Die Rolle der mikrobiellen Biomasse und des mineralisch fixierten Ammoniums bei den Stickstoff-Transformationen in niedersächsischen Löß-Ackerböden unter Winter-Weizen. II. Umsetzung von 15N-markiertem Stickstoff     

Rolf Nieder  Astrid Willenbockel  Elisabeth Neugebauer  Paul Widmer  Jrg Richter 《植物养料与土壤学杂志》1995,158(5):477-484

Significance of microbial biomass and mineral fixed ammonium with respect to the nitrogen transformations in loess soils of Niedersachsen during the growing season of winter wheat. II. ¹⁵N-turnover Field experiments 1988/89 on a fallow plot of the southern Niedersachsen loess area with straw application (δ 10 t · ha^?1, homogeneously incorporated by hand) yielded an increase in microbial biomass-N (N_mic) by 60 kg N · ha^?1 · 30 cm^?1 until March 1989 and further 40 kg N · ha^?1, till May which was almost completely remobilized until harvest. For a cropped plot (with winter wheat and 10 t · ha^?1 straw incorporation), N immobilization was of similar magnitude. Up to 18% of the applied ¹⁵N-fertilizer (185 kgN · ha^?1) were microbially immobilized. In contrast to 1988/89, no significant mass change of N_mic occurred in 1991 due to straw application (δ 10t · ha^?1). Variations in the amount of N_mic were nearly independent on the treatment (crop, with 140 kg fertilizer-N · ha^?1 or without N-fertilizer, respectively; fallow plot without fertilizer-N) within a range of 225-400 kg N · ha^?1 · 30 cm^?1. Directly after N-application (each 70 kg N · ha^?1 in March and in May), up to 100% of the fertilizer-N were assimilated by the microbes. Subsequently, remobilization of the immobilized nitrogen occurred within 2 (in March) or 6 weeks (in May), respectively. Simultaneously, organic soil-N was mineralized after each N-application and minerally fixed for us biggest part. Between March and June, the fixed NH₄+ decreased by about 112 kg · ha^?1 · 30 cm^?1.  相似文献   

Analysis of early lipid oxidation in smoked, comminuted pork or poultry sausages with spices     

Olsen E  Vogt G  Veberg A  Ekeberg D  Nilsson A 《Journal of agricultural and food chemistry》2005,53(19):7448-7457

Dynamic headspace/gas chromatography-mass spectrometry (GC-MS), front-face fluorescence spectroscopy, and a gas-sensor array technique (electronic nose) have previously detected lipid oxidation in pork back fat or mechanically recovered poultry meat earlier than or at the same time as a sensory panel. The present study was focused on measurement of early lipid oxidation in a more complicated product (freeze-stored, smoked sausages with spices). During the storage time, formation of components contributing to rancid odor and flavor (e.g., hexanal and 1-penten-3-ol) could be monitored with dynamic headspace/GC-MS. The GC-MS data also showed a decrease in 2-furancarboxaldehyde, which could indicate loss of Maillard type components often associated with acidic or meat odor and flavor. The fluorescence spectra were difficult to interpret, probably due to the simultaneous influence from increasing levels of lipid oxidation products and loss of fluorescent Maillard or spice components. The gas-sensor array responses were dominated by signals from, e.g., spice and smoke compounds.  相似文献   

Analysis of the early stages of lipid oxidation in freeze-stored pork back fat and mechanically recovered poultry meat     

Olsen E  Vogt G  Ekeberg D  Sandbakk M  Pettersen J  Nilsson A 《Journal of agricultural and food chemistry》2005,53(2):338-348

An analytical method that can detect low levels of oxidation in food earlier than a sensory panel would be a valuable tool for food manufacturers as well as research institutes. Two model matrixes, pork back fat and mechanically recovered poultry meat (MRPM), were freeze-stored in air at -20 degrees C for 26 weeks. Peroxide value, thiobarbituric acid reactive substances, volatiles analyzed with dynamic headspace gas chromatography-mass spectrometry (GC-MS) and a gas-sensor array technique (electronic nose), chemiluminescence, and front-face fluorescence were evaluated against sensory analysis with regard to detection of early oxidation and correlation with sensory data. Fluorescence and GC-MS could detect oxidative changes in pork back fat earlier than the sensory panel and the electronic nose at the same time. The three methods were highly correlated with sensory attributes (r = 0.8-0.9). GC-MS gave the best results with regard to detection of small oxidative changes in MRPM.  相似文献   

Prevalence and characterization of hypoadrenocorticism in dogs with signs of chronic gastrointestinal disease: A multicenter study     

Christina Hauck  Silke S. Schmitz  Iwan A. Burgener  Astrid Wehner  Reto Neiger  Barbara Kohn  Thomas Rieker  Sven Reese  Stefan Unterer 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(4):1399-1405

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Prevalence of specific IgG-antibodies against Toxoplasma gondii in domestic turkeys determined by kinetic ELISA based on recombinant GRA7 and GRA8     

Koethe M  Pott S  Ludewig M  Bangoura B  Zöller B  Daugschies A  Tenter AM  Spekker K  Bittame A  Mercier C  Fehlhaber K  Straubinger RK 《Veterinary parasitology》2011,180(3-4):179-190

The protozoan parasite Toxoplasma (T.) gondii is one of the most common zoonotic infectious agents worldwide. Besides its sexual reproduction in cats, T. gondii can also infect a wide spectrum of other warm-blooded animals. These include animals used for human consumption such as pigs or chickens. Nevertheless, the role of turkeys for the epidemiology of T. gondii infections has not been studied thoroughly. We have established a kinetic ELISA (KELA) for the detection of T. gondii-specific IgG antibodies in turkey serum samples. The test is based on the recombinant dense granule antigens GRA7 and GRA8. These proteins were used as an antigen mixture at a concentration of 0.13 μg per well. The overall sensitivity of the assay was between 92.6% and 100% and the specificity ranged from 78.1% to 100%, depending on the method used to calculate these parameters. Using this KELA we examined 1913 turkey serum samples from 14 turkey farms from different areas of Germany. From these sera, 387 produced a signal in the KELA, corresponding to a true seroprevalence of up to 20.2%. The seropositivity rate in individual fattening cycles at individual farms ranged from 0.0% to 77.1%, whereas the rates were highly variable within the individual farms and individual fattening cycles. Consequently, conditions of animal husbandry could not be associated with particular seroprevalence rates. Although seropositivity cannot be linked directly to infectious tissue cysts in the muscle tissue of commercially produced turkey meat, we state that there is a potential risk of being infected by consuming turkey meat products that were not heat treated.  相似文献   

A unique novel reptilian paramyxovirus, four atadenovirus types and a reovirus identified in a concurrent infection of a corn snake (Pantherophis guttatus) collection in Germany     

Abbas MD  Marschang RE  Schmidt V  Kasper A  Papp T 《Veterinary microbiology》2011,150(1-2):70-79

In 2009, 26 clinical samples (organs and oral/cloacal swabs) from a total of 24 corn snakes (Pantherophis guttatus) from a single owner were sent to our laboratory to be tested for the presence of viruses. Paramyxoviruses (PMV), adenoviruses (AdV) and reoviruses were detected by RT-PCR, PCR and virus isolation methods. Three snakes were infected with all three viruses at the same time, while two other snakes had a double infection (PMV and reo, AdV and reo) and nine other snakes had a single infection with any of the three viruses. No viruses were detected in 10 animals. All isolated reoviruses were identical to one another and to the reptilian orthoreovirus isolate 55-02 in the partial RNA dependent RNA polymerase (RDRP) gene sequence. AdV partial polymerase sequences represented four different types, one of which was first described here: most similar to SnAdV-1, while the other three were identical to known types: SnAV-1, -2 and -3. However, the detected single PMV differed distinctly from described reptile PMV and was a new type. According to partial L gene, HN gene and U gene sequences it may be the first described representative of a third squamatid PMV cluster: "group C" within the proposed reptilian PMV genus "Ferlavirus". Nucleotide identity values for the L gene of the new PMV compared to group A viruses range between 76.5 and 80.3%, and between 80.5 and 81.2% compared to group B viruses. For the HN gene, these values were similar: 78.2-80% (A) and 79.9-80.5% (B) and somewhat lower for the U gene: 72.7-75.4% (A) and 69.7-70% (B). No reports on the prevalence of concurrent viral infection in captive snake populations have been published so far. The possibility of concurrent infection with several different viruses and subsequent consequences for animal health should be kept in mind when testing reptile samples for viruses.  相似文献   

Extended-spectrum beta-lactamases-producing gram-negative bacteria in companion animals: action is clearly warranted!     

Ewers C  Grobbel M  Bethe A  Wieler LH  Guenther S 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(3-4):94-101

Extended-spectrum beta-lactamases (ESBL)-producing Gram-negative bacteria pose a serious threat to Public Health in human medicine as well as increasingly in the veterinary context worldwide. Several studies reported the transmission of zoonotic multidrug resistant bacteria between food-producing animals and humans, whilst the contribution of companion animals to this scenario is rather unknown. Within the last decades a change in the social role of companion animals has taken place, resulting in a very close contact between owners and their pets. As a consequence, humans may obtain antimicrobial resistant bacteria or the corresponding resistance genes not only from food-producing animals but also via close contact to their pets.This may give rise to bacterial infections with limited therapeutic options and an increased risk of treatment failure. As beta-lactams constitute one of the most important groups of antimicrobial agents in veterinary medicine, retaliatory actions in small animal and equine practices are urgently needed. This review addresses the increasing burden of extended-spectrum beta-lactam resistance among Enterobacteriaceae isolated from companion animals. It should emphasize the urgent need for the implementation of antibiotic stewardship as well as surveillance and monitoring programs of multi resistant bacteria in particular in view of new putative infection cycles between humans and their pets.  相似文献   

Gene expression and hormonal regulation of adiponectin and its receptors in bovine mammary gland and mammary epithelial cells     

Yoshihisa OHTANI  Tomo YONEZAWA  Sang‐Houn SONG  Tatsuyuki TAKAHASHI  Astrid ARDIYANTI  Katsuyoshi SATO  Akihiko HAGINO  Sang‐gun ROH  Kazuo KATOH 《Animal Science Journal》2011,82(1):99-106

Although the functions of adiponectin, a differentiated adipocyte‐derived hormone, in regulating glucose and fatty acid metabolism are regulated by two subtypes of adiponectin receptors (AdipoRs; AdipoR1 and AdipoR2), those in ruminants remain unclear. Therefore we examined the messenger RNA (mRNA) expression levels of adiponectin and its receptors in various bovine tissues and mammary glands among different lactation stages, and the effects of lactogenic hormones (insulin, dexamethasone and prolactin) and growth hormone (GH) on mRNA expression of the AdipoRs in cultured bovine mammary epithelial cells (BMEC). AdipoRs mRNAs were widely expressed in various bovine tissues, but adiponectin mRNA expression was significantly higher in adipose tissue than in other tissues. In the mammary gland, although adiponectin mRNA expression was significantly decreased at lactation, AdipoR1 mRNA expression was significantly higher at peak lactation than at the dry‐off stage. In BMEC, lactogenic hormones and GH upregulated AdipoR2 mRNA expression but did not change that of AdipoR1. In conclusion, adiponectin and its receptor mRNA were expressed in various bovine tissues and the adiponectin mRNA level was decreased during lactation. These results suggest that adiponectin and its receptors ware changed in mammary glands by lactation and that AdipoRs mRNA expression was regulated by different pathways in BMEC.  相似文献   

Impervious and pervious pavements increase soil CO₂ concentrations and reduce root production of American sweetgum (Liquidambar styraciflua)     

Bhavana Viswanathan  Astrid Volder  W. Todd Watson  Jacqueline A. Aitkenhead-Peterson 《Urban Forestry & Urban Greening》2011,10(2):133

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Optimization, in-house validation, and application of a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method for the quantification of selected polyphenolic compounds in leaves of grapevine (Vitis vinifera L.)     

Schoedl K  Forneck A  Sulyok M  Schuhmacher R 《Journal of agricultural and food chemistry》2011,59(20):10787-10794

Polyphenols in grapevine can be constitutive or induced, depending upon cultivar, plant organ, and environmental influences. The aim of the presented work was to develop and optimize a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to study the pattern and amount of selected polyphenols in leaves of Vitis vinifera L. The method is simple and does not require any sample cleanup. It covers representative metabolites of the structure classes cinnamic acids, flavonoids, and stilbenes and enables the simultaneous separation and quantification of 13 polyphenols within 9 min at concentration levels between 0.1 and 3 μg/g. We present the method performance characteristics and its application to the quantification of polyphenols in grapevine leaves of the cultivars Riesling and Pinot noir. A total of 7 of 13 target polyphenols were detected at concentrations above the limits of quantification. Interestingly, instead of the expected trans-resveratrol, the investigated leaf samples of both cultivars contained cis-resveratrol-3-O-glucoside. The measurements also showed that Riesling leaves tended to contain higher concentrations of the selected polyphenols than Pinot noir. In view of its intended future use, the developed method has been shown to be a powerful and fast tool to study polyphenols in grapevine leaves subjected to environmental stress conditions.  相似文献