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41.
Trans-endocytosis of CD80 and CD86: a molecular basis for the cell-extrinsic function of CTLA-4 总被引:1,自引:0,他引:1
Qureshi OS Zheng Y Nakamura K Attridge K Manzotti C Schmidt EM Baker J Jeffery LE Kaur S Briggs Z Hou TZ Futter CE Anderson G Walker LS Sansom DM 《Science (New York, N.Y.)》2011,332(6029):600-603
Cytotoxic T lymphocyte antigen 4 (CTLA-4) is an essential negative regulator of T cell immune responses whose mechanism of action is the subject of debate. CTLA-4 shares two ligands (CD80 and CD86) with a stimulatory receptor, CD28. Here, we show that CTLA-4 can capture its ligands from opposing cells by a process of trans-endocytosis. After removal, these costimulatory ligands are degraded inside CTLA-4-expressing cells, resulting in impaired costimulation via CD28. Acquisition of CD86 from antigen-presenting cells is stimulated by T cell receptor engagement and observed in vitro and in vivo. These data reveal a mechanism of immune regulation in which CTLA-4 acts as an effector molecule to inhibit CD28 costimulation by the cell-extrinsic depletion of ligands, accounting for many of the known features of the CD28-CTLA-4 system. 相似文献
42.
Mangal Deep Tuti Ramesh Singh Pal Dibakar Mahanta Brij Mohan Pandey Jaideep Kumar Bisht 《Communications in Soil Science and Plant Analysis》2020,51(7):948-962
ABSTRACTLack of crop diversification with suitable vegetable-based cropping system is a major constraint in limiting the productivity and sustainability of north-western Indian sub-Himalayas. To find out a sustainable vegetable-based cropping system in this region, a three year colocasia-based vegetable intensive experiment was conducted at Hawalbagh, Almora, India in a sandy clay loam soil under sub-temperate climatic conditions. Seven colocasia-based vegetable cropping systems along with rice–wheat system were compared under recommended package and practices. The system productivity in terms of colocasia equivalent yield was highest under colocasia–onion–frenchbean (52.38 Mg ha?1) system. Sustainable yield index was highest with colocasia–gardenpea–frenchbean system (0.86). After 3 years, total soil organic carbon (0–5%), available N (2–22%), P (–7% to 14%) and K (3–15%) concentrations were increased in all cropping systems except rice–wheat system, where negative balance of available P (7%) was observed over that of initial soil. Significantly higher soil microbial activity, soil carbohydrate, dehydrogenase, protease, acid and alkaline phosphatases activity were observed under colocasia–onion system. The results suggest that colocasia–onion–frenchbean system with higher productivity improves soil fertility and enhances enzymatic activities. 相似文献
43.
Chaulya S. K. Chakraborty M. K. Ahmad M. Singh R. S. Bondyopadhay C. Mondal G. C. Pal D. 《Water, air, and soil pollution》2002,140(1-4):21-55
All major mining activities, particularly opencast coal mining,directly or indirectly contribute to the problem of air pollution.Therefore, air quality assessment and prediction arerequired to prevent and minimize the air quality deteriorationdue to various opencast coal mining operations. Determination ofemission rates for these activities is the first and foremostconcern. In view of the above, this study was undertaken to determinate emission rates and to develop empericalformulae to calculate emission rates of various opencast coalmining activities.To achieve the objectives, seven coal mining sites were selectedto generate site-specific emission data by considering miningpractices, method of working, geographical location,accessibility and above all resourceavailability. The study covers various mining activities andlocations including drilling, overburden loading and unloading,coal loading and unloading, the coal handling plant, the exposedoverburden dump, the stock yard, the workshop, the exposed pit surface roadsand haul roads.Based on the study, a set of twelve emperical formulae have beendeveloped for calculation of suspended particulate matter (SPM)emission rates from various opencast coal mining activities. Theemission of gaseous pollutants (sulphur dioxide and nitrogenoxide) has been found negligible for various mining activities.Therefore, observation for gaseous pollutants has been consideredfor overall mine activities and empirical formulae have subsequently beendeveloped.The developed empirical formulae were calculatedby a field study at another coal mine. The measured andcalculated values of emission rate were compared for eachactivity. Average correlation between the measured andcalculated values for different activities was estimated to be 85.6–99.9%, which indicates fairly good accuracy.Validation of the study was also carried out by means of thefugitive dust model (FDM) using the calculated emissionrate data from the empirical formulae for each mining activity of amine, meteorological data and other details as input.The average accuracy between measured and predictedvalues of concentration of SPM at certain receptor locations wasfound to be 79%. A user-friendly emission software called`EmissCalc' was developed to calculate emission rate, whichcan be used as input for different air quality models. 相似文献
44.
M. Das P. H. Zaidi M. Pal & U. K. Sengupta 《Journal of Agronomy and Crop Science》2002,188(4):219-224
An experiment was undertaken using open‐top chambers to study the effect of CO2 enrichment on crop growth and to assess the variation related to the time of CO2 exposure. In general, enriched CO2 conditions (600 ± 50 p.p.m.) in the chamber positively affected the growth and development of mungbean (Vigna radiata), a short‐duration (60–65 days) summer pulse crop. However, there was significant variability in the effect of the crop stage/time of CO2 exposure. It was observed that high CO2 exposure at an early growth stage [0–20 days after germination (DAG)] had a larger effect than that at a later growth stage (21–40 DAG). The experiment also provided a means of assessing the short‐ and long‐term effects of elevated CO2 on the carbon exchange rate at both stages of exposure. The study revealed that the sensitivity of plants to high CO2 was more pronounced with respect to net photosynthetic rate. The overall photosynthetic activity gave greater growth and development of plants under high CO2. 相似文献
45.
The occurrence and aetiological significance of Aspergillus fumigatus, an opportunistic pathogen, have been studied in 93 animals with various ophthalmological problems. A total of 93 eye swabs collected from 35 mules, 26 dogs, 13 fowl, 11 cattle, five buffaloes and three camels were investigated mycologically for the presence of A fumigatus. The pathogen was isolated in pure and heavy growth from the swabs from two dogs, one bull, one mule and one fowl. The fungus was also demonstrated directly in clinical material by the potassium hydroxide technique. A fumigatus could not be cultured from the buffaloes and camels. All the five cases had been treated with broad spectrum antibiotics and cortisone and two had received traumatic injury to the eyes (one mule and a bull). The organism was not isolated in pure culture from the conjunctival swabs of 22 apparently healthy animals (11 dogs, six mules, three fowl and two cattle). Many other saprophytic fungi were recovered in mixed cultures but were considered to be contaminants. The clinical signs and diagnostic criteria of oculomycosis have been discussed. 相似文献
46.
U. R. Pal B. A. Kalu J. C. Norman D. K. Adedzwa 《Journal of Agronomy and Crop Science》1988,160(2):132-140
Field experiments were conducted to investigate into the N and P fertilizer use in soybean/maize mixture during the wet seasons of 1985 and 1986 at Yandev Agricultural Experiment Station. Soybean/maize mixtures with 2/3 of the respective plant population of both crops in pure stands were raised at factorial combinations of four rates of N, 0, 40, 80, and 120 kg N/ha and four rates of P, 0, 13.2, 26.4 and 39.6 kg P/ha in a randomized complete block design with three replications.
The grain yield of maize cv. TZB (a pure line variety) in mixture and land equivalent ratio (LER) increased linearly up to 120 kg N/ha in 1985 as against significant increase in the grain yield of maize cv. 8322–3 (a hybrid variety) and LER up to 40 kg N/ha only in 1986 primarily due to greater fertilizer N recovery by the latter. Significant enhancement in LER was also recorded up to 26.4 kg P/ha in both years. The yield of soybean in mixture remained unaffected by N or P rates.
Total N uptake by shoots of soybean/maize mixture was significantly and linearly increased up to the application of 120 kg N/ha in 1985 as against significant increase in total N uptake by soybean/maize mixture only up to 40 kg N/ha in 1986 owing to the greater fertilizer N recovery by maize (69%) in the latter than the one (29.75—31.75%) in the former. Total N uptake by shoots of soybean/maize was also increased significantly due to the application of 26.4 kg P/ha.
Application of N and P also significantly enhanced P uptake by shoots of soybean/maize mixture and fertilizer P recovered by mixture was less (3.99—12.95%) in 1985 than the one (19.95—32.12%) in 1986. 相似文献
The grain yield of maize cv. TZB (a pure line variety) in mixture and land equivalent ratio (LER) increased linearly up to 120 kg N/ha in 1985 as against significant increase in the grain yield of maize cv. 8322–3 (a hybrid variety) and LER up to 40 kg N/ha only in 1986 primarily due to greater fertilizer N recovery by the latter. Significant enhancement in LER was also recorded up to 26.4 kg P/ha in both years. The yield of soybean in mixture remained unaffected by N or P rates.
Total N uptake by shoots of soybean/maize mixture was significantly and linearly increased up to the application of 120 kg N/ha in 1985 as against significant increase in total N uptake by soybean/maize mixture only up to 40 kg N/ha in 1986 owing to the greater fertilizer N recovery by maize (69%) in the latter than the one (29.75—31.75%) in the former. Total N uptake by shoots of soybean/maize was also increased significantly due to the application of 26.4 kg P/ha.
Application of N and P also significantly enhanced P uptake by shoots of soybean/maize mixture and fertilizer P recovered by mixture was less (3.99—12.95%) in 1985 than the one (19.95—32.12%) in 1986. 相似文献
47.
Various enzymes of the tricarboxylic acid cycle (TCA) viz., aconitase (E.C. 4.2.1.3), isocitrate dehydrogenase (E.C. 1.1.1.42), succinate dehydrognease (E.C. 1.3.99.1), fumarate reductase (NADH: fumarate oxido-reductase), fumarase (E.C. 4.2.1.2) and maltate dehydrogenase (E.C. 1.1.1.37) were detected in adult Haemonchus contortus (Nematoda: Trichostrongylidae), in vitro. Low activities of aconitase and isocitrate dehydrogenase suggested that the TCA cycle has a minor function and the pathway of CO2 fixation is the major pathway in the energy metabolism of the parasite. In vitro incubation in Tyrode's solution had no significant effect on TCA cycle enzymes and the worm was able to maintain normal metabolism for 12 h.The effects of dl-tetramisole and rafoxanide on various enzymes of the TCA cycle were studied in adult H. contortus. At 50 μg ml?1 varying degrees of succinate dehydrogenase and fumarate reductase activities were observed. At the same concentration, the activities of other enzymes remained unaltered. 相似文献
48.
Allelopathic effects of aqueous extract of three weeds, Ageratum conyzoides L., Imperata cylindrica (L.) Beauv and Commelina benghalensis L. of Nigerian savanna were investigated on the germination and seedling vigour of soybean ( Glycine max [L.] Merr.) and maize ( Zea mays L.). Aqueous extracts of these weeds exhibited allelopathic phenomenon on seed germination and seedling vigour. Aqueous extracts of I. cylindrica and C. benghalensis at 1 % and 5 % concentration (w/v) had stimulatory effect on soybean germination as against inhibitory effect of 10 % aqueous extract of all weeds on the germination of both soybean and maize seeds. Seedling vigour-length of roots and shoots and fresh weight of seedlings were promoted by aqueous extract of A. conyzoides up to 5 % concentration in soybean whereas all concentrations of extract stimulated the seedling vigour of maize. Aqueous extract of I. cylindrica up to 5 % also caused stimulatory effect on the seedling vigour of both crops but such effects were lesser in magnitude than that observed in case of A. conyzoides. On the contrary, the aqueous extract of C. benghalensis at all concentrations resulted in deleterious effect on the seedling vigour of both soybean and maize. 相似文献
49.
50.
Zimmerman KL Wilcke JR Robertson JL Feldman BF Kaur T Rees LR Spackman KA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2005,34(1):7-16
BACKGROUND: The Systematized Nomenclature of Medicine (SNOMED) is an established standard nomenclature for the expression of human and veterinary medical concepts. Nomenclature standards ease sharing of medical information, create common points of understanding, and improve data aggregation and analysis. OBJECTIVES: The objective of this study was to determine whether SNOMED adequately represented concepts relevant to veterinary clinical pathology. METHODS: Concepts were isolated from 3 different types of clinical pathology documents: 1) a textbook (Textbook), 2) the Results sections of industry pathology reports (Findings), and Discussion sections from industry pathology reports (Discussion). Concepts were matched (mapped) by 2 reviewers to semantically-equivalent SNOMED concepts. A quality score of 3 (good match), 2 (problem match), or 1 (no match) was recorded along with the SNOMED hierarchical location of each mapped concept. Results were analyzed using Cohen's Kappa statistic to assess reviewer agreement and chi-square tests to evaluate association between document type and quality score. RESULTS: The percentage of good matches was 48.3% for the Textbook, 45.4% for Findings, and 47.5% for Discussion documents, with no significant difference among documents. Of remaining concepts, 40% were partially expressed by SNOMED and 14% did not match. Mean reviewer agreement on quality score assignments was 76.8%. CONCLUSIONS: Although SNOMED representation of veterinary clinical pathology content was limited, missing and problem concepts were confined to a relatively small area of terminology. This limitation should be addressed in revisions of SNOMED to optimize SNOMED for veterinary clinical pathology applications. 相似文献