Complete genome sequence and lytic phase transcription profile of a Coccolithovirus   总被引：2，自引：0，他引：2  

Wilson WH  Schroeder DC  Allen MJ  Holden MT  Parkhill J  Barrell BG  Churcher C  Hamlin N  Mungall K  Norbertczak H  Quail MA  Price C  Rabbinowitsch E  Walker D  Craigon M  Roy D  Ghazal P 《Science (New York, N.Y.)》2005,309(5737):1090-1092

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Efficacy of preanesthetic intramuscular administration of ephedrine for prevention of anesthesia-induced hypotension in cats and dogs          下载免费PDF全文

Christine Egger  Mary-Ann McCrackin  Erik Hofmeister  Gwenola Touzot-Jourde    Bart Rohrbach 《The Canadian veterinary journal. La revue veterinaire canadienne》2009,50(2):179-184

To determine if the preanesthetic administration of ephedrine would prevent anesthesia-induced hypotension in dogs and cats, 10 cats were anesthetized with acepromazine, butorphanol, ketamine, and isoflurane, and 8 dogs were anesthetized with acepromazine, morphine, propofol, and halothane. Cats received ephedrine or saline 10 minutes after premedication. Dogs received ephedrine or saline at the time of premedication. Systolic arterial blood pressure, respiratory rate, heart rate, end-tidal CO₂, O₂ saturation, cardiac rhythm, and rectal temperature were recorded.  相似文献   

Fertilization efficiency of cryopreserved sperm from striped catfish, Pangasius hypophthalmus (Sauvage)     

Samorn Kwantong  & Amrit N Bart 《Aquaculture Research》2009,40(3):292-297

The fertilization efficiency of cryopreserved sperm was compared with fresh sperm from striped catfish, Pangasius hypophthalmus . Of the two sets of experiments carried out, the first compared four sperm doses using fresh sperm and fresh eggs. The second experiment compared six concentrations of cryopreserved sperm ranging from 6.94 × 10⁷ to 6.94 × 10¹⁰ to fertilize 100 eggs per batch. Fertilization, hatch and survival rates were compared between cryopreserved and fresh sperm. The highest fertilization rate (53.75±1.62%) was achieved with a sperm dose of 6.94 × 10⁸. Increasing the sperm dose to 3.47 × 10⁹ did not increase the fertilization rate, indicating that the optimum sperm:egg ratio lies between 6.94 × 10⁶ and 3.47 × 10⁷ sperm per egg. Both highest (6.94 × 10¹⁰) and the lowest (6.94 × 10⁷) sperm doses resulted in lower fertilization rates (2.04% and 16.90% respectively). No significant differences were found among four fresh sperm doses compared. Mean hatch and survival rates resulting from fresh and cryopreserved sperm were similar. The experiment shows that while only 1.89 × 10⁶ fresh spermatozoa was required to fertilize a fresh egg, 6.94 × 10⁶ (or 3.67 times more) cryopreserved sperm was required to achieve the same level of fertilization. This provides important information for making decision to cryopreserve sperm for commercial and/or conservation purposes.  相似文献   

Contrast-enhanced 3D micro-CT of plant tissues using different impregnation techniques     

Zi Wang  Pieter Verboven  Bart Nicolai 《Plant methods》2017,13(1):105

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Demonstration and validation of enhanced monitored natural recovery at a pesticide-contaminated sediment site     

Fetters  Kyle  Rosen  Gunther  Kirtay  Victoria  Chadwick  Bart  Conder  Jason  Sacks  Victoria Paris  Grover  Melissa  Magar  Victor 《Journal of Soils and Sediments》2020,20(1):204-219

Purpose

Monitored natural recovery (MNR) combined with a thin-layer cap (TLC), often referred to as enhanced monitored natural recovery (EMNR), has the potential to accelerate and improve the effectiveness of MNR as a remedial strategy while minimizing widespread disturbance to the existing habitat. This study evaluated the effectiveness of a nominal 15-cm thin-layer sand cap as an EMNR remedial strategy to address sediments that were moderately contaminated with the chlorinated pesticide dichlorodiphenyltrichloroethane and its derivatives, collectively DDX.

Materials and methods

Physical, chemical, and biological measurements were conducted pre-remedy placement and 2, 14, and 25 months post-placement. Measurements were used to evaluate (1) TLC stability; (2) bottom-up mixing of the TLC; (3) advection through the TLC; (4) characteristics of newly deposited sediment atop the EMNR layer compared to pre-remedy surface sediment conditions; (5) changes in contaminant bioavailability; and (6) physical impacts to the benthic community.

Results and discussion

Significant reductions were observed from measurements conducted pre- and post-placement in surface sediment (84–97%), porewater (33–75%), and tissue concentrations (Lumbriculus variegatus deployed in situ) (72–82%). A 63 to 72% decrease in DDX depositional mass flux also was observed. Multiple lines of evidence indicated that the TLC material remained stable. Deposition of suspended material with low concentrations of DDX influenced low concentrations in the surface sediments. No adverse effects were observed on the benthic invertebrate community after TLC placement, and ecological metrics indicated increases in benthic community health, even in the short time period (2 months) following TLC placement.

Conclusions

This demonstration showed that EMNR can be effective at reducing biological exposure in surface sediments while minimizing short-term disturbances to benthic communities at sites where MNR is a remedy option, but natural deposition rates are inadequate to achieve cleanup goals within a reasonable timeframe.

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Book reviews     

Frank Bart Lucarelli Jr.  V. Kovda  H. Horvath  Michael Waldichuk  K. C. Pillai 《Water, air, and soil pollution》1978,9(1):123-128

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Salinity effects on reproduction of giant freshwater prawn Macrobrachium rosenbergii (de Man)     

Pham Truong Yen  Amrit N. Bart   《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):124-128

Understanding the role of salinity in breeding and growth has the potential to enhance production of giant freshwater prawn, Macrobrachium rosenbergii. This study investigated the reproduction of females reared in salinities of 0, 6, 12, and 18 g L^− 1. Mean weight of females decreased with increased salinity (31.40 ± 1.54, 25.14 ± 1.16, 20.80 ± 0.81, and 16.62 ± 1.04 g at 0, 6, 12, and 18 g L^− 1, respectively). Larval production was delayed by 2 months in females reared in 12 g L^− 1 compared to 6 and 0 g L^− 1 and the cumulative number of berried females decreased with increased salinity. The number of larvae produced was positively correlated to weight of female. A larval production not only differed between salinity treatments, but also that larval production per gram of female differed between treatments. Females reared in lower salinity of 0 and 6 g L^− 1 produced larger numbers of larvae (12,155 ± 480 and 6519 ± 323, respectively) compared to 12 and 18 g L^− 1 (3751 ± 256 and 0, respectively). The number of larvae produced per gram of female was inversely related to the salinity levels (Y = − 37.54X + 685.65, n = 339, r² = 0.995, p < 0.05). Survival of larvae from females reared at 0 and 6 g L^− 1 was higher than those from females reared in 12 g L^− 1. This study clearly shows that female broodstock reared in lower salinity was larger, reproduced early, and produced more offspring than at higher salinity and this could significantly impact coastal prawn culture where seasonal fluctuation of salinity in the hatchery is common.  相似文献   

Phylogeography and conservation gaps of Musa balbisiana Colla genetic diversity revealed by microsatellite markers     

Mertens  Arne  Bawin  Yves  Vanden Abeele  Samuel  Kallow  Simon  Swennen  Rony  Vu  Dang Toan  Vu  Tuong Dang  Minh  Ho Thi  Panis  Bart  Vandelook  Filip  Janssens  Steven B. 《Genetic Resources and Crop Evolution》2022,69(7):2515-2534

Genetic Resources and Crop Evolution - Collection and storage of crop wild relative (CWR) germplasm is crucial for preserving species genetic diversity and crop improvement. Nevertheless, much of...  相似文献