Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs     

Siqueira AK  Ribeiro MG  Leite Dda S  Tiba MR  Moura Cd  Lopes MD  Prestes NC  Salerno T  Silva AV 《Research in veterinary science》2009,86(2):206-210

The aim of this study was to compare the prevalence of virulence genes in 158 Escherichia coli strains isolated from 51 clinical cases of UTIs, 52 of pyometra and from 55 fecal samples from healthy dogs by PCR. papC was found in 12 (23.5%) strains isolated from UTIs, 19 (36.5%) from pyometra and 10 (18.2%) from feces. papGII was observed in 3 (5.8%) strains from pyometra, and papGIII in 10 (19.6%) from UTIs, 15 (28.8%) from pyometra and 9 (16.4%) from feces. sfaS was detected in 22 (43.1%) strains from UTIs, 24 (46.1%) from pyometra and 19 (34.5%) from feces. hlyA was observed in 17 (33.3%) strains from UTIs, 18 (34.6%) from pyometra and 7 (12.7%) from feces, while cnf-1 was detected in 11 (21.6%) from UTIs, 21 (40.4%) from pyometra and 9 (16.4%) from feces. iucD was observed in 12 (23.5%) strains from UTIs, 9 (17.3%) from pyometra and 1 (1.8%) from feces. usp was found 17 (33.3%) isolates from UTIs and 36 (69.9%) from pyometra.  相似文献   

Phenotypic and genotypic characterization of Prototheca zopfii in a dog with enteric signs     

Mrcio Garcia Ribeiro  Marconi Rodrigues de Farias  Uwe Roesler  Katja Roth  Sabrina Mari Rodigheri  Marco Antonio Ostrowsky  Tatiana Salerno  Amanda Keller Siqueira  Marta Catarina Fernandes 《Research in veterinary science》2009,87(3):479-481

This is a case report of enteric protothecosis caused by Prototheca zopfii in an eight-year-old male mixed breed dog with a history of chronic bloody diarrhea, loss of appetite and weight loss. Algae were isolated from rectal scrapings in defibrinated sheep blood agar and dextrose Sabouraud agar. Cytological evaluation showed the presence of globular and cylindrical organisms with a defined capsule and variable number of endospores, characteristic of the genus Prototheca, in the rectum of the animal. Scanning electron microscopy of P. zopfii strains at different development stages confirmed the diagnosis of algal infection. Molecular identification using a conserved 18S rDNA gene sequence determined that the strain belonged to genotype 2. This report describes success on treatment of canine protothecosis, diagnosed based on clinical, cytological, microbiological, scanning electron microscopy and genotypical findings.  相似文献   

Does a Synoptic Classification Indicate the NO x Pollution Potential? The Case of the Metropolitan Area of Tel Aviv, Israel     

Hadas Saaroni  Baruch Ziv  Tatiana Uman 《Water, air, and soil pollution》2010,207(1-4):139-155

This study examines the synoptic conditions controlling NO _x pollution in the metropolitan area of Tel Aviv, using a semi-objective synoptic classification for the eastern Mediterranean. A day in which NO _x concentration exceeded the Israeli standard in ≥1 of the seven monitoring stations was defined an “exceeding day” and in ≥5 as an “extensive exceeding day”. For 1998–2004, 19% and 3% of the days were found exceeding and extensive exceeding days, respectively, over 85% of them in the winter months, November–March. The inter-annual variation in the occurrence of the synoptic types was found to explain 72% of the variations in the number of exceeding days. A significant negative trend in the occurrence of types with high pollution potential explained the decrease of 10% per year in the number of exceeding days during 1998–2004. The Red Sea Trough, though being cyclonic system, contributed 51% of the exceeding days, while highs, though being more frequent, contributed only 35%. The “pollution potential” of a synoptic type was defined as the percentage of exceeding days belonging to this type. The majority of synoptic types with the highest pollution potential were cyclonic, most being the Red Sea Trough with western axis, with 82% potential. Our findings indicate that the identity of the synoptic system as cyclonic or anticyclonic is not the key factor for the pollution potential in the study region, but rather, the ambient atmospheric conditions they induce, i.e., high temperatures, static stability, and weak easterly offshore flow. Local processes are the direct cause of the pollution and that the role of the synoptic conditions is to enable, or even to reinforce, the supportive meso-scale processes. This study is a first step in downscaling synoptic features to local NO _x pollution potential, constituting a basis for alarming against pollution events, based on the predicted synoptic conditions.  相似文献   

Acute pasteurellosis in fallow deer, cattle and pigs in a region of Eastern Germany     

Soike D  Schulze C  Kutzer P  Ewert B  van der Grinten E  Schliephake A  Ewers C  Bethe A  Rau J 《Berliner und Münchener tier?rztliche Wochenschrift》2012,125(3-4):122-128

Hemorrhagic septicaemia, an acute disease caused by P multocida capsular type B which is rarely detected in Europe, caused considerable losses in fallow deer, cattle and pigs within a region along the border of the federal states Brandenburg and Saxony-Anhalt in the summer of 2010. Clinical appearances and diagnostic findings are presented and possible triggering influences discussed. Pasteurella multocida capsular type B has not been cultivated from clinically healthy cattle and pigs of the region. Examination of fallow deer and roe deer in the region revealed the presence of singular carriers, which may act as a source of clinical infections.  相似文献   

Effects of Thymus vulgaris L. as feed additive in piglets and against haemolytic E. coli in vitro     

Jugl-Chizzola M  Spergser J  Schilcher F  Novak J  Bucher A  Gabler C  Hagmüller W  Zitterl-Eglseer K 《Berliner und Münchener tier?rztliche Wochenschrift》2005,118(11-12):495-501

The aim of the study was to test Thymi herba (1.66% v/w essential oil with 39% p-cymene and 32% thymol) in the rearing period of piglets as feed additive. Therefore, two feeding trials were performed with piglet groups ranging from 17 to 22 animals each. Either 10 g of Thymi herba/kg feed (Thymi herba group), 10 mg flavophospholipol/kg feed (flavophospholipol group) or nothing (control group) was added to the animals' feed. No significant differences in the performance parameter daily weight gain among any groups were recorded. No differences concerning feed efficiency or isolation of haemolytic E. coli serotypes were shown. In addition, the antibacterial activity of the essential oil of Thymi herba against 39 haemolytic E. coli isolates from the same weaners was investigated in vitro by disk diffusion, minimum inhibitory concentration and bactericidal concentration testing. In contrast to the feeding results, the essential oil of the thyme batch fed showed antibacterial activity against all haemolytic E. coli investigated. This interesting antibacterial potential of Thymi herba prompts further investigations as to its value as feed additive.  相似文献   

Immunohistochemical identification of lymphatic vessels in the periodontium of equine cheek teeth     

Staszyk C  Duesterdieck KF  Gasse H  Bienert A 《Journal of veterinary dentistry》2005,22(4):227-232

Immunohistochemical detection of lymphatic capillaries was performed in the periodontium of maxillary and mandibular cheek teeth from 6 horses (aged 3-23 years). Tissue sections of the periodontium were taken at 4 different horizontal levels along the long axis of the tooth. The specimens were processed for immunoreaction with anti-Prox1, in order to distinguish lymphatic endothelium from blood vascular endothelium. Lymphatic vessels were detected in all periodontal tissues except for the dental cementum. Lymphatic capillaries were most densely distributed in the gingiva compared to other tissues of the periodontium. Lymphatic capillaries were found most consistently in samples taken from the gingival and subgingival regions in all horses examined. Within these levels, the gingiva as well as the spongiosa of the maxillary and mandibular bone had the greatest incidence of lymphatic vessels. Considering the distinct distribution of the lymphatic capillaries in the periodontium of the maxillary and mandibular cheek teeth, two complementary lymphatic drainage pathways are proposed: (1) superficial lymph drainage via the gingiva, emptying into the mandibular lymph nodes; (2) deep lymph drainage via the mandibular and maxillary spongiosa, emptying into the mandibular and retropharyngeal lymph nodes, respectively.  相似文献   

Natural revegetation of indigenous roadside vegetation by propagules from topsoil   总被引：2，自引：0，他引：2  

Astrid Brekke Skrindo  Per Anker Pedersen 《Urban Forestry & Urban Greening》2004,3(1):29-37

Road construction degrades large areas of indigenous vegetation. Better understanding of ecosystem restoration after degradation will require development and assessment of improved restoration techniques. The potential of natural revegetation based on propagules from topsoil as a restoration technique, to achieve succession towards indigenous vegetation, is not fully understood and accepted as an alternative method for vegetation establishment. The aim of this study was to evaluate this restoration technique by comparing different topsoils with respect to their potentials for revegetation and to describe the early succession on these topsoils by recording change in vegetation cover and the number of species.The study road RV23 is situated in south-east Norway and runs mainly through mixed, mostly coniferous forest. The topsoil (the upper 30 cm) was removed before road construction started, stockpiled during the construction period and then redistributed on the road verge afterwards. In 1999, 10 macroplots of 25 m² were placed randomly on selected topsoils of different qualities, to represent the soil variation in the study area. The soil quality varied from organic-rich to organic-poor soils. Each macro plot contained four 1-m² mesoplots in which the species composition was recorded in 2000 and 2001.Univariate statistical tests were applied to reveal change in vegetation cover between macroplots within 1 year and between years 2000 and 2001. Furthermore, statistical tests were used to reveal the change in single-species frequencies between the study years.Two years after deposition of topsoils, the vegetation cover was generally satisfactory from an aesthetical point of view. However, the variation in vegetation cover and species composition was considerable on different topsoils, reflecting both the local variation of the indigenous vegetation and the soil quality. A total of 121 species of vascular plants were recorded in all 10 macroplots, although not more than 59 species were found in one macroplot. Species composition and single-species frequencies changed considerably from the first year to the second. Among the 61 species that were recorded in more than five mesoplots, the frequency of 16 species increased and the frequency of 10 species decreased significantly (P<0.05). Most of the decreasing species can be considered as weed species and are not represented in the present indigenous vegetation, while most of the increasing species are. The vegetation change from 2000 to 2001 apparently represents the first steps in a succession towards an ecosystem dominated by species of the indigenous vegetation.  相似文献   

A NEW TYPE OF RADIOGRAPHIC BONE REMODELING IN THE DISTAL RADIAL AND ULNAR METAPHYSIS IN 54 NEWFOUNDLAND DOGS     

Cathrine Trangerud  Ronald D. Sande  A. Magnus Rorvik  Astrid Indrebo  Jorunn Grondalen 《Veterinary radiology & ultrasound》2005,46(2):108-113

In this longitudinal observational study, 118 growing Newfoundland dogs were followed with sequential radiographic examination of the right front limb, which were scheduled at ages 3, 4, 6, 12, 18, and 24 months to record evidence of skeletal changes. All affected dogs had evidence of changes at 6 months and dogs included in this study had radiographic examination at 6 months and at least two other scheduled examinations that included either 4 or 12 months. The dogs were privately owned and had individualized nutrition and environment with no maintenance protocol required by the project leaders. Irregularities in bone remodeling in the distal radius and ulna were seen in radiographs of 54 of 118 (45.8%) dogs. These irregularities differ from changes previously described in the Newfoundland dog. The reported irregularities were seen as islands of reduced opacity outlined by thickened, radiopaque osseous trabeculae, which were aligned with the axis of stress. All the 54 dogs with irregularities during some phase of development consistently had changes at 6 months of age. Reorganization of the changes in the bone was slow, and residual changes were visible at 18-24 months of age in many cases. The significance of these findings may lie in their potential for misinterpretation if they had been discovered in the presence of clinical signs such as any lameness or growth arrest. Radiographic appearance indicates disturbances in the bone maturation. The etiopathogenesis is unclear. Nutritional, environmental and genetic factors have to be studied.  相似文献   

Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and Babesia canis rossi by PCR assay     

Duarte SC  Linhares GF  Romanowsky TN  da Silveira Neto OJ  Borges LM 《Veterinary parasitology》2008,152(1-2):16-20

Canine babesiosis is an infectious disease caused by either Babesia gibsoni or Babesia canis protozoans. The latter is also classified under three different phylogenetic groups, referred to as subspecies B. canis canis, B. canis vogeli and B. canis rossi. The objective of the present study was to validate and standardize a PCR assay to discriminate the organisms at the subspecies level. First, the reference sequences of the 18S rRNA, 5.8S rRNA and 28S rRNA genes, including the internal transcribed spacer 1 (ITS1) and 2 (ITS2) of the most common species and subspecies of the genus Babesia were retrieved from the GenBank database. Subspecies-specific primers (BAB3, BAB4 and BAB5) and one genus-specific primer were designed from the alignment of the sequences. The PCR assays were evaluated in three different combinations of primer pairs in order to assure complete specificity for each reaction. The results of the tests had demonstrated effectiveness of the novel primer pairs BAB1/BAB3, BAB1/BAB4 and BAB1/BAB5 for the amplification of the subspecies-specific target fragments of 746 bp (B. c. canis), 546 bp (B. c. vogeli) and 342 bp (B. c. rossi) by PCR. The original enzymatic amplification assays with novel primers reported in this paper were confirmed to be a reliable tool for the specific discrimination among B. canis subspecies by single-step PCR assays.  相似文献   

Improved line weighting reduces seabird bycatch without affecting fish catch in the Brazilian pelagic longline fishery   总被引：1，自引：0，他引：1  

Rodrigo C. Santos  Augusto Silva‐Costa  Rodrigo Sant'Ana  Dimas Gianuca  Oliver Yates  Caio Marques  Tatiana Neves 《水产资源保护:海洋与淡水生态系统》2019,29(3):442-449