Multiplication of Xanthomonas campestris pvs secalis and translucens in host and non-host plants (rye and barley) and development of water soaking1     

F. E. EL-BANOBY  K. W. E. RUDOLPH 《EPPO Bulletin》1989,19(1):105-111

Multiplication of the rye-specific and barley-specific pathovars secalis and translucens, respectively, of Xanthomonas campestris (earlier named Xanthomonas translucens f. sp. cerealis) was determined in two rye and two barley cultivars. Fresh bacterial cultures were suspended in boiled tap water, adjusted to about 3 × 10⁷ cfu ml^?1 and infiltrated into the mesophyll of young leaves. In the compatible combinations, a generation time of 1.7–2.1 h was determined. Significantly higher multiplication rates than in incompatible combinations were not observed before 3 or 4 days after inoculation. At this time, the first small water-soaked areas appeared. Bacteria multiplied up to the seventh day after inoculation, reaching a maximum of at least 10⁹ cells per cm², the same time at which the water-soaked areas had reached their maximum size. Thus, the rate of bacterial multiplication paralleled with the development of the water-soaked areas. In the incompatible combinations, nearly the same generation times were detected during the exponential growth phase. However, water-soaked areas did not appear, and the final bacterial concentration ranged from 1:1000 to 1:100 of that determined in compatible combinations. A distinct early lag phase, characterized by a decrease of the bacterial population down to 1:100 of the starting concentration, was detected in all cases.  相似文献   

Book Reviews-Resolving the challenge posed by agrobiodiversity and plant genetic resources – an attempt. K. Hammer. Journal of Agriculture and Rural Development in the Tropics and Subtropics, Beiheft 76. Kassel university press GmbH and 2004 (‘2003’), (www.upress.uni-kassel.de), 184 pp., ISBN: 3-89958-056-7.     

A. T. Szabó 《Genetic Resources and Crop Evolution》2005,52(3):347

  相似文献   

Determination of sulfamethazine and trimethoprim in liquid feed premixes by HPLC and diode array detection, with an analysis of the uncertainty of the analytical results.     

B Cancho Grande  M S García Falcón  M Rodríguez Comesa?a  J Simal Gándara 《Journal of agricultural and food chemistry》2001,49(7):3145-3150

Sulfamethazine (SMZ) and trimethoprim (TMP) are antibacterials used in veterinary practice. This paper describes a method for their determination in veterinary liquid feed premixes that is based on liquid chromatography with diode array detection. Gradient elution with methanol and ammonium acetate achieved excellent separation of the two analytes within 15 min without any interference from the matrix. Absorbance of the column effluent was monitored at 264 nm for SMZ and at 230 nm for TMP. Detailed analyses of the uncertainties of determinations afford estimated expanded uncertainties of, respectively, 0.2 and 0.1 w/v % for typical SMZ and TMP concentrations of 10.7 and 2.1 w/v %, respectively. At the lower end of the calibrated range of the method, the dominant source of uncertainty is the preparation of standards and the construction of the calibration line.  相似文献   

油脂在提高公猪生产力和生育力上的潜在作用     

潘雪男  P.C.Penny  R.C.Noble  A.Maldjian  S.Cerolin 《国外畜牧学(猪与禽)》2001,(2):24-29

精子及其相关精浆中所含的脂肪成分在组成和脂肪酸成分上是非常独特的。长链多不饱和脂肪酸 (即具有 2 0或 2 2个碳原子并含三个以上双键的脂肪酸 )在绝大多数主要组织所含脂肪中的含量非常有限 ,但在精子和精浆中的含量相当高 ,并具有明显的种属特异性。油脂的这种特性现在日益被认为是使受精最终成功所特别需要的。本综述根据最近一的些研究结果撰写而成 ,从精液质量和功能的一系列决定因子的角度论述了使现代种公猪精子中脂肪 /脂肪酸参数最佳化的可能性 ,可见人们对一系列参数所作的一系列重要研究 ,产生了使公猪和母猪的繁殖能力都得到提高的高度有益结果。文章还从主要的营养学特性和化学特性方面对研究结果进行了讨论 ,并与其它家畜进行了比较 ,还讨论了其对猪繁育部门可能的商业应用价值。  相似文献   

Effect of folic acid supplements on homocysteine concentration in plasma of gestating sows.     

B Barkow  K Pietrzik  G Flachowsky 《Archives of Animal Nutrition》2001,54(1):81-85

An experiment comprising 19 German Landrace sows was established to evaluate the effect of folic acid supplements (10 mg/kg concentrate) on homocysteine and folic acid concentration in plasma and serum, respectively, of highly pregnant sows as compared to an unsupplemented control (basal diet contained 0.62 mg folic acid/kg concentrate). Blood samples were taken between day 75 and 110 of gestation for homocysteine analysis and on day 100 of gestation for folic acid determination. Due to the folic acid supplements serum folic acid concentration increased significantly (104 nmol/l in controls and 140 nmol/l in supplemented sows). In contrast, homocysteine concentration in the plasma was not significantly influenced by folic acid supplements (16.6 mumol/l in controls and 15.2 mumol/l in supplemented sows). Further investigations seem to be necessary to clarify the physiology of homocysteine metabolism in swine.  相似文献   

Concurrent cholangiocarcinoma, peritonitis, paratuberculosis, and aspergillosis in a goat.          下载免费PDF全文

M C Domínguez  G Chvez  F J Trigo    M L Rosales 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(11):884-885

  相似文献   

Use of an antineoepitope antibody for identification of type-II collagen degradation in equine articular cartilage.     

R C Billinghurst  E M Buxton  M G Edwards  M S McGraw  C W McIlwraith 《American journal of veterinary research》2001,62(7):1031-1039

OBJECTIVE: To develop an antibody that specifically recognizes collagenase-cleaved type-II collagen in equine articular cartilage. SAMPLE POPULATION: Cartilage specimens from horses euthanatized for problems unrelated to the musculoskeletal system. PROCEDURE: A peptide was synthesized representing the carboxy- (C-) terminus (neoepitope) of the equine type-II collagen fragment created by mammalian collagenases. This peptide was used to produce a polyclonal antibody, characterized by western analysis for reactivity to native and collagenase-cleaved equine collagens. The antibody was evaluated as an antineoepitope antibody by ELISA, using peptides +/- an amino acid at the C-terminus of the immunizing peptide. Collagen cleavage was assayed from equine articular cartilage cultured with interleukin-1 (IL-1), +/- a synthetic MMP inhibitor, BAY 12-9566. Cartilage specimens from osteoarthritic and nonarthritic joints were compared for antibody staining. RESULTS: An antibody, 234CEQ, recognized only collagenase-generated 3/4-length fragments of equine type-II collagen. This was a true antineoepitope antibody, as altering the C-terminus of the immunizing peptide significantly decreased competition for binding in an inhibition ELISA. The IL-1-induced release of type-II collagen fragments from articular cartilage was prevented with the MMP inhibitor. Cartilage from an osteoarthritic joint of a horse had increased staining with the 234CEQ antibody, compared with normal articular cartilage. CONCLUSIONS AND CLINICAL RELEVANCE: We generated an antineoepitope antibody recognizing collagenase-cleaved type-II collagen of horses. This antibody detects increases in type-II collagen cleavage in diseased equine articular cartilage. The 234CEQ antibody has the potential to aid in the early diagnosis of arthritis and to monitor treatment responses.  相似文献   

Use of laser photocoagulation for treatment of cutaneous angiomatosis in one dog and two cats.     

G M Peavy  E J Walder  J S Nelson  M Rosenberg 《Journal of the American Veterinary Medical Association》2001,219(8):1094-7, 1073

One dog and 2 cats were evaluated because of multiple progressively enlarging cutaneous vascular plaques. Biopsies were performed and revealed small well-circumscribed dermal nodules of dilated fully enclosed blood-filled spaces lined by single layers of endothelial cells aligned on thin fibrous walls, with minimal mitotic activity. A diagnosis of cutaneous angiomatosis was made in all 3 animals. Cutaneous angiomatosis is a progressive proliferative lesion of vascular tissue involving the dermis and subcutaneous tissues of dogs and cats. Lasers of selected wavelengths have been used to induce photothermal coagulation of hemoglobin-containing cutaneous lesions in humans; argon-pumped dye and neodymium:yttrium-aluminum-garnet lasers were used to treat the cutaneous lesions in the animals of this report. Laser treatment may provide an alternative to wide surgical resection or limb amputation for management of this condition in veterinary patients.  相似文献   

Association between Chlamydia psittaci seropositivity and abortion in Italian dairy cows.     

S Cavirani  C S Cabassi  G Donofrio  B De Iaco  S Taddei  C F Flammini 《Preventive veterinary medicine》2001,50(1-2):145-151

Although the seroprevalence of Chlamydia psittaci is widespread in Italian dairy herds, its role in inducing genital disorders has not been elucidated. We therefore set up a case-control study to compare seroprevalence to C. psittaci in an aborted-cow population and in a randomly selected control group in the province of Parma (the Po Valley of northern Italy). The true seroprevalence (45%) in aborted cows was significantly higher than that in the control group (24%) (adjusted odds ratio=2.53).  相似文献   

Immunohistochemical Detection of Mycoplasma agalactiae in Formalin‐Fixed,Paraffin‐Embedded Tissues from Naturally and Experimentally Infected Goats     

F. RODRÍGUEZ  G. A. RAMÍREZ  A. S. RAMÍREZ  H. J. BALL  A. ESPINOSA DE LOS MONTEROS  A. FERNNDEZ 《Zoonoses and public health》2002,49(5):226-229

Samples from the mammary tissue of 14 lactating goats (12 naturally infected and two experimentally infected) were examined for the presence of Mycoplasma agalactiae. A monoclonal antibody (5G12) was applied to formalin‐fixed, paraffin‐wax‐embedded sections and labelled by the avidin–biotin peroxidase complex (ABC) method. Histological examination of tissue sections revealed strong immunoreactivity in all animals included in the study. Mycoplasma agalactiae antigen was mainly detected in the cellular debris at the periphery of purulent exudates present within lactiferous sinuses, and lactiferous and interlobular ducts. In addition, M. agalactiae organisms appeared in the cytoplasm of the epithelium of ducts, and in infiltrating macrophages and neutrophils within the ducts, alveoli, interstitial tissue and regional lymph node sinuses. It is concluded that this monoclonal antibody‐based immunohistochemical technique is an efficient and specific method for the post‐mortem detection of M. agalactiae in cases of clinical mastitis as well as being a useful tool for the study of the route of infection and cellular types involved during mastitis caused by this organism.  相似文献