoprotectants.  相似文献   
29.
30.
The main aim of this study was to examine the effects of a polyculture system on the control of the external parasites of western white shrimp, Litopenaeus vannamei. To this end, the western white shrimp postlarvae (PLs) were stocked in nine earthen ponds (600 m2) at a density of 20 PLs m?2 and reared for 4 months. After 40 days of shrimp stocking, Mullets, Mugil cephalus, were stocked at various densities including: control (0 fish/100 m2 pond), treatment 1 (T1: 2 fish/100 m2 pond) and treatment 2 (T2: 4 fish/100 m2 pond). Over the course of the experiment, the external parasites of shrimps were investigated by the preparation of a wet mount from the gill tissue. Based on the obtained results, totally two genera of protozoan parasites, i.e. Zoothamnium sp. and Epistylis sp., were identified over the course of the experiment. In all experimental groups, the incidence and abundance of Zoothamnium sp. was significantly higher than Epistylis sp. (< 0.05). Also, mean incidence per cent and mean abundance of Zoothamnium sp. and Epistylis sp. were significantly lower in the polyculture treatments (T1 and T2) compared to the monoculture group (control) (P < 0.05). Throughout this experiment, the total organic matter (TOM %) content of the bottom sediments and biological oxygen demand (BOD5 mg L?1) of water samples in the polyculture ponds were significantly lower than the monoculture group (P < 0.05). In contrast, the polyculture ponds had a higher concentration of water dissolved oxygen (O2 mg L?1) compared to the monoculture (P < 0.05). In conclusion, our results show that mullet as a secondary farmed species can reduce indirectly the parasitic pollution of western white shrimp probably through reducing the total organic matters in water and sediments and improving the water quality parameters.  相似文献   
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